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Registros recuperados: 9
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A gravity model approach to forecasting tuberculosis transmission in cattle AgEcon
Xie, Fang; Horan, Richard D.; Wolf, Christopher A..
Bovine tuberculosis (bTB) in cattle has caused significant economic losses to livestock producers and has proven difficult to eradicate. It is suspected that cattle movement across different farms and regions is one of the key factors of bTB transmission in the United States. Prior attempts to model the epidemiology of bTB infection within cattle to predict disease transmission have not adequately captured the behavioral aspects of trade. A better understanding of livestock trade patterns would help in predicting disease transmission and the associated economic effects. In this paper, we develop a gravity model of livestock trade and link it to an epidemiological model of bTB transmission, with the goal being that this information could lead to improved...
Tipo: Conference Paper or Presentation Palavras-chave: Bovine tuberculosis; Gravity model; Disease management; Resource /Energy Economics and Policy.
Ano: 2009 URL: http://purl.umn.edu/49382
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Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR Ciência Rural
Moura,André; Hodon,Mikael Arrais; Soares Filho,Paulo Martins; Issa,Marina de Azevedo; Oliveira,Ana Paula Ferreira de; Fonseca Júnior,Antônio Augusto.
ABSTRACT: Bovine tuberculosis is an infectious disease with a high impact on the cattle industry, particularly in developing countries. PCR is a very sensitive method for detection of infectious agents, but the sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods. The objective of this study was to evaluate DNA extraction methods for direct detection of Mycobacterium bovis in bovine tissue. Nine commercial kits for DNA extraction were evaluated when combined with two real time PCRs. The DNeasy Blood & Tissue Kit from QIAGEN showed better performance and sensitivity followed by the DNA Mini Kit RBC and FTA Elute Micro Card. Results suggested that, even when the analytical sensitivity of the qPCR is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; DNA extraction; Real time PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223
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Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR BJM
Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
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Epidemiology of bovine tuberculosis in Mexico, bordering the United States, at establishment of controlling strategies Arq. Bras. Med. Vet. Zootec.
Enríquez-Cruz,C.; Cruz-Hernández,N.I.; Zertuche-Rodríguez,J.L.; Uriegas-García,J.L.; Toscano-Ruiz,J.E.; Flores-Gutiérrez,G.H..
A case-control study was carried out for determining epidemiological patterns at the beginning of the Mexican campaign against bovine tuberculosis (BTB) in the State of Tamaulipas, Mexico, bordering the United States of America. From January 1995 to March 1998, 599 direct tissue smears of cattle from routine slaughterhouse inspection were analyzed for the presence of Mycobacterium bovis acid-fastness with Ziehl-Neelsen stain. BTB status was associated with year of diagnosis, age, breed, geographical origin of cattle, and type of slaughterhouse using the odds ratio (OR) (95% confidence level). The prevalence at slaughterhouse surveillance was 15.36%, which was considerably higher in 1995 (41.38%, OR=5.78, P<0.0001), in comparison with 8.09%, 14.42%, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; United States/Mexico border; Epidemiologic control.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500002
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Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain BJMBR
Alito,A.; McNair,J.; Girvin,R.M.; Zumarraga,M.; Bigi,F.; Pollock,J.M.; Cataldi,A..
Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulating antigens. These fractions were incubated with peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle in lymphoproliferation assays. This procedure does not denature proteins and permits the testing of mixtures of potential antigens that could be later identified. We characterized protein fractions with high stimulation indices from both culture supernatants and cell extracts....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Antigens; Mycobacterium bovis; Cellular immunity.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100011
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Identification of Mycobacterium bovis Isolates by a multiplex PCR BJM
Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004
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Molecular typing of Mycobacterium bovis isolated in the south of Brazil BJM
Ramos,Daniela Fernandes; Silva,Ana Bárbara Scholante; Fagundes,Michel Quevedo; von Groll,Andrea; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio.
Bovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the frequent movement of cattle between different properties.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Mycobacterium bovis; Genotyping.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200039
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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex BJM
Spositto,F.L.E.; Campanerut,P.A.Z.; Ghiraldi,L.D.; Leite,C.Q.F.; Hirata,M.H.; Hirata,R.D.C.; Siqueira,V.L.D.; Cardoso,R. Fressatti.
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Tuberculosis; PCR-multiplex; Diagnosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012
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Prevalence of bovine tuberculosis in the Aksu Region of Xinjiang, China, between 1985 and 2016 Arq. Bras. Med. Vet. Zootec.
Liu,Y.H.; Kang,Q.; Yang,B.; Li,F.; Li,X.Q.; Zhang,L.; Zhao,L..
ABSTRACT Prevalence of bovine tuberculosis infection in cattle in Aksu Prefecture determined by intradermal tuberculin skin test (TST), between 1985 and 2016. Cattle were analyzed according to region, feeding pattern, herds and age. A total of 890,009 cattle were tested, with overall bovine tuberculosis prevalence of 0.13% (1172/890009). Statistically significant difference was found in feeding pattern and herds. Prevalence in cows (0.19%, 615/327022) was higher than that in beeves (P< 0.01, OR= 1.903, 95% CI = 1.696 to 2.134). Significant difference (P< 0.01; OR= 2.238, 95%; CI= 1.937 to 2.585) was evident for rates for bovine tuberculosis in the peasant household (0.12%, 942/802343) and farm groups (0.26%, 230/87666). The overall prevalence of bTB...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prevalence; Bovine tuberculosis; Aksu prefecture.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200374
Registros recuperados: 9
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