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Registros recuperados: 14
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Analysis of protein changes during grape berry ripening by 2-DE and MALDI-TOF Inra
Giribaldi, M.; Perugini, I.; Sauvage, F.X.; Schubert, A..
Grape berry, a nonclimacteric fruit, during ripening turns from green, hard and acidic to coloured, soft and sweet. Many studies have focused on dynamic changes of mRNA levels, metabolites, sugars or individual proteins, but this is the first report of a proteomic approach applied to the screening of the most prominent variations that take place during berry ripening. Vitis vinifera cv. 'Nebbiolo Lampia' berries were collected at 10-day intervals, starting 1 month after flowering to complete ripe stage; total protein extracts from deseeded berries were separated by 2-DE. A total of 730 spots were detected in the 2-DE gels. 118 protein spots, differentially expressed during berry development, were subjected to MALDI-TOF analysis. Ninety-three of them were...
Tipo: Journal Article Palavras-chave: 2-DE; BERRY; GRAPEVINE; RIPENING.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008dd0c31e5&uri=/notices/prodinra1/2009/01/
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Characterization of ten novel Ty1/copia-like retrotransposon families of the grapevine genome Inra
Moisy, C.; Garrison, K.; Meredith, C. P.; Pelsy, F..
BACKGROUND: Retrotransposons make a significant contribution to the size, organization and genetic diversity of their host genomes. To characterize retrotransposon families in the grapevine genome (the fourth crop plant genome sequenced) we have combined two approaches: a PCR-based method for the isolation of RnaseH-LTR sequences with a computer-based sequence similarity search in the whole-genome sequence of PN40024.RESULTS: Supported by a phylogenic analysis, ten novel Ty1/copia families were distinguished in this study. To select a canonical reference element sequence from amongst the various insertions in the genome belonging to each retroelement family, the following screening criteria were adopted to identify the element sequence with: (1)...
Tipo: Journal Article Palavras-chave: RETROTRANSPOSON TY1-COPIA Ty1; RETROTRANSPOSON; COPIA-LIKE; GRAPEVINE; GENOME; PCR-BASED; RNASEH-LTR; GENETIC.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20089e1922fe&uri=/notices/prodinra1/2009/11/
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Characterization of viticultural terroirs using a simple field model based on oil depth - II. Validation of the grape yield and berry quality in the Anjou vineyard (France) Inra
Morlat, R.; Bodin, F..
Soil and climate are major constituents of the French notion of Terroir. This concept implies that there is a strong relationship between the composition of the grape, the characteristics of the wine and the territory of production. To study this link, a new method of characterization of the Terroir, including geological and pedological factors, was investigated. It uses a field model based on depth and clay content of soil, together with the degree of weathering of the parent rock. Consequently, for every type of parent rock belonging to a given geologic stage, there are a series of soils that show different stages of pedological evolution. According to the model, three kinds of soils are distinguished with regards to the weathering intensity of the...
Tipo: Journal Article Palavras-chave: TERROIR VITICOLE; VIGNE; SOL; CLIMAT; QUALITE DES BAIES; FACTEUR PEDOCLIMATIQUE; REGIME HYDRIQUE; DEBOURREMENT; MODELE FIELD SOIL MODEL; GRAPEVINE; BERRY COMPOSITION; WATER SUPPLY REGIME; TIMING OF BUDBURST.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20068b9f1d12&uri=/notices/prodinra1/2010/09/
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Characterization of Vitis vinifera L. somatic variants exhibiting abnormal flower development patterns Inra
Chatelet, P.; Laucou, V.; Fernandez, L.; Sreekanta, L.; Lacombe, T.; Martinez-Zapater, J.M.; Thomas, M.R.; Torregrosa, L..
Mutants have proven to be a key resource for functional genomic studies in model annual plant species. In perennial plant species where mutants are difficult to generate and to screen, spontaneous somatic variants represent a unique resource to understand the genetic control of complex developmental patterns. The morphological and histological characterization of six Vitis vinifera L. somatic variants that display four different abnormal phenotypes of flower development are described here. A phenotype of reiterated reproductive meristems (RRM), with both flower and petal reiteration, was observed in a somatic variant of the cultivar Carignan. An abnormal development of reproductive organs was displayed by the unfused carpels (UFC) somatic variant of cv....
Tipo: Journal Article Palavras-chave: ORGANE REPRODUCTEUR FLOWER DEVELOPMENT; GRAPEVINE; MADS-BOX; REPRODUCTIVE ORGANS; SOMATIC VARIANTS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20086f008e38&uri=/notices/prodinra1/2008/10/
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Contribution of runoff to incomplete off season water refilling in a Mediterranean vineyard Inra
Gaudin, R.; Celette, F.; Gary, C..
