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A method to determine volatile contaminants in polyethylene terephthalate (PET) packages by HDC-GC-FID and its application to post-consumer materials Ciênc. Tecnol. Aliment.
Fabris,Samanta; Freire,Maria Teresa de Alvarenga; Wagner,Roger; Reyes,Felix Guillermo.
A simple and low cost method to determine volatile contaminants in post-consumer recycled PET flakes was developed and validated by Headspace Dynamic Concentration and Gas Chromatography-Flame Ionization Detection (HDC-GC-FID). The analytical parameters evaluated by using surrogates include: correlation coefficient, detection limit, quantification limit, accuracy, intra-assay precision, and inter-assay precision. In order to compare the efficiency of the proposed method to recognized automated techniques, post-consumer PET packaging samples collected in Brazil were used. GC-MS was used to confirm the identity of the substances identified in the PET packaging. Some of the identified contaminants were estimated in the post-consumer material at concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extraction; Validation; Gas chromatography; PET packaging.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000400033
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A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) Electron. J. Biotechnol.
Barkley,Noelle A; Wang,Ming Li; Pittman,Roy N.
The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the...
Tipo: Journal article Palavras-chave: Fatty acid composition; Gas chromatography; Peanut (Arachis hypogaea L.); Real-time PCR; SNP genotyping.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009
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A system to evaluate the performance of hydraulic nozzles used in stored grain preotection trials Scientia Agricola
Vásquez-Castro,Javier A.; Baptista,Gilberto C. de; Gadanha Jr.,Casimiro D.; Trevizan,Luiz R.P..
We developed a system to evaluate the performance of hydraulic nozzles used in stored corn and wheat grain protection experiments. An insecticidal mix was used as test fluid to determine the transversal volumetric distribution and droplets spectrum of a model TJ-60 8002EVS hydraulic nozzle. A mobile application system was built to apply a rate equivalent to 5 L t-1 and obtain theoretical concentrations of 10 and 0.5 mg kg-1 of fenitrothion and esfenvalerate, respectively. The corn and wheat grains were spread out as a fine layer. Three glass slides (0.1 × 0.05 m) were placed on the top surface of the grains to ensure that the intended application rate was achieved. After treatment, the deposits on both matrices were analyzed by gas chromatography. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Application technology; Gas chromatography; Insecticide deposits; Corn; Wheat.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600001
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Accumulation and depuration of hydrocarbons in the mangrove oyster Crassostrea rhizophorae ArchiMer
Delcastillo, C; Corredor, J; Morell, J.
Adult specimens of Crassostrea rhizophorae were exposed to diesel oil dispersed in seawater. The experiment consisted of a 10 days contamination period and 15 days depuration period. Oyster samples were extracted, and analysed by gas chromatography and UV fluorescence spectroscopy. The concentrations of n-alkanes, branched alkanes, unresolved complex mixture (UCM) and aromatic hydrocarbons were determined. The oysters accumulated the hydrocarbons rapidly, reaching a maximum concentration of 387.5 ug/g (wet weight). After exposure to uncontaminated seawater, the oysters were able to reduce their hydrocarbon load to 5.6 ug/g. The data indicates that C. rhizophorae is capable of preferential accumulation and depuration of hydrocarbons. Apparently, the oysters...
Tipo: Text Palavras-chave: Crassostrea rhizophorae; Bivalvia; Fluorescence spectroscopy; Gas chromatography; Oyster culture; Hydrocarbons; Marine pollution; Accumulation; Self purification.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1602.pdf
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Análise de agrotóxicos organoclorados em camarão e pescado por cromatografia a gás com detector de micro captura de eletrons (GC-mECD). Repositório Alice
FERRACINI, V. L.; QUEIROZ, S. C. do N. de; ROSA, M. A.; SOUZA, D. R. C. de; QUEIROZ, J. F. de; PARAIBA, L. C..
Resumo: Resumo: Otimizou-se um método analítico para a determinação de multirresíduos de organoclorados em camarão marinho para a espécie Litopenaeus vannamei e em músculo de peixes da espécie Oreochromis sp., chamada popularmente de tilápia-vermelha. Utilizou-se a técnica de extração denominada QuEChERS, empregando cromatografi a a gás com detector de micro captura de elétrons (CG-mECD) para análise dos seguintes organoclorados em amostras de camarão e em músculo de peixes: hexaclorobenzeno, lindano, DDE, DDT, clorpirifós, endossulfan sulfato, endossulfan beta, endossulfan alfa, heptacloro, aldrin, endrin e dieldrin. O limite de detecção para todos os compostos foi de 0,0005 mg.g-1 e o limite de quantificação do método foi estabelecido em 0,005 mg.g-1. Os...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Multirresíduos; Camarão; Agrotóxico; Análise química; Cromatografia gasosa; Camarão; Peixe; Tilápia vermelha; Tilápia; Multiresidue analysis; Gas chromatography; Organochlorine pesticides; Shrimp; Litopenaeus vannamei; Oreochromis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1005834
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Análisis de residuos de organoclorados en los sedimentos de zonas de manglar en la Ciénaga Grande de Santa Marta y la Bahía de Chengue, Caribe colombiano OceanDocs
Espinosa, Luisa F.; Ramírez, Gustavo; Campos, Néstor H..
