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Registros recuperados: 47
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A novel "Integrated Biomarker Response" calculation based on reference deviation concept ArchiMer
Sanchez, Wilfried; Burgeot, Thierry; Porcher, Jean-marc.
Multi-biomarker approaches are used to assess ecosystem health and identify impacts of environmental stress on organisms. However, exploration of large datasets by environmental managers represents a major challenge for regulatory application of this tool. Several integrative tools were developed to summarize biomarker responses. The aim of the present paper is to update calculation of the "Integrated Biological Response" (IBR) described by Beliaeff and Burgeot (Environ Toxicol Chem 21:1316-1322, 2002) to avoid weaknesses of this integrative tool. In the present paper, a novel index named "Integrated Biological Responses version 2" based on the reference deviation concept is presented. It allows a clear discrimination of sampling sites as for the IBR, but...
Tipo: Text Palavras-chave: Biomarkers; Data analysis; Graphic tools; Three-spined stickleback.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00139/25069/23191.pdf
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A pollution-monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha) ArchiMer
Minier, Christophe; Abarnou, Alain; Jaouen Madoulet, Agnes; Le Guellec, Anne-marie; Tutundjian, Renaud; Bocquene, Gilles; Leboulenger, François.
Zebra mussel (Dreissena polymorpha) is an invasive species that has proliferated in European and North American rivers and lakes during the last century. In this study, D. polymorpha has been used to provide information on contamination levels and biological effects in the Seine Estuary (France). The bivalves accumulated polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) to a high degree with values reaching 800 ng/g dry weight for PCBs (sum of 20 congeners), and 1,000 ng/g dry weight of PAHs (sum of 14 compounds) in the whole body. These values are among the highest reported of PCBs and, to a lesser extent, of PAHs in other contaminated areas in the world. Toxic equivalent quantities of PCBs and PAHs detected in zebra mussels...
Tipo: Text Palavras-chave: Biomarkers; Multixenobiotic resistance; Polycyclic aromatic hydrocarbons; Polychlorinated biphenyls; Dreissena polymorpha.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2130.pdf
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Active biomonitoring in Greek coastal waters: Application of the integrated biomarker response index in relation to contaminant levels in caged mussels ArchiMer
Tsangaris, Catherine; Hatzianestis, Ioannis; Catsiki, Vassiliki-angelique; Kormas, Konstantinos Ar; Strogyloudi, Evangelia; Neofitou, Christos; Andral, Bruno; Galgani, Francois.
An integrated approach using biomarkers and contaminant levels in mussels Mytilus galloprovincialis L was employed to assess chemical contamination in Greek coastal waters within the framework of the MYTIMED program. Biomarkers (metallothioneins, glutathione S-transferase, catalase, acetylcholinesterase and RNA:DNA ratio) have been previously described in mussels caged at 14 sites in coastal areas influenced by different types of anthropogenic activities. This study applied a biomarker index, the Integrated Biomarker Response (IBR) to summarize biomarker responses and relate stress levels to concentrations of organochlorine compounds (PCBs, DDTs), polycyclic aromatic hydrocarbons (PAHs), and metals (Cu, Ni, Fe, Zn) measured in the mussel tissues. The IBR...
Tipo: Text Palavras-chave: Pollution; Biomarkers; Monitoring; Mussel caging; Mytilus galloprovincialis; Mediterranean Sea.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00059/17033/14718.pdf
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Acute exposure of fish to nano-TiO2 at environmental levels of ultraviolet light. Repositório Alice
CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Dióxido de titânio; Nano-TiO2; Nanotecnologia; Substância tóxica; Impacto ambiental; Peixe; Pacu; Biomarkers; Ecotoxicology; Fish; Nanotechnology; Piaractus mesopotamicus; Titanium dioxide; Ultraviolet radiation.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/981722
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Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil ArchiMer
Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy.
Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)...
Tipo: Text Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45828/45916.pdf
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Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles ArchiMer
Buffet, Pierre-emmanuel; Tankoua, Olivia Fossi; Pan, Jin-fen; Berhanu, Deborah; Herrenknecht, Christine; Poirier, Laurence; Amiard-triquet, Claude; Amiard, Jean-claude; Berard, Jean-baptiste; Risso, Christine; Guibbolini, Marielle; Romeo, Michele; Reip, Paul; Valsami-jones, Eugenia; Mouneyrac, Catherine.
Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 mu g L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of...
Tipo: Text Palavras-chave: Copper oxide nanoparticles; Behaviour; Biomarkers; Oxidative stress; Scrobicularia plana; Hediste diversicolor.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15140/15856.pdf
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Biochemical blood parameters: are they effective biomarkers of chronic toxicity? IPB - Escola Superior Agrária
Fernandes, Conceição; Fontaínhas-Fernandes, A.; Coimbra, A.; Saavedra, M.J.; Salgado, Maria Antónia.
The use of biomarkers has become attractive and useful for monitoring environmental quality and health of fish inhabiting polluted ecosystems. Among them, plasmatic biochemical parameters could be a promissory indicator of fish health condition. The question that arises is if these parameters can be applied to chronic toxicity. In this study we investigated the use of plasmatic biochemical parameters as indicators of fish chronic exposure to metals. Mullets (Liza saliens), inhabiting a metal polluted habitat, a small coastal lagoon Esmoriz-Paramos (Northwest coast, Portugal), showed alterations of plasmatic parameters related with Cu and Zn bioaccumulation in tissues. The effect of metals chronic exposure in plasmatic parameters is scarcely studied and...
Tipo: ConferenceObject Palavras-chave: Blood parameters; Biomarkers; Chronic toxicity.
Ano: 2009 URL: http://hdl.handle.net/10198/3843
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Biological effects of the "Erika" oil spill on the common mussel (Mytilus edulis) ArchiMer
Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois.
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during...
Tipo: Text Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf
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Biomarkers for screening of lung cancer and pre-neoplastic lesions in a high risk Chilean population Biol. Res.
Adonis,Marta I; Díaz,Jose; Miranda,Veronica R; Chahuan,Marco; Zambrano,Alcides; Benitez,Hugo C; Campos,Monica; Avaria,Pablo; Urzúa,Ulises; Marín,Pedro; Gohurdett,Mariela; Cisterna,Yasna; Gil,Lionel.
BACKGROUND: The mortality of lung cancer (LC), increases each year in the world, in spite of any advances, in development of new drugs to advance stages of LC. The high incidence of LC has been associated with smoking habit, genetic diversity and environmental pollution. Antofagasta region has been reported to have the highest LC mortality rate in Chile and its inhabitants were exposed to arsenic in their drinking water in concentrations as high as 870 μg/L. Non-invasive techniques such as biomarkers (Automatic Quantitative Cytometry: AQC and DR70) and Auto Fluorescence Bronchoscopy (AFB) might be potentially useful as a supplementary diagnostic approach and early detection. Early detection is one of the most important factors to intervene and...
Tipo: Journal article Palavras-chave: Early detection; Biomarkers; Lung cancer.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100056
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Branchial histopathological study of Brachyplatystoma rousseauxii (Castelnau, 1855) in the Guajará bay, Belém, Pará State, Brazil - DOI: 10.4025/actascibiolsci.v32i1.4800 Biological Sciences
Montes, Caroline da Silva; UFPR; Ferreira, Maria Auxiliadora Pantoja; UFPR; Santos, Simone do Socorro Damasceno; UFPR; Ledebur, Esther Iris C. Freifrau Von; UFPR; Rocha, Rossineide Martins; UFPR.
This work analyzes the morphological alterations in Brachyplatystoma rousseauxii gills and evaluates whether this species can be considered an environmental monitoring biomarker of Guajará Bay. Sampling was carried out in four areas around Belém, Brazil, in four annual periods: dry/wet season, wet season, wet/dry season and dry season. Water pH, temperature and suspended material were evaluated. A total of 36 specimens were collected. The second right gill arch of each animal was removed and immediately fixed and processed for histopathology analysis with light microscopy. The physicochemical analysis of the water during the study period showed slight acidity, temperature and material in suspension within normal levels. Histopathological analysis of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomarkers; Environmental monitoring; Histological alterations; Fish Morfologia biomarkers; Environmental monitoring; Histological alterations; Fish Histologia.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4800
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Cell culture from two red sea benthic invertebrates: a case study on the soft octocorals dendronephthya klunzingeri and anthelia glauca OceanDocs
Ammar, M.S.A.; Emara, A.M.; Perovic, S.; Wiens, M.; Billinghurst, Z.; Müller, I.M.; Müller, W.E.G..
