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Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France ArchiMer
Azandegbe, Afi; Gamier, Matthieu; Andrieux, Francoise; Kerouel, Roger; Philippon, Xavier; Nicolas, Jean-louis.
Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V....
Tipo: Text Palavras-chave: Vibrio aestuarianus; Crassostrea gigas Sediment; Seasonal abundance; Colony-blot.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12813/9756.pdf
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