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A high-fat diet decreases GABA concentration in the frontal cortex and hippocampus of rats Biol. Res.
Sandoval-Salazar,Cuauhtemoc; Ramírez-Emiliano,Joel; Trejo-Bahena,Aurora; Oviedo-Solís,Cecilia I.; Solís-Ortiz,Martha Silvia.
BACKGROUND: It has been proposed that the γ-aminobutyric acid (GABA) plays a key role in the regulation of food intake and body weight by controlling the excitability, plasticity and the synchronization of neuronal activity in the frontal cortex (FC). It has been also proposed that the high-fat diet (HFD) could disturb the metabolism of glutamate and consequently the GABA levels, but the mechanism is not yet clearly understood. Therefore, the aim of this study was to investigate the effect of a HFD on the GABA levels in the FC and hippocampus of rats RESULTS: The HFD significantly increased weight gain and blood glucose levels, whereas decreased the GABA levels in the FC and hippocampus compared with standard diet-fed rats CONCLUSIONS: HFD...
Tipo: Journal article Palavras-chave: Obesity; GABA; Frontal cortex; Hippocampus; High-fat diet.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100015
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Accumulation of gamma-aminobutyric acid (GABA) in non-waxy and waxy rice germ during water soaking Thai Agricultural
Patcharee Tungtrakul; Warunee Varanyanond; Vipa Surojanametakul; Ladda Wattanasiritham.
Rice germ contains protein, vitamins, minerals, dietary fiber and gamma-aminobutyric acid (GABA). GABA has been proved to be effective for lowering the blood pressure of human being. It has neurotransmission functions and tranquilizer effects. determination of GABA in rice germ and brown rice were investigated by using low-amylose, high-amylose and waxy rice cultivars. Percentage of germ weight showed no relation to the GABA content. The amount and patterns of the GABA accumulation varied depending on the cultivar. GABA content in most of rice varieties increased during incubation. The accumulation of gamma-aminobutyric acid (GABA) in non-waxy and waxy rice germ during water soaking were determined by using 14 different cultivars. Water soaking can enrich...
Tipo: PhysicalObject Palavras-chave: Accumulation; GABA; Rice germ; Gamma-aminobutyric acid; Waxy rice; ข้าวเจ้า; ข้าวเหนียว; คัพภะ; กรดแกมมา-แอมิโนบิวเทริก; การแช่น้ำ.
Ano: 2006 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5140
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Cardiovascular responses to microinjections of GABA or anesthetics into the rostral ventrolateral medulla of conscious and anesthetized rats BJMBR
Lacerda,J.E.C.; Campos,R.R.; Araujo,G.C.; Andreatta-Van Leyen,S.; Lopes,O.U.; Guertzenstein,P.G..
The rostral ventrolateral medulla (RVLM) contains neurons involved in tonic and reflex control of arterial pressure. We describe the effects of gamma-aminobutyric acid (GABA) and anesthetics injected into the RVLM of conscious and urethane (1.2 g/kg, iv) anesthetized Wistar rats (300-350 g). In conscious rats, bilateral microinjection of GABA (50 nmol/200 nl) induced a small but significant decrease in blood pressure (from 130 ± 3.6 to 110 ± 5.6 mmHg, N = 7). A similar response was observed with sodium pentobarbital microinjection (24 nmol/200 nl). However, in the same animals, the fall in blood pressure induced by GABA (from 121 ± 8.9 to 76 ± 8.8 mmHg, N = 7) or pentobarbital (from 118 ± 4.5 to 57 ± 11.3 mmHg, N = 6) was significantly increased after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vasomotor tone; Ventrolateral medulla; RVLM; GABA; Conscious rats.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900019
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EFECTO DE LA ADMINISTRACIÓN INTRACEREBRAL DE MK-801 Y (-) NICOTINA EN LAS CONCENTRACIONES EXTRACELULARES DE GLU Y GABA EN EL NÚCLEO PEDUNCULOPONTINO DE RATAS Acta biol.Colomb.
BLANCO LEZCANO,LISETTE; LORIGADOS PEDRE,LOURDES DEL CARMEN; GONZÁLEZ FRAGUELA,MARÍA ELENA; SERRANO SÁNCHEZ,TERESA; PAVÓN FUENTES,NANCY; BLANCO LEZCANO,VIVIAN; FRANCIS TURNER,LILIANA.
Aunque la manipulación farmacológica de los sistemas glutamatérgico y colinérgico se ha tratado en modelos experimentales de enfermedad de Parkinson (EP), pocos autores han realizado estudios de esta temática a nivel del núcleo pedunculopontino (NPP). El presente trabajo aborda los cambios en las concentraciones extracelulares (CE) de glutamato (Glu) y ácido δ-amino butírico (GABA) en el NPP de ratas hemiparkinsonizadas por inyección de 6-hidroxidopamina (6-OHDA) y sometidas a infusión local de MK-801 (10 µmol/L) o (-) nicotina (10 mM). La infusión se realizó mediante microdiálisis cerebral y la determinación de CE de neurotransmisores se realizó a través de cromatografía líquida de alta resolución acoplada a detección de fluorescencia. La...