En los sedimentos superficiales aledaños al manglar, de cuatro estaciones dentro de la Ciénaga Grande de Santa Marta y una estación en la Bahía de Chengue, se analizaron las concentraciones de seis residuos de plaguicidas organoclorados (lindano, heptacloro, aldrin, pp'DDE, pp'DDD y pp'DDT), mediante la técnica de cromatografía gas-líquido. Se realizaron muestreos trimestrales entre marzo y diciembre de 1993, tratando de abarcar las principales épocas climáticas de la región (seca, lluviosa menor, seca menor y lluviosa mayor). Mediante análisis de varianza multifactorial se encontraron diferencias significativas solamente en las concentraciones de lindano, heptacloro y pp'DDE, entre las diferentes épocas climática; los otros tres compuestos (aldrin, pp'DDD...
Tipo: Journal Contribution Palavras-chave: Ecology; Chemical analysis; Salinity effects; Gas chromatography; Organic matter; Coastal lagoons; Estuaries; Mangroves; Ecology; Organic matter; Estuaries; Gas chromatography; Coastal lagoons; Mangroves; Http://aims.fao.org/aos/agrovoc/c_2467; Http://aims.fao.org/aos/agrovoc/c_5387; Http://aims.fao.org/aos/agrovoc/c_2673; Http://aims.fao.org/aos/agrovoc/c_11097; Http://aims.fao.org/aos/agrovoc/c_1697; Http://aims.fao.org/aos/agrovoc/c_16190.
Ano: 1999 URL: http://hdl.handle.net/1834/3804
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Analytical methods for mercury speciation in several matrixes: a review IPB - Escola Superior Agrária
Ramalhosa, Elsa; Río-Segade, Susana.
Mercury is one of the hazardous contaminants that may be present in the environment. Moreover, its toxicological effects are strongly dependent on the chemical form of the element, being the organic species the most toxic. In spite of this, the determination of mercury compounds in environmental samples, such as water, soils, sediments and biological matrixes, is of great importance. This review examines the current state of the sample treatments that have been developed, during the last 18 years (1990-2007),previous to separation and detection of mercury compounds, namely acid lixiviation, distillation,alkaline digestion and supercritical fluid extraction, as well as of the chromatographic techniques (gas chromatography and high performance liquid...
Tipo: Article Palavras-chave: Mercury; Speciation; Analytical methods; Gas chromatography; Liquid chromatography.
Ano: 2008 URL: http://hdl.handle.net/10198/3605
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Applications of electronic noses in meat analysis Ciênc. Tecnol. Aliment.
GÓRSKA-HORCZYCZAK,Elżbieta; GUZEK,Dominika; MOLĘDA,Zuzanna; WOJTASIK-KALINOWSKA,Iwona; BRODOWSKA,Marta; WIERZBICKA,Agnieszka.
Abstract Electronic noses are devices able to characterize and differentiate the aroma profiles of various food, especially meat and meat products. During recent years e-noses have been widely used in food analysis and proved to provide a fast, simple, non-expensive and non-destructive method of food assessment and quality control. The aim of this study is to summarize the most important features of this analytic tool and to present basic fields and typical areas of e-nose use as well as most commonly used sensor types and patterns for e-nose design. Prospects for the future development of this technique are presented. Methods and research results presented in this manuscript may be a guideline for practical e-nose use.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electronic nose; Sensors; Gas chromatography; Odour.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000300389
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Automação de Planilhas Eletrônicas Para o Cálculo de Índices de Retenção na Análise de Óleos Essenciais. Infoteca-e
GAMA, P. E.; BARBOZA, E. G.; SANTOS, M. C. da S.; BIZZO, H. R..
