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Registros recuperados: 19
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Adaptative responses of E-coli to marine environmental stresses. a modelling approach based on viability and dormancy concepts ArchiMer
Martin, Y; Troussellier, Marc; Bonnefont, Jl.
In an attempt to synthesize conceptual and experimental information on the behaviour of enteric bacteria in seawater, a mathematical model has been developed. This model is based on changes in intracellular metabolisable components depending an physiological responses of the cell when subjected to nutrient starvation, salinity stress and solar radiation. Following a strategy which takes into account short- and mid-term physiological adaptation and reversal processes, the cells can develop in different states: culturable (B-1), viable and definitively nonculturable (B-2), and reversibly dormant (B-3) cells. Model parameters were deduced either from original microcosm experiments, from literature data or calibration procedures. Validation of the model was...
Tipo: Text Palavras-chave: Escherichia coli; Eau de mer; Stress; États cellulaires; Modélisation; Escherichia coli; Seawater; Stress; Cellular states; Modelling.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00325/43646/43224.pdf
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Analyse, distribution, production et dégradation d’esters de phtalates (PAEs) en zone côtière méditerranéenne ArchiMer
Paluselli, Andrea.
Phthalic Acid Esters (PAEs) are a group of emerging organic contaminants that have become a serious issue because of their ubiquitous presence on the marine environment worldwide and for the endocrine disrupting effects in animals and humans. However, little is known about their distribution in the Ocean, mainly because of analytical difficulties and the high possibility of ambient sample contamination. Plastic debris in marine environment includes resin pellets, macro- and microplastic fragments, and contains additives such as PAEs that might be released in the aquatic environment in unknown proportion. In our work, we improved an analytical method for the determination of 8 PAEs, at trace levels in marine and fresh waters. In the first part of the...
Tipo: Text Palavras-chave: Phtalates; DEHP; Eau de mer; Perturbateurs endocriniens; Microplastiques; Microplastics; Endocrine disruptors; Seawater; Phthalates; Dehp.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00497/60904/64252.pdf
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Bilan 1992 de la contamination des côtes françaises par les composés organostanniques ArchiMer
Michel, Pierre; Averty, Bernard.
Au cours du printemps 1992, un échantillonnage systématique a permis d'évaluer le niveau de la contamination des côtes françaises par les composés organostanniques. Le tributyl étain (TBT) a été mesuré dans la phase dissoute avec ses produits de dégradation dibutyl étain (DBT) et monobutyl étain (MBT). Les concentrations mesurées restent importantes dans les zones portuaires et les zones de mélanges. Ces concentrations sont le plus souvent excessives lorsqu'on se réfère au seuil toxique de 1ng.1-1 pour lequel des effets sont connus sur l'huître et certains autres mollusques. Les trois façades maritimes françaises ont été contôlées ; c'est en Méditerranée que l'on peut observer les plus fortes concentrations. En plus du TBT et de ses métabolites, on a aussi...
Tipo: Text Palavras-chave: Tributyl étain; Dibutyl étain; Monobutyl étain; Triphényl étain; Composés organostanniques; Eau de mer; France.; Tributyltin; Dibutyltin; Monobutyltin; Triphenyltin; Organotin compounds; Seawater; French coasts.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00104/21530/19111.pdf
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Compréhension des mécanismes d'adhésion des biofilms en milieu marin en vue de la conception de nouveaux moyens de prévention ArchiMer
Rubio, Celine.
Biofilm formation on surfaces immersed in sea water induces equipment damages. Recently, studies focused on the environmental impact of antifouling paints have shown that some active chemical compounds are harmful to the aquatic environment. Hence, it appears fundamental to study physico-chemical interactions existing between marine bacteria and various substrata to conceive new ecological products or antifouling processes. First, we model an artificial primary organic film, formed by the adsorption of BSA and polysaccharide, as close as the one formed in natural seawater. This organic film is heterogeneous (only 42% of the surface is covered) and organic adsorption occurs in a multi-layer scheme, molecules bounded each others by calcium and magnesium...
