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Registros recuperados: 14
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Anopheles gambiae PRS1 modulates plasmodium development at both midgut and salivary gland steps Inra
Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I..
Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not...
Tipo: Journal Article Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/
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Antennal pathways in the central nervous system of a blood-sucking bug, Rhodnius prolixus Inra
Barrozo, R.B.; Couton, L.; Lazzari, C.R.; Insauti, T.C.; Minoli, S.A.; Fresquet, N.; Rospars, J.P.; Anton, S..
The haematophagous bug Rhodnius prolixus has been a model system in insect physiology for a long time. Recently, several studies have been devoted to its sensory systems, including olfaction. However, few data are available on the basic organisation of the nervous system in this species. By means of neuronal backfills, histology, confocal microscopy and three-dimensional reconstruction methods, we have characterized the projection patterns of antennal sensory neurons within the central nervous system of this disease-vector insect. We established the first partial three-dimensional map of the antennal lobe (AL) of a hemipteran insect. The ALs of this species are relatively diffuse structures, which nevertheless show a glomerular organisation. Based on...
Tipo: Journal Article Palavras-chave: RHODNIUS PROLIXUS; ARTHROPODA; HEXAPODA; INSECTE; HEMIPTERA; SYSTEME NERVEUX CENTRAL; NEURONE; OLFACTION; HISTOLOGIE; MICROSCOPIE CONFOCALE; CARTE TRIDIMENSIONNELLE; LOBE ANTENNAIRE; GLOMERULE; HETEROPTERA; REDUVIIDAE ANTENNAL LOBE; THREE-DIMENSIONAL RECONSTRUCTION; QUANTITATIVE ANALYSIS; DISEASE-VECTOR INSECT; ANTENNAL PROJECTIONS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f9cf078d&uri=/notices/prodinra1/2010/11/
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Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant Inra
Durand, N.; Carot-Sans, G.; Chertemps, T.; Bozzolan, F.; Party, V.; Renou, M.; Debernard, S.; Rosell, G.; Maïbèche-Coisne, M..
Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/ pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In...
Tipo: Journal Article Palavras-chave: LEPIDOPTERA; NOCTUIDAE; HEXAPODA; PCR; ADNC; SDS PAGE; EXPRESSION DES GENES; ELECTROPHORESE; CARBOXYLESTERASE; SPODOPTERA LITTORALIS; OLFACTION; ODEEUR; PLANTE HOTE; INSECTE; ENZYME; BIOLOGIE MOLECULAIRE; BIOCHIMIE; ELECTROPHYSIOLOGIE; PHEROMONE; PHEROMONE SEXUELLE; RECOMBINAISON; PROTEINE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010229c7cd1&uri=/notices/prodinra1/2011/05/
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Characterization of ecdysteroids in Drosophila melanogaster by enzyme immunoassay and nano-liquid chromatography–tandem mass spectrometry Inra
Blais, C.; Blasco, T.; Maria, A.; Dauphin-Villemant, C.; Lafont, R..
Ecdysteroids are polyhyclroxylated steroids that function as molting hormones in insects. 20-Hydroxyecdysone (a 27C-ecdysteroid) is classically considered as the major steroid hormone of Drosophila melanogaster, but this insect also contains 28C-ecdysteroids. This arises from both the use of several dietary sterols as precursors for the synthesis of its steroid hormones, and its inability to dealkylate the 28C-phytosterols to produce cholesterol. The nature of Drosophila ecdysteroids has been re-investigated using both high-performance liquid chromatography coupled to enzyme immunoassay and a particularly sensitive nano-liquid chromatography-mass spectrometry methodology, while taking advantage of recently available ecdysteroid standards isolated from...
Tipo: Journal Article Palavras-chave: DROSOPHILE; ARTHROPODA; HEXAPODA; INSECTE; SPECTROMETRIE DE MASSE; ECDYSTEROIDE; HORMONE; HORMONE DE MUE; ENZYME; CHROMATOGRAPHIE LIQUIDE; HPLC; TECHNIQUE IMMUNOLOGIQUE DROSOPHILA; DEVELOPMENT; ECDYSONE; MASS SPECTROMETRY; PHYTOSTEROL; STEROL.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e5525b42&uri=/notices/prodinra1/2011/02/
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Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae Inra
Brigaud, I.; Grosmaître, X.; Francois, M.C.; Jacquin-Joly, E..
In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive...