Na publicação é descrita a elaboração de uma planilha para a automação do cálculo dos índices de retenção empregados na identificação dos constituintes químicos de óleos essenciais. A planilha é constituída de um grupo de funções matemáticas pré-existentes na plataforma Microsoft Excel, estruturadas e combinadas de modo a automatizar o processo de interpolação dos dados de tempos de retenção necessários ao cálculo. O emprego da planilha resultou em redução no tempo gasto pelos profissionais envolvidos na análise, bem como na redução do retrabalho decorrente de erros de digitação, melhorando a eficiência e a qualidade dos resultados analíticos obtidos pelo laboratório.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Tecnologia de alimentos; Cromatografia gasosa; Kovats; Otimização; Chromatography; Gas chromatography.
Ano: 2017 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1081899
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Avaliação das condições de extração de voláteis de pimenta tabasco por HS-SPME. Repositório Alice
MESQUITA, W. S.; LUZ, A. B.; DA SILVA, L. C.; PEREIRA, R. de C. A.; GARRUTI, D. dos S..
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Aroma; Gas chromatography; Capsicum frutescens; Standardization; Aroma; Cromatografia em fase gasosa; Capsicum frutescens; Padronização.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1058697
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Cell wall polysaccharides of common beans (Phaseolus vulgaris L.) Ciênc. Tecnol. Aliment.
Shiga,Tânia M.; Lajolo,Franco M.; Filisetti,Tullia M.C.C..
The soluble and insoluble cotyledon (SPF-Co and IPF-Co) and tegument (SPF-Te and IPF-Te) cell wall polymer fractions of common beans (Phaseolus vulgaris) were isolated using a chemical-enzymatic method. The sugar composition showed that SPF-Co was constituted of 38.6% arabinose, 23.4% uronic acids, 12.7% galactose, 11.2% xylose, 6.4% mannose and 6.1% glucose, probably derived from slightly branched and weakly bound polymers. The IPF-Co was fractionated with chelating agent (CDTA) and with increasing concentrations of NaOH. The bulk of the cell wall polymers (29.4%) were extracted with 4.0M NaOH and this fraction contained mainly arabinose (55.0%), uronic acid (18.9%), glucose (10.7%), xylose (10.3%) and galactose (3.4%). About 8.7% and 10.6% of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legume; Pectin; Carbohydrate; Gas chromatography.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000200007
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Characterization of residual oils for biodiesel production Electron. J. Biotechnol.
Canesin,Edmilson Antonio; de Oliveira,Cláudio Celestino; Matsushita,Makoto; Felicidade Dias,Lucia; Reghiany Pedrão,Mayka; Evelazio de Souza,Nilson.
Background: Residual oils were characterized according to their physicochemical properties, i.e. acidity, iodine value, peroxide value and saponification number, to evaluate the degradation level and viability for biodiesel production. Results: The methyl esters of fatty acids (FAME) from samples of residual bovine, chicken and soybean oils were quantified by using four transesterification methods, using acidic and basic catalysis and, gas chromatography with flame ionization detector (GC-FID). Methods that used acidic catalysis at a lower temperature were the most efficient. Methyl biodiesel samples were synthesized by basic catalysis (KOH) for all quantified oils and the physicochemical properties of the biofuel were evaluated, i.e. viscosity, flash and...
Tipo: Journal article Palavras-chave: Biodiesel properties; Gas chromatography; Infrared; Transesterification methods.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100007
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Chemometric identification and nutritional evaluation of three species of Lutjanidae (Perciformes) from the Amazonian Atlantic Coast based on fatty acid profiles Acta Amazonica
ALMEIDA,Edilene Santos de; SILVA,Evaldo Martins da.
ABSTRACT The fatty acid (FA) profiles of fish species with a similar genetic or geographic origin are important quality parameters that attract bioecological interest because they are influenced by the nutritional habits of the species. The aim of this study was to determine the proximate compositions and FA profiles of heart, brain and muscle tissues from three fish species (Lutjanus purpureus, Lutjanus synagris and Ocyurus chrysurus) captured from the Amazonian Atlantic Coast. In addition to performing nutritional analysis of the FA profiles, principal components analysis (PCA) was performed as a chemometric tool to discriminate among the species. Lutjanus synagris had the highest protein content (18.23%), while O. chrysurus possessed the highest lipid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multivariate analysis; Gas chromatography; Lutjanus purpureus; Lutjanus synagris; Ocyurus chrysurus.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000400401
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Comparison of mate tea aromatic profiles by means of a descriptive panel and gas chromatography. Repositório Alice
BUTTERFIELD, T.; GODOY, R. C. B. de; KOPPEL, K..
bitstream/item/216370/1/Catie-Mate-Teas-SSP-Final.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mate; Chá; Cromatografia Gasosa; Yerba mate; Tea (beverage); Gas chromatography.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125237
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Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA BJMBR
Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A..
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019
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Convenient preparation of picolinyl derivatives from fatty acid esters ArchiMer
Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal.
It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation....
