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Registros recuperados: 35
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Alternative mitochondrial functions in cell physiopathology: beyond ATP production BJMBR
Kowaltowski,A.J..
It is well known that mitochondria are the main site for ATP generation within most tissues. However, mitochondria also participate in a surprising number of alternative activities, including intracellular Ca2+ regulation, thermogenesis and the control of apoptosis. In addition, mitochondria are the main cellular generators of reactive oxygen species, and may trigger necrotic cell death under conditions of oxidative stress. This review concentrates on these alternative mitochondrial functions, and their role in cell physiopathology.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondrial permeability transition; Uncoupling proteins; Alternative oxidase; Cardioprotection; Free radicals; Aging.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200014
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Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats IPB - Escola Superior Agrária
Ramirez-Tortosa, Carmen; Cabrita, Luís; Andersen, Øyvind M.; Gardner, P.T.; Morrice, P.C.; Wood, S.G.; Duthie, S.J.; Collins, A.R.; Garry, G.G..
Anthocyanins are secondary plant metabolites responsible for the blue, purple, and red color of many plant tissues. The phenolic structure of anthocyanins conveys marked antioxidant activity in model systems via donation of electrons or hydrogen atoms from hydroxyl moieties to free radicals. Dietary intakes of anthocyanins may exceed 200 mg/day, however, little is known about their antioxidant potency in vivo. Consequently, the aim of this study was to establish whether anthocyanins could act as putative antioxidant micronutrients. Rats were maintained on vitamin E-deficient diets for 12 weeks in order to enhance susceptibility to oxidative damage and then repleted with rations containing a highly purified anthocyanin-rich extract at a concentration of 1...
Tipo: Article Palavras-chave: Anthocyanins; Polyphenols; Lipid peroxides; DNA damage; Antioxidants; Free radicals.
Ano: 2001 URL: http://hdl.handle.net/10198/3368
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Antioxidant activities of fractions from longan pericarps Ciênc. Tecnol. Aliment.
Yang,Xuena; Yan,Fen; Huang,Shangrong; Fu,Caili.
The antioxidant activities of ethanolic crude extract (LPCE) and its four different solvent sub-fractions (namely, diethyl ether fraction (LPDF), ethyl acetate fraction (LPEF), n-butyl alcohol fraction (LPBF) and residue fraction (LPR)) from longan pericarps were investigated employing various systems including 2,2-diphenyl-1-picrylhydrazyl (DPPH)/ 2,2'-amino-di(2-ethyl-benzothiazoline sulphonic acid-6)ammonium salt (ABTS)/hydroxyl radical scavenging activity, total phenolic content and reducing power. Each extract showed concentration-dependent antioxidant activity. LPEF showed the highest scavenging activity against DPPH, ABTS and hydroxyl radicals with EC50 values of 0.506, 0.228 and 4.489 mg/mL, respectively. LPEF showed the highest reducing power with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Longan pericarp; Extract; Free radicals; Antioxidant activity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200018
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Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate Agronomy
Guedes, Mayara Neves Santos; Pio, Rafael; Maro, Luana Aparecida Castilho; Lage, Fabíola Fonseca; Abreu, Celeste Maria Patto de; Saczk, Adelir Aparecida.
Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 total phenols; Free radicals; HPLC; Rubus spp. Fitotecnia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/28413
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Antioxidant activity of extract from Polygonum cuspidatum Biol. Res.
HSU,CHIN-YUAN; CHAN,YU-PEI; CHANG,JELI.
Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zuce has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value oí Polygonum cuspidatum extract is 110 µg/ml in free radical scavenging assays, 3.2 µg/ml in superoxide radical scavenging...
Tipo: Journal article Palavras-chave: Antioxidant activity; Free radicals; Phenolics; Lipid peroxidation; DNA damage Polygonum cuspidatum.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100002
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Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal Biol. Res.
Paudel,Babita; Datta Bhattarai,Hari; Prasad Pandey,Durga; Seoun Hur,Jae; Gyu Hong,Soon; Kim,Il-Chan; Han Yim,Joung.