Tipo: Journal Article Palavras-chave: CLONAGE; LOBE ANTENNAIRE; NOCTUIDAE; MITE; HEXAPODA; ARTHROPODA; AMINE BIOGENIQUE; RECEPTEUR; CLONAGE MOLECULAIRE; ADNC; POLYMORPHISME; PCR; PHEROMONE; LEPIDOPTERA; MAMESTRA BRASSICAE OCTOPAMINE/TYRAMINE RECEPTOR; EXPRESSION PATTERN; OLFACTION; OLFACTORY PLASTICITY.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201084fc8d7c&uri=/notices/prodinra1/2010/11/
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Cloning of an octopamine/tyramine receptor and plasticity of its expression as a function of adult sexual maturation in the male moth Agrotis ipsilon Inra
Duportets, L.; Barrozo, R.B.; Bozzolan, F.; Gaertner, C.; Anton, S.; Gadenne, C.; Debernard, S..
In the male moth Agrotis ipsilon behavioural response and antennal lobe (AL) neuron sensitivity to the female-produced sex pheromone increase with age and juvenile hormone (JH) level. We recently showed that the neuromodulator, octopamine (OA), interacts with JH in this age-dependent olfactory plasticity. To further elucidate its role, we cloned a full cDNA encoding a protein that presents biochemical features essential to OA/tyramine receptor (AipsOAR/TAR) function. The AipsOAR/TAR transcript was detected predominantly in the antennae, the brain and, more specifically, in ALs where its expression level varied concomitantly with age. This expression plasticity indicates that AipsOAR/TAR might be involved in central processing of the pheromone signal during...
Tipo: Journal Article Palavras-chave: LOBE ANTENNAIRE; LEPIDOPTERA; NOCTUIDAE; AGROPIS IPSILON; RECEPTEUR; OLFACTION; MITE; INSECTE; MATURATION SEXUELLE; ARTHROPODA; HEXAPODA; NEURONE; PHEROMONE; HORMONE; ADNC; CLONAGE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL ANTENNAL LOBE; OCTOPAMINE/TYRAMINE RECEPTOR; OLFACTORY PROCESSING; MATURATION; EXPRESSION PLASTICITY.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102d18e75d&uri=/notices/prodinra1/2011/02/
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Hedonic taste in Drosophila revealed by olfactory receptors expressed in taste neurons Inra
Hiroi, M.; Tanimura, T.; Marion-Poll, F..
Taste and olfaction are each tuned to a unique set of chemicals in the outside world, and their corresponding sensory spaces are mapped in different areas in the brain. This dichotomy matches categories of receptors detecting molecules either in the gaseous or in the liquid phase in terrestrial animals. However, in Drosophila olfactory and gustatory neurons express receptors which belong to the same family of 7-transmembrane domain proteins. Striking overlaps exist in their sequence structure and in their expression pattern, suggesting that there might be some functional commonalities between them. In this work, we tested the assumption that Drosophila olfactory receptor proteins are compatible with taste neurons by ectopically expressing an olfactory...
Tipo: Journal Article Palavras-chave: NEURONE; RECEPTEUR; PROTEINE; SENSATION; GOUT; FONCTION PHYSIOLOGIQUE; INSECTE; ARTHROPODA; HEXAPODA; STIMULUS; SUCRE; CAFEINE  OLFACTION; NEURON; PROTEIN RECEPTOR; TASTE; DROSOPHILA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201058c69fd&uri=/notices/prodinra1/2010/10/
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Molecular basis of female-specific odorant responses in Bombyx mori Inra
Anderson, A.R.; Wanner, K.W.; Trowell, S.C.; Warr, C.G.; Jacquin-Joly, E.; Zagatti, P.; Robertson, H.; Newcomb, R.D..
Males and females of many moth species exhibit important differences in sexual behaviours. Much research in this field has focused on the male-specific behaviour, electrophysiology and molecular biology of sex pheromone reception. Female-specific behaviours have been less well studied although, like male-specific behaviours, they could provide opportunities for intervention and management of lepidopteran pests. Previously, we identified genes encoding putative odorant receptors (ORs) from the genome of the silkworm, Bombyx mori, some of which have higher levels of steady-state transcript in the antennae of adult females compared with males. We have identified the full-length cDNA sequences of some of these ORs and described a novel OR that is part of a...
