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Registros recuperados: 7
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An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses. Repositório Alice
SANTOS, T. B. dos; SOARES, J. D. M.; LIMA, J. E.; SILVA, J. C.; IVAMOTO, S. T.; BABA, V. Y.; SOUZA, S. G. H.; LORENZETTI, A. P. R.; PASCHOAL, A. R.; MEDA, A. R.; NISHIYAMA JÚNIOR, M. Y.; OLIVEIRA, U. C. de; MOKOCHINSKI, J. B.; GUYOT, R.; JUNQUEIRA-DE-AZEVEDO, I. L. M.; FIGUEIR, A. V. O.; MAZZAFERA, P.; R. JÚNIO, O.; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S..
Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nitrogen transport; Differential gene expression; RNA-seq; MicroRNA.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108713
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Differential morphological, physiological, and molecular responses to water deficit stress in sugarcane. Repositório Alice
PEDROZO, C. A.; JIFON, J.; BARBOSA, M. H. P.; SILVA, J. A. da; PARK, J.; GRACIA, N. S..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Differential gene expression; Water deficit stress; Saccharum spp.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1025986
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Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum. Repositório Alice
CORDEIRO, I. B.; CASTRO, D. P.; NOGUEIRA, P. P. O.; ANGELO, P. C. S.; NOGUEIRA, P. A.; GONÇALVES, J. F. C.; PEREIRA, A. M. R. F.; GARCIA, J. S.; SOUZA, G. H. M. F.; ARRUDA, M. A. Z.; EBERLIN, M. N.; ASTOLFI-FILHO, S.; ANDRADE, E. V.; LÓPEZ-LOZANO, J. L..
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: CIRCE; Chaperones; Heat shock response; Differential gene expression.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974472
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Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. Repositório Alice
MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; ALSARELLA CARAZZOLLE, M.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P..
BACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Candidate gene; Coffee; Differential gene expression; RNA-Seq; Real-time PCR (RT-qPCR); Drought; Coffea arabica.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1043710
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Identification of General Patterns of Sex-Biased Expression in Daphnia, a Genus with Environmental Sex Determination ArchiMer
Molinier, Cecile; Reisser, Celine; Fields, Peter; Segard, Adeline; Galimov, Yan; Haag, Christoph R..
Daphnia reproduce by cyclic-parthenogenesis, where phases of asexual reproduction are intermitted by sexual production of diapause stages. This life cycle, together with environmental sex determination, allow the comparison of gene expression between genetically identical males and females. We investigated gene expression differences between males and females in four genotypes of Daphnia magna and compared the results with published data on sex-biased gene expression in two other Daphnia species, each representing one of the major phylogenetic clades within the genus. We found that 42% of all annotated genes showed sex-biased expression in D. magna. This proportion is similar both to estimates from other Daphnia species as well as from species with genetic...
Tipo: Text Palavras-chave: Differential gene expression; Males; Females; Daphnia; ESD; Genetics of Sex.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00442/55322/56832.pdf
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RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct Biocell
Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C..
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005
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Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Repositório Alice
BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M..
Peanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Amendoim; Resistência a seca; Gene tolerante; Cultura resistente a seca; Biologia molecular; SSHlibraries; 454 Sequencing; Differential gene expression; Dry-down; Peanut wild relatives; QRT-PCR.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035448
Registros recuperados: 7
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