Tipo: Journal article Palavras-chave: MK-801; Nicotina; Núcleo pedunculopontino; Glutamato; GABA.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000100004
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Effect of the GABA B agonist baclofen on dipyrone-induced delayed gastric emptying in rats BJMBR
Collares,E.F.; Vinagre,A.M..
Dipyrone administered intravenously (iv) or intracerebroventricularly (icv) delays gastric emptying (GE) in rats. Gamma-aminobutyric acid (GABA) is the most potent inhibitory neurotransmitter of the central nervous system. The objective of the present study was to determine the effect of icv baclofen, a GABA B receptor agonist, on delayed GE induced by dipyrone. Adult male Wistar rats received a saline test meal containing phenol red as a marker. GE was indirectly evaluated by determining the percent of gastric retention (%GR) of the meal 10 min after orogastric administration. In the first experiment, the animals were injected iv with vehicle (Civ) or 80 mg/kg (240 µmol/kg) dipyrone (Dp iv), followed by icv injection of 10 µl vehicle (bac0), or 0.5...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gastric emptying; Dipyrone; GABA; GABA B receptors; Baclofen.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100015
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Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress Rev. Bras. Ciênc. Avic.
Xie,XZ; Liang,C; Li,MH; Chen,Z.
ABSTRACT Under routine feeding conditions, 1-day-old male chicks were randomly divided into control group (CK), heat-stress group (HS), and GABA + heat stress group (GABA+HS). The thymus contents of IL-1, IL-2, TGF-β1, IFN-γ, GH and HSP70 were determined by enzyme-linked immunosorbent assays. The results showed that: (1) IL-1 and TGF-β1 contents of HS group were significantly lower than those of the CK group (p<0.05), and those of the GABA+HS group were significantly higher relative to the HS group (p<0.05); (2) IL-2 and IFN-γ contents of the HS group were significantly higher than those of the CK group (p<0.05), and those of the GABA+HS group were significantly lower relative to the HS group (p<0.05);(3) the thymus GH content of all three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat stress; GABA; Cytokines; Thymus; Chicks.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100143
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Frequent occurrence of nicotinic acetylcholine receptors in GABAergic neurons of the chick visual system BJMBR
Torrão,A.S.; Britto,L.R.G..
Double-labeling immunohistochemical methods were used to investigate the occurrence of the alpha8 and alpha5 nicotinic receptor subunits in presumptive GABAergic neurons of the chick nervous system. Nicotinic receptor immunoreactivity was often found in cells exhibiting GABA-like immunoreactivity, especially in the visual system. The alpha8 subunit appeared to be present in presumptive GABAergic cells of the ventral lateral geniculate nucleus, nucleus of the basal optic root of the accessory optic system, and the optic tectum, among several other structures. The alpha5 subunit was also found in GABA-positive neurons, as observed in the lentiform nucleus of the mesencephalon and other pretectal nuclei. The numbers of alpha8- and alpha5-positive neurons that...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Acetylcholine; GABA; Neurotransmitters; Nicotinic receptors; Visual system.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000013
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In vivo photorelease of GABA in the mouse cortex BJMBR
Lopes-dos-Santos,V.; Campi,J.; Filevich,O.; Ribeiro,S.; Etchenique,R..
Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to activate or deactivate specific neuronal populations within a single stimulation site. This problem can be avoided by pharmacological methods based on the administration of receptor ligands able to cause specific changes in neuronal activity. However, intracerebral injections of neuroactive molecules inherently confound the dynamics of drug diffusion with receptor activation. Caged compounds have been proposed to circumvent this problem, for spatially and temporally...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caged compounds; Neural stimulation; GABA; Cerebral cortex; Photorelease.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700012
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Inhibition of L-glutamate and GABA synaptosome uptake by crotoxin, the major neurotoxin from Crotalus durissus terrificus venom J. Venom. Anim. Toxins incl. Trop. Dis.
Cecchini,A.L.; Soares,A.M.; Giglio,J.R.; Amara,S.; Arantes,E.C..