Tipo: Journal Article Palavras-chave: TERPENE; ACIDE BENZOIQUE; PHENYLETHANOL; BENZALDEHYDE; ELECTROPHYSIOLOGIE; SEQUENCE NUCLEOTIDIQUE; ODEUR; HYPOTHENEMUS HAMPEI; ARTHROPODA; HEXAPODA; INSECTE; RECEPTEUR; PLANTE; COMPOSE VOLATILE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL; ADNC; BIOLOGIE MOLECULAIRE; LEPIDOPTERA BOMBYX MORI; OLFACTION; ODORANT RECEPTORS; PLANT VOLATILES; PHEROMONE.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101ffb559b&uri=/notices/prodinra1/2010/11/
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Ordinary glomeruli in the antennal lobe of male and female tortricid moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae) process sex pheromone and host-plant volatiles Inra
Varela, N.; Avilla, J.; Gemeno, C.; Anton, S..
Both sexes of Grapholita molesta, a key pest of stone fruits, are able to detect host-plant volatiles and the sex pheromone emitted by females, and to modify their behaviour accordingly. How olfactory information is processed in the central nervous system is unknown. Intracellular recordings and stainings were used to characterize antennal lobe (AL) neuron responses to single pheromone components, a behaviourally active blend of five peach volatiles and a pear-fruit ester. AL neurons with different response patterns responded to pheromone components and plant volatiles. In males more neurons responded specifically to the main pheromone component than in females, whereas neurons responding to all three pheromone components were more abundant in females....
Tipo: Journal Article Palavras-chave: LOBE ANTENNAIRE; CYDIA MOLESTA; ARTHROPODA; HEXAPODA; TORTRICIDAE; INSECTE; PHEROMONE SEXUELLE; ATTRACTIF SEXUEL; NEURONE; ODEUR; GLOMERULE GRAPHOLITA MOLESTA; OLFACTION; ANTENNAL LOBE; PHEROMONE; INTRACELLULAR RECORDING AND STAINING.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201157fcd7c0&uri=/notices/prodinra1/2011/03/
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Plant terpenes affect intensity and temporal parameters of pheromone detection in a moth Inra
Party, V.; Hanot, C.; Said, I.; Rochat, D.; Renou, M..
In moths, the components of the female pheromone blend are detected in the male antennae by pheromone olfactory receptor neurons (Ph-ORNs) expressing narrowly tuned olfactory receptors. Responses to sex pheromones have generally been thought to be independent from the odorant background. However, interactions between pheromone components and plant volatiles have been reported at behavioral and detection levels. To document the mechanisms of such interactions, we analyzed Ph-ORN responses of Spodoptera littoralis to the main pheromone component, Z9E11-14:Ac, in the presence of 4 monoterpenes. To mimic natural contexts in which plant odors and pheromone emanate from different sources, the 2 stimuli were presented with different temporal patterns and from...
Tipo: Journal Article Palavras-chave: SPODOPTERA LITTORALIS; NOCTUIDAE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; PLANTE; COMPOSE VOLATILE; MITE; MONOTERPENE; PHEROMONE; ATTRACTIF SEXUEL; ODEUR  ODORANT INTERACTIONS; OLFACTION; OLFACTORY RECEPTOR NEURONS; PLANT VOLATILE COMPOUNDS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e254489a&uri=/notices/prodinra1/2010/10/
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Quantitative analysis of sex-pheromone coding in the antennal lobe of the moth Agrotis ipsilon: a tool to study network plasticity Inra
Jarriault, D.; Gadenne, C.; Rospars, J.P.; Anton, S..
To find a mating partner, moths rely on pheromone communication. Released in very low amounts, female sex pheromones are used by males to identify and localize females. Depending on the physiological state (i.e. age, reproductive state), the olfactory system of the males of the noctuid moth Agrotis ipsilon is `switched on or off'. To understand the neural basis of this behavioural plasticity, we performed a detailed characterization of the qualitative, quantitative and temporal aspects of pheromone coding in the primary centre of integration of pheromonal information, the macroglomerular complex (MGC) of the antennal lobe. MGC neurons were intracellularly recorded and stained in sexually mature virgin males. When stimulating antennae of males with the...
Tipo: Journal Article Palavras-chave: AGROTIS IPSILON; ARTHROPODA; HEXAPODA; INSECTE; PHEROMONE; MITE; PLASTICITE; ATTRACTIF SEXUEL; NEURONE; OLFACTION; LOBE ANTENNAIRE OLFACTORY CODING; MOTH; ANTENNAL LOBE; SEX-PHEROMONE; PROJECTION NEURON; SPIKE TRAIN ANALYSIS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010352d8cb4&uri=/notices/prodinra1/2010/10/
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The 20-hydroxyecdysone-induced signalling pathway in G2/M arrest of Plodia interpunctella imaginal wing cells Inra
Siaussat, D.; Bozzolan, F.; Porcheron, P.; Debernard, S..
