Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 5
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
1H nuclear magnetic resonance spectroscopy-based studies of the metabolism of food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline by human intestinal microbiota Inra
Humblot, C.; Combourieu, B.; Väisänen, M.J.; Furet, J.P.; Delort, A.M.; Rabot, S..
2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a mutagenic/carcinogenic compound formed from meat and fish during cooking. Following ingestion, IQ is metabolized mainly by liver xenobiotic-metabolizing enzymes, but intestinal bacteria may also contribute to its biotransformation. The aim of this study was to investigate the metabolism of IQ by the human intestinal microbiota. Following incubation of IQ (200 microM) under anoxic conditions with 100-fold dilutions of stools freshly collected