The dynamics of soil water was investigated in a Mediterranean vineyard during the 2003–2007 period in order to identify the inter-seasonal modification of water storage due to intercropping. The intercrop was a mixture of tall fescue and rye grass. Soil water content was measured on 3 m soil profiles (plus one 5 m profile) as was runoff from local stands. Great variations in soil refilling were observed over the years, for both the bare soil and intercrop treatments. The complete refilling occurred once and it was associated with a rise of the water table up to less than 2 m from the soil surface in the low part of the field. During the other years, the wetting front was identified between 1 m and 2.3 m at spring in the bare soil treatment, deeper in the...
Tipo: Journal Article Palavras-chave: CULTURE INTERCALAIRE; ENHERBEMENT RUNOFF; SOIL WATER REFILLING; GRAPEVINE; INTERCROPPING; WATER BALANCE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109d3da5c6&uri=/notices/prodinra1/2011/02/
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Development of resources for comparative physical mapping between Muscadinia rotundifolia and Vitis vinifera Inra
Zah-Bi, I.; Blanc, S.; Bras, M.; Canaguier, A.; Le Clainche, I.; Couturat, L.; Choisne, N.; Dry, I.; Gouyvenoux, M.; Merdinoglu, D.; Poulain, J.; Quesneville, H.; Wincker, P.; Mestre, P.; Adam-Blondon, A.F..
Tipo: Conference Paper Palavras-chave: MUSCADINIA ROTUNDIFOLIA; GRAPEVINE; DNA SEQUENCE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c779322e&uri=/notices/prodinra1/2011/01/
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Floral volatiles of Tanacetum vulgare L. attractive to Lobesia botrana Den. et Schiff. females Inra
Gabel, B.; Thiery, D.; Suchy, V.; Marion-Poll, F.; Hradsky, P.; Farkas, P..
The European grapevine moth (EGVM), Lobesia botrana, is a major pest of grapes in Europe. Females are attracted to a nonhost plant: tansy (Tanacetum vulgare L.), which is a common weed in Slovakian vineyards. A steam distillate extract of tansy flowers was analyzed by means of a GC-EAG technique to screen constituents detected by the olfactory receptors of EGVM females. From more than 200 GC peaks, nine peaks corresponding to monoterpenoids released an EAG response in more than 70% of the females (N=15):p-cymene,d-limonene,α-thujene,α-thujone,β-thujone, thujyl alcohol, terpinene-4-ol, (Z)-verbenol, and piperitone. The steam distillate of tansy as well as a synthetic blend of identified compounds released consistent attraction in a field cage. The use of...
Tipo: Journal Article Palavras-chave: LOBESIA BOTRANA; TANACETUM VULGARE; TANAISIE; LEPIDOPTERA; TORTRICIDAE; ATTRACTION; DEPREDATEUR; CHROMATOGRAPHIE EN PHASE GAZEUSE; SPECTROMETRIE DE MASSE; ELECTROANTENNOGRAPHIE; MEDIATEUR CHIMIQUE; TERPENOIDE LOBESIA BOTRANA; VITIS VINIFERA; TANACETUM VULGARE; TANSY; GRAPEVINE; LEPIDOPTERA; TORTRICIDAE; COMPOSITAE; PLANT-INSECT RELATIONSHIPS; OLFACTION; ALLELOCHEMICALS; TERPENOIDS; COUPLING GC-EAG.
Ano: 1992 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB920002500600028663&uri=/notices/prodinra1/2010/11/
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Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis Inra
Gamm, M.; Heloir, M.C.; Kelloniemi, J.P.; Poinssot, B.; Wendehenne, D.; Adrian, M..
The recent publication of the grapevine genome sequence facilitates the use of qRT-PCR to study gene expression changes. For this approach, reference genes are commonly used to normalize data and their stability of expression should be systematically validated. Among grapevine defenses is the production of the antimicrobial stilbenic phytoalexins, notably the highly fungitoxic pterostilbene, which plays a crucial role in grapevine interaction with Plasmopara viticola and Botrytis cinerea. As a resveratrol O-methyltransferase (ROMT) gene involved in pterostilbene synthesis was recently identified, we investigated the accumulation of the corresponding transcripts to those of two other stilbene biosynthesis related genes phenylalanine ammonia lyase (PAL) and...
Tipo: Journal Article Palavras-chave: GRAPEVINE; REFERENCE GENE; RESVERATROL O-METHYLTRANSFERASE.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20112b87a805&uri=/notices/prodinra1/2011/06/
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Inflorescence photosynthate allocation of Chardonnay grapevine (Vitis vinifera L.) fruiting cuttings Inra
Wojnarowiez, G.; Maillard, P.; Brechet, C.; Gross, P.; Clément, C.; Vaillant-Gaveau, N.; Fontaine, F..