A total of twenty four lichen species belonging to six families were collected from mountainous region of Nepal. The methanol extracts of each species were tested for antimicrobial and antioxidant activitiesin vitro. It was found that extracts of twenty one lichen species were active againstB. subtilis and seven species were active againstS. aureus. Similarly, in DPPH assay, three speciesPeltigera sp.,Cladonia sp., andCanoparmelia sp. showed comparable activity with commercial standard, BHA. In ABTS+ assay, extracts ofParmoterma sp.,Ramalina sp.,Peltigera sp. andCladonia sp. showed stronger activity than ascorbic acid. The observed data after comparison with previously published reports indicated that the high altitude lichens contain stronger antioxidant...
Tipo: Journal article Palavras-chave: Antibacterial; Antioxidant; Nepal lichens; Free radicals.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400010
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Antioxidant properties of natural compounds used in popular medicine for gastric ulcers BJMBR
Repetto,M.G.; Llesuy,S.F..
There is evidence concerning the participation of reactive oxygen species in the etiology and physiopathology of human diseases, such as neurodegenerative disorders, inflammation, viral infections, autoimmune pathologies, and digestive system disorders such as gastrointestinal inflammation and gastric ulcer. The role of these reactive oxygen species in several diseases and the potential antioxidant protective effect of natural compounds on affected tissues are topics of high current interest. To consider a natural compound or a drug as an antioxidant substance it is necessary to investigate its antioxidant properties in vitro and then to evaluate its antioxidant functions in biological systems. In this review article, we shall consider the role of natural...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Free radicals; Natural antioxidants; Gastric ulcers; Artemisia douglasiana Besser; Dehydroleucodine.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500003
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Application of various antioxidants in the treatment of influenza BJMBR
Dolganova,A.; Sharonov,B.P..
We determined the effect of the antioxidants superoxide dismutase, desferrioxamine and allopurinol on the survival of male CBA mice infected intranasally with 2-5 LD50 lung influenza virus A/Aichi/2/68. Survival for at least 20 days was observed for 45% of the mice that received 1000 U/day superoxide dismutase prepared from red blood cells on days 5, 6, 7 and 8 after infection, and 75% survival was observed for mice that received the same dose on days 4, 5, 6, 7 and 8. Desferrioxamine, 25 mg/kg per day and 100 mg/kg per day injected subcutaneously, resulted in survival rates of 5 and 0%, respectively, compared to 10% survival observed for saline-injected controls. Allopurinol at doses of 5 to 50 mg/kg per day had no effect on mouse survival. These data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Free radicals; Influenza; Antioxidant; Desferrioxamine; Superoxide dismutase; Allopurinol.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100012
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Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma Biol. Res.
LOTITO,SILVINA B; FRAGA,CESAR G.
We evaluated the interaction between ascorbic acid (AA) and (+)-catechin (CTCH) in potassium phosphate solution, pH 7.4 (PPS) and in human plasma. In both systems, the oxidation was started by adding 2,2’-azobis-(2-amidinopropane) clorhidrate (AAPH). The concentrations of AA and CTCH were determined by HPLC using electrochemical detection. In PPS, CTCH was oxidized by AAPH (50 muM), in either the absence or presence of different initial concentrations of AA (25-200 muM). In the presence of AA, CTCH depletion was delayed, an effect that was dependent upon the initial concentration of AA. When 100 muM AA was added after the oxidation had begun, CTCH depletion was arrested for 30 min. The kinetics of AA oxidation by AAPH was also characterized in PPS. AA (100...
Tipo: Journal article Palavras-chave: Catechins; Antioxidants; Free radicals; Procyanidins.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200015
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Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride Biol. Res.
SOUZA,RAPHAEL S S; ALMEIDA,MARCELA C; MANOEL,CRISTIANO V; SANTOS-FILHO,SEBASTIÃO D; FONSECA,ADENILSON S; FILHO,MARIO BERNARDO.
Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a) SnC12 (25mg/ml), (b)Salix alba extract(11.6mg/ml) and (c)SnC12(25mg/ml) + Salix alba extract (11.6mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60...
Tipo: Journal article Palavras-chave: Escherichia coli; Free radicals; Salix alba; Stannous chloride.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008
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Determinação quantitativa da atividade antioxidante de extratos brutos de microrganismos pelo método de captura de radical livre DPPH. Infoteca-e
SANTOS, S. N.; CASTANHA, R. F.; HABER, L. L.; MARQUES, M. O. M.; SCRAMIN, S.; MELO, I. S. de.
