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Agmatine inhibits hypoxia-induced TNF-alpha release from cultured retinal ganglion cells Biocell
Hong,Samin; Park,Kyoungsoo; Kim,Chan Yun; Seong,Gong Je.
The effect of hypoxia on the release of tumor necrosis factor-alpha (TNF-alpha) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-alpha in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100μM agmatine. The expression levels of TNF-alpha and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-alpha production in RGCs. Agmatine significantly reduced TNF-alpha level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or agmatine treatment. Our results show that agmatine inhibits the TNF-alpha production of RGCs in hypoxic...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Retinal ganglion cell; Tumor necrosis factor-alpha; Hypoxia; Agmatine.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200006
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Agricultural Drainage and Gulf Hypoxia: Economic Targeting of Farmland to Reduce Nitrogen Loads in a Minnesota Watershed AgEcon
Petrolia, Daniel R.; Gowda, Prasanna H.; Mulla, David J..
Agricultural nitrogen losses are the major contributor to nitrogen loads in the Mississippi River, and consequently, to the existence of a hypoxic, or "dead", zone in the Gulf of Mexico. Focusing on two small agricultural watersheds in southwestern Minnesota, simulation results from the Agricultural Drainage And Pesticide Management (ADAPT) model were combined with a linear-optimization model to evaluate the environmental and economic impact of alternative land-use policies for reducing nitrogen losses. Of particular importance was the study's explicit focus on agricultural subsurface (tile) drainage, which has been identified as the major pathway for agricultural nitrogen losses in the upper Midwest, and the use of drainage-focused abatement policies....
Tipo: Conference Paper or Presentation Palavras-chave: Drainage; Hypoxia; ADAPT; Nitrogen; Watershed; Environmental Economics and Policy.
Ano: 2005 URL: http://purl.umn.edu/19416
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Amino acid and ureide transport in the xylem of symbiotic soybean plants during short-term flooding of the root system in the presence of different sources of nitrogen Braz. J. Plant Physiol.
Thomas,André Luís; Sodek,Ladaslav.
The transport of organic N compounds to the shoot in the xylem sap of nodulated soybean plants was investigated in an attempt to better understand the changes in N metabolism under root hypoxia (first 5 days of flooding), with different sources of N in the medium. NO3- is beneficial for tolerance of plants to waterlogging, whereas other N sources such as NH4+ and NH4NO3, are not. Nevertheless, in the presence of NH4+ high levels of amino acids were transported in the xylem, consistent with its assimilation. Some increase in the transport of amino acids was also seen with NO3- nutrition during waterlogging, but not with N-free medium. Ureide transport in the xylem was severely reduced during waterlogging, consistent with impaired N2 fixation under these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Alanine; Hypoxia; N metabolism.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200010
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An Analysis of the Role of Tile-Drained Farmland Under Alternative Nitrogen Abatement Policies AgEcon
Petrolia, Daniel R.; Gowda, Prasanna H..
Agricultural nitrogen is a major contributor to Gulf of Mexico hypoxia, and research has shown that agricultural subsurface tile drainage is a major carrier of nitrogen from croplands to streams and rivers. This study compares the results of abating nitrogen under a retired-land minimization policy with those of a new revenue-maximizing policy, paying particular attention to the role of tile-drained land. Findings reveal the retirement-minimizing policy resulted in more tile-drained land being retired and less being fertilizer-managed than was optimal under the net-return maximizing policy. Also, it led to a greater economic burden being shouldered by tile-drained land. Under both cases, tile drainage dominated the abatement process.
Tipo: Journal Article Palavras-chave: Abatement; ADAPT; Drainage; Hypoxia; Nitrogen; Crop Production/Industries; Land Economics/Use.
Ano: 2006 URL: http://purl.umn.edu/8621
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An early-life hypoxia event has a long-term impact on protein digestion and growth in European sea bass juvenile. ArchiMer
Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy.
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined...
Tipo: Text Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf
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Anatomia ecológica de Sebastiania commersoniana (Baillon) Smith & Downs (Euphorbiaceae) submetida ao alagamento Rev. Bras. Bot.
KOLB,ROSANA M.; MEDRI,MOACYR E.; BIANCHINI,EDMILSON; PIMENTA,JOSÉ A.; GILONI,PATRICIA C.; CORREA,GERALDO T..
