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Registros recuperados: 5
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An alternative method for Staphylococcus aureus DNA isolation. Repositório Alice
CHAPAVAL, L.; MOON, D. H.; GOMES, J. E.; DUARTE, F. R.; TSAI, S. M..
Metodologia alternativa para extração de DNA genômico de Staphylococcus aureus. Abstract: This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes ( A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction ( HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 105 cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25 mu l. Metodologia alternativa para extração de DNA genômico de Staphylococcus aureus. Resumo: Descreve-se um procedimento rápido para extração de DNA...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Staphylococcus aureus; DNA genômico; Extração de DNA; Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/534206
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Giovani. Variabilidade de parâmetros biológicos e genéticos de Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) no Brasil. Repositório Alice
SMANIOTTO, G..
2016
Tipo: Tese/dissertação (ALICE) Palavras-chave: Controle biológico; Temperatura; Diatraea saccharalis; PCR; DNA genômico; Biological control; Temperature; Diatraea saccharalis; PCR; Genomic DNA.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1056433
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Molecular cloning, expression and characterization of a serine proteinase from Japanese edible mushroom, Grifola frondosa: solving the structure - function anomaly of a reported aminopeptidase Electron. J. Biotechnol.
Islam,Mohammed M.
The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other and could not be separated by Ni-NTA...
Tipo: Journal article Palavras-chave: 5'-RACE; CDNA; Genomic DNA; Maitake; Refolding.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400010
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NeuroSNP: Tool to Filter SNPs in Whole Genomic DNA. Repositório Alice
ZONOVELLI, B.; BORGES, C. C. H.; ARBEX, W. A..
2014
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Bioinformatics; Genomic DNA; SNP Filtering; Machine Learning; Computational Intelligence; Neural Network.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1019933
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Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landracesof Solanum tuberosum and Zea mays Ciencia e Investigación Agraria
Barra,Macarena; Salazar,Erika; Beltrán,María; Sagredo,Boris.
The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies ofmolecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young...
Tipo: Journal article Palavras-chave: Activated charcoal; DNA extraction; Genomic DNA; Polyphenols.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300019
Registros recuperados: 5
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