Tipo: Text Palavras-chave: Sea water; Marine bacteria; Physico chemical characterisation; MATS; XPS; IRAS; Adsorption; Bacterial adhesion; Biofim; Eau de mer; Bactéries marines; Caractérisation physicochimique; MATS; XPS; IRRAS; Adsorption; Adhésion bactérienne; Biofilm.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/these-222.pdf
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Détection et étude de la stabilité de l'ADN de virus de type herpès infectant les huîtres dans des échantillons d'eau ArchiMer
Vigneron, Vassilia.
Depuis 1991, des mortalités massives associées à des infections à herpès virus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Les études ont porté essentiellement sur la mise en évidence de la présence de ce type de virus chez les coquillages d'intérêt économique. Actuellement, l'intérêt se porte sur la persistance du virus dans le milieu marin, et cela dans le but de déterminer si le milieu constitue ou non un moyen de contamination des animaux. Le travail a constitué en une étude sur la persistance de la détection de l'ADN d'herpès virus (OsHV-1) afin de mieux appréhender les modes de dissémination et de transmission du virus. Dans un premier temps, la stabilité de l'ADN purifié et...
Tipo: Text Palavras-chave: Herpèsvirus; Eau de mer; Détection; PCR; Persistance; Stabilité.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00032/14350/11634.pdf
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Devenir des virus entériques en mer et influence des facteurs environnementaux ArchiMer
Gantzer, Christophe; Dubois, Éric; Crance, Jean-marc; Billaudel, Sylviane; Kopecka, Helena; Schwartzbrod, Louis; Pommepuy, Monique; Le Guyader, Françoise.
To improve the knowledge of the survival of enteric viruses in a marine environment, the influence of physico-chemical parameters (temperature, UV, salinity) on the survival of infectious poliovirus 1 and hepatitis A Virus (HAV) in seawater was first studied, the influence of suspended solids (SS) on poliovirus adsorption and survival in seawater was then evaluated and the detection of rotavirus genome in environmental samples (shellfish, river water, treated wastewater) was finally investigated. The results show that temperature has a major impact on virus survival in seawater as the time necessary to inactivate 90 % of the virus (T-90) is 671 days at 4 degrees C and only 25 days at 25 degrees C. Ultraviolet light (42 mW s cm(-2)) rapidly inactivates...
Tipo: Text Palavras-chave: Environmental factors; Survival; Seawater; Enteric viruses; Facteurs environnementaux; Survie; Eau de mer; Virus entériques.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-846.pdf
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Durabilité des composites pour énergie marine renouvelable ArchiMer
Davies, Peter; Boisseau, A.; Choqueuse, Dominique; Thiebaud, F.; Perreux, D..
Composites will play an essential role in the development of ocean energy conversion systems, and their long term durability is critical to the success of these structures. Composites have been widely used in marine applications and in structures highly loaded in fatigue such as wind turbine blades. However, very few data are available to evaluate their performance under conditions which combine long term immersion in sea water and sustained cyclic loading. In this study composites reinforced with three different types of glass fibre and the same epoxy resin have been tested under these conditions, and the influence of aging on both static and fatigue behaviour has been quantified. Fibre type, resin and fibre/resin interface all influence the fatigue...
Tipo: Text Palavras-chave: Fatigue; Eau de mer; Énergie marine; Hydrolienne; Pale; Fatigue; Sea water; Ocean energy; Tidal turbine; Blade.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00480/59161/61790.pdf
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Estimation par spectrofluométrie des concentrations d'hydrocarbures dans l'eau de mer en Manche Occidentale à la suite du naufrage de l"AMOCO CADIZ" du 30 mars au 18 avril 1978 ArchiMer
Marchand, Michel.
The oil .pollution,resulting from the "AMOCO CADIZ" tanker spill on the Brittany coast of North Finistere-has affected a very large section of the western Channel, limited for the period covering March 30 to April 18 to the west by the 5°W meridian, to the east by the St-Brieuc Bay, to the north by the 49°20' parallel. The contents of hydrocarbons observed in sea water at different levels show that the whole of water column is contaminated by hy­drocarbons and indicate very probably the pollution of the sedimentary phase in the areas, hited by the drifting sheets.