Em matas ciliares da Bacia Hidrográfica do Rio Tibagi, nas áreas sujeitas a inundações periódicas, é comum a ocorrência de S. commersoniana onde, geralmente, se torna a espécie dominante. A fim de elucidar alguns aspectos de tolerância ao alagamento, plantas dessa espécie foram mantidas na capacidade de campo e em solo alagado por dois meses. Após esse período, algumas plantas foram desalagadas para verificar o comportamento da espécie no período de reaeração. O alagamento induziu, em raízes, aumento de espaços intercelulares, redução na espessura do cilindro central e aumento na espessura do córtex. Plantas alagadas cresceram menos que as controle e desenvolveram lenticelas hipertróficas caulinares e algumas raízes adventícias. Plantas reaeradas retomaram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecological anatomy; Hypoxia; Sebastiania commersoniana.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041998000300010
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Anatomical and morphological modifications in response to flooding by six Cerrado tree species Acta Botanica
Oliveira,Adilson Serafim de; Ferreira,Cristiane Silva; Graciano-Ribeiro,Dalva; Franco,Augusto César.
Wetlands are common in the Cerrado (Brazilian savannas) biome, however flooding of these wetlands impairs growth and development of most plants. We evaluated flood tolerance of typical Cerrado trees. Seedlings of Aspidosperma macrocarpon (Apocynaceae), Tabebuia rosea (Bignoniaceae), Handroanthus chrysotrichus (Bignoniaceae), Myracrodruon urundeuva (Anacardiaceae), Kielmeyera coriacea (Calophyllaceae) and Copaifera langsdorffii (Fabaceae) were flooded up to the stem base for 30 days. Stems with cortical cracks, secondary aerenchyma and hypertrophic lenticels were observed in flooded plants of M. urundeuva,H. chrysotrichus and T. rosea while adventitious roots were formed in flooded plants of T. rosea and H. chrysotrichus. However, only T. rosea developed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aerenchyma; Biomass partitioning; Flood tolerance; Hypoxia; Water stress.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400478
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Angiotensin II type 1 receptor blockade partially attenuates hypoxia-induced pulmonary hypertension in newborn piglets: relationship with the nitrergic system BJMBR
Camelo Jr.,J.S.; Martins,A.R.; Rosa,E.; Ramos,S.G.; Hehre,D.; Bancalari,E.; Suguihara,C..
The objective of this study was to observe possible interactions between the renin-angiotensin and nitrergic systems in chronic hypoxia-induced pulmonary hypertension in newborn piglets. Thirteen chronically instrumented newborn piglets (6.3 ± 0.9 days; 2369 ± 491 g) were randomly assigned to receive saline (placebo, P) or the AT1 receptor (AT1-R) blocker L-158,809 (L) during 6 days of hypoxia (FiO2 = 0.12). During hypoxia, pulmonary arterial pressure (Ppa; P < 0.0001), pulmonary vascular resistance (PVR; P < 0.02) and the pulmonary to systemic vascular resistance ratio (PVR/SVR; P < 0.05) were significantly attenuated in the L (N = 7) group compared to the P group (N = 6). Western blot analysis of lung proteins showed a significant decrease of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newborn animals; Pulmonary hypertension; Angiotensin II; Angiotensin receptors; Hypoxia.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200012
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Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis Genet. Mol. Biol.
Almeida-Val,Vera Maria Fonseca; Oliveira,Alice Reis; Silva,Maria de Nazaré Paula da; Ferreira-Nozawa,Monica Stropa; Araújo,Roziete Mendes; Val,Adalberto Luis; Nozawa,Sérgio Ricardo.
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon; Fishes; Gene expression; RT-PCR; LDH-A; Hypoxia; Anoxia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200025
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Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil ArchiMer
Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy.
Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)...
Tipo: Text Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00347/45828/45916.pdf
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Assessing the long-term effect of exposure to dispersant-treated oil on fish health using hypoxia tolerance and temperature susceptibility as ecologically relevant biomarkers ArchiMer
Mauduit, Florian; Farrell, Anthony P.; Domenici, Paolo; Lacroix, Camille; Le Floch, Stephane; Lemaire, Pphilippe; Nicolas-kopec, Annabelle; Whittington, Mark; Le Bayon, Nicolas; Zambonino-infante, Jose-luis; Claireaux, Guy.
