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A large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, France, 2000 ArchiMer
Gallay, A; De Valk, H; Cournot, M; Ladeuil, B; Hemery, C; Castor, C; Bon, F; Megraud, F; Le Cann, Pierre; Desenclos, Jc.
A large waterborne outbreak of infection that occurred during August 2000 in a local community in France was investigated initially via a rapid survey of visits to local physicians. A retrospective cohort study was then conducted on a random cluster sample of residents. Of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). Those who had drunk tap water had a three-fold increased risk for illness (95% CI 2.4-4.0). The risk increased with the amount of water consumed (chi-square trend: p < 0.0001). Bacteriological analyses of stools were performed for 35 patients and virological analyses for 24 patients....
Tipo: Text Palavras-chave: Waterborne outbreak; Rotavirus; Norovirus; Gastroenteritis; Epidemiology; Campylobacter coli.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1696.pdf
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Analytical Methods for Virus Detection in Water and Food ArchiMer
Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane.
Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses...
Tipo: Text Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf
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Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 BJM
Portal,Thayara Morais; Siqueira,Jones Anderson Monteiro; Costa,Larissa Cristina Prado das Neves; Lima,Ian Carlos Gomes de; Lucena,Maria Silvia Sousa de; Bandeira,Renato da Silva; Linhares,Alexandre da Costa; Luz,Claudia Regina Nunes Eloi da; Gabbay,Yvone Benchimol; Resque,Hugo Reis.
ABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Norovirus; Sapovirus; Gastroenteritis; Children.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724
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Canine Parvovirus infection in puppies with gastroenteritis in Niterói, Rio de Janeiro, Brazil from 1995 to 1997 Braz. J. Vet. Res. Anim. Sci.
CUBEL GARCIA,Rita de Cássia Nasser; PINTO,Ana Maria Vianna; COSTA,Alexandre de Pina; MACIEL,Bianca Mendes; OLIVEIRA,Ledy do Horto dos Santos; NASCIMENTO,Jussara Pereira do; SANTOS,Adão Onofre dos; CASTRO,Maria Cristina Nobre e; WILLI,Liliane Maria Valentim; LABARTHE,Norma Vollmer.
Fecal samples from puppies with gastroenteritis less than 7 months old were examined for canine parvovirus infection (CPV-2) by hemagglutination (HA) and subsequent hemagglutination-inhibition (HI) tests. Forty of the 79 samples collected from April 1995 to June 1997 were found to be positive. About 70% of these samples were from 2 to 4 months old puppies, age in which they are at increased risk of developing CPV-2 infection, despite of vaccination. No seasonal distribution of canine parvovirus cases was found and it was supported by the results of a retrospective study realized at PolVet-UFF, which showed that gastroenteritis cases occurred throughout the year, for a six-year period (1991-97) in Niterói, Rio de Janeiro.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine parvovirus; Gastroenteritis; Haemagglutination tests.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200008
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Characterization of new recombinant noroviruses ArchiMer
Ambert Balay, K; Bon, F; Le Guyader, Soizick; Pothier, P; Kohli, E.
Noroviruses are important etiologic agents of acute gastroenteritis and show great genetic diversity. To characterize more fully previously detected strains that could not be assigned unequivocally to one particular genotype based on the RNA polymerase, we have sequenced a region in the capsid gene and, in some cases, in the junction between open reading frame 1 (ORF1) and ORF2. The results allowed us to identify several recombinant noroviruses: GGHb viruses were detected for the first time in France in August 2000 and then spread through France and to Europe during the following winter. Here we present the characterization of three other probable GII recombinants which showed different phylogenetic positions depending on their ORF1 and ORF2 sequences....
Tipo: Text Palavras-chave: Genogroup; RNA recombination; Gastroenteritis; Round structured virus; Reverse transcription; Norovirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1234.pdf
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Detection and genetic characterization of Mamastrovirus 5 from Brazilian dogs BJM
Alves,Christian D.B.T.; Budaszewski,Renata F.; Torikachvili,Marcela; Streck,André F.; Weber,Matheus N.; Cibulski,Samuel P.; Ravazzolo,Ana P.; Lunge,Vagner R.; Canal,Cláudio W..
