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Biocompatibility of Human Auricular Chondrocytes Cultured onto a Chitosan/Polyvynil Alcohol/Epichlorohydrin-Based Hydrogel for Tissue Engineering Application International Journal of Morphology
Melgarejo-Ramírez,Yaaziel; Sánchez-Sánchez,Roberto; García-Carvajal,Zaira; García-López,Julieta; Gutiérrez-Gómez,Claudia; Luna-Barcenas,Gabriel; Ibarra,Clemente; Velasquillo,Cristina.
Tissue engineering (TE) has become an alternative for auricular reconstruction based on the combination of cells, molecular signals and biomaterials. Scaffolds are biomaterials that provide structural support for cell attachment and subsequent tissue development. Ideally, a scaffold should have characteristics such as biocompatibility and bioactivity to adequate support cell functions. Our purpose was to evaluate biocompatibility of microtic auricular chondrocytes seeded onto a chitosan-polyvinyl alcohol-epichlorohydrin (CS-PVA-ECH) hydrogel to propose this material as a scaffold for tissue engineering application. After being cultured onto CS-PVA-ECH hydrogels, auricular chondrocytes viability was up to 81%. SEM analysis showed cell attachment and...
Tipo: Journal article Palavras-chave: Elastic cartilage; Auricular reconstruction; Chitosan hydrogel; Tissue engineering; Biocompatibility.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400036
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Biocompatibility, osteointegration, osteoconduction, and biodegradation of a hydroxyapatite-polyhydroxybutyrate composite BABT
Reis,Emily Correna Carlo; Borges,Andréa Pacheco Batista; Fonseca,Cláudio César; Martinez,Mastoby Miguel Martinez; Eleotério,Renato Barros; Morato,Gláucia Oliveira; Oliveira,Paulo Miranda.
In this work, biocompatibility, osteointegration, osteoconductivity, and biodegradation of a hydroxyapatite polyhydroxybutyrate new composite were evaluated. The composite was implanted in rabbits' bone defects and clinical, radiographic, histological, and histomorphometric data of these animals were compared with those of unfilled defects on the days 8th, 45th, and 90th after surgery. No significant differences existed between the groups for the evaluated clinical parameters. Radiographs showed bone-composite direct contact. Bone formed within the defect, interface and inside the composite. Significant differences were found between the bone and connective tissues percentage within the defect at all dates and at the interface on the 45th day, bone tissue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomaterials; Bone; Biocompatibility; Biodegradation; Hydroxyapatite-polyhydroxybutyrate composite.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400010
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Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair BABT
Vora,Shruti Dineshbhai; Kumar,Vineet; Asodiya,Foram Arvindbhai; Singh,Vivek Kumar; Fefar,Dhaval Tribhovanbhai; Gajera,Harsukh Popatbhai.
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocompatibility; Bubaline diaphragm matrix; DNA quantification; Spectroscopy; Scanning electron microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100309
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Comparison of subcutaneous inflammatory response to commercial and engineered zinc hydroxyapatite implants in rabbits Arq. Bras. Med. Vet. Zootec.
Muñoz,L.C.; Cardona.-Ramirez,S.; Silva,R.F..
ABSTRACT Hydroxyapatite (HA) is widely used as a biomaterial for bone repair and metallic prostheses coating. The main limitations of the current commercial synthetic hydroxyapatite compounds include high cost and decreased availability, especially for veterinary medicine purposes. Additionally, it is thought that HA biocompatibility and bioactivity could be enhanced by the addition of metal compounds. The objective of this work was to compare the subcutaneous tissue response of commercial and engineered hydroxyapatite obtained from the bovine femur diaphysis mixed with different concentrations of hexa-hydrated Zinc Nitrate in rabbits. Twenty-Five New Zealand female rabbits were used. Five treatments were done according to HA composition (commercial HA, no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal model; Biocompatibility; Bone; Integration.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601873
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Evaluation of different adjuvants formulations for bluetongue vaccine BABT
Macedo,Ludmila Branco; Lobato,Zélia Inês Portela; Fialho,Sílvia Ligório; Viott,Aline de Marco; Guedes,Roberto Maurício Carvalho; Silva-Cunha,Armando.
This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Antibodies titers were determined by serum-neutralization test. Histological analyses of lesions at the site of adjuvants application were done. Results showed that multiple emulsion and microemulsion maintained their stability even in the presence of complex components and presented adequate characteristics for subcutaneous administration. They were able to induce immune response against BTV, but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Emulsion; Microemulsion; Vaccine adjuvants; Immunology; Biocompatibility.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600007
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Hydroxyapatite, polycaprolactone and alendronate composites for bone regeneration in rabbits' olecranon: histological features Arq. Bras. Med. Vet. Zootec.
