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Registros recuperados: 10
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Actividad de polvo, extractos y aceite esencial de Peumus boldus Molina solos y en mezcla con Bacillus thuringiensis Berliner contra Spodoptera frugiperda (J.E. Smith) y Helicoverpa zea (Boddie). Colegio de Postgraduados
Silva Aguayo, Gonzalo Iván.
Se evaluaron en condiciones de laboratorio e invernadero las propiedades insecticidas del polvo, aceite esencial y extracto en cloruro de metileno de Boldo (Peumus boldus Molina), además del alcaloide boldina y el terpeno 1-8-cineol (eucaliptol), solos y en combinación con Bacillus thuringiensis Berliner contra larvas de Spodoptera frugiperda (J.E. Smith) y Helicoverpa zea Boddie. Se realizaron bioensayos para evaluar mortalidad, efecto en el ciclo de vida y las preferencias alimenticias de larvas neonatas y de tercer instar e inhibición de la alimentación y oviposición. El diseño experimental fue de bloques completos al azar con 7 tratamientos y 20 repeticiones. La mayor toxicidad se manifestó con el extracto que a una concentración de 8% tuvo una...
Palavras-chave: Gusano cogollero; Gusano elotero; Monoterpenos; Insecticidas vegetales; Cry1Aa; Cry1Ac; Fall armyworm; Corn armyworm; Monoterpenes; Botanical insecticides; Doctorado; Entomología y Acarología.
Ano: 2010 URL: http://hdl.handle.net/10521/201
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Actividad de polvo, extractos y aceite esencial de Peumus boldus Molina solos y en mezcla con Bacillus thuringiensis Berliner contra Spodoptera frugiperda (J.E. Smith) y Helicoverpa zea (Boddie). Colegio de Postgraduados
Silva Aguayo, Gonzalo Iván.
Se evaluaron en condiciones de laboratorio e invernadero las propiedades insecticidas del polvo, aceite esencial y extracto en cloruro de metileno de Boldo (Peumus boldus Molina), además del alcaloide boldina y el terpeno 1-8-cineol (eucaliptol), solos y en combinación con Bacillus thuringiensis Berliner contra larvas de Spodoptera frugiperda (J.E. Smith) y Helicoverpa zea Boddie. Se realizaron bioensayos para evaluar mortalidad, efecto en el ciclo de vida y las preferencias alimenticias de larvas neonatas y de tercer instar e inhibición de la alimentación y oviposición. El diseño experimental fue de bloques completos al azar con 7 tratamientos y 20 repeticiones. La mayor toxicidad se manifestó con el extracto que a una concentración de 8% tuvo una...
Palavras-chave: Gusano cogollero; Gusano elotero; Monoterpenos; Insecticidas vegetales; Cry1Aa; Cry1Ac; Fall armyworm; Corn armyworm; Monoterpenes; Botanical insecticides; Doctorado; Entomología y Acarología.
Ano: 2010 URL: http://hdl.handle.net/10521/201
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Biologia comparada e comportamento de Tetranychus urticae Koch (Acari: Tetranychidae) e Phytoseiulus macropilis (Banks) (Acari: Phytoseiidae) em algodoeiro bollgardTM e Isolinha não-Transgênica Neotropical Entomology
Esteves Filho,Alberto B; Oliveira,José V de; Torres,Jorge B; Gondim Jr,Manoel G C.
The two spotted spider mite, Tetranychus urticae Koch, is a nontarget herbivore of Bt-cotton, but acquires and accumulates higher levels of Cry toxin than that expressed by transgenic plants. This work investigated the development and reproduction of T. urticae and of the predator Phytoseiulus macropilis Banks, during three successive generations looking for potential nontarget effect. In addition, behavioral studies on feeding preference, oviposition, and predation were carried out on Bt and non-Bt cottons. The development and reproduction of T. urticae and P. macropilis was conducted using leaf discs of Bt and non-Bt cottons. Arena containing leaf discs from both cotton types connected by a slide coverslip were also used in the behavioral studies....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic cotton; Cry1Ac; Tritrophic interaction; Nontarget arthropod.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000300005
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Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain Neotropical Entomology
Sauka,Diego H; Basurto-Ríos,Regina E; Ibarra,Jorge E; Benintende,Graciela B.
We report the characterization of an Argentine isolate of Bacillus thuringiensis (INTA TA24-6) similar to the HD-1 strain, which harbors a cryptic cry2Ab gene that apparently is transcribed but not translated into a protein. INTA TA24-6 showed a Rep-PCR pattern identical to the HD-1 strain, a plasmid pattern that resembled that of this strain and cry1 and cry2 genes as HD-1. Screening of cry1 and cry2 genes showed that INTA TA24-6 harbors only cry1Ac and cry2Ab genes. Furthermore, crystalline inclusions of INTA TA24-6 exhibit a bipyramidal shape, typical of Lepidoptera-active B. thuringiensis strains, containing a major protein of ca. 130 kDa toxic to Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae. Neither the flat-square to cuboidal crystal nor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptic gene; Cry1Ac; Cry2Ab; Epinotia aporema.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000500016
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Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton Biol. Res.