Abstract Mamastrovirus 5 (MAstV5), belonging to the Astroviridae (AstV) family, previously known as canine astrovirus or astrovirus-like particles, has been reported in several countries to be associated with viral enteric disease in dogs since the 1980s. Astroviruses have been detected in fecal samples from a wide variety of mammals and birds that are associated with gastroenteritis and extra enteric manifestations. In the present study, RT-PCR was used to investigate the presence of MAstV5 in 269 dog fecal samples. MAstV5 was detected in 26% (71/269) of the samples. Interestingly, all MAstV5-positive samples derived from dogs displaying clinical signs suggestive of gastroenteritis, other enteric viruses were simultaneously detected (canine parvovirus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mamastrovirus 5; Canine astrovirus; Dog; Gastroenteritis; MAstV5.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300575
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Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil BJMBR
Xavier,M.P.T.P.; Oliveira,S.A.; Ferreira,M.S.R.; Victoria,M.; Miranda,V.; Silva,M.F.M.; Strina,A.; Barreto,M.L.; Miagostovicht,M.P.; Leite,J.P.G..
Acute gastroenteritis caused by viruses is one of the leading causes of infantile morbidity. The aim of the present study was to investigate the presence of human caliciviruses of the genera norovirus and sapovirus in children up to 3 years of age with acute gastroenteritis from low-income communities in the city of Salvador, Brazil. This study is an extension of previous work carried out to establish the profile of the most prevalent enteric pathogens present in these communities. In this report, 139 fecal samples, collected from July 2001 to January 2002 were analyzed by RT-PCR and 13 (9%) were positive for human caliciviruses. By sequencing, seven isolates were characterized as norovirus genogroup GII and one as sapovirus genotype GII/1. Sequencing of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calicivirus; Sapovirus; Astrovirus; Rotavirus A; Gastroenteritis; Genotypes.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500007
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Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil BABT
Pelayo,Jacinta Sanchez; Elias Junior,Antonio Roberto; Lima,Nicole Ribeiro de; Navarro,Armando; Rocha,Sérgio Paulo Dejato da.
Abstract Ground bovine meat is commonly consumed by the population of Brazil. However, it constitutes an excellent medium for the multiplication of microorganisms due to available nutrients and handling practices prior to consumption. Here, we examined 100 samples of ground beef for the presence of diarrheagenic Escherichia coli (DEC) pathotypes by PCR, and characterized isolates by analyzing their adherence to HEp-2 cells, serotype, antimicrobial susceptibility, and phylogeny. Enteroaggregative E. coli was detected in five (5%) meat samples, Shiga toxin-producing E. coli in three (3%), and atypical enteropathogenic E. coli in two (2%). According to the phylogeny, six isolates (60%) were classified in group A, two (20%) in group B1, and two (20%) in group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrheagenic Escherichia coli; Ground beef; Gastroenteritis; Serotyping.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100502
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Detection of double-stranded RNA viruses in fecal samples of dogs with gastroenteritis in Rio de Janeiro, Brazil Arq. Bras. Med. Vet. Zootec.
Costa,A.P.; Cubel Garcia,R.C.N.; Labarthe,N.V.; Leite,J.P.G..
Colheram-se 163 amostras fecais no período de 1995 a 2001 para investigar a ocorrência da infecção por parvovírus e rotavírus em cães com gastrenterite utilizando-se a técnica de eletroforese em gel de poliacrilamida. Em três amostras observou-se a presença do genoma bisegmentado similar ao perfil eletroforético dos picobirnavírus (PBV) e em uma, três segmentos de RNA dupla fita, característico de picotrirnavírus. Das amostras positivas para PBV, duas foram obtidas de filhotes e uma foi positiva para parvovírus canino. Este é o primeiro relato da detecção de vírus com genoma bisegmentado em cães com diarréia no Estado do Rio de Janeiro.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dog; Picobirnavirus; Gastroenteritis; Polyacrylamide gel electrophoresis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400020
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Epidemiological aspects of rotavirus infections in Minas Gerais, Brazil BJID
Silva,Maria Luzia da Rosa e; Naveca,Felipe Gomes; Carvalho,Iná Pires de.
