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Detection and molecular characterization of Grapevine yellow speckle viroid 1 isolates infecting grapevines in Brazil. Repositório Alice
FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O..
Abstract Presently, Hop stunt viroid(HSVd) and Citrus exocortis viroid (CEVd) are the only viroids reported to infect grapevines (Vitis spp.) in Brazil, among the seven viroid species already reported infecting this host in other countries. All grapevine viroid diseases are graft-transmissible and can induce losses especiallywhenassociatedwithviruses.Theaimofthisworkwas to confirm infection by Grapevine yellow speckle viroid 1(GYSVd-1) in grapevine samples exhibiting yellow speckle symptoms in the leaves and in asymptomatic samples sequenced by next generation sequencing (NGS). The occurrence of this viroid in Brazil was further investigated in a second study. Total RNAs and dsRNAs were extracted from five symptomatic plants and 16 asymptomatic samples,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Next generation; Secondary structure; GYSVd-1; Vinho; Incidence; Vine; Hop stunt viroid; Citrus exocortis viroid.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1051941
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First report of Hop stunt viroid infecting Vitis gigas, V. flexuosa and Ampelopsis heterophylla. Repositório Alice
FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O..
Hop stunt viroid (HSVd) is one of the most common viroids that infect grapevine ( Vitis spp.) worldwide. Sixteen sequences of the HSVd genome were obtained from infected grapevines in Brazil by next generation sequencing (NGS). Multiple alignments of the sequences showed nucleotide identities ranging from 94.6% to 100%. This is the first report of HSVd infecting two wild grape species and Ampelopsis heterophylla . These HSVd isolates along with others from V. vinifera and V. labrusca were phylogenetically analyzed. Keywords: Next generation sequencing . HSVd . Incidence . Genetic variability . Vitis
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Next generation sequencing; HSVd; Grapevine; Genetic variability; Vitis Labrusca; Vitis Vinifera; Incidence; Vitis; Hop stunt viroid; Viroids; Brazil.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088430
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