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Registros recuperados: 8
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A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance Inra
Moroldo, M.; Paillard, S.; Marconi, R.; Legeai, F.; Canaguier, A.; Cruaud, C.; de Berardinis, V.; Guichard, C.; Brunaud, V.; Le Clainche, I.; Scalabrin, S.; Testolin, R.; di Gaspero, G.; Morgante, M.; Adam-Blondon, A.F..
Background: Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed. Results: The physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic...
Tipo: Journal Article Palavras-chave: MILDIOU; MALADIE FONGIQUE; CARTE GENETIQUE; SYSTEME IMMUNITAIRE; VIGNE; RESISTANCE AUX MALADIES; CARTOGRAPHIE; GENE CANDIDAT; GENETIQUE; SEQUENCE NUCLEOTIDIQUE; CABERNET SAUVIGNON PLANT IMMUNE SYSTEM; VITIS VINIFERA L.; NONHOST RESISTANCE; POWDERY MILDEW; GENOME SEQUENCE; LINKAGE MAP; RICE GENOME; BAC; ARABIDOPSIS; GENETICS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011dc1cb8fa&uri=/notices/prodinra1/2011/03/
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CandidaDB : a genome database for Candida albicans pathogenomics Inra
D'Enfert, C.; Goyard, S.; Rodriguez-Arnaveilhe, S.; Frangeul, L.; Jones, L.; Tekaia, F.; Bader, O.; Albrecht, A.; Castillo, L.; Dominguez, A.; Ernst, J.F.; Fradin, C.; Gaillardin, C.; Garcia-Sanchez, S.; De Groot, P.; Hube, B.; Klis, F.M.; Krishnamurty, S.; Kunze, D.; Lopez, M.C.; Mavor, A.; Martin, N.; Moszer, I.; Onesime, D.; Perez Martin, J.; Sentandreu, R.; Valentin, E.; Brown, J.P..
CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology Center C.albicans genome sequence data by the European Galar Fungail Consortium. CandidaDB Release 2.0 (June 2004) contains information pertaining to Assembly 19 of the genome of C.albicans strain SC5314. The current release contains 6244 annotated entries corresponding to 130 tRNA genes and 5917 protein-coding genes. For these, it provides tentative functional assignments along with numerous pre-run analyses that can assist the researcher in the evaluation of gene function for the purpose of specific or large-scale analysis. CandidaDB is based on GenoList, a...
Tipo: Journal Article Palavras-chave: GENOME DATABASE; CANDIDA DB; ANNOTATION; GENOME SEQUENCE; GENE FUNCTION; EUROPEAN CONSORTIUM; FUNCTIONNAL ASSIGNEMENT.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2006c3705d94&uri=/notices/prodinra1/2007/12/
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Characterization of 41 microsatellite loci developed from the genome sequence of the poplar rust fungus, Melampsora larici-populina Inra
Xhaard, C.; Andrieux, A.; Halkett, F.; Frey, P..
Forty-one microsatellite markers were developed for the poplar rust fungus Melampsora larici-populina. The genome sequence of M. larici-populina strain 98AG31 was screened in silico for microsatellite loci using Magellan software. Thirty-nine loci, evaluated on a sexual population of 35 M. larici-populina isolates sampled on larch trees in the French Alps, were identified to be highly polymorphic. These markers revealed two to 34 alleles, with an average of 7.23 alleles per locus. The estimated heterozygosity ranged from 0.029 to 0.958 across the 41 microsatellite loci. These markers will be highly useful for population genetics studies of M. larici-populina.
Tipo: Journal Article Palavras-chave: BASIDIOMYCETE; MELAMPSORA LARICI-POPULINA; POPLAR RUST FUNGUS; GENOME SEQUENCE; MICROSATELLITES; M13-TAILED PRIMER METHOD.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e8fd96fd&uri=/notices/prodinra1/2010/10/
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Comparative mapping in Salicaceae: a tool for identifying important genes controlling adaptive traits Inra
Jorge, V.; Fabbrini, F.; Faivre-Rampant, P.; Fladung, M.; Gaudet, M.; Lagercrantz, U.; Leplé, J.C.; Morgante, M.; Pakull, B.; Sabatti, M.; Storme, V.; Taylor, G.; Vitacolonna, N.; DeWoody, J.; Bastien, C..
Comparative QTL mapping for adaptive traits among populations and species can lead to the identification of genomic regions conserved over a long evolutionary period and therefore potentially harboring genes of great importance for adaptive processes. At the same time, non-conserved QTLs could be responsible for local adaptation or interspecific differentiation.The availability of Populus as a model system for tree and woody perennial plant biology has largely been driven by the rapid development of genomic and molecular biology resources for this genus, culminating in the completion of a draft sequence of the Populus trichocarpa (black cottonwood) genome. Numerous genetic maps are available in different Populus and Salix species and QTLs for adaptive...
Tipo: Conference Paper Palavras-chave: GENE CANDIDAT; CARTE GENETIQUE; SEQUENCE GENOMIQUE; IDENTIFICATION DE GENES CANDIDATE GENE; GENETIC MAPPING; GENOME SEQUENCE; GENE IDENTIFICATION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105597576d&uri=/notices/prodinra1/2010/11/
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Naturally occurring nonpathogenic isolates of the plant pathogen species Pseudomonas syringae lack a type III secretion system and effector gene orthologues Inra
Mohr, T.J.; Liu, H.; Yan, S.; Morris, C.E.; Castillo, J.A.; Jelenska, J.; Vinatzer, B.A..
