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Registros recuperados: 77
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A novel "Integrated Biomarker Response" calculation based on reference deviation concept ArchiMer
Sanchez, Wilfried; Burgeot, Thierry; Porcher, Jean-marc.
Multi-biomarker approaches are used to assess ecosystem health and identify impacts of environmental stress on organisms. However, exploration of large datasets by environmental managers represents a major challenge for regulatory application of this tool. Several integrative tools were developed to summarize biomarker responses. The aim of the present paper is to update calculation of the "Integrated Biological Response" (IBR) described by Beliaeff and Burgeot (Environ Toxicol Chem 21:1316-1322, 2002) to avoid weaknesses of this integrative tool. In the present paper, a novel index named "Integrated Biological Responses version 2" based on the reference deviation concept is presented. It allows a clear discrimination of sampling sites as for the IBR, but...
Tipo: Text Palavras-chave: Biomarkers; Data analysis; Graphic tools; Three-spined stickleback.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00139/25069/23191.pdf
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A pollution-monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha) ArchiMer
Minier, Christophe; Abarnou, Alain; Jaouen Madoulet, Agnes; Le Guellec, Anne-marie; Tutundjian, Renaud; Bocquene, Gilles; Leboulenger, François.
Zebra mussel (Dreissena polymorpha) is an invasive species that has proliferated in European and North American rivers and lakes during the last century. In this study, D. polymorpha has been used to provide information on contamination levels and biological effects in the Seine Estuary (France). The bivalves accumulated polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) to a high degree with values reaching 800 ng/g dry weight for PCBs (sum of 20 congeners), and 1,000 ng/g dry weight of PAHs (sum of 14 compounds) in the whole body. These values are among the highest reported of PCBs and, to a lesser extent, of PAHs in other contaminated areas in the world. Toxic equivalent quantities of PCBs and PAHs detected in zebra mussels...
Tipo: Text Palavras-chave: Biomarkers; Multixenobiotic resistance; Polycyclic aromatic hydrocarbons; Polychlorinated biphenyls; Dreissena polymorpha.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2130.pdf
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Active biomonitoring in Greek coastal waters: Application of the integrated biomarker response index in relation to contaminant levels in caged mussels ArchiMer
Tsangaris, Catherine; Hatzianestis, Ioannis; Catsiki, Vassiliki-angelique; Kormas, Konstantinos Ar; Strogyloudi, Evangelia; Neofitou, Christos; Andral, Bruno; Galgani, Francois.
An integrated approach using biomarkers and contaminant levels in mussels Mytilus galloprovincialis L was employed to assess chemical contamination in Greek coastal waters within the framework of the MYTIMED program. Biomarkers (metallothioneins, glutathione S-transferase, catalase, acetylcholinesterase and RNA:DNA ratio) have been previously described in mussels caged at 14 sites in coastal areas influenced by different types of anthropogenic activities. This study applied a biomarker index, the Integrated Biomarker Response (IBR) to summarize biomarker responses and relate stress levels to concentrations of organochlorine compounds (PCBs, DDTs), polycyclic aromatic hydrocarbons (PAHs), and metals (Cu, Ni, Fe, Zn) measured in the mussel tissues. The IBR...
Tipo: Text Palavras-chave: Pollution; Biomarkers; Monitoring; Mussel caging; Mytilus galloprovincialis; Mediterranean Sea.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00059/17033/14718.pdf
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Acute exposure of fish to nano-TiO2 at environmental levels of ultraviolet light. Repositório Alice
CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Dióxido de titânio; Nano-TiO2; Nanotecnologia; Substância tóxica; Impacto ambiental; Peixe; Pacu; Biomarkers; Ecotoxicology; Fish; Nanotechnology; Piaractus mesopotamicus; Titanium dioxide; Ultraviolet radiation.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/981722
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Aflatoxin M1 in the urine of non-carriers and chronic carriers of hepatitis B virus in Maringa, Brazil BJPS
Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel.
Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011
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Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil ArchiMer
Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy.
Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)...
Tipo: Text Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00347/45828/45916.pdf
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Association between syndecan-1 and renal function in adolescents with excess weight: evidence of subclinical kidney disease and endothelial dysfunction BJMBR
Saboia,Z.M.R.M.; Meneses,G.C.; Martins,A.M.C.; Daher,E.F.; Silva Junior,G.B..
