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Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum Electron. J. Biotechnol.
Ding,Yu Fei; Wang,Chuan Tang; Tang,Yue Yi; Wang,Xiu Zhen; Wu,Qi; Hu,Dong Qing; Yu,Hong Tao; Zhang,Jian Cheng; Cui,Feng Gao; Song,Guo Sheng; Gao,Hua Yuan; Yu,Shan Lin.
Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively,...
Tipo: Journal article Palavras-chave: Arachis hypogaea; ARF; Cyclophilin; Differentially expressed genes; Genefishing; Ralstonia solanacearum.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500001
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Relative expression of CYP and Arabinogalactan genes in soybean seed coats Rev. Ciênc. Agron.
Bahry,Carlos André; Venske,Eduardo; Perboni,Anelise Tessari; Zimmer,Paulo Dejalma.
ABSTRACT The aim of this study was to evaluate the relative expression of two candidate genes, CYP (cyclophilin) and Arabinogalactan, possibly involved with seed quality, in contrasting soybean seed coats. The following genotypes were used: BMX Potência RR and CD 202, both with yellow coats, and TP and IAC, with black coats. The relative expression of the genes was evaluated by qPCR in seven development phases of the coats of the four genotypes at 25; 30; 35; 40; 45; 50 and 55 days after anthesis. The design was completely randomized, with three replicates. The data were submitted to analysis of variance and the means were compared via the Scott Knott test, at 5% probability. The CYP gene showed greater expression in the intermediate stages of development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contrasting genotypes; Candidate genes; Cyclophilin; QPCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100078
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