This paper describes a brief study on the crotoxin mechanism of action, regarding the transport of GABA and L-glutamate in rats cortico-cerebral synaptosomes and in heterologous systems, such as COS-7 cells expressing gabaergic transporters, and C6 glioma cells and Xenopus oocytes expressing glutamatergic transporters. Crotoxin concentrations over 1 µM caused an inhibitory effect of ³H-L-glutamate and ³H-GABA, and reversibly inhibited L-glutamate uptake by C6 glioma cells. When COS-7 cells were assayed, no inhibition of the ³H-GABA transport could be evidenced. Crotoxin kept its inhibitory effect on neurotransmitters uptake even when Ca2+ ions were removed from the medium, therefore, independently of its PLA2 activity. In addition, high concentrations (2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotoxin; L-glutamate; GABA; Crotalus durissus terrificus.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300006
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Localization of Vesicular Inhibitory Amino Acid Transporter (VIAAT) in the Submandibular Salivary Gland Throughout the Postnatal Development of Mice International Journal of Morphology
Toomsan,Yanyong; Chomphoo,Surang; Pakkarato,Sawetree; Watanabe,Masahiko; Kondo,Hisatake; Hipkaeo,Wiphawi.
According to recent studies, it is highly possible that the occurrence of vesicular inhibitory amino acid transporter (VIAAT) is a good marker of GABA-signaling not only in the brain, but also in extra-brain tissue cells containing GABA and GAD. In view of this, the present study was attempted to localize VIAAT-immunoreactivity in the submandibular gland of mice. In the present study, the submandibular glands of male mice at various postnatal developmental stages were examined for detailed localization of VIAAT-immunoreactivity in immunohistochemistry at light microscopic level. The immunoreactivity for VIAAT was localized in epithelial cells of proximal and distal excretory ducts with the striated portion more intensely immunopositive at young postnatal...
Tipo: Journal article Palavras-chave: VIATT-immunoreactivity; GABA; Salivary glands; Arteriolar smooth muscle.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100019
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Moxidectin interference on motor activity of rats BABT
Rodrigues-Alves,Patrícia de Sá e Benevides; Flório,Jorge Camilo; Lebrun,Ivo; Bernardi,Maria Martha; Spinosa,Helenice de Souza.
The present study investigated the effects of t moxidectin (MXD) in some parameters of rat motor function and neurochemical. The general activity in the open field and the motor coordination in the wooden beam were employed to evaluate the MXD effects. The results showed that, in the open field, even at high doses (2.0 and 20.0 mg/kg), the MXD did not alter the locomotion and the rearing frequencies. However, MXD was able to impair the motor coordination of the animals at wooden beam. Neurochemical studies of striatal GABA and dopamine neurotransmitters showed a reduced levels of dopamine and its metabolite, homovanillic acid, without interference on striatal GABA levels. Since GABAergic receptor stimulation had an inhibitory effect on dopaminergic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Moxidectin; GABA; Motor coordination; Rat; Open field; Wooden beam.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400012
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Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil BJMBR
Heldwein,C.G.; Silva,L.L.; Reckziegel,P.; Barros,F.M.C.; Bürger,M.E.; Baldisserotto,B.; Mallmann,C.A.; Schmidt,D.; Caron,B.O.; Heinzmann,B.M..
The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA A receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: False-melissa; Silver catfish; Benzodiazepines; Flumazenil; GABA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500009
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Reduced hippocampal GABAergic function in Wistar audiogenic rats BJMBR
Drumond,L.E.; Kushmerick,C.; Guidine,P.A.M.; Doretto,M.C.; Moraes,M.F.D.; Massensini,A.R..
Epilepsy is a neurological disorder associated with excitatory and inhibitory imbalance within the underlying neural network. This study evaluated inhibitory γ-amino-butyric acid (GABA)ergic modulation in the CA1 region of the hippocampus of male Wistar rats and Wistar audiogenic rats (aged 90 ± 3 days), a strain of inbred animals susceptible to audiogenic seizures. Field excitatory postsynaptic potentials and population spike complexes in response to Schaffer collateral fiber stimulation were recorded in hippocampal slices before and during application of picrotoxin (50 µM, 60 min), a GABA A antagonist, and the size of the population spike was quantified by measuring its amplitude and slope. In control audiogenic-resistant Wistar rats (N = 9), picrotoxin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epilepsy; Extracellular recording; GABA; Hippocampus; Wistar audiogenic rats; Picrotoxin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000011
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Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak) Ciênc. Tecnol. Aliment.
TANAMOOL,Varavut; HONGSACHART,Piyorot; SOEMPHOL,Wichai.
Abstract This study aimed to evaluate the capability of lactic acid bacteria (LAB) isolated from different kinds of fermented fish products (Plaa-som) on the production of γ-aminobutyric acid (GABA). Among them, isolate L10-11, identified as Lactobacillus plantarum, offered the highest GABA production and was selected for further study. The highest production of GABA was obtained within 48h from the de Man, Rogosa and Sharpe (MRS) medium, having a pH range of 5-6. Increasing the monosodium glutamate (MSG) concentration resulted in a higher accumulation of GABA and reached the highest concentration (15.74 g/L) using 4% (w/v) MSG, while high residual MSG was also observed in accordance with concentration increase. On the other hand, the addition of NaCl in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; GABA; Fermented fish; Plaa-som; Som-pak.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028101
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Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen) BJMBR
Silva,L.L.; Garlet,Q.I.; Benovit,S.C.; Dolci,G.; Mallmann,C.A.; Bürger,M.E.; Baldisserotto,B.; Longhi,S.J.; Heinzmann,B.M..