Tipo: Text Palavras-chave: Amoco Cadiz; Hydrocarbures; Eau de mer; Spectrofluorométrie; Manche; Amoco Cadiz; Sea-water; Spectrofluorometry; English Channel.
Ano: 1978 URL: http://archimer.ifremer.fr/doc/00306/41717/40928.pdf
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Etat chimique des sédiments et influence d'une extraction de granulats sur l'état chimique de l'eau de mer dans le cadre du PER GMH - Etude SCOOTER. Etude sur la remobilisation de Sédiments potentiellement COntaminés induite par une OpéraTion d’ExtRaction de granulats en baie de Seine ArchiMer
Menet-nedelec, Florence; Chiffoleau, Jean-francois; Riou, Philippe; Maheux, Frank; Pierre-duplessix, Olivier; Rabiller, Emilie; Simon, Benjamin.
Dans les mers côtières où les données des réseaux de surveillance montrent que les sédiments fins en surface contiennent des contaminants, la remobilisation de ces matériaux à l’occasion d’extractions de granulats, qui génère un panache turbide, pourrait influencer la qualité de l’eau. La baie de Seine orientale est sous l’influence du panache de la Seine, l’un des fleuves les plus contaminés d’Europe. Cette étude avait pour objectif d’examiner la remobilisation des métaux traces et de contaminants organiques des sédiments vers la surface pendant et après une extraction de granulats à travers leur comportement entre la fraction totale et dissoute. Pour cela, l’expérimentation a été réalisée lors d’un essai d’extraction dans le cadre du Permis Exclusif de...
Tipo: Text Palavras-chave: Baie de Seine; Métaux traces; Contaminants organiques; Sédiment; Eau de mer; Panache turbide; Extraction de granulats..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00310/42078/41381.pdf
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Etude de la dynamique des populations zooplanctoniques au sein d'une claire ostréicole ArchiMer
Sajus, Marie-celine.
Marteilia refringens est un protozoaire parasite de l'huître plate Ostrea edulis, à l'origine de mortalités dramatiques en élevage depuis les années 1970. En zone endémique, le seul moyen de contrôle de la marteiliose, est une gestion du risque de contamination. Dans ce cadre, la connaissance du cycle parasitaire de Marteilia refringens est primordiale. Les travaux réalisés à ce sujet ont permis de poser l'hypothèse d'un cycle hétéroxène. Le parasite a été détecté par PCR puis localisé au sein des tissus par hybridation in situ au sein d'une espèce de copépode Calanoide prélevée dans une claire ostréicole. A ce stade, cette espèces peut être considéré comme un hôte de Marteilia refringens. Notre étude consistait à mettre en évidence la dynamique des...
Tipo: Text Palavras-chave: Marteilia refringens; Parasitologie; Huître plate; Ostrea edulis; Eau de mer; Zooplancton; Claires ostréicoles.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00032/14351/11635.pdf
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Flux et usages de l'eau de mer dans les marais salés endigués Charentais : Cas du marais salé endigué de l'île de Ré ArchiMer
Paticat, Francois.
The aim of the study is to determine how seawater is used in Ré island marine ponds (Charente-Maritime, France). Thus, we assessed the ecosystem functioning, estimated its historical evolution and determined the most suitable ways to improve current management processes. The knowledge of seawater management is essential to ensure wetland future and meet the numerous interests of wetland stakeholders, especially in term of seawater sharing. Consequently, it is necessary to consider the Ré island marine embanked wetland through its natural as well as human characteristics. Our work followed two research axes. First, a survey and a description of wetland activities were realized through their management types and their water use features, knowing that human...
Tipo: Text Palavras-chave: Ré Island; Activities; Management; Saltwater; Flows; Coastline; Île de Ré; Activités; Gestion; Eau de mer; Flux; Littoral.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-4518.pdf
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Les métaux (Cd, Cu, Pb et Zn) dans la production des microalgues sur différents milieux de culture : biodisponibilité, bioaccumulation et impact physiologique ArchiMer
Gagneux-moreaux, Sindy.