O escopo deste trabalho é a disponibilização da metodologia científica utilizada na determinação quantitativa da atividade antioxidante de extratos livres DPPH, baseadas nas adaptações realizadas no Laboratório de Microbiologia Ambiental em parceria com o Laboratório de Produtos Naturais, ambos da Embrapa Meio Ambiente.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Radical livre; Microrganismo; Análise microbiológica; Análise bacteriológica; Análise de laboratório; Método; Laboratory techniques; Free radicals.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/898412
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Effect of fermentation time on phenolic content and antioxidant potential in Cupuassu (Theobroma grandiflorum (Willd. ex Spreng.) K.Schum.) beans Acta Agron. (Palmira)
Cuellar-Álvarez,Laura; Cuellar-Álvarez,Natalia; Galeano-García,Paula; Suárez-Salazar,Juan Carlos.
Abstract Cupuassu (Theobroma grandiflorum (Willd. ex Spreng.) K.Schum.) is an evergreen tree in the family Malvaceae, with nutritional qualities of interest in the food and cosmetic industry. It is necessary for its processing, in addition to other processes, to perform a fermentation, affecting its chemical composition. Therefore, the effect of fermentation time on the phenolic content and antioxidant activity of Cupuassu (T. grandiflorum) beans, was determined. During this process, the chemical properties of the beans and the phenolic content were evaluated every two days; also quantifying the secondary metabolites Catechin, Epicatechin, Theobromine and Caffeine by high performance liquid chromatography (HPLC). The antioxidant activity was analyzed using...
Tipo: Journal article Palavras-chave: Bromatological analysis; Phenols; Fermentation; Free radicals; Análisis bromatológico; Fenoles; Fermentación; Radicales libres.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400473
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Effect of freezing and processing technologies on the antioxidant capacity of fruit pulp and jelly BABT
Bof,Cristine Maso Jeusti; Fontana,Roselei Claudete; Piemolini-Barreto,Luciani Tatsch; Sandri,Ivana Greice.
The effect of freezing and processing technology on the antioxidant capacity of grape (Vitis vinifera), apple (Malus domestica), strawberry (Fragaria x Anassa), pear (Pyrus communis L.), guava (Psidium guajava L.), and fig (Ficus carica L.) was evaluated for 90 days. Under a storage temperature of -15 º C, there was no significant difference in the antioxidant capacity of grape and fig pulp, and a higher antioxidant capacity was found for guava pulp (27 µmol/g). While the technological processing did not affect the antioxidant capacity of pear and apple jellies, all other jellies studied showed a reduced antioxidant capacity. The processing reduced the antioxidant capacity of grapes in 45%. Among the fruit products, the highest antioxidant activities were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Functional foods; Pulps; Jellies; Free radicals; Storage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100014
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Effect of trolox C on cardiac contracture induced by hydrogen peroxide BJMBR
Belló-Klein,A.; Oliveira,A.R.; Miranda,M.F.S.; Irigoyen,M.C.; Homem-de-Bittencourt Jr.,P.I.; Llesuy,S.; Belló,A.A..
Hydrogen peroxide (H2O2) perfused into the aorta of the isolated rat heart induces a positive inotropic effect, with cardiac arrhythmia such as extrasystolic potentiation or cardiac contractures, depending on the dose. The last effect is similar to the "stone heart" observed in reperfusion injury and may be ascribed to lipoperoxidation (LPO) of the membrane lipids, to protein damage, to reduction of the ATP level, to enzymatic alterations and to cardioactive compounds liberated by LPO. These effects may result in calcium overload of the cardiac fibers and contracture ("stone heart"). Hearts from male Wistar rats (300-350 g) were perfused at 31oC with Tyrode, 0.2 mM trolox C, 256 mM H2O2 or trolox C + H2O2. Cardiac contractures (baseline elevation of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trolox C; "stone heart"; Free radicals; Hydrogen peroxide.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100013
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Enhanced response of the fricke solution doped with hematoporphyrin under X-rays irradiation BABT
Austerlitz,Carlos; Souza,Vivianne Lúcia Bormann de; Campos,Diana Maria Tavares; Kurachi,Cristina; Bagnato,Vanderley; Sibata,Cláudio.
