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Registros recuperados: 11
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Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study International Journal of Morphology
Bohlouli,Somayeh; Khazaei,Mozafar; Rabzia,Arezou; Khazaei,Mohammad Rasool; Sadeghi,Ehsan.
Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman with endometriosis. The aim of this study was to determine adiponectin effect on nitric oxide secretion by cultured normal and endometriotic human endometrial stromal cells. In this experimental study, normal (n= 10) and endometriotic endometrial biopsies (n= 10) were taken in sterile condition. Stromal cells isolated and cultured in in DMEM/ F12 medium and treated with adiponectin concentrations (0, 10, 100, and 200 ng/ml) for 24 and 48 hours. NO assay was done...
Tipo: Journal article Palavras-chave: Adiponectin; Nitric oxide; Endometriosis; Stromal cell.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100053
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Differential expression of upstream stimulatory factor (USF) 2 variants in eutopic endometria from women with endometriosis: estradiol regulation Biol. Res.
Castro,Jazmin; Araya,Germán; Inostroza,Pamela; Hidalgo,Paulina; González-Ramos,Reinaldo; Sovino,Hugo; Angélica Boric,M.; Fuentes,Ariel; Cecilia Johnson,M..
BACKGROUND: Endometriosis, pro-inflammatory and invasive benign disease estrogen dependent, abnormally express in endometria the enzyme P450Arom, positively regulated by steroid factor-1 (SF-1). Our objective was to study the nuclear protein contents of upstream stimulating factor 2 (USF2a and USF2b), a positive regulator of SF-1, throughout the menstrual cycle in eutopic endometria from women with and without (control) endometriosis and the involvement of nuclear estrogen receptors (ER) and G-coupled protein estrogen receptor (GPER)-1 RESULTS: Upstream stimulating factor 2 protein contents were higher in mid (USF2b) and late (USF2a and USF2b) secretory phase in eutopic endometria from endometriosis than control (p < 0.05). In isolated control...
Tipo: Journal article Palavras-chave: USF2; GPER1; Endometriosis; Eutopic endometrium; Estrogen receptor; Estrogen receptor specific agonists; SF-1; P450Arom.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100056
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Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas BJMBR
Veiga-Castelli,L.C.; Rosa e Silva,J.C.; Meola,J.; Ferriani,R.A.; Yoshimoto,M.; Santos,S.A.; Squire,J.A.; Martelli,L..
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endometrioma; Endometriosis; Comparative genomic hybridization; Chromosomal imbalances.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014
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Histological Evaluation of the Induced Endometriosis in Rats, after Treatment with Dexamethasone International Journal of Morphology
Batista,Ana Paula Castor; Conceição,Ana Paula Maria da; Moraes,Eleonora de Figueiredo; Teixeira,Álvaro Aguiar Coelho; Wanderley Teixeira,Valéria.
The present research had the purpose to analyze morphologically the sites of endometrial implants on the external area of the musculature of the anterior abdominal wall of female rats, treated with dexamethasone. For so, these 15 albino female rats were used (Rattus norvegicus albinus), of the lineage Wistar with 90 days of age, that were submitted to the induction of the endometriosis, and divided in the following groups: Group I ­ induced female rats to endometriosis and evaluated after 21 days (control); Group II ­ induced female rats to endometriosis and after 21 days, treated with dexamethasone for 10 days; Group III - induced female rats to endometriosis, and after 21 days, treated with dexamethasone for 15 days. The dexamethasone was administered in...
Tipo: Journal article Palavras-chave: Endometriosis; Dexamethasone; Steriods; Hormones; Metaplasia.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500008
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Increased expression of ID2, PRELP and SMOC2 genes in patients with endometriosis BJMBR
Araujo,F.M.; Meola,J.; Rosa-e-Silva,J.C.; Paz,C.C.P.; Ferriani,R.A.; Nogueira,A.A..
Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Endometriosis; PRELP; SMOC2; ID2; Real-time PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700605
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Insulin-like growth factor II gene Apa I polymorphism is not associated with endometriosis susceptibility Genet. Mol. Biol.
Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Peng,Ching-Tien; Yeh,Lian-Shun; Lin,Cheng-Chieh.
