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Comparative analysis of sulfated galactans from red algae by reductive hydrolysis and mild methanolysis coupled to two different HPLC techniques Inra
Quemener, B.; Lahaye, M..
The sugar determination of the sulfated galactans, agars and carrageenans of various red algae was performed using two different techniques of depolymerisation with subsequent HPLC analysis: 1) reductive hydrolysis/ HPAEC-PAD; 2) mild methanolysis/ RPLC-DR. Both techniques were optimized to release quantitatively the composite sugars (galactose, 6-O-methyl-galactose, the labile 3,6-anhydrogalactose and 2-O-methyl-3,6-anhydrogalactose residues) and precise relative response factors of authentic 3,6-anhydrogalactose were determined. The methanolysate neutralisation step, performed subsequently to methanolysis depolymerisation, was demonstrated as a key step for the quantitative recovery of the anhydrogalactose residues. The yield of the main sugars released...
Tipo: Journal Article Palavras-chave: GALACTANE SULFATE; ALGUE ROUGE; RHODOPHYCEAE; CARACTERISATION; CHROMATOGRAPHIE HPLC; CHROMATOGRAPHIE HPLC EN PHASE INVERSE; METHANOLYSE; CHROMATOGRAPHIE D'ECHANGE D'IONS; CHROMATOGRAPHIE GAZ LIQUIDE; GALACTANE SULFATED GALACTANS; RED ALGAE; REDUCTIVE HYDROLYSIS; HIGH PERFORMANCE ANION EXCHANGE CHROMATOGRAPHY; METHANOLYSIS; REVERSE PHASE LIQUID CHROMATOGRAPHY.
Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9800012150070225&uri=/notices/prodinra1/2010/11/
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