Primmorphs were obtained from single cells of both coral species Dendronephthya klunzingeri and Anthelia glauca following incubation for three or four days during 2001. Both ultraviolet light (UVB; peak of emission of 320 nm) and visible light (wavelength between 400 and 520 nm with a maximum at 480 nm) were applied to the primmorphs as stressors. The following incubation probes have been isolated from D. klunzingeri and were used to monitor the expression of the respective genes in the homologous cell system; the heat-shock protein HSP90 (to monitor general stress responses), the histone H4cDNA (to monitor the cell cycle/proliferation) and the UVS-related protein (response to ultraviolet light) from D. klunzingeri. The data show that the steady state...
Tipo: Journal Contribution Palavras-chave: Biology Study; Biomarkers; Proteins; Proteins; Http://aims.fao.org/aos/agrovoc/c_6259.
Ano: 2007 URL: http://hdl.handle.net/1834/2218
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Characterization of acetylcholinesterase from the brain of the Amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides. Repositório Alice
ASSIS, C. R. D.; CASTRO, P. F. de; AMARAL, I. P. G.; CARVALHO, E. V. M. M.; CARVALHO JUNIOR, L. B.; BEZERRA, R. S..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Organophosphorus pesticide; Carbamate pesticide; Acetylcholinesterase; Biomarkers.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/871124
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Characterization of the aroma profile of novel Brazilian wines by solid-phase microextraction using polymeric ionic liquid sorbent coatings. Repositório Alice
CRUCELLO, J.; MIRON, L. F. O.; FERREIRA, V. H. C.; NAN, H.; MARQUES, M. O. M.; RITSCHEL, P. S.; ZANUS, M. C.; ANDERSON, J. L.; POPPI, R. J.; HANTAO, L. W..
In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from ?Isabella? and ?BRS Magna? cultivars?the latter was recently introduced by the Brazilian Agricultural Research Corporation ? National Grape & Wine Research Center. In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from ?Isabella? and ?BRS Magna? cultivars?the latter was recently introduced by the Brazilian Agricultural...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Flavor and aroma; Foodomics; Sensomics; Biomarkers; Food chemistry.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092008
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Cultured Pearl Surface Quality Profiling by the Shell Matrix Protein Gene Expression in the Biomineralised Pearl Sac Tissue of Pinctada margaritifera ArchiMer
Blay, Carole; Planes, Serge; Ky, Chin-long.
Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis. The pearls and pearl sacs analysed were from a uniform experimental graft with sequential...
Tipo: Text Palavras-chave: Biomineralisation; Relative gene expression; Pearl sac; Biomarkers; Pearl quality; Pinctada margaritifera.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00435/54622/56022.pdf
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Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata ArchiMer
Auguste, M.; Mestre, N. C.; Rocha, T. L.; Cardoso, C.; Cueff-gauchard, Valerie; Le Bloa, Simon; Cambon-bonavita, Marie-anne; Shillito, B.; Zbinden, M.; Ravaux, J.; Bebianno, M. J..
In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30 MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630 m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues...
Tipo: Text Palavras-chave: Deep-sea mining; Hydrothermal vents; Biomarkers; Ipocamp; Copper; TAG.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00323/43465/42869.pdf
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Diatomeas antárticas: vida en las sombras y en el frío OceanDocs
Uribe, P..
En la Antártica se ha descrito un grupo de microalgas (diatomeas bentónicas), que viven en el fondo, pegadas a las piedras o sedimentos, capaces de hacer fotosíntesis con niveles de luz muy bajos e incluso cubiertas por una capa de hielo marino. El proyecto “Identificación de biomarcadores de fluctuaciones de la cobertura de hielo en diatomeas bentónicas de la Península Antártica” busca conocer las características fisiológicas que determinan la adaptación de estas microalgas al medio polar, características que luego sirvan como marcadores biológicos (o biomarcadores) de esta capacidad de vivir en las zonas más oscuras. Paulina Uribe describe este proyecto y establece un curioso vínculo con las ideas y circunstancias de Darwin.