In order to develop good polices regarding public health measures and vaccine use to prevent rotavirus induced gastroenteritis, the epidemiology of the illness in various regions of Brazil is necessary. Accordingly, this study was to detect the frequency and types of rotavirus in one city in a tropical part of Brazil. This is an epidemiological survey of pediatric gastroenteritis caused by rotavirus conducted in Juiz de Fora, Minas Gerais State, Brazil. We analyzed 656 in-patient (190) and out-patient (466) stool samples from children ages 0 to 5 years during 1998. Rotavirus detection was performed using polyacrylamide gel electrophoresis (PAGE). Rotavirus was isolated from 62/190 stool samples (32.6%) from hospitalized children and 16/466 (3.4%) from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Gastroenteritis; Epidemiological survey; Seasonality; Electrophoretic patterns.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000400007
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Genomic characterization of Canine circovirus detected in a dog with intermittent hemorrhagic gastroenteritis in Brazil Ciência Rural
Cruz,Taís Fukuta; Batista,Thiago Neves; Vieira,Ester Mariane; Portela,Luiz Marcos Frediani; Baccarin,Amanda Mahnke; Gradiz,Jéssica Jehle; Araújo Junior,João Pessoa.
ABSTRACT: Because Canine circovirus (CanineCV) is a new species of the genus Circovirus, several issues related to its epidemiology, pathogenesis and clinical disease remain unknown. Thus, this study aimed to perform the characterization of the first complete genome sequence of CanineCV detected in a dog with diarrhea in Brazil. A stool sample was collected of a ten-month-old female German Shepherd dog which had signs of intermittent hemorrhagic gastroenteritis, vomiting, and a history of eating raw pork. The complete CanineCV genome was sequenced by Next-Generation Sequencing. The sequence had 2,063 nucleotides, showed a typical genomic organization for circovirus, and was grouped with strain 214 described in the United States by phylogenetic analysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Circovirus; Canine circovirus; Genomic characterization; Gastroenteritis.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500451
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Molecular epidemiology of caliciviruses detected in sporadic and outbreak cases of gastroenteritis in France from December 1998 to February 2004 ArchiMer
Bon, Fabienne; Ambert Balay, K; Giraudon, H; Kaplon, J; Le Guyader, Soizick; Pommepuy, Monique; Gallay, A; Vaillant, V; De Valk, H; Chikhi Brachet, R; Flahaut, A; Pothier, P; Kohli, E.
We compiled sequence and epidemiological data from 172 caliciviruses detected in France from December 1998 to February 2004 in sporadic and outbreak cases. The results showed a cocirculation of strains with a majority of genogroup II (GII) noroviruses. Three groups of noroviruses, not detected before in our laboratory, emerged and spread during the period: the recombinant GGIIb and Norwalk-related strains not amplified in the polymerase gene in 2000 and a new Lordsdale variant in 2002. We observed that (I) GII4 noroviruses were predominant in nursing home and hospital outbreaks but rare in oyster- and water-related outbreaks despite continuous circulation in the population; (ii) at the opposite, genogroup I strains were detected in the majority of...
Tipo: Text Palavras-chave: Genogroup; Cocirculation; Gastroenteritis; Caliciviruses; Epidemiological.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-723.pdf
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Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil BJMBR
Gallimore,C.I.; Barreiros,M.A.B.; Brown,D.W.G.; Nascimento,J.P.; Leite,J.P.G..
Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contaminated oysters. This study describes the application of a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerase-coding region of the norovirus genome, and DNA sequencing for the detection and preliminary characterisation of noroviruses in outbreaks of gastroenteritis in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Noroviruses; Day care center; Gastroenteritis; Genotypes; Introduction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300005
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Possible relation between a winter epidemic of acute gastroenteritis in France and viral contamination of shellfish ArchiMer
Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique.
Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between...
Tipo: Text Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf
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Rotavirus gastroenteritis in a children's hospital specialized in craniofacial malformations J. Venom. Anim. Toxins incl. Trop. Dis.
Vieira,NA; Pereira,PCM; Carvalho,LR.
This study aimed to verify the relationship between acute diarrhea provoked by rotavirus and different indicators of craniofacial malformations. In the Hospital for Rehabilitation of Craniofacial Anomalies, 8,724 children with cleft lip and cleft palate were divided into the following groups: acute diarrhea and infection due to rotavirus (C1, n = 62), acute diarrhea (C2, n = 153) and without acute diarrhea (C3, n = 8,509). In C1, 29.03% of the cases consisted of hospital infections associated with the hospitalization period while 38.71% of the patients were aged less than six months. The percentage of children not having breastfed was significantly higher in acute diarrhea groups. Additionally, there was a seasonal prevalence of rotavirus infection between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Gastroenteritis; Diarrhea; Child.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200015
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Rotavirus infection in children and adult patients attending in a tertiary Hospital of São Paulo, Brazil BJID
Carraro,Emerson; Perosa,Ana Helena Sitta; Siqueira,Itacy; Pasternak,Jacyr; Martino,Marines Dalla Vale.
