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Biopigments from Monascus: strains selection, citrinin production and color stability BABT
Carvalho,Júlio Cesar de; Oishi,Bruno Oliva; Pandey,Ashok; Soccol,Carlos Ricardo.
Fungi form the genus Monascus are a promising source for natural color additives. However, before effectively applying Monascus to foods, it is important to select strains which produce large amounts of biopigments but little or no citrinin, a mycotoxin usually also produced by these fungi. Also, color stability of these pigments should be properly investigated. In order to compare Monascus strains for biopigment production in solid substrate fermentation (SSF), 4 strains (NRRL 1991, NRRL 2897, CCT 3802 and LPB 31) were cultivated over PDA in Petri dishes, and compared for radial growth velocity. Also, these strains were cultivated over cooked rice, and compared in relation to their capacity to produce biopigments and citrinin. The results showed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monascus; Biopigment; Citrinin; Solid-substrate fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800004
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Citrininotoxinogenicity of Penicillium spp. isolated from decaying apples BJM
Pepeljnjak,Stjepan; Šegvic,Maja; Ozegovic,Ladislav.
A study on the occurrence of citrinin and citrinin production ability of Penicillium spp. isolated from decaying apples collected from households in Croatia was carried out. Among 100 samples of apples, 37 strains of Penicillium spp. were found, including P. expansum, P. roqueforti, P. implicatum and P. purpurogenum. Citrinin production in liquid yeast medium by 11 strains of P. expansum varied in a range of 0.07 to 9.00 mg.kg-1. Citrinin was isolated from 19% of apple samples in range of 0.05 to 0.24 mg.kg-1. Antimicrobial activity of isolated citrinin, evaluated through tests on Bacillus subtilis, presented inhibitory zones varying from 5 mm to 1 cm. Minimal inhibitory concentrations (MIC) were 0.0072 µg.mL-1 for bacteriostatic effect, and 0.0144 µg.mL-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrinin; Penicillium expansum; Decaying apples; Antimicrobial activity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200007
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Effect of citrinin and in association with aflatoxin B1 on the infectivity and proliferation of Toxoplasma gondii in vitro BJID
Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos.
Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007
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Quantification of mycophenolic acid and citrinin produced by Penicillium sp. using 1H NMR. Repositório Alice
VALENTE, A. M. M. P.; BOFFO, E. F.; MELO, I. S. de; FERREIRA, A. G..
Abstract: This study proposes a simple methodology to construct the production curves of mycophenolic acid by Penicillium sp. (CASP5) and citrinin by Penicillium sp. (CATL1.1) in the crude extract, without any purification. The quantification of the compounds was done by 1H nuclear magnetic resonance (NMR) using the signal integration and an internal standard, N,N-dimethylformamide. Fungi were cultivated for a period of 20 days and quantification of the metabolites in the extracts was done starting from time zero, 2 days and after this period in an interval of 4 days. The maximum production of mycophenolic acid and citrinin was obtained at 12 and 8 days of fermentation, respectively. These results show that the 1H NMR technique was efficient to define the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycophenolic acid; Penicillium sp.; NMR; Fungo; Análise de laboratório; Citrinin; Nuclear magnetic resonance spectroscopy.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1000338
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Toxigenic fungi in beans (Phaseolus vulgaris L.) classes black and color cultivated in the State of Santa Catarina, Brazil BJM
Costa,Léa Luzia Freitas; Scussel,Vildes Maria.
Toxigenic fungi were studied in beans (Phaseolus vulgaris L.) of Classes black and color, cultivated in different regions of the State of Santa Catarina, south region of Brazil. The mean counts of filamentous fungi were 2.8 x 103 and 6.7 x 103 CFU/g for beans Classes black and color, respectively. Penicillium spp., Aspergillus spp. and Phoma spp. were the most frequent genera isolated, followed by Ryzopus spp., Alternaria spp., Helminthosporium spp., Cladosporium spp., Botrytis spp., Fusarium spp., Trichoderma spp., Curvularia spp. and Dreschelera spp. Among beans Class black, 24.6% of the Aspergillus strains produced mycotoxins: 13.1% produced aflatoxins (AFs); 11.5% produced ochratoxin A (OTA) and 28.9% of Penicillium produced citrinin (CTR). On the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beans; Phaseolus; Toxigenic fungi; Aflatoxin; Ochratoxin A; Citrinin.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200008
Registros recuperados: 5
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