Tipo: Journal Contribution Palavras-chave: Algae; Biomarkers; Benthos; Benthos; Algae; Http://aims.fao.org/aos/agrovoc/c_878; Http://aims.fao.org/aos/agrovoc/c_258.
Ano: 2009 URL: http://hdl.handle.net/1834/3366
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Distribution of the organic matter in the channel-levees systems of the Congo mud-rich deep-sea fan (West Africa). Implication for deep offshore petroleum source rocks and global carbon cycle ArchiMer
Baudin, Francois; Disnar, Jean-robert; Martinez, Philippe; Dennielou, Bernard.
The quantity and the source of organic matter preserved in the Recent turbiditic channel-levees systems around 4000 m-depth off the Congo River were determined using bulk geochemical approaches (Rock-Eval, elemental and isotopic analyses) as well as molecular and optical analyses on selected samples. These mud-rich sediments contain high amount of organic matter (3% Corg on average), the origin of which is a mixture of terrestrial higher-plant debris and deeply oxidized phytoplanktonic material. Although the relative contribution of continental source versus marine source of the organic matter cannot be precisely quantified, the continental fraction appears significant (at least 70-80%) especially for such depths and distances from the coast. The organic...
Tipo: Text Palavras-chave: South-East Atlantic; Turbidites; Carbon isotopes; Nitrogen isotopes; Biomarkers; Source rocks.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11700/9285.pdf
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Effects of increasing temperatures on biomarker responses and accumulation of hazardous substances in rope mussels (Mytilus galloprovincialis) from Bizerte lagoon ArchiMer
Kamel, Naouel; Burgeot, Thierry; Banni, Mohamed; Chalghaf, Mohamed; Devin, Simon; Minier, Christophe; Boussetta, Hamadi.
This study examined the influence of increasing temperatures in spring and summer on biochemical biomarkers in Mytilus galloprovincialis mussels sampled from Bizerte lagoon (northern Tunisia). Spatial and seasonal variations in a battery of seven biomarkers were analyzed in relation to environmental parameters (temperature, salinity and pH), physiological status (condition and gonad indexes), stress on stress (SoS) and chemical contaminant levels (heavy metals, PAHs and PCBs) in digestive glands. Integrated Biological Response (IBR) was calculated using seven biomarkers (AChE, BPH, MXR, GST, CAT, MDA and MT). Seasonal variations in biological response were determined during a critical period between spring and summer at two sites, where chemical...
Tipo: Text Palavras-chave: Biomarkers; Mussels; Lagoon biomonitoring; Integrated biomarker response.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28224/26471.pdf
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Effects of water accommodated fractions of crude oils and diesel on a suite of biomarkers in Atlantic cod (Gadus morhua) ArchiMer
Holth, T. F.; Eidsvoll, D. P.; Farmen, E.; Sanders, M. B.; Martinez-gomez, C.; Budzinski, H.; Burgeot, Thierry; Guilhermino, L.; Hylland, K..
The aim of this study was to characterise concentration- and time-dependent responses in juvenile Atlantic cod (Gadus morhua) following exposure for one and three weeks to the water-soluble fraction (WAF) of three weathered oils: Arabian Light crude oil (ALC), North Sea crude oil (NSC) and ship-diesel. The sum of polycyclic aromatic hydrocarbons (PAH) in water was highest after one week of exposure and within environmentally relevant concentrations. PAH metabolites in bile confirmed exposure to and uptake of PAHs. Hepatic cytochrome P450 1A (CYP1A) gene expression (here and later the phrase gene expression is used synonymously to gene transcription, although it is acknowledged that gene expression is also regulated by, e.g., translation and protein...
Tipo: Text Palavras-chave: Atlantic cod; Biomarkers; Weathered crude oil; Ship-diesel; Water accommodated fraction; Time-course.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00190/30131/28593.pdf
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Environmental stress in the pacific oyster Crassostrea gigas during gametogenesis : role of herbicides? ArchiMer
Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F..
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was...
Tipo: Text Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf
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