Pseudomonas syringae causes plant diseases, and the main virulence mechanism is a type III secretion system (T3SS) that translocates dozens of effector proteins into plant cells. Here we report the existence of a subgroup of P. syringae isolates that do not cause disease on any plant species tested. This group is monophyletic and most likely evolved from a pathogenic P. syringae ancestor through loss of the T3SS. In the nonpathogenic isolate P. syringae 508 the genomic region that in pathogenic P. syringae strains contains the hrp-hrc cluster coding for the T3SS and flanking effector genes is absent. P. syringae 508 was also surveyed for the presence of effector orthologues from the closely related pathogenic strain P. syringae pv. syringae B728a, but none...
Tipo: Journal Article Palavras-chave: PROGRAMMED CELL-DEATH; EXCHANGEABLE EFFECTOR; ESCHERICHIA-COLI; HYPERSENSITIVE RESPONSE; MICROBE INTERACTIONS; PROTEIN SECRETION; GENOME SEQUENCE; INNATE IMMUNITY; EFFECTOR PROTEINS; VIRULENCE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200850ea0c3b&uri=/notices/prodinra1/2008/06/
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The bdbDC operon of bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development Inra
Meima, R.; Eschevins, C.; Fillinger, S.; Bolhuis, A.; Hamoen, L.W.; Dorenbos, R.; Quax, W.J.; van Dijl, J.M.; Provvedi, R.; Chen, I.; Dubnau, D.; Bron, S..
The development of genetic competence in the Gram-positive eubacterium Bacillus subtilis is a complex postexponential process. Here we describe a new bicistronic operon, bdbDC, required for competence development, which was identified by the B. subtilis Systematic Gene Function Analysis program. Inactivation of either the bdbC or bdbD genes of this operon results in the loss of transformability without affecting recombination or the synthesis of ComK, the competence transcription factor. BdbC and BdbD are orthologs of enzymes known to be involved in extracytoplasmic disulfide bond formation. Consistent with this, BdbC and BdbD are needed for the secretion of theEscherichia coli disulfide bond-containing alkaline phosphatase, PhoA, by B. subtilis....
Tipo: Journal Article Palavras-chave: BACTÉRIE GRAM NÉGATIF; EXPRESSION DES GÈNES; OPERON; OXYDOREDUCTASE THIOLDISULFIDE; DÉVELOPPEMENT; FONCTION; GÉNÉTIQUE BACTÉRIENNE; SÉQUENCE NUCLÉOTIDIQUE; ACTIVITÉ ENZYMATIQUE ESCHERICHIA-COLI; BOND FORMATION; TRANSCRIPTION FACTOR; MOLECULAR-CLONING; GENOME SEQUENCE; DNA-BINDING; PROTEIN; GENE; IDENTIFICATION; TRANSFORMATION.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023579103391&uri=/notices/prodinra1/2010/10/
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The PhoP-PhoQ two-component regulatory system of Photorhabdus luminescens is essential for virulence in insects Inra
Derzelle, S.; Turlin, E.; Duchaud, E.; Pages, S.; Kunst, F.; Givaudan, A.; Danchin, A..
Photorhabdus luminescens is a symbiont of entomopathogenic nematodes. Analysis of the genome sequence of this organism revealed a homologue of PhoP-PhoQ, a two-component system associated with virulence in intracellular bacterial pathogens. This organism was shown to respond to the availability of environmental magnesium. A mutant with a knockout mutation in the regulatory component of this system (phoP) had no obvious growth defect. It was, however, more motile and more sensitive to antimicrobial peptides than its wild-type parent. Remarkably, the mutation eliminated virulence in an insect model. No insect mortality was observed after injection of a large number of the phoP bacteria, while very small amounts of parental cells killed insect larvae in less...
Tipo: Journal Article Palavras-chave: ENTOMOPATHOGENIC NEMATODE; GENOME SEQUENCE; BACTERIAL PATHOGENE; VIRULENCE; INSECT MODEL; MAGNESIUM.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008acbe701f&uri=/notices/prodinra1/2009/03/
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Whole-genome comparison between Photorhabdus strains to identify genomic regions involved in the specificity of nematode interaction Inra
Gaudriault, S.; Duchaud, E.; Lanois, A.; Canoy, A.S.; Bourot, S.; DeRose, R.; Kunst, F.; Boemare, N.; Givaudan, A..
The bacterium Photorhabdus establishes a highly specific association with Heterorhabditis, its nematode host. Photorhabdus strains associated with Heterorhabditis bacteriophora or Heterorhabditis megidis were compared using a Photorhabdus DNA microarray. We describe 31 regions belonging to the Photorhabdus flexible gene pool. Distribution analysis of regions among the Photorhabdus genus identified loci possibly involved in nematode specificity
Tipo: Journal Article Palavras-chave: PHOTORHABDUS; HETERORHABDITIS; GENE POOL; GENOME SEQUENCE; HYBRIDIZATION; CHROMOSOMAL SEQUENCE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200879731a17&uri=/notices/prodinra1/2009/01/
Registros recuperados: 8
Primeira ... 1 ... Última
 

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