The mechanisms involved in the control of cellular proliferation by the steroid hormone 20-hydroxyecdysone (20E) in insects are not known. We dissected the 20E signalling pathway responsible for G2/M arrest of imaginal cells from the IAL-PID2 cells of the Indian meal moth Plodia interpunctella. We first used a 5′–3′ RACE-based strategy to clone a 4479 bp cDNA encoding a putative P. interpunctella HR3 transcription factor named PiHR3. The deduced amino acid sequence of PiHR3 was highly similar to those of HR3 proteins from other lepidopterans, e.g. Manduca sexta and Bombyx mori. Using double-stranded RNA-mediated interference (dsRNAi), we then succeeded in blocking the ability of 20E to induce the expression of PiEcR-B1, PiUSP-2 and PiHR3 genes that encode...
Tipo: Journal Article Palavras-chave: PLODIA INTERPUNCTELLA; EUCARYOTE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; HORMONE STEROIDE; MITE; CLONAGE; ADNC; EXPRESSION DES GENES; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; ACIDE AMINE  RNA INTERFERENCE; STEROID HORMONE RECEPTOR SUPERFAMILY; G2/M ARREST; B CYCLIN; SIGNALLING PATHWAY; IMAGINAL CELLS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103c688c9f&uri=/notices/prodinra1/2010/10/
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Transformation of the sex pheromone signal in the noctuid moth agrotis ipsilon: From peripheral input to antennal lobe output Inra
Jarriault, D.; Gadenne, C.; Lucas, P.; Rospars, J.P.; Anton, S..
How information is transformed along synaptic processing stages is critically important to understand the neural basis of behavior in any sensory system. In moths, males rely on sex pheromone to find their mating partner. It is essential for a male to recognize the components present in a pheromone blend, their ratio, and the temporal pattern of the signal. To examine pheromone processing mechanisms at different levels of the olfactory pathway, we performed single-cell recordings of olfactory receptor neurons (ORNs) in the antenna and intracellular recordings of central neurons in the macroglomerular complex (MGC) of the antennal lobe of sexually mature Agrotis ipsilon male moths, using the same pheromone stimuli, stimulation protocol, and response...
Tipo: Journal Article Palavras-chave: AGROTIS IPSILON; LEPIDOPTERA; NOCTUIDAE; NEURONE; RECEPTEUR; OLFACTION; MITE; INSECTE; ARTHROPODA; HEXAPODA; NOCTUIDAE; LOBE ANTENNAIRE; ATTRACTIF SEXUEL; STIMILUS INTRACELLULAR RECORDING; MACROGLOMERULAR COMPLEX; MGC NEURON; OLFACTORY CODING; OLFACTORY RECEPTOR NEURON; SINGLE SENSILLUM RECORDING.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105ff8856a&uri=/notices/prodinra1/2010/11/
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Water taste transduction pathway is calcium dependent in Drosophila Inra
Meunier, N.; Marion-Poll, F.; Lucas, P..
In mammals, detection of osmolarity by the gustatory system was overlooked until recently. In insects, specific taste receptor neurons detect hypoosmotic stimuli and are commonly called “W” (water) cells. W cells are easy to access in vivo and represent a good model to study the transduction of hypoosmotic stimuli. Using pharmacological and genetic approaches in Drosophila, we show that tarsal W cell firing activity depends on the concentration of external calcium bathing the dendrite. This dependence was confirmed by the strong inhibition of W cell responses to hypoosmotic stimuli by lanthanum (IC50 = 8 nM), an ion known to inhibit calcium-permeable channels. Downstream, the transduction pathway likely involves calmodulin because calmodulin antagonists...
Tipo: Journal Article Palavras-chave: DROSOPHILA; ARTHROPODA; HEXAPODA; INSECTE; NEURONE; RECEPTEUR; STIMULUS HYPOOSMOTIQUE; GOUT; SENSATION; EAU; CALCIUM; CALMODULINE; OSMOLARITE  CALMODULIN; HYPOOSMOTIC; INSECT; OSMOLARITY; PKC; TRANSDUCTION.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20104d76cadf&uri=/notices/prodinra1/2010/10/
Registros recuperados: 14
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