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Registros recuperados: 7
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Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis BJPS
Cârcu-Dobrin,Melania; Sabău,Alexandra Georgiana; Hancu,Gabriel; Árpád,Gyéresi; Rusu,Aura; Kelemen,Hajnal; Papp,Lajos Attila; Cârje,Anca.
The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 ºC, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amlodipine; Capillary electrophoresis; Chiral separation; Cyclodextrine; Experimental design.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100530
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Comparison of capillary electrophoresis and high performance liquid chromatography methods for caffeine determination in decaffeinated coffee Ciênc. Tecnol. Aliment.
Bizzotto,Carolina Schaper; Meinhart,Adriana Dillenburg; Ballus,Cristiano Augusto; Ghiselli,Gislaine; Godoy,Helena Teixeira.
Decaffeinated coffee accounts for 10 percent of coffee sales in the world; it is preferred by consumers that do not wish or are sensitive to caffeine effects. This article presents an analytical comparison of capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) methods for residual caffeine quantification in decaffeinated coffee in terms of validation parameters, costs, analysis time, composition and treatment of the residues generated, and caffeine quantification in 20 commercial samples. Both methods showed suitable validation parameters. Caffeine content did not differ statistically in the two different methods of analysis. The main advantage of the high performance liquid chromatography (HPLC) method was the 42-fold lower...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caffeine; Capillary electrophoresis; HPLC; Method comparison; Decaffeinated coffee.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100027
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Determinação potenciométrica em fluxo de cloreto de cetilpiridinio em desinfectantes bucais IPB - Escola Superior Agrária
Baptista, Paula; Araújo, Alberto N.; Montenegro, Maria da Conceição B. S. M..
The work describes a new procedure for cetylpyridinium chloride determination in oral disinfectants, based on a flow-injection system with potentiometric detection. The determination was based on the measurement of picrate concentration decrease as result of ion-pair reaction with the analyte present in the injected sample. In the optimised set-up the sample injection volume was kept at 400 µL and merged downstream with the reagent solution containing 1,0 x10-5 mol/L of picrate adjusted to pH 5.0 with citrate/citric acid buffer. The flow rate was fixed at 8 mL/min and the reactor length at 40 cm. The proposed procedure enables the determination of cetylpyridinium in the analytical range of 5,0x10-6 – 7,5x10-5 mol/L at a sampling rate of 60/h. The results...
Tipo: Article Palavras-chave: Potentiometry; Oral disinfectants; FIA; Ion-selective electrodes; Cationic surfactants; Spectrophotometric determination; Pharmaceutical preparations; Capillary electrophoresis; Pair extraction; Construction; Titrations; Sensor.
Ano: 2003 URL: http://hdl.handle.net/10198/938
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Development of a generic method for the determination of proton-pump inhibitors by capillary zoneelectrophoresis BJPS
Papp,Lajos Attila; Gyéresi,Árpád; Hancu,Gabriel; Mircia,Eleonora; Kelemen,Hajnal.
A generic capillary zone electrophoresis method was developed for the analysis of four proton pump inhibitors: omeprazole, pantoprazole, lansoprazole and rabeprazole. During preliminary analysis screening of phosphate buffers at different pH levels was performed, in order to determine the optimum pH domain suitable for the simultaneous determination of all studied compounds. A face centered central composite design was employed for the optimization of separation conditions. The effect of buffer concentration, pH and applied voltage was studied; resolution between peaks and migration time of the last compound were considered as responses. Other factors as system temperature, injection parameters, capillary length, were held constant during the optimization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capillary electrophoresis; Proton-pump inhibitors; Experimental design.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100534
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Development of a method for simultaneous analysis of caffeine and taurine in energy drinks by micellar electrokinetic chromatography with diode-array detector Ciênc. Tecnol. Aliment.
ALVES,Alane Cangani; MEINHART,Adriana Dillenburg; TEIXEIRA FILHO,José; GODOY,Helena Teixeira.
Abstract The objective of this study was to develop, optimize and validate a fast and reliable method for the simultaneous determination of caffeine and taurine contents by micellar electrokinetic chromatography with diode array detector, using direct and indirect detection concomitantly. Multivariate statistical techniques were used as a central composite design and the simultaneous optimization method of responses of Derringer and Suich were used for optimization. The method was applied in the analysis of 73 samples of energy drinks commercialized in Brazil. The optimized method employed a capillary tube with an extended bulb of 50 µm i.d. x 72 cm total length, an electrolyte containing 16.20 mmol.L-1 of benzoic acid and 39.90 mmol.L-1 of SDS, a pH value...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multivariate optimization; Added ingredients; Capillary electrophoresis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600673
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Enantioselective metabolism of hydroxychloroquine employing rats and mice hepatic microsomes BJPS
Cardoso,Carmem Dickow; Bonato,Pierina Sueli.
Hydroxychloroquine (HCQ) is an important chiral drug used, mainly, in the treatment of rheumatoid arthritis, systemic lupus erythematosus and malaria, and whose pharmacokinetic and pharmacodynamic properties look to be stereoselective. Respecting the pharmacokinetic properties, some previous studies indicate that the stereoselectivity could express itself in the processes of metabolism, distribution and excretion and that the stereoselective metabolism looks to be a function of the studied species. So, the in vitro metabolism of HCQ was investigated using hepatic microsomes of rats and mice. The microsomal fraction of livers of Wistar rats and Balb-C mice was separated by ultracentrifugation and 500 μL were incubated for 180 minutes with 10 μL of racemic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydroxychloroquine; Stereoselective metabolism/in vitro study; High performance liquid chromatography; Capillary electrophoresis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400008
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Genetic diversity and adaptability of two species of Mugilidae (Teleostei : Perciformes) of the Po river delta coastal lagoons ArchiMer
Papa, R; Nonnis Marzano, F; Rossi, V; Gandolfi, G.
Molecular polymorphisms (allozyme and amplified fragment length polymorphisms (AFLP)) were investigated in the mullet specie's Liza ramada and Liza saliens to determine levels of genetic variability and assess possible correlations between the population genetic structure and the specie's adaptability to shallow water environments. The thinlipped (L. ramada) and the sharpnose (L saliens) mullets are diadromous species-that enter coastal lagoons during early life stages to complete growth previous to sexual maturation. Bi-monthly samples (n = 30-50) were collected in the period 2000-2002 in different sites of the Sacca di Goro (Po river delta) and a nearby aquaculture lagoon (Valle Bertuzzi). Biochemical analyses were carried out by means of starch gel...
Tipo: Text Palavras-chave: AFLP; Systèmes enzymatiques; Electrophorèse capillaire; Liza spp; Polymorphisme moléculaire; AFLP; Allozyme; Capillary electrophoresis; Liza spp.; Molecular polymorphisms.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43292/43027.pdf
Registros recuperados: 7
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