Consequences of exposure to polyaromatic hydrocarbons (PAH), toxic components of crude oil, on fish has been widely documented due to their ecological and economical importance. However, although forming a valuable and consistent body of knowledge, use of these data in spill response is limited. Objective of the present study was thus to facilitate the translation of published data into information of operational value. For this, we investigated the dose‐response relationship between dispersant‐treated oil exposure and ecologically‐relevant consequences by combining laboratory and field experiments. Effects were examined over almost a year using juveniles of the slow growing, commercially important European sea bass (Dicentrarchus labrax). Moreover, a...
Tipo: Text Palavras-chave: Oil spills; Polycyclic aromatic hydrocarbons (PAHs); Fish indices; Hypoxia; Temperature.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00455/56679/58438.pdf
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Carbonic anhydrase activity in the red blood cells of sea level and high altitude natives Biol. Res.
GAMBOA,JORGE; CACEDA,RICARDO; GAMBOA,ALFREDO; MONGE-C,CARLOS.
Red blood cell carbonic anhydrase (CA) activity has not been studied in high altitude natives. Because CA is an intraerythocytic enzyme and high altitude natives are polycythemic, it is important to know if the activity of CA per red cell volume is different from that of their sea level counterparts. Blood was collected from healthy subjects living in Lima (150m) and from twelve subjects from Cerro de Pasco (4330m), and hematocrit and carbonic anhydrase activity were measured. As expected, the high altitude natives had significantly higher hematocrits than the sea level controls (p=0.0002). No difference in the CA activity per milliliter of red cells was found between the two populations. There was no correlation between the hematocrit and CA activity.
Tipo: Journal article Palavras-chave: Carbonic anhydrase; Hypoxia; Polycythemia.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300006
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Cardioprotection against experimental myocardial ischemic injury using cornin BJMBR
Xu,Y.; Xu,Y.; Luan,H.; Jiang,Y.; Tian,X.; Zhang,S..
Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornin; Myocardial ischemia and reperfusion; CREB; Akt; Hypoxia.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200608
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Cardiovascular, respiratory and metabolic responses to temperature and hypoxia of the winter frog Rana catesbeiana BJMBR
The objective of the present study was to determine the effects of hypoxia and temperature on the cardiovascular and respiratory systems and plasma glucose levels of the winter bullfrog Rana catesbeiana. Body temperature was maintained at 10, 15, 25 and 35oC for measurements of breathing frequency, heart rate, arterial blood pressure, metabolic rate, plasma glucose levels, blood gases and acid-base status. Reducing body temperature from 35 to 10oC decreased (P&lt;0.001) heart rate (bpm) from 64.0 ± 3.1 (N = 5) to 12.5 ± 2.5 (N = 6) and blood pressure (mmHg) (P&lt;0.05) from 41.9 ± 2.1 (N = 5) to 33.1 ± 2.1 (N = 6), whereas no significant changes were observed under hypoxia. Hypoxia-induced changes in breathing frequency and acid-base status were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Temperature; Hypoxia; Rana; Breathing frequency; Blood pressure; Heart rate; Acid-base status; Hyperglycemia.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100019
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Changes in lactate dehydrogenase and malate dehydrogenase activities during hypoxia and after temperature acclimation in the armored fish, Rhinelepis strigosa (Siluriformes, Loricariidae) BJB
PANEPUCCI,L.; FERNANDES,M. N.; SANCHES,J. R.; RANTIN,F. T..
Lactate (LDH) and malate dehydrogenase (MDH) of white skeletal muscle of fishes acclimated to 20, 25 and 30°C and thereafter submitted to hypoxia were studied in different substrate concentrations. Significant differences for LDH and MDH of white muscle enzyme activities are described here for the first time in Rhinelepis strigosa of fishes acclimated to 20°C and submitted to hypoxia for six hours. LDH presented a significant decrease in enzyme affinity for pyruvate in acute hypoxia, for fishes acclimated to 20°C and an increase for fishes acclimated to 30°C.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypoxia; Acclimation temperature; Lactate dehydrogenase; Malate dehydrogenase; Armored fish; Rhinelepis strigosa.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200021
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Chemoreceptors and cardiovascular control in acute and chronic systemic hypoxia BJMBR
Marshall,J.M..
