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Registros recuperados: 8
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Amperometric biosensor for Salmonella typhimurium detection in milk. Repositório Alice
ALEXANDRE, D. L.; MELO, A. M. A.; FURTADO, R. F.; BORGES, M. de F.; FIGUEIREDO, E. A. T.; BISWAS, A.; CHENG, H. N.; ALVES, C. R..
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Salmonella; Biosensor; Electrochemical; Milk; Salmonela; Biossensor; Eletroquímico; Leite.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1059862
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Construction and application of a built-in dual luciferase reporter for microRNA functional analysis Electron. J. Biotechnol.
Bi,Yanzhen; Zheng,Xinmin; Shao,Changwei; Pan,Wen; Jiang,Li; Ouyang,Huiwu.
Background: As key gene regulators, microRNAs post-transcriptionally modulate gene expression via binding to partially complementary sequence in the 3? UTR of target mRNA. An accurate, rapid and quantitative tool for sensing and validation of miRNA targets is of crucial significance to decipher the functional implications of miRNAs in cellular pathways. Results: Taking advantage of an improved restriction-free cloning method, we engineered a novel built-in dual luciferase reporter plasmid where Firefly and Renilla luciferase genes were assembled in a single plasmid named ?pFila?. This design eliminates the transfection of a separate control plasmid and thus minimizes the time and labor required for miRNA-target sensing assays. pFila consistently produces...
Tipo: Journal article Palavras-chave: Biosensor; Luciferase; Ligase-independent; MiRNA; Target.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200011
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Efeito in vitro de poluentes inorgânicos usados na agricultura sobre a atividade da catalase da alga Pseudokirchneriella subcapitata. Repositório Alice
DUARTE, S.; AMARAL SOBRINHO, N. M. B.; JONSSON, C. M.; JOKL, L..
Resumo: O efeito de poluentes em ecossistemas pode ser verifi cado pela sua ação sobre os produtores primários, como a alga Pseudokirchneriella subcapitata (Korshikov) F. Hindák (Chlorophyceae), que tem ampla distribuição em águas dulcícolas e no solo. O efeito de oito íons metálicos empregados na agricultura (alumínio, cádmio, chumbo, magnésio, manganês, mercúrio, selênio e zinco) sobre a atividade enzimática em P. subcapitata foi estudado, usando a catalase como marcador bioquímico. Alumínio, chumbo, mercúrio e zinco foram considerados inibidores e magnésio e manganês como ativadores da ação enzimática. A atividade da catalase na presença de cádmio e selênio não foi alterada. A atividade da catalase pode ser uma ferramenta promissora como biossensor...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biosensor; Íons metálicos; Atividade enzimática; Pseudokirchneriella; Alga; Poluente; Impacto ambiental; Metal pesado; Algae; Biosensors; Catalase; Pollutants; Environmental impact.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1009712
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In situ QCM DNA-biosensor probe modification ArchiMer
Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.
We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA...
Tipo: Text Palavras-chave: QCM; DNA; Biosensor.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf
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Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology ArchiMer
Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Thioune, Nene; David, Carmen; De La Chapelle, Marc; Compere, Chantal.
We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with...
Tipo: Text Palavras-chave: Biosensor; Surface-enhanced Raman scattering; Polycyclic aromatic hydrocarbons; Benzo[a]pyrene antibody; Organometallic nanoprobe.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00417/52844/53870.pdf
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Strategy to design DNA-biosensors: Single-stranded probe grafting versus target-probe duplex grafting ArchiMer
Vedrine, C.; Lazerges, M.; Perrot, H.; Compere, Chantal; Pernelle, C..
A strategy to design DNA-biosensors by grafting probe-target duplex onto a 27 MHz quartz crystal microbalance gold surface is presented in this work. The idea that removing the DNA target after duplex grafting yields to a well accessible probe, and therefore to efficient target recognition, is investigated. Unfortunately, hybridization efficiency measured using such a straightaway protocol is equal to 15%, which is feeble by comparison with the 31% hybridization efficiency measured for biosensors designed with single-stranded probe. Successive DNA biosensors are designed in this work, taking into account phenomena that occur specifically in an interfacial environment, like non specific adsorption or exchange reactions between adsorbed molecules. A...
Tipo: Text Palavras-chave: Alexandrium minutum; Biosensor; Double-stranded DNA; Quartz crystal microbalance.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21196/19416.pdf
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Surface plasmon resonance in chalcogenide glass-based optical system ArchiMer
Le Person, Jenny; Colas, Florent; Compere, Chantal; Lehaitre, Michel; Anne, M; Boussard Pledel, C; Bureau, B; Adam, J; Deputier, S; Guilloux Viry, M.
The surface plasmon resonance phenomenon has been studied in a chalcogenide glass-based optical system. IR transmission properties of these materials combined to their high refractive indices lead to advantageous properties for sensing. In this study, numerical simulations have been carried out to investigate the potentialities of sulfide glass from the GeGaSbS system as a coupling prism material. Then, an angular modulation SPR biosensor has been set up in the Kretschmann-Raether arrangement. Experimental data are consistent with numerical calculation and the detection limit of the sensor is 3 x 10(-5) RIU. These preliminary results are promising. Further investigations have to be carried out to confirm the great potentialities of those materials for...
Tipo: Text Palavras-chave: Infrared; Biosensor; Surface plasmon resonance; Chalcogenide glass.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3955.pdf
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Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal.
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter...
Tipo: Text Palavras-chave: Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21810/19385.pdf
Registros recuperados: 8
Primeira ... 1 ... Última
 

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