Valente,F.L.; Reis,E.C.C.; Sepúlveda,R.V.; Ochoa,C.C.R.; Santos,L.C.; Corsini,C.M.; Borges,A.P.B..
O presente trabalho teve como objetivo avaliar a biocompatibilidade e a osseointegração de compósitos de hidroxiapatita (HA), policaprolactona (PCL) e alendronato (ALN) em defeitos ósseos produzidos no olécrano de coelhos. Trinta e seis coelhos foram distribuídos em quatro grupos, recebendo como tratamento: (1) compósito de HA (49,5%), PCL (49,5%) e ALN (1%); (2) HA (50%) e PCL (50%); (3) PCL (100%); e (4) solução salina 0,9%. As amostras para análise histológica foram coletadas de três animais de cada grupo aos oito, 45 e 90 dias de pós-operatório. No oitavo dia, as bordas do defeito ainda eram identificáveis em todos os grupos. Tecido ósseo novo era formado em contato com o biomaterial apenas nas formulações que incluíam HA. Essas características...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bone regeneration; Biocompatibility; Biomaterial.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200543
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Hydroxyapatite-lignin composite as a metallic implant-bone tissue osseointegration improver: experimental study in dogs Ciência Rural
Valente,Fabrício Luciani; Santos,Letícia Corrêa; Sepúlveda,Rodrigo Viana; Gonçalves,Gustavo Pereira; Eleotério,Renato Barros; Reis,Emily Correna Carlo; Borges,Andréa Pacheco Batista.
The study aimed to evaluate biocompatibility, osteoconduction and osseointegration of a pasty composite of hydroxyapatite (20%) and lignin (80%) as a promoter of metal implant and bone tissue integration. An intramedullary Schanz pin was implanted in both tibias of fifteen bitches. In the left tibia, the pin was coated with the biomaterial at the time of surgery. Marrow cavity was also filled with the biomaterial. Right limb did not receive the biomaterial, then constituting the control group. Tibias were harvested from five animals at 8, 60 and 150 days after surgery; three of them were analyzed by histological and biomechanical assessment and the two remaining tibias by X-ray diffraction. Results showed that the biomaterial is biocompatible, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomaterial; Biocompatibility; Osseointegration; Osteoconduction; Tissue regeneration.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200324
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Improved biological performance of magnesium by micro-arc oxidation BJMBR
Ma,W.H.; Liu,Y.J.; Wang,W.; Zhang,Y.Z..
Magnesium and its alloys have recently been used in the development of lightweight, biodegradable implant materials. However, the corrosion properties of magnesium limit its clinical application. The purpose of this study was to comprehensively evaluate the degradation behavior and biomechanical properties of magnesium materials treated with micro-arc oxidation (MAO), which is a new promising surface treatment for developing corrosion resistance in magnesium, and to provide a theoretical basis for its further optimization and clinical application. The degradation behavior of MAO-treated magnesium was studied systematically by immersion and electrochemical tests, and its biomechanical performance when exposed to simulated body fluids was evaluated by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Magnesium; Micro-arc oxidation; Biodegradable; Biocompatibility; Mechanical properties; Corrosion.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300214
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In vivo biocompatibility of silicon dioxide nanofilm used as antimicrobial agent on acrylic surface Anais da ABC (AABC)
LACERDA-SANTOS,ROGÉRIO; LIMA,ANTONIA BÁRBARA L.; PENHA,ELIZANDRA S. DA; SANTOS,ANTONIELSON DOS; CARVALHO,FABÍOLA G.; PITHON,MATHEUS M.; DANTAS,ANTÔNIO FLÁVIO M..
Abstract the focus ofthis study was to testthe hypothesisthatthere would be no difference betweenthe biocompatibility of silicon dioxide nanofilms used as antimicrobial agents. Sixty male Wistar rats were divided into 4 groups (n=15): Group C (Control,Polyethylene), Group AR (Acrylic Resin), Group NP (Acrylic Resin coated with NP-Liquid), Group BG (Acrylic Resin coated with Bacterlon).the animals were sacrificed with 7,15 and 30 days and tissues analyzed as regardsthe events of inflammatory infiltrate, edema, necrosis, granulation tissue, mutinucleated giant cells, fibroblasts and collagen. Kruskal-Wallis and Dunn tests was used (P<0.05). Intense inflammatory infiltrate was shown mainly in Groups BG and AR, with significant difference from Control Group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomaterials; Biocompatibility; Dentistry; Inflammation.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100910
Registros recuperados: 9
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