This review describes the ways in which the primary bradycardia and peripheral vasoconstriction evoked by selective stimulation of peripheral chemoreceptors can be modified by the secondary effects of a chemoreceptor-induced increase in ventilation. The evidence that strong stimulation of peripheral chemoreceptors can evoke the behavioural and cardiovascular components of the alerting or defence response which is characteristically evoked by novel or noxious stimuli is considered. The functional significance of all these influences in systemic hypoxia is then discussed with emphasis on the fact that these reflex changes can be overcome by the local effects of hypoxia: central neural hypoxia depresses ventilation, hypoxia acting on the heart causes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypoxia; Adenosine; Chemoreceptors; Vasodilatation; Noradrenaline.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700002
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Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma Biocell
Heon Ryu,Mi; Hee Park,Jeong; Eun Park,Ji; Chung,Jin; Hun Lee,Chang; Ryoun Park,Hae.
Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to cobalt chloride, suggesting that Akt phosphorylation by cobalt chloride is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypoxia; DNA synthesis; S-phase.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100003
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Comparative effects of long-term hypoxia on growth, feeding and oxygen consumption in juvenile turbot and European sea bass ArchiMer
Pichavant, Karine; Person, Jeannine; Le Bayon, Nicolas; Severe, Armelle; Le Roux, Annick; Boeuf, Gilles.
When juvenile turbot Scophthulmus maximus and sea bass Dicentrarchus labrax were fed to satiation, growth and food intake were depressed under hypoxia (3.2 +/- 0.3 and 4.5 +/- 0.2 mg O-2 l(-1)). However, no significant difference in growth was observed between fishes maintained in hypoxia and fed to satiation and fishes reared in normoxia (7.4 +/- 0.3 mg O-2 l(-1)) and fed restricted rations (same food intake of fishes at 3.2 mg O-2 l(-1)). Routine oxygen consumption of fishes fed to satiation was higher in normoxia than in hypoxia due to the decrease in food intake in the latter. Of the physiological parameters measured, no significant changes were observed in the two species maintained in hypoxia. This study confirms the significant interaction between...
Tipo: Text Palavras-chave: Oxygen consumption; Growth; Hypoxia; Sea bass; Turbot.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-617.pdf
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Conséquences à long terme d'une exposition précose à l'hypoxie sur la physiologie du bar européen (Dicentrarchus labrax) et de la sole commune (Solea solea) ArchiMer
Vanderplancke, Gwenaelle.
The dissolved oxygen in seawater has steadily declined in many coastal marine ecosystems in the last 50 years because of global warming and increasing coastal human activities. This decrease in oxygen in seawater causes episodes of hypoxia increasingly frequent and severe in coastal and estuarine areas. Some organisms may escape these environmental constraints by migration or dispersion. Others, with limited swimming capabilities must implement physiological regulation (phenotypic plasticity) to enable them to adapt and survive. This is particularly the case of marine fish larvae that are likely to be present in coastal nursery at the end of their development. It is now well established that the regulations implemented by organisms to cope with their...
Tipo: Text Palavras-chave: Hypoxie; Conditionnement; Programmation environnementale; Poissons marins; Changement climatique; Oxygène; Dicentrarchus labrax; Solea solea; Marine fish; Climate change; Oxygen; Hypoxia; Conditioning; Environmental programming.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00498/60938/64332.pdf
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Consommation d'oxygene de la crevette japonaise, Penaeus japonicus , en fonction de l'oxygenation du milieu: Effects de la temperature et de l'acclimatation a des conditions ambiantes hypoxiques ArchiMer
Truchot, J; Jouve, D.
Oxygen consumption of juvenile prawns, Penaeus japonicus , was measured during the day at 4 temperatures (23, 26, 30 and 33 degree C) and at various oxygenation levels. For inactive prawns burrowed into sand, respiratory metabolism was maintained almost constant in hypoxia above a critical oxygen pressure of about 30-40 Torr. Data analysis according to a hyperbolic model indicates that the capacity for metabolic regulation was optimal between 26 and 30 degree C. Prawns acclimated 1-2 weeks at 20 degree C in moderate hypoxia (P sub(O2) around 50-55 Torr) showed a reinforced regulation of oxygen consumption when compared to normoxic controls.
Tipo: Text Palavras-chave: Penaeus japonicus; Malacostraca; Juveniles; Respiration; Hypoxia; Dissolved oxygen; Temperature effects; Oxygen consumption; Crevettes Pénaeides; Température; Hypoxie; Métabolisme respiratoire.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1192.pdf
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