Insulin-like growth factor II (IGF2) has been shown to play a role in abnormal cell growth and carcinogenesis. We investigated if the IGF2 gene Apa I polymorphism at exon 9 was associated with the susceptibility to endometriosis, analyzing 120 women with moderate/severe endometriosis and 103 controls. The genotype frequencies did not differ statistically between the endometriosis (aa = 25.4, ab = 57.4, bb = 17.2%) and control (aa = 20.8 ab = 52.8, bb = 26.4%) groups. The allele frequencies did not differ either: a = 54.1, b = 45.9% among women with endometriosis and a = 47.2, b = 52.8% in the control group. Therefore, no indication was found for an association of this polymorphism with endometriosis susceptibility.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endometriosis; Insulin-like growth factor; IGF-2.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200006
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Peritoneal and serum interleukin-18 levels are not increased in women with minimum or mild endometriosis BJMBR
Glitz,C.; Souza,C.A.; Rodini,G.P.; Genro,V.; Bilibio,J.P.; Senger,M.; Cunha-Filho,J.S..
Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 family. Endometriosis is strongly associated with sub-fertility, and affects about 15% of women of reproductive age. IL-18 may favor the progression of endometriosis. The objective of the present study was to determine the concentration of IL-18 in the serum and peritoneal fluid of infertile women with endometriosis. Forty infertile and 25 fertile women were screened in a teaching hospital. Thirty-four infertile patients with minimal or mild endometriosis and 22 fertile controls were enrolled in the study. The primary outcome was the estimate of IL-18 levels and the secondary outcome was the correlation between serum and peritoneal levels of IL-18. There were no differences between the two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokines; Endometriosis; IL-18; Peritoneal fluid; Infertility.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100007
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Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein BJMBR
Passos,E.P.; Brugnara,L.; Facin,A.C.; Riffel,A.; Lima,G.R.; Freitas,V.; Brandelli,A..
The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acrosome reaction; Endometriosis; Infertility; Peritoneal fluid; Sperm.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100009
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Prolactin and cortisol levels in women with endometriosis BJMBR
Lima,A.P.; Moura,M.D.; Rosa e Silva,A.A.M..
Endometriosis is a progressive estrogen-dependent disease affecting women during their reproductive years. The objective of the present study was to investigate whether endometriosis is associated with stress parameters. We determined cortisol and prolactin levels in serum, peritoneal and follicular fluid from infertile women with endometriosis and fertile women without the disease. The extent of the disease was staged according to the revised American Fertility Society classification (1997). Serum and peritoneal fluid were collected from 49 women aged 19 to 39 years undergoing laparoscopy. Eighteen women had stage I-II endometriosis and 10 had stage III-IV. Controls were 21 women undergoing laparoscopy for tubal sterilization. Follicular fluid was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endometriosis; Stress; Infertility; Prolactin; Cortisol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800015
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Reduction of blood nitric oxide levels is associated with clinical improvement of the chronic pelvic pain related to endometriosis BJMBR
Rocha,M.G.; Gomes,V.A.; Tanus-Santos,J.E.; Rosa-e-Silva,J.C.; Candido-dos-Reis,F.J.; Nogueira,A.A.; Poli-Neto,O.B..
The objective of this prospective study was to determine the plasma levels of nitric oxide (NO) in women with chronic pelvic pain secondary to endometriosis (n=24) and abdominal myofascial pain syndrome (n=16). NO levels were measured in plasma collected before and 1 month after treatment. Pretreatment NO levels (μM) were lower in healthy volunteers (47.0±12.7) than in women with myofascial pain (64.2±5.0, P=0.01) or endometriosis (99.5±12.9, P<0.0001). After treatment, plasma NO levels were reduced only in the endometriosis group (99.5±12.9 vs 61.6±5.9, P=0.002). A correlation between reduction of pain intensity and reduction of NO level was observed in the endometriosis group [correlation = 0.67 (95%CI = 0.35 to 0.85), P<0.0001]. Reduction of NO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic pelvic pain; Endometriosis; Abdominal myofascial pain syndrome; Nitric oxide; Inflammation; Central sensitization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400363
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The cuttable C-related genotype and allele for the E-cadherin 3’-UTR Pml I polymorphism are associated with higher susceptibility to endometriosis Genet. Mol. Biol.
Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Hsu,Chin-Moo; Lin,Cheng-Chieh; Tsai,Chang-Hai.
Epithelial cadherin (E-cadherin; CDH1) may influence pericellular proteolysis and intracellular signal transduction, which plays an essential part of tumor invasion. In our study we investigated the correlation between CDH1 gene polymorphism and endometriosis in two groups of pre-menopausal Taiwanese women, group 1 (n = 150) consisting of women with severe stage IV endometriosis and group 2 (n = 159) of women with no endometriosis. The polymerase chain reaction (PCR) was used to identify the cuttable (C) and uncuttable (T) polymorphism of the CDH1-Pml I gene (rs1801026) located on the 3’-untranslated region (3’-UTR) of chromosome 16 and compare the genotypes and allelic frequencies of this gene in both groups. We found that the genotype and allele...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Endometriosis; E-cadherin; Polymorphism; SNP.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500003
Registros recuperados: 11
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