Tipo: Text Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf
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Desenvolvimento e validação de um método multiresíduo para determinação de pesticidas em água utilizando extração em fase sólida (SPE) e GC-MS/MS. Repositório Alice
SOUZA, D. R. C. de; FERRACINI, V. L.; QUEIROZ, S. C. do N. de; VILHENA, E.; NARDOCCI, A. C..
Um método para determinação multiresíduo de pesticidas baseado em cromatografia gasosa acoplada à espectrometria de massa (GC-MS/MS) foi desenvolvido, otimizado e validado para análises em amostras de águas. Foi utilizada extração em fase sólida (SPE- solid phase extraction) para concentrar as amostras em 1000 vezes e, alcançar a detectabilidade desejada. Para definir o procedimento de extração, foram avaliados parâmetros como pH e solvente de eluição. A melhor condição obtida foi validada depois de estabelecidos as seguintes condições otimizadas: pH= 7.0 e solvente de eluição diclorometano. O efeito matriz foi avaliado comparando-se a curva no solvente com a curva na matriz. O método proposto resultou no limite de detecção (LD) de 0,02µg/L e de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: SPE; Validação; Agrotóxico; Resíduo; Análise química; Cromatografia gasosa; Espectrometria; Água; Pesticide residues; Gas chromatography; Mass spectrometry; Water pollution.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084741
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Docosahexaenoic acid ethyl esther (DHAEE) microcapsule production by spray-drying: optimization by experimental design Ciênc. Tecnol. Aliment.
Rodrigues,Rodney Alexandre Ferreira; Rodrigues,Marili Villa Nova; Oliveira,Thais Isabel Vaccaro; Bueno,Cecilia Zorzi; Oliveira SOUZA,Ilza Maria de; Sartoratto,Adilson; Foglio,Mary Ann.
Docosahexaenoic acid is an essential polyunsaturated fatty acid with important metabolic activities. Its conjugated double bonds make it susceptible to decomposition. Its stability may be improved through fatty acid entrapment with a spray-drying technique; however, the many parameters involved in this technique must be considered to avoid affecting the final product quality. Therefore, this study aimed to evaluate the entrapment conditions and yields of fish oil enriched with docosahexaenoic acid ethyl ester. Microcapsules were obtained from Acacia gum using a spray-drying technique. The experimental samples were analyzed by chromatography and delineated by Statistica software, which found the following optimum entrapment conditions: an inlet temperature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulated fish oil; Gas chromatography; Response surface; Optimized conditions; Omega-3.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300007
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Evaluation of volatile profiles obtained for minimally-processed pineapple fruit samples during storage by headspace-solid phase microextraction gas chromatography-mass spectrometry Ciênc. Tecnol. Aliment.
TURAZZI,Francielle Crocetta; MORÉS,Lucas; MERIB,Josias; CARASEK,Eduardo; NARAIN,Narendra; LIMA,Lucélia Kátia de; NUNES,Maria Lúcia.
Abstract This paper describes the application of the solid-phase microextraction (SPME) technique for the determination and monitoring of the volatile profile of minimally-processed pineapple fruit stored at various temperatures (-12 °C, 4 °C and 25 °C) for different periods (1, 4 and 10 days). The SPME fiber coating composed of Car/PDMS presented the best performance. The optimal extraction conditions obtained through a Doehlert design were 60 min at 35 °C. The profiles for the volatile compounds content of the fruit at each stage of storage were determined by gas chromatography-mass spectrometry (GC-MS). The variation in the volatile profile over time was greater when the fruit samples were stored at 25 °C and at -12 °C compared to 4 °C. Thus, according...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Volatile profile; Pineapple; Minimally processed; Microextraction; Gas chromatography.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000400663
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Fenitrothion and esfenvalerate stability during corn and wheat sample processing Scientia Agricola
Vásquez-Castro,Javier Alberto; Baptista,Gilberto Casadei de; Trevizan,Luiz Roberto Pimentel; Gadanha Junior,Casimiro Dias.
The presence of insecticide residues in cereals represents a risk for the consumer, because these substances are used in large scale to protect stored grains from the attack of pests. The effects of three processing methods for corn and wheat samples on the stability of stored-grain protective insecticides were here evaluated. Fenitrothion and esfenvalerate were applied so as to produce theoretical concentrations of 10 and 0.5 mg kg-1, respectively. Two hours after treatment, the grains were processed and deposition was analyzed by gas chromatography. Grain species did not influence insecticide stability. This stability was only dependent upon the processing method and insecticide type. Grains processed together with dry ice provided the greatest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stored grain; Insecticide degradation; Dry ice; Gas chromatography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200008
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