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Detection of a high prevalence of antibodies against Toxoplasma gondii in cattle in Northern and Midwestern Brazil Rev Salud Anim.
Barbosa da Silva,Jenevaldo; Nunes de Santana Castro,Gustavo; Nunes dos Santos,Priscilla; Henrique da Fonseca,Adivaldo; Henrique da Silva Lima,Danillo; dos Anjos Bomjardim,Henrique; dos Santos Belo Reis,Alessandra; de Oliveira Soares,Susiane; Diomedes Barbosa,José.
The aim of this study was to determine the prevalence of Toxoplasma gondii in cattle from the Northern and Midwestern regions of Brazil. Serum samples were collected from 1789 animals and tested by indirect enzyme-linked immunosorbent assay (ELISA). The overall prevalence of T. gondii was 83.40% (1492/1789). The prevalence rates of T. gondii-seropositive animals observed in the states of Pará, Tocantins and, Mato Grosso were 87.45%, 87.79% and 73.06%, respectively. The detection of high prevalence rates of T. gondii in cattle deserves special attention because they are the main source of high biological value protein for humans. This finding indicates the need for further studies on the risk that these animals may pose to public health.
Tipo: Journal article Palavras-chave: Cattle; ELISA; IFAT; Toxoplasma gondii.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100008
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Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle OAK
Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142
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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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Molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in Bahia State, Brazil. Repositório Alice
GONCALVES, I. N.; UZÊDA, R. S.; LACERDA, G. A.; MOREIRA, R. R. N.; ARAUJO, F. R.; OLIVEIRA, R. H. M. de; CORBELINE, L. G.; GODIM, L. F. P..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Toxoplasma gondii; Neospora caninum; Hammondia spp.; PCR; IFAT; Bioassay.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937536
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Preliminary Survey on Horse Serum Indirect Fluorescence Antibody Titers in Japan against Babesia equi and Babesia caballi (Onderstepoort strain) Antigen OAK
Suzuki, Naoyoshi; Igarashi, Ikuo; Abgaandorjiin, A.; Waal, D. T. De; Nagasawa, Hideyuki; Omata, Yoshitaka; Saito, Atsushi; Oyamada, Takashi.
Palavras-chave: Babesia caballi; Babesia equi; Sera diagnosis; IFAT; Survey.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/243
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Serodiagnosis of sleeping sickness in the Republic of the Congo : comparison of indirect immunofluorescent antibody test and card agglutination test IRD
Noireau, François; Lemesre, J.L.; Nzoukoudi, M.Y.; Louembet, M.T.; Gouteux, Jean-Paul; Frézil, Jean-Louis.
Tipo: Text Palavras-chave: MALADIE DU SOMMEIL; DEPISTAGE; EVALUATION; TEST; SEROLOGIE; CATT; IFAT.
Ano: 1988 URL: http://www.documentation.ird.fr/hor/fdi:25078
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Seroprevalence of Babesia caballi and Theileria equi in horses and mules from Northern Thailand OAK
Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X..
Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092
Registros recuperados: 7
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