This study evaluated the sedative and anesthetic effects of the essential oils (EO) of Hyptis mutabilis (Rich.) Briq. and their isolated components on silver catfish (Rhamdia quelen). Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+)-1-terpinen-4-ol and (-)-globulol] showed different activity from that detected for the leaf EO in proportional concentrations and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silver catfish; (+)-1-Terpinen-4-ol; (-)-Globulol; Sedation; GABA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900771
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The influence of stress on fear memory processes BJMBR
Martijena,I.D.; Molina,V.A..
It is well recognized that stressful experiences promote robust emotional memories, which are well remembered. The amygdaloid complex, principally the basolateral complex (BLA), plays a pivotal role in fear memory and in the modulation of stress-induced emotional responses. A large number of reports have revealed that GABAergic interneurons provide a powerful inhibitory control of the activity of projecting glutamatergic neurons in the BLA. Indeed, a reduced GABAergic control in the BLA is essential for the stress-induced influence on the emergence of associative fear memory and on the generation of long-term potentiation (LTP) in BLA neurons. The extracellular signal-regulated kinase (ERK) subfamily of the mitogen-activated protein kinase (MAPK) signaling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stress; Amygdala; GABA; Memory; Fear.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400004
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ความสัมพันธ์ของฤทธิ์ต้านอนุมูลอิสระกับปริมาณ Tocopherol และ gamma aminobutyric acid (GABA) ในข้าวกล้องและข้าวกล้องงอก Thai Agricultural
Pakinee Akkaravessapong; Suganya Wongpornchai; Sunanta Wongpiyachon; Kitsada Pitija; Angsutorn Wasusun; Panawan Suttiarporn.
Rice contains several kinds of bio-functional components. In this study, γ-aminobutyric acid (GABA) and Tocopherol, or Vitamin E were identified and their relationships to antioxidant activity using DPPH assay of both brown and pre germinated Thai rice varieties were studied. GABA are one of the two inhibitory neurotransmitters which make the neuron resistant to stimulation by excitatory neurotransmitters and inhibitor of some cancer cells activity whereas Tocopherol, or Vitamin E, which acts as antioxidant and improve blood cholesterol. Identification of GABA and Tocopherol in both brown and pre-germinated brown rice grown from different locations were conducted in eleven varieties, namely: Khao Dawk Mali 105, PTT1, CN1, RD31, Sang yod, RD6, Hom...
Tipo: PhysicalObject Palavras-chave: Antioxidant activity; Rice varieties; Tocopherol; DPPH assay; Brown rice; Pre-germinated brown rice; GABA; Gamma aminobutyric acid; Vitamin e; Nutritive value; Chemical composition; High performance liquid chromatography; HPLC; ฤทธิ์ต้านอนุมูลอิสระ; กาบา; โทโคฟีรอล; พันธุ์ข้าว; ข้าวกล้อง; ข้าวกล้องงอก; วิตามินอี; โครงสร้างทางเคมี; การวิเคราะห์องค์ประกอบทางเคมี; คุณค่าทางโภชนาการ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5713
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ความสัมพันธ์ของฤทธิ์ต้านอนุมูลอิสระกับปริมาณ tocopherol และ gamma aminobutyric acid ในข้าวกล้องและข้าวกล้องงอก Thai Agricultural
Pakinee Akkaravessapong; Angsutorn Wasusun; Suganya Wongpornchai.
Rice contains several kinds of bio-functional components. In this study, γ-aminobutyric acid (GABA) and tocopherol, or Vitamin E were identified and their relationships to antioxidant activity using DPPH assay of both brown and pre germinated Thai rice were studied. GABA, one of the two inhibitory neurotransmitters that make the neuron resistant to stimulation by excitatory neurotransmitters and inhibitor of some cancer cells activity. Tocopherols, or Vitamin E, are natural antioxidants which help improve blood cholesterol. Identification of GABA and tocopherol in both brown and pre-germinated brown rice grown from different locations were conducted in eleven varieties, namely: Khao Dawk Mali 105, PTT1, CNT1, RD31, Sang Yod, RD6, Hom Kradang-nga, Niaw Dam...
Tipo: PhysicalObject Palavras-chave: Anti oxidant activity; GABA; Tocopherol; DPPH assay; Brown rice; Pre-germinated brown rice; ข้าวกล้อง; ข้าวกล้องงอก; กาบา; โทโคฟีรอล; ฤทธิ์ต้านอนุมูลอิสระ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5596
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