The quality and the productivity of microalgal cultures depend on different physicochemical factors like salinity, pH, nutrient and trace element (including trace metals) concentration in the culture medium. During this doctoral study, the bioavailability of four metals for microalgae was tested in coastal seawater and several salt groundwaters. The bioavailability of a metal depends on its own total concentration as well as that of ligands in the medium, and on the relative affinity of each ligand towards the given metal. After a comparative study of the quality of algae grown in coastal seawater and salt groundwaters, the complexation capacities of these media were studied to allow a better understanding of the observed variations of metal...
Tipo: Text Palavras-chave: Metal speciation; Artifical medium DAM; Salt groundwater; Coastal seawater; Tetraselmis suecica; Skeletonema costatum; Phaeodactylum tricornutum; Haslea ostrearia; Spéciation métallique; Milieu artificiel DAM; Eau souterraine salée; Eau de mer; Tetraselmis suecica; Skeletonema costatum; Phaeodactylum tricornutum; Haslea ostrearia.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-3128.pdf
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Multi-Element Determination of Trace Elements in Natural Water Reference Materials by ICP-SFMS after Tm Addition and Iron Co-precipitation ArchiMer
Bayon, Germain; Birot, Dominique; Bollinger, Claire; Barrat, Jean Alix.
We report on an improved method for determining trace element abundances in seawater and other natural waters. The analytical procedure involves co-precipitation on iron hydroxides after addition of a Tm spike, and measurement by inductively coupled plasma-sector field mass spectrometry (ICP-SFMS). The validity of the method was assessed through a series of co-precipitation experiments, using ultra-diluted solutions of a certified rock reference material (BIR-1). Results obtained for four natural water reference materials (NASS-5, CASS-4, SLEW-3, SLRS-4) are in agreement with published working values for rare earth elements, yttrium, vanadium and, when available, for hafnium, zirconium, thorium and scandium. A set of proposed values with uncertainties...
Tipo: Text Palavras-chave: Seawater; Reference material; ICP-MS; Trace elements; Rare earth elements; Eau de mer; Materiau de reference; ICP-MS; Elements traces; Terres Rares.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14189/18000.pdf
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Réaction de réduction de l'oxygène sur les aciers inoxydables en eau de mer naturelle. Influence du biofilm sur les processus de corrosion ArchiMer
Le Bozec, Nathalie.
iofilm development on stainless steels immersed in natural seawater can have prejudicial consequences on the resistance of these materials to corrosion . The goal of the present study was to get more precise information on the corrosion processes, and especially on the oxygen reduction reaction. As the reaction is linked to the stainless steel surface state, the characterisation of the oxides films (composition, structure, thickness...) is essential to understand the mechanisms and the oxygen reduction kinetic. The first aim of the study has been to correlate the oxygen reduction processes with the characteristics of the oxides layer as a function of the alloy surface treatment (mechanical polishing, electrochemical passivation and prereduction, chemical...
Tipo: Text Palavras-chave: Hydrogen peroxide; Oxides layer; Biofilm; Oxygen reduction; Corrosion; Seawater; Stainless steels; Peroxyde d'hydrogène; Films d'oxydes; Biofilm; Réduction de l'oxygène; Corrosion; Eau de mer; Aciers inoxydables.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2004/these-223.pdf
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Recherche par PCR d'OsHV-1 (Ostreid Herpesvirus type 1), dans des échantillons d'eau de claires ostréicoles ArchiMer
Solliec, Gaelle.
Depuis 1991, des mortalités massives associées à des infections à herpèsvirus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Un nouveau groupe au sein de la famille des Herpesviridae a été créé afin d'y intégrer les herpèsvirus d'invertébrés marins avec actuellement pour unique représentant le virus infectant l'huître creuse, Crassostrea gigas, OsHV-1. Des études ont porté sur la mise en évidence d'OsHV-1 chez les coquillages d'intérêt économique après développement d'outils de diagnostic rapides et fiables. Ainsi, la maladie est aujourd'hui assez bien connue. Toutefois, le devenir d'OsHV-1 dans le milieu extérieur n'a fait l'objet que de peu d'études. Aussi, l'objectif principal de ce...