The vials filled with Fricke solutions were doped with increasing concentrations of Photogem®, used in photodynamic therapy. These vials were then irradiated with low-energy X-rays with doses ranging from 5 to 20 Gy. The conventional Fricke solution was also irradiated with the same doses. The concentration of ferric ions for the Fricke and doped-Fricke irradiated solutions were measured in a spectrophotometer at 220 to 340 nm. The results showed that there was an enhancement in the response of the doped-Fricke solution, which was proportional to the concentration of the photosensitizer. The use of such procedure for studying the radiosensitizing property of photosensitizers based on the production of free radicals is also discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Photodynamic therapy; Hematoporphyrin; Fricke dosimeter; Free radicals.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200006
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Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice Acta Amazonica
CUNHA,Ana Paula Simões da; BALDISSERA,Luana; PEREIRA,Débora Linsbinski; ALBIERO,Lucineia Reuse; CASTOLDI,Lindsey; SINHORIN,Adilson Paulo; SINHORIN,Valéria Dornelles Gindri.
ABSTRACT Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid tumor; Oxidative stress; Free radicals; Copaiba; Antineoplasic.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100041
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Free radical scavenging capacity and cytotoxic and antiproliferative effects of Vaccinium meridionale Sw. agains colon cancer cell lines Plantas Medicinales
Maldonado-Celis,Maria E; Arango-Varela,Sandra Sulay; Rojano,Benjamín Alberto.
Introduction: Vaccinium meridionale Swartz, of the family Ericaceae, is commonly known as mortiño or agraz. The plant is considered a functional food, with a content of anthocyanins and antioxidants similar to or greater than that reported for other Vaccinium species. However, little is known about its nutraceutical and medicinal properties. Objectives: determine the antioxidant activity and cytotoxic and antiproliferative effect of mortiño fruit aqueous extract against colon adenocarcinoma cells (SW480) and their derived metastatic cells (SW620). Methods: total phenols and anthocyanins were determined by the Folin-Ciocalteu method. Caffeoyl derivatives were determined by HPLC-DAD. Antioxidant activity was analyzed as the ability to scavenge reactive...
Tipo: Journal article Palavras-chave: Cytotoxic; Antiproliferative; Free radicals; Vaccinium meridionale; Colon cancer.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962014000200006
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Ginseng administration protects skeletal muscle from oxidative stress induced by acute exercise in rats BJMBR
Voces,J.; Cabral de Oliveira,A.C.; Prieto,J.G.; Vila,L.; Perez,A.C.; Duarte,I.D.G.; Alvarez,A.I..
Enzymatic activity was analyzed in the soleus, gastrocnemius (red and white) and plantaris muscles of acutely exercised rats after long-term administration of Panax ginseng extract in order to evaluate the protective role of ginseng against skeletal muscle oxidation. Ginseng extract (3, 10, 100, or 500 mg/kg) was administered orally for three months to male Wistar rats weighing 200 ± 50 g before exercise and to non-exercised rats (N = 8/group). The results showed a membrane stabilizing capacity of the extract since mitochondrial function measured on the basis of citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities was reduced, on average, by 20% (P < 0.05) after exercise but the activities remained unchanged in animals treated with a ginseng...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Panax ginseng; Exercise; Free radicals; Lipid peroxidation; Glutathione status; Mitochondria.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200012
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Glutathione and the redox control system trypanothione/trypanothione reductase are involved in the protection of Leishmania spp. against nitrosothiol-induced cytotoxicity BJMBR
Romão,P.R.T.; Tovar,J.; Fonseca,S.G.; Moraes,R.H.; Cruz,A.K.; Hothersall,J.S.; Noronha-Dutra,A.A.; Ferreira,S.H.; Cunha,F.Q..
Glutathione is the major intracellular antioxidant thiol protecting mammalian cells against oxidative stress induced by oxygen- and nitrogen-derived reactive species. In trypanosomes and leishmanias, trypanothione plays a central role in parasite protection against mammalian host defence systems by recycling trypanothione disulphide by the enzyme trypanothione reductase. Although Kinetoplastida parasites lack glutathione reductase, they maintain significant levels of glutathione. The aim of this study was to use Leishmania donovani trypanothione reductase gene mutant clones and different Leishmania species to examine the role of these two individual thiol systems in the protection mechanism against S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania; Glutathione; Trypanothione; Trypanothione reductase; Nitric oxide; Free radicals.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300006
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High susceptibility of activated lymphocytes to oxidative stress-induced cell death Anais da ABC (AABC)
Degasperi,Giovanna R.; Castilho,Roger F.; Vercesi,Anibal E..
The present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell death; Free radicals; Immune response; Lymphopenia; Mitochondrial permeability transition; Spleen lymphocyte.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100009
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