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Registros recuperados: 11
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Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria Electron. J. Biotechnol.
Odisi,Estácio Jussie; Silvestrin,Marcela Bruschi; Takahashi,Rodrigo Yoji Uwamori; da Silva,Marcus Adonai Castro; Lima,André Oliveira de Souza.
Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at...
Tipo: Journal article Palavras-chave: Bacteria; Cellulases; Deep-sea; Lipases.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018
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Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium Electron. J. Biotechnol.
Quiroz-Castañeda,Rosa E; Balcázar-López,Edgar; Dantán-González,Edgar; Martinez,Alfredo; Folch-Mallol,Jorge; Martínez Anaya,Claudia.
Cellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic...
Tipo: Journal article Palavras-chave: B. adusta; Cellulases; P. sanguineus; Wheat straw; Xylanases.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400005
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Cloning and expression of cellulase XF-818 of Xylella fastidiosa in Escherichia Coli Scientia Agricola
Wulff,Nelson Arno; Carrer,Helaine; Pascholati,Sérgio Florentino.
Xylella fastidiosa's genome was the first of a plant pathogen to be completely sequenced. Through comparative sequence analysis many genes were identified and, among them, several potentially involved in plant-pathogen interaction. However, the biological role of each gene should be assigned experimentally. On this regard, heterologous protein expression is a powerful tool to produce proteins from such genes, allowing their characterization. X. fastidiosa lives inside xylem vessels and eventually would degrade pit membranes from xylem cells to move radialy into the host. The identification of several putative plant cell wall degrading enzymes on X. fastidiosa genome prompted the assession of the function of such proteins. The open reading frame (ORF)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus variegated chlorosis; Cellulases; Cloning and expresssion.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400016
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Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1 BJM
Gottschalk,Leda Maria Fortes; Paredes,Raquel de Sousa; Teixeira,Ricardo Sposina Sobral; Silva,Ayla Sant'Ana da; Bon,Elba Pinto da Silva.
The production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, β-xylosidase and ferulic acid esterase to a peak concentrations of 44,880; 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus awamori 2B.361 U2/1; Cellulases; Hemicellulases; Β-glucosidase; Nitrogen nutrition.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200037
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Enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid BJM
Alrumman,Sulaiman A..
Abstract The bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. In this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from Geobacillus stearothermophilus was optimized. The alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. The maximum rate of saccharification was found at a substrate concentration of 4% and enzyme concentration of 30 FPU/g of substrate. The optimum pH and temperature for the bioconversions were 5.0 and 50 °C, respectively, after 24 h of incubation, with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Date palm wastes; Cellulases; Enzymes saccharification; Lactic acid.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100110
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Extracellular enzymes of Fusarium graminearum isolates BABT
Kikot,Gisele Eleonora; Hours,Roque Alberto; Alconada,Teresa María.
Fusarium graminearum isolates from three different agroecological regions in Argentina were examined according to the production of different extracellular enzyme activities of potential biotechnological interest: pectinases (PGase: polygalacturonase and PMGase: polymethylgalacturonase), cellulase (CMCase: carboxymethylcellulase) and hemicellulase (xylanase). The isolates were grown in minimum salt medium supplemented with 0.25% glucose, 0.125% citric pectin and 0.125% oat bran as carbon sources and/or enzyme inducers. PGase activity was detected early (after two days of incubation) in all the cultures; it was found to be the highest for all the isolates. PMGase was high only for those isolates of the II region. CMCase and endoxylanase activities were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular enzymes; Fusarium graminearum; Pectinases; Cellulases; Hemicellulases.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400005
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High cellulolytic activities in filamentous fungi isolated from an extreme oligotrophic subterranean environment (Catão cave) in Brazil Anais da ABC (AABC)
PAULA,CAIO C.P. DE; MONTOYA,QUIMI V.; MEIRELLES,LUCAS A.; FARINAS,CRISTIANE S.; RODRIGUES,ANDRÉ; SELEGHIM,MIRNA H.R..
Abstract Abstract: Isolation and screening of new fungal strains from extreme and understudied environments, such as caves, is a promising approach to find higher yields enzyme producers. Cellulolytic fungal strains isolated from a Brazilian cave were evaluated for their enzymatic production after submerged (SmF) and solid-state fermentation (SSF). After SmF, three strains were selected for their high enzymatic activities: Aspergillus ustus for endoglucanase (4.76 U/mg), Talaromyces bruneus for β-glucosidase (11.71 U/mg) and Aspergillus sp. (CBMAI 1926) for total cellulase (1.70 U/mg). After SSF, these strains, showed better yields compared to the reference strain Aspergillus niger 3T5B8. Aspergillus sp. (CBMAI 1926) stood out as a new species that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulases; Fungu’s cave; Solid state fermentation; Submerged fermentation; Subterranean environment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500601
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Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus BJM
Thomas,Lebin; Ram,Hari; Singh,Ved Pal.
Abstract Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times β-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Cellulases; Conserved signature indels.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200429
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Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material BJM
Cardoso,Wilton Soares; Queiroz,Paula Viana; Tavares,Gabriella Peterlini; Santos,Fernando Almeida; Soares,Filippe Elias de Freitas; Kasuya,Maria Catarina Megumi; Queiroz,José Humberto de.
ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Enzymes; Cellulases; Fungus; Hydrolysis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400879
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Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring BABT
Acharya,Somen; Chaudhary,Anita.
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Thermophiles; Submerged fermentation; Parameter optimization; Cellulases; Hot spring.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400003
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Selection of cellulolytic fungi isolated from diverse substrates BABT
Damaso,Mônica Caramez Triches; Terzi,Selma da Costa; Farias,Antonio Xavier; Oliveira,Ana Carolina Pereira de; Fraga,Marcelo Elias; Couri,Sonia.
The aim of the present work was to select filamentous fungi isolated from diverse substrates to obtain the strains with potential to produce the hydrolytic enzymes. From a total of 215 strains, seven strains from the soils, six from the plants and one from sugarcane bagasse were selected and identified as belonging to the Trichoderma, Penicillium and Aspergillus genera. The best hydrolytic activities obtained by semi-solid fermentation using these strains were approximately: 35; 1; 160; 170 and 120 U/gdm (CMCase, FPase, β-glucosidase, xylanase and polygalacturonase, respectively), demonstrating their potential to synthesize the enzymes compared with the results reported in the literature.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400005
Registros recuperados: 11
Primeira ... 1 ... Última
 

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