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Registros recuperados: 8
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Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison Genet. Mol. Biol.
Christofoletti,Cintya A.; David,José Augusto O.; Fontanetti,Carmem S..
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Comet assay; Ethidium bromide; Silver staining; Tilapia.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024
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Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment BJMBR
Bondan,E.F.; Lallo,M.A.; Trigueiro,A.H.; Ribeiro,C.P.; Sinhorini,I.L.; Graça,D.L..
Schwann cell disturbance followed by segmental demyelination in the peripheral nervous system occurs in diabetic patients. Since Schwann cell and oligodendrocyte remyelination in the central nervous system is a well-known event in the ethidium bromide (EB) demyelinating model, the aim of this investigation was to determine the behavior of both cell types after local EB injection into the brainstem of streptozotocin diabetic rats. Adult male Wistar rats received a single intravenous injection of streptozotocin (50 mg/kg) and were submitted 10 days later to a single injection of 10 µL 0.1% (w/v) EB or 0.9% saline solution into the cisterna pontis. Ten microliters of 0.1% EB was also injected into non-diabetic rats. The animals were anesthetized and perfused...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Central nervous system; Diabetes mellitus; Ethidium bromide; Remyelination; Schwann cell; Oligodendrocyte.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500011
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Ethidium bromide-induced demyelination of the sciatic nerve of adult Wistar rats BJMBR
Riet-Correa,G.; Fernandes,C.G.; Pereira,L.A.V.; Graça,D.L..
Peripheral nerve ultrastructure was assessed after single or multiple local injections of the intercalating dye ethidium bromide. Thirty-four adult Wistar rats of both sexes were divided into five groups and maintained in a controlled environment with rat chow and water ad libitum throughout the experiment. The experimental animals were injected with 1 µl of 0.1% ethidium bromide in 0.9% saline into the central third of the left sciatic nerve 1 (group 1), 2 (group 2), 4 (group 3), 6 (group 4) or 8 (group 5) times. In groups 2 to 5 the injections were made at 28-day intervals. Control animals received the same amount of 0.9% saline. The animals were killed at different times after injection: group 1 at 7 days (2 rats) and 15 days (2 rats); for groups 2, 3,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Demyelination; Remyelination; Sciatic nerve; Ethidium bromide.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100014
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Genotypic and phenotypic detection of efflux pump in Rhodococcus equi BJM
Gressler,Letícia Trevisan; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da; Pötter,Luciana; Silveira,Bibiana Petri da; Sangioni,Luis Antônio; Botton,Sônia de Avila.
The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethidium bromide; Efflux pump; Multidrug resistance; Rhodococcus equi; VapA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200040
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Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide Genet. Mol. Biol.
Becker,Tânia C.A.; Chiuchetta,Simone J.R.; Baptista,Francielle; Castro-Prado,Marialba A.A. de.
Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antineoplastic drug; Aspergillus nidulans; Ethidium bromide; Intercalating agent; Mitotic crossing over.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026
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Inflammatory response of the spinal cord to multiple episodes of blood-brain barrier disruption and toxic demyelination in Wistar rats BJMBR
Fernandes,C.G.; Graça,D.L.; Pereira,L.A.V.D..
Multiple episodes of blood-brain barrier disruption were induced by sequential intraspinal injections of ethidium bromide. In addition to the barrier disruption, there was toxic demyelination and exposure of myelin components to the immune system. Twenty-seven 3-month-old Wistar rats received 2, 3 or 4 injections of 1 µl of either 0.1% ethidium bromide in normal saline (19 rats) or 0.9% saline (8 rats) at different levels of the spinal cord. The time intervals between the injections ranged from 28 to 42 days. Ten days after the last injection, all rats were perfused with 2.5% glutaraldehyde. The spinal sections were evaluated macroscopically and by light and transmission electron microscopy. All the lesions demonstrated a mononuclear phagocytic infiltrate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lymphocytes; Multiple sclerosis; Central nervous system; Blood-brain barrier; Ethidium bromide.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700008
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Prodigiosin found in Serratia marcescens y2 initiates phototoxicity in the cytomembrane Electron. J. Biotechnol.
Wang,Fei; Luo,Hailan; Song,Guohua; Liu,Chang; Wang,Jianguo; Xu,Jingliang; Su,Xiuhong; Ma,Xu Yuan.
Background: Light can be absorbed by bacterial pigment and affects its growth. Prodigiosin is a red pigment found in various bacterial species. The purpose of this study was to investigate the impacts of light on prodigiosin production, biomass formation, and membrane integrity of Serratia marcescens y2. Results: S. marcescens y2 grew better and produced more intracellular prodigiosin in darkness than in illumination. The pigment leakage ratio from cells was detected more in light than in darkness conditions. Ethidium bromide uptake assay could visually prove the prodigiosin-related loss of membrane integrity under illumination. A higher concentration of malondialdehyde (MDA) was detected in light-treated culture than in darkness. Tests of different light...
Tipo: Journal article Palavras-chave: Ethidium bromide; Homogeneous light; Malondialdehyde; Red pigment.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400007
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ผลของการเสริม Ethidium bromide หรือ CuSO4 ในปฏิกิริยา Loop-mediated isothermal amplification (LAMP) ต่อการเพิ่มความแม่นยำในการคัดเพศตัวอ่อนโคเนื้อ Thai Agricultural
Saruttiwong Boonkong; Chainarong Navanukraw; Tossapol Moonmanee; Jiratti Thammasiri; Vilaivan Khanthusaeng; Aree Kraisoon.
The study was conducted to determine effect of EB or CS supplements in LAMP reaction on greater accuracy of sexing cattle embryos. Bovine ovaries were collected from the slaughterhouse, oocytes were aspirated from follicles then were evaluated morphology. Oocytes were cultured in TCM-199 at 38.5 ºC 24 h. For in vitro fertilization (IVF), oocytes were co-incubated with spermatozoa at 38.5 ºC 24 h. Fertilization rate was evaluated and then the embryos were in vitro cultured until morula stage. Then, embryo samples were frozen and stored at -196 ºC. Frozen embryos (n=35) were randomly extracted for total cellular DNA and amplified by PCR method. DNA samples were stored at -20 ºC. Sexing was determined by modification of LAMP method with either 1mM EB or 1M CS...
Tipo: PhysicalObject Palavras-chave: Cattle; Bovine; Oocyte; LAMP reaction; Loop-mediated isothermal amplificatio; Ethidium bromide; EB; CuSO4; CS; DNA; Sexing embryo; In vitro fertilization; IVF; โคเนื้อ; โอโอไซต์; ลักษณะทางสัณฐานวิทยา; การคัดเพศตัวอ่อน; เทคนิคการคัดเพศ; ดีเอ็นเอ; ความแม่นยำ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5813
Registros recuperados: 8
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