Tipo: Text Palavras-chave: Herpèsvirus; Bivalves; Huître creuse; OsHV-1; Eau de mer; Claire ostréicole; Persistance; Détection; Spécificité; Champignons marins; Isolement; Virus de type herpès.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00025/13644/10726.pdf
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Recherches d'ADN d'hepès virus infectant les mollusques dans les populations d'huîtres en milieu naturel et dans leur environnement ArchiMer
Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan.
The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural...
Tipo: Text Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf
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Responses of enteric bacteria to environmental stresses in seawater ArchiMer
Troussellier, Marc; Bonnefont, Jean-luc; Courties, Claude; Derrien, Annick; Dupray, Elizabeth; Gauthier, Michel; Gourmelon, Michele; Joux, Fabien; Lebaron, Philippe; Martin, Yvan; Pommepuy, Monique.
The effects of different environmental factors (nutrient deprivation, hyperosmotic shock, exposure to light) on enteric bacteria which have been transferred into the marine environment, have been studied experimentally (microcosms) by considering demographic, physiological and genetic responses in Escherichia coli or Salmonella typhimurium populations. Short-term experiments (less than or equal to 48 h) showed that nutrient deprivation induced limited changes in measured bacteriological variables, but when combined with hyperosmotic shock, it results in an energy charge decrease and inactivation of membrane transport. Light exposure mainly affects the colony-forming capacity of bacterial populations. Combining different stress factors confirmed the rapid...
Tipo: Text Palavras-chave: Genetics; Cellular states; Physiology; Seawater; Enteric bacteria; Génétique; Etats cellulaires; Physiologie; Eau de mer; Entérobactéries.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-845.pdf
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Résultats préliminaires de la pollution prétrolière par l'AMOCO CADIZ. Teneurs en hydrocarbures totaux dans les eaux de mer, de la Rade de Brest à la presqu'île du Cotentin (mars - avril 1978) ArchiMer
M0rel, G.; Courtot, P..
Results of preliminary measurements of sea water total hydrocarbon , content by infra-red show normal values in Bay of Brest but values between 100 and 200 ug/1 in North-Finistere sea waters during April 1978. Significant increases of these values are observed with increasing depths.
Tipo: Text Palavras-chave: Hydrocarbures totaux; Méthode infra-rouge; Eau de mer; Amoco Cadiz; Nord-Finistère; Total hydrocarbons; Infra-red method; Sea water; . Amoco Cadiz; Nord-Finistère.
Ano: 1978 URL: https://archimer.ifremer.fr/doc/00306/41718/40929.pdf
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The determination of polycyclic aromatic hydrocarbons in seawater from the Fluxmanche transect (Dover Strait) ArchiMer
Law, Rj; Whinnett, Ja.
Concentrations of a range of polycyclic aromatic hydrocarbons (PAH) were determined by means of coupled gas chromatography-mass spectrometry (GCMS). Water samples were collected, using a contamination-free sampling device, at two depths (Im subsurface and 3 m above the seabed) at each of the six stations established in the Dover Strait. The stations were occupied during June and October 1990, and July 1991. Concentrations of PAH were often close to or below the limits of detection for samples of 10.8 1 volume, although a number of samples exhibited high concentrations (of naphthalenes in particular) which were apparently derived from shipping inputs in the Dover Strait. Future work will require the development of methods with lower limits of detection, and...
Tipo: Text Palavras-chave: Hydrocarbures polycycliques aromatiques; Eau de mer; Fluxmanche; CG/SM; Pas-de-Calais; POLYCYCLIC AROMATIC HYDROCARBONS; SEAWATER; FLUXMANCHE; GC/MS; DOVER STRAIT.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00323/43391/43045.pdf
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