Excess weight (overweight and obesity) is associated with kidney and cardiovascular disease. The aim of this study was to investigate the association between syndecan-1 and renal function among adolescents with excess weight. A total of 56 students from a public school at Fortaleza, CE, Brazil, were investigated. The adolescents were submitted to anthropometric evaluation, including weight, height, blood pressure and body mass index. Blood and urine samples were collected for the determination of serum lipids (total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglycerides), and the endothelial injury biomarker syndecan-1. Participants' mean age was 16±1 years (range 14-19 years), and 68% were females. Overweight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obesity; Overweight; Biomarkers; Kidney disease; Endothelium; Adolescents.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300606
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Association of genotypes with viral load and biochemical markers in HCV-infected Sindhi patients BJM
Riaz,Saba; Bashir,Muhammad Faisal; Haider,Saleem; Rahid,Naeem.
Abstract The presented study had two objectives. The first was to examine distributions of Hepatitis C Virus (HCV) genotypes in Sindh, Pakistan, where HCV is prevalent. The other was to explore clinically relevant relationships between the genotypes, viral load (measured by real-time polymerase chain reaction assays) and biochemical markers. For this, 1471 HCV-infected patients in six cities in Sindh were recruited and sampled. HCV genotype distributions varied among the cities, but genotype 3a was most prevalent, followed by 3b, 1a and 1b (detected in 51.5, 22.7. 9.25 and 3.2% of the cases, respectively). No type-specific sequences were detected in serum samples from 189 (12.8%) of the 1471 patients. Frequencies of low (<200,000 IU/mL serum),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sindh; HCV; Pakistan; Genotype; Biomarkers; Viral load.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400980
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Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles ArchiMer
Buffet, Pierre-emmanuel; Tankoua, Olivia Fossi; Pan, Jin-fen; Berhanu, Deborah; Herrenknecht, Christine; Poirier, Laurence; Amiard-triquet, Claude; Amiard, Jean-claude; Berard, Jean-baptiste; Risso, Christine; Guibbolini, Marielle; Romeo, Michele; Reip, Paul; Valsami-jones, Eugenia; Mouneyrac, Catherine.
Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 mu g L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of...
Tipo: Text Palavras-chave: Copper oxide nanoparticles; Behaviour; Biomarkers; Oxidative stress; Scrobicularia plana; Hediste diversicolor.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15140/15856.pdf
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Biochemical blood parameters: are they effective biomarkers of chronic toxicity? IPB - Escola Superior Agrária
Fernandes, Conceição; Fontaínhas-Fernandes, A.; Coimbra, A.; Saavedra, M.J.; Salgado, Maria Antónia.
The use of biomarkers has become attractive and useful for monitoring environmental quality and health of fish inhabiting polluted ecosystems. Among them, plasmatic biochemical parameters could be a promissory indicator of fish health condition. The question that arises is if these parameters can be applied to chronic toxicity. In this study we investigated the use of plasmatic biochemical parameters as indicators of fish chronic exposure to metals. Mullets (Liza saliens), inhabiting a metal polluted habitat, a small coastal lagoon Esmoriz-Paramos (Northwest coast, Portugal), showed alterations of plasmatic parameters related with Cu and Zn bioaccumulation in tissues. The effect of metals chronic exposure in plasmatic parameters is scarcely studied and...
Tipo: ConferenceObject Palavras-chave: Blood parameters; Biomarkers; Chronic toxicity.
Ano: 2009 URL: http://hdl.handle.net/10198/3843
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Biological effects of the "Erika" oil spill on the common mussel (Mytilus edulis) ArchiMer
Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois.
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during...
Tipo: Text Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf
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Biomarkers for screening of lung cancer and pre-neoplastic lesions in a high risk Chilean population Biol. Res.
Adonis,Marta I; Díaz,Jose; Miranda,Veronica R; Chahuan,Marco; Zambrano,Alcides; Benitez,Hugo C; Campos,Monica; Avaria,Pablo; Urzúa,Ulises; Marín,Pedro; Gohurdett,Mariela; Cisterna,Yasna; Gil,Lionel.