Muzaffar,Adnan; Kiani,Sarfraz; Khan,Muhammad Azmat Ullah; Rao,Abdul Qayyum; Ali,Arfan; Awan,Mudassar Fareed; Iqbal,Adnan; Nasir,Idrees Ahmad; Shahid,Ahmad Ali; Husnain,Tayyab.
BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed...
Tipo: Journal article Palavras-chave: Chloroplast transient peptide; Cry1Ac; Cry2A; Bt; CTP; Cry genes; Endotoxins; GUS; Transgenic plants.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100014
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EXPRESIÓN DE LA PROTEÍNA Cry1Ac EN TEJIDOS DE LÍNEASTRANSGÉNICAS DE PAPA (Solanum tuberosum spp. ANDÍGENA)VAR. DIACOL CAPIRO. Acta biol.Colomb.
VANEGAS ARAUJO,PABLO ANDRÉS; BLANCO MARTÍNEZ,JENNIFER TERESA; CHAPARRO-GIRALDO,ALEJANDRO.
La papa (Solanum sp.) es el cuarto producto alimenticio más importante en el mundo. En Colombia anualmente se producen alrededor de 2,8 millones de toneladas, sirviendo como sustento económico a 90.000 familias. En el país, Tecia solanivora genera el mayor impacto económico en el cultivo con pérdidas de hasta el 100% en la producción de tubérculos. El fitomejoramiento vía introducción de genes Cry, que codifican para cristales proteicos insecticidas, constituye una alternativa para reducir el ataque de insectos en cultivos de interés comercial. En este trabajo se caracterizó la inserción, transcripción y expresión del gen Cry1Ac en diferentes tejidos y en tres etapas del desarrollo para dos líneas transgénicas de Solanumtuberosum spp. andígena variedad...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. tuberosum; Diacol Capiro; Cry1Ac; Caracterización molecular; Cultivos transgénicos.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000200008
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Fitness cost of resistance to Bacillus thuringiensis in velvetbean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) Rev. Bras. entomol.
Sosa-Gómez,Daniel Ricardo; Miranda,José Ednilson.
Selection pressure to obtain resistant genotypes can result in fitness cost. In this study, we report the effects of the selection pressure of a commercial formulation of Bacillus thuringiensis on biological aspects of a Dipel-resistant strain of velvetbean caterpillar, Anticarsia gemmatalis Hübner. Comparisons of Dipel-resistant and susceptible individuals revealed significant differences in pupal weight and larval development time. Both strains (Dipel-resistant and susceptible) were susceptible to Cry1Ac toxin expressed in foliar cotton tissues. Resistant and susceptible strains showed low survival rates of 22.5% and 51.2%, respectively, when fed with Greene diet containing Bt-cotton. Larvae bioassayed after three laboratory generations presented lower...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Toxin; Cry1Ac; Dipel-resistant; Strain.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000300014
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Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis Electron. J. Biotechnol.
Rahman,Mahmood-ur; Rashid,Hamza; Shahid,Ahmad Ali; Bashir,Khurram; Husnain,Tayyab; Riazuddin,Sheikh.
Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005). Homozygous lines were selected and analyzed for insect resistance, morphological, physiochemical properties and risk assessment studies. After artificial infestation of target insects, the transgenic plants showed significant resistance. Data were recorded in terms of dead hearts and white heads at vegetative and flowering stage respectively. Transgenic lines showed up to 100 and 96% resistance against yellow stem borer at vegetative and flowering stages,...
Tipo: Journal article Palavras-chave: Bio-safety; Cry1Ac; Cry2A; Gene flow; Field trials; Gene pyramiding; Insect resistance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008
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Protocolo para avaliação dos efeitos de proteínas tóxicas a insetos-praga sobre a abelha sem ferrão Trigona spinipes (Hymenoptera, Apidae, Meliponina). Infoteca-e
DIAS, S. C.; BROD, C.; SUJII, E. R.; FONTES, E. M. G.; PIRES, C. S. S..
bitstream/CENARGEN/29521/1/bp197.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Plantas transgênicas; Cry1Ac; Abelhas sem ferrão; Bioensaios.; Controle Biológico..
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/189392
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STUDY OF GENE FLOW FROM GM COTTON (Gossypium hirsutum) VARIETIES IN "EL ESPINAL" (TOLIMA, COLOMBIA) Acta biol.Colomb.
RACHE CARDENAL,LEIDY YANIRA; MORA-OBERLAENDER,JULIAN; CHAPARRO-GIRALDO,ALEJANDRO.
In 2009, 4088 hectares of genetically modified (GM) cotton were planted in Tolima (Colombia), however there is some uncertainty about containment measures needed to prevent the flow of pollen and seed from regulated GM fields into adjacent fields. In this study, the gene flow from GM cotton varieties to conventional or feral cotton plants via seed and pollen was evaluated. ImmunostripTM, PCR and ELISA assays were used to detect gene flow. Fifty six refuges, 27 fields with conventional cotton and four feral individuals of the enterprise "Remolinos Inc." located in El Espinal (Tolima) were analyzed in the first half of 2010. The results indicated seed mediated gene flow in 45 refuges (80.4 %) and 26 fields with conventional cotton (96 %), besides pollen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosafety; Cry1Ac; GM crops; Transgenic cotton.
Ano: 2013 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000300008
Registros recuperados: 10
Primeira ... 1 ... Última
 

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