During the period of January 2003 to December 2005, 3,768 stool samples were received in the Microbiology Laboratory for rotavirus antigen detection from outpatients and inpatients of Albert Einstein Hospital, SP. Fresh stool samples from children and adults were analyzed by two methodologies: during 2003 and 2004 by latex agglutination (Slidex Rotavirus, Biomerieux) and 2005 by an immunochromatographic assay for the combined detection of rotavirus and adenovirus (Vikia Rota-Adeno, Biomerieux). Rotavirus group A was detected in 755 (20%) samples. The annual prevalence was 19.8% in 2003, 21.7% in 2004, and 18.7% in 2005. Rotavirus was detected every month during the period of the study, with peak of positivity between June and August (>35%). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Gastroenteritis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100010
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Scirrhous eosinophilic gastritis in two dogs Ciência Rural
Laisse,Cláudio João Mourão; Castro,Neusa Barbosa; Oliveira,Eduardo Conceição de; Bianchi,Matheus Viezzer; Fredo,Gabriela; Siviero,Amanda dos Santos; Lopes,Daniela Jardim; Pavarini,Saulo Petinatti; Driemeier,David; Sonne,Luciana.
ABSTRACT: Scirrhous eosinophilic gastritis (SEG) is characterized by diffuse infiltration of eosinophils and concomitant fibrovascular tissue proliferation in the stomach wall. The condition is a rare occurrence with an unknown cause. Two dogs were examined with a history of progressive weight loss, emesis, melena and anorexia. Complete blood cell counts of both cases revealed anemia and eosinophilia, while ultrasonography examination revealed thickening of the stomach wall. Due to suspected gastric neoplasia and a poor clinical prognosis, the dogs were euthanized. At necropsy, both had markedly distended stomachs with a diffusely thickened and firm wall. The spleens of both dogs were enlarged with a nodular surface. Histological evaluation revealed...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Dog; Eosinophilia; Gastroenteritis; Gastropathy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500881
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Serological and molecular diversity of human rotavirus in São Paulo, Brazil BJM
Munford,Veridiana; Souza,Eloisa Correia; Caruzo,Thabata Alessandra Ramos; Martinez,Marina Baquerizo; Rácz,Maria Lúcia.
From a total of 187 fecal samples from children with ages between 0 and 5 years, collected in the Hospital Universitário -USP, Brazil, from 1994 to 1996, 54 (28.9%) were positive for rotavirus. Positive samples were characterized by electropherotyping, subgrouping, G serotype and genotype and P genotype. Rotavirus electropherotypes were characterized in four different long genome patterns (38.9%), one short genome pattern (34.0%) and 18.0% were characterized as an unusual pattern. Subgroup I was found in 38.9% strains, subgroup II in 50.0% and 7.7% was subgroup nonI-nonII. For G serotypes, G2 was found in 59.3%, G1 was identified in 33.3% of strains, two samples showed mixtures of G1+G2 and one sample was G1+G3. Ten samples characterized as serotype G2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Gastroenteritis; PCR; Serotypes; Genotypes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300014
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THE ECONOMIC BENEFITS OF BREASTFEEDING: A REVIEW AND ANALYSIS AgEcon
Weimer, Jon P..
A minimum of $3.6 billion would be saved if breastfeeding were increased from current levels (64 percent in-hospital, 29 percent at 6 months) to those recommended by the U.S. Surgeon General (75 and 50 percent). This figure is likely an underestimation of the total savings because it represents cost savings from the treatment of only three childhood illnesses: otitis media, gastroenteritis, and necrotizing enterocolitis. This report reviews breastfeeding trends and previous studies that assessed the economic benefits of breastfeeding.
Tipo: Report Palavras-chave: Breastfeeding; Economic benefits; Otitis media; Gastroenteritis; Necrotizing enterocolitis; Food Consumption/Nutrition/Food Safety.
Ano: 2001 URL: http://purl.umn.edu/33813
Registros recuperados: 19
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