BACKGROUND: The mortality of lung cancer (LC), increases each year in the world, in spite of any advances, in development of new drugs to advance stages of LC. The high incidence of LC has been associated with smoking habit, genetic diversity and environmental pollution. Antofagasta region has been reported to have the highest LC mortality rate in Chile and its inhabitants were exposed to arsenic in their drinking water in concentrations as high as 870 &#956;g/L. Non-invasive techniques such as biomarkers (Automatic Quantitative Cytometry: AQC and DR70) and Auto Fluorescence Bronchoscopy (AFB) might be potentially useful as a supplementary diagnostic approach and early detection. Early detection is one of the most important factors to intervene and...
Tipo: Journal article Palavras-chave: Early detection; Biomarkers; Lung cancer.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100056
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Blood parameter analysis and morphological alterations as biomarkers on the health of Hoplias malabaricus and Geophagus brasiliensis BABT
Romão,Silvia; Donatti,Lucélia; Freitas,Matheus O.; Teixeira,Josiane; Kusma,Josiana.
This study aimed to assess the influence of the environment on fish health. Samples of Hoplias malabaricus and Geophagus brasiliensis, were collected from three different environments: area I was urban and areas II and III were rural. Analyses of red blood cell count, microhematocrit, hemoglobin concentration, white blood cell count and differential white cell count in blood smear were carried out. Mean corpuscular volume and mean corpuscular hemoglobin concentration were calculated. To analyze morphological alterations, gills, liver, kidney and gonads were submitted to routine histological processing. Individuals collected from area III had slightly lower blood indices than collected from area I . Severe kidney changes, degeneration of and crystallization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Bioindicators; Biomarkers; Morphological alterations; Hematology.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400012
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Branchial histopathological study of Brachyplatystoma rousseauxii (Castelnau, 1855) in the Guajará bay, Belém, Pará State, Brazil - DOI: 10.4025/actascibiolsci.v32i1.4800 Biological Sciences
Montes, Caroline da Silva; UFPR; Ferreira, Maria Auxiliadora Pantoja; UFPR; Santos, Simone do Socorro Damasceno; UFPR; Ledebur, Esther Iris C. Freifrau Von; UFPR; Rocha, Rossineide Martins; UFPR.
This work analyzes the morphological alterations in Brachyplatystoma rousseauxii gills and evaluates whether this species can be considered an environmental monitoring biomarker of Guajará Bay. Sampling was carried out in four areas around Belém, Brazil, in four annual periods: dry/wet season, wet season, wet/dry season and dry season. Water pH, temperature and suspended material were evaluated. A total of 36 specimens were collected. The second right gill arch of each animal was removed and immediately fixed and processed for histopathology analysis with light microscopy. The physicochemical analysis of the water during the study period showed slight acidity, temperature and material in suspension within normal levels. Histopathological analysis of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomarkers; Environmental monitoring; Histological alterations; Fish Morfologia biomarkers; Environmental monitoring; Histological alterations; Fish Histologia.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4800
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Cell culture from two red sea benthic invertebrates: a case study on the soft octocorals dendronephthya klunzingeri and anthelia glauca OceanDocs
Ammar, M.S.A.; Emara, A.M.; Perovic, S.; Wiens, M.; Billinghurst, Z.; Müller, I.M.; Müller, W.E.G..
Primmorphs were obtained from single cells of both coral species Dendronephthya klunzingeri and Anthelia glauca following incubation for three or four days during 2001. Both ultraviolet light (UVB; peak of emission of 320 nm) and visible light (wavelength between 400 and 520 nm with a maximum at 480 nm) were applied to the primmorphs as stressors. The following incubation probes have been isolated from D. klunzingeri and were used to monitor the expression of the respective genes in the homologous cell system; the heat-shock protein HSP90 (to monitor general stress responses), the histone H4cDNA (to monitor the cell cycle/proliferation) and the UVS-related protein (response to ultraviolet light) from D. klunzingeri. The data show that the steady state...
Tipo: Journal Contribution Palavras-chave: Biology Study; Biomarkers; Proteins; Proteins; Http://aims.fao.org/aos/agrovoc/c_6259.