Tipo: Meeting Paper Palavras-chave: INFLORESCENCE; PHOTOSYNTHESIS; GRAPEVINE; CO2 ASSIMILATION.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20094e4d7b47&uri=/notices/prodinra1/2009/07/
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Influence of ethylene on sucrose accumulation in grape berry Inra
Chervin, C.; Terrier, N.; Ageorges, A.; Ribes, F.; Kuapunyakoon, T..
Grape ripening is thought to be ethylene independent. However, Cabernet Sauvignon berries that were treated with 1-methylcyclopropene, a specific inhibitor of ethylene receptors, accumulated less sucrose than did controls over the following three weeks. This decreased sucrose accumulation was associated with a decreased RNA accumulation of two sucrose transporters (SUC11 and SUC12), whose expression is triggered at veraison when grape berries start to accumulate sugars. This preliminary study was performed over two consecutive years and suggests that the role of ethylene in grape ripening needs to be reconsidered and that it could be related to sugar accumulation
Tipo: Journal Article Palavras-chave: GRAPEVINE; ETHYLENE SIGNALING; SUCROSE TRANSPORT; RIPENING.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009d3e5f215&uri=/notices/prodinra1/2010/09/
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Potential effects of climate change on distribution and activity of insect vectors of grapevine pathogens Inra
Boudon, E.; Maixner, M..
The impact of changing climatic conditions on viticulture is currently mainly discussed with respect to alterations in grape physiology, adaptation of cultivars and cultural practice. However, pests and diseases of grapevine are influenced by changing climate, too. They are affected either directly through impacts on their life history and epidemiology or indirectly by changes of grapevine physiology and phenology.This presentation will focus on potential risks of changing climate caused by arthropod vectors of grapevine pathogens particularly in cool climate viticulture of the northern viticultural areas of Europe. More or less complex pathosystems consisting of grapevine, pathogens, vector (s) and sometimes alternative host plants need to be considered,...
Tipo: Conference Paper Palavras-chave: GRAPEVINE; VIRUS; PHYTOPLASMA; VECTOR; CLIMATE CHANGE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008d0be8eb2&uri=/notices/prodinra1/2008/06/
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Proteome changes in leaves from grapevine (Vitis vinifera L.) transformed for alcohol dehydrogenase activity Inra
Sauvage, F.X.; Pradal, M.; Chatelet, P.; Tesniere, C..
A proteomic approach has been used to study changes in leaf protein content from plants transformed for alcohol dehydrogenase (ADH) activity. Individual quantitative analysis of 190-436 spots separated by two-dimensional electrophoresis was performed, and spots displaying significant quantitative changes between control (C), sense (S), and antisense (R) transformants were selected using Student's t test. Of the 14 spots selected and further analyzed after trypsic digestion, 9 could be identified by MS analysis and 5 by LC-MS/MS. Identified proteins had mainly a chloroplastic origin: four rubisco large subunits, one rubisco binding protein, two glutamine synthetases, one elongation factor Tu, one ATP synthase beta subunit, and one plastidic aldolase....
Tipo: Journal Article Palavras-chave: ALCOHOL DEHYDROGENASE; GRAPEVINE; LEAVE; PROTEIN; TRANSFORMANT.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008425bb395&uri=/notices/prodinra1/2009/02/
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QTL of flavanoid synthesis and resistance to Plasmopara viticola Inra
Poutaraud Naidenov, A.; Hamm, G.; Prado, E.; Massot, C.; Merdinoglu-Wiedemann, S.; Coste, P.; Carre, V.; Muller, J.F.; Merdinoglu, D..
Tipo: Meeting Paper Palavras-chave: GRAPEVINE; FLAVONOIDS; RESISTANCE; PLASMOPARA VITICOLA; QTL.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109c49f933&uri=/notices/prodinra1/2011/01/
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Stilbene content in leaf discs of different grapevine genotypes treated with downy mildew (Plasmopara viticola) or UV-C Inra
Poutaraud, A.; Wiedemann-Merdinoglu, S.; Merdinoglu, D..
Tipo: Meeting Paper Palavras-chave: RAYONNEMENT UV STILBENE; GRAPEVINE; GENOTYPE; MILDEW; PLASMOPARA VITICOLA; UV-C; MUSCADINIA ROTUNDIFOLIA; CV SYRAH.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009f56e61b&uri=/notices/prodinra1/2010/11/
Registros recuperados: 14
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