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Caffeine formation by suspension cultures of Coffea dewevrei BABT
Sartor,Rosana Mary; Mazzafera,Paulo.
The low caffeine content in leaves of C. dewevrei (~ 0.5 mg/g) is due to a low biosynthesis associated with a fast degradation. On the other hand, high biosynthesis and low degradation confer a higher content (~ 8 mg/g) in leaves of C. arabica. In this work it was observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with labelled carbon dioxide showed a significant accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves, and therefore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Caffeine; Cell suspension culture; Methylxanthines.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100009
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Detection of anthraquinones and identification of 1,4-naphthohydroquinone in cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae) Rev. Bras. Bot.
Oliveira,Marisa de Cacia; Negri,Giusepina; Salatino,Antônio; Braga,Márcia Regina.
In Rubiaceae, anthraquinones and naphthoquinones are secondary metabolites characteristic of the subfamily Rubioideae, in which Rudgea jasminoides is included. Thin-layer chromatography using specific solvent systems and spray reagents indicated the presence of anthraquinones constitutively produced by cell suspension cultures of R. jasminoides. GC/MS analysis detected 1,4-naphthohydroquinone as a product of biosynthesis only after elicitation of the cells with yeast extract (Saccharomyces cerevisiae). The latter compound is probably a phytoalexin produced by suspension cultures of R. jasminoides.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell suspension culture; Elicitor; Naphtoquinones; Rubiaceae.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042007000100017
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Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum PAB
KOBAYASHI,ADILSON KENJI; VIEIRA,LUIZ GONZAGA ESTEVES.
An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mug/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Bacterial blight; Extracellular polysaccharides; Cell suspension culture; Hypersensitive response.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400007
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Establishment of cell suspension and protoplast culture of a wild rice species (Oryza ridleyi Hook F.) International Rice Research Institute
Batalla-Villegas, Maria Lourdes.
74 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Wild relatives; Oryza ridleyi; Tissue culture; Cell suspension culture; Protoplasts; Regeneration.
Ano: 1989 URL: http://hdl.handle.net/123456789/1173
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In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae) Acta Botanica
Balbuena,Tiago Santana; Santa-Catarina,Claudete; Silveira,Vanildo; Kato,Massuo Jorge; Floh,Eny Iochevet Segal.
Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Cell suspension culture; Morphogenesis; Pariparoba; Plant growth regulators.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100029
Registros recuperados: 5
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