Ano: 2007 URL: http://hdl.handle.net/1834/2218
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Characterization of acetylcholinesterase from the brain of the Amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides. Repositório Alice
ASSIS, C. R. D.; CASTRO, P. F. de; AMARAL, I. P. G.; CARVALHO, E. V. M. M.; CARVALHO JUNIOR, L. B.; BEZERRA, R. S..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Organophosphorus pesticide; Carbamate pesticide; Acetylcholinesterase; Biomarkers.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/871124
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Characterization of the aroma profile of novel Brazilian wines by solid-phase microextraction using polymeric ionic liquid sorbent coatings. Repositório Alice
CRUCELLO, J.; MIRON, L. F. O.; FERREIRA, V. H. C.; NAN, H.; MARQUES, M. O. M.; RITSCHEL, P. S.; ZANUS, M. C.; ANDERSON, J. L.; POPPI, R. J.; HANTAO, L. W..
In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from ?Isabella? and ?BRS Magna? cultivars?the latter was recently introduced by the Brazilian Agricultural Research Corporation ? National Grape & Wine Research Center. In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from ?Isabella? and ?BRS Magna? cultivars?the latter was recently introduced by the Brazilian Agricultural...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Flavor and aroma; Foodomics; Sensomics; Biomarkers; Food chemistry.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092008
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Chlamydia trachomatis and human papillomavirus coinfection: association with p16INK4a and Ki67 expression in biopsies of patients with pre-neoplastic and neoplastic lesions BJID
Calil,Luciane Noal; Igansi,Cristine Nascente; Meurer,Luise; Edelweiss,Maria Isabel Albano; Bozzetti,Mary Clarisse.
The objective of this study was to identify the frequency of coinfection by human papillomavirus (HPV) and Chlamydia trachomatis (CT) in cervical lesions and relate it with immunohistochemical expression of p16INK4a and Ki67, both oncogenicity markers. A cross-sectional study with 86 women from primary care units in southern Brazil was conducted. Cervical swabs were collected for HPV-DNA and CT-DNA detection, through the polymerase chain reaction technique (PCR). The immunohistochemical analysis was performed on biopsy cervical tissue material to identify the expression of p16INK4a and Ki67 cell cycle markers. About 83 % were positive for HPV-DNA and 19% had coinfection with CT-DNA. Among coinfected women, 56% expressed p16INK4a. There was a statistically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; Human papillomavirus 16; Biomarkers; Pharmacological.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200006
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Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer BJPS
Oliveira,Isadora Bernardo David de; Hirata,Rosario Dominguez Crespo.
Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may be valuable for early diagnosis and prognosis of CRC. The purpose of this review was to investigate the application of circulating cell-free DNA (ccfDNA) in CRC diagnosis and prognosis and the analytical methods used in blood samples in articles published between 2005 and 2016. Based on specific inclusion and exclusion criteria, 26 articles were selected. Most studies used ccfDNA quantification as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colorectal Neoplasms/diagnosis; Colorectal cancer; Circulating cell-free DNA; CcfDNA; Prognosis; Diagnosis; Biomarkers; Neoplastic Cells/circulating..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100406
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Clinical, biochemical and haemathological effects in Rhamdia quelen exposed to cypermethrin Arq. Bras. Med. Vet. Zootec.
Montanha,F.P.; Fredianelli,A.C.; Wagner,R.; Sacco,S.R.; Rocha,D.C.C.; Pimpão,C.T..
The acute intoxication of Cypermethrin in Silver Catfish (Rhamdia quelen) was evaluated. Animals weighing 56.67±4.43g and measuring 18.92±1.16cm were exposed to sublethal concentrations of Cypermethrin for the species in hydrological conditions during 96 hours. A total of 52 fish divided into three groups were used and received the following concentrations of Cypermethrin: 0 (n=12); 1.5 (n=20) and 2.5 (n=20)mg/L. The intoxicated animals suffered behavioral changes such as loss of balance, swimming alteration, dyspnea, upright swimming and sudden spiral swimming movements. As soon as the 96-hour period was over, a blood collection for hematological and biochemical analyses was performed. A complete haemogram test, plasmatic protein test, albumin, alanine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Biomarkers; Ecotoxicology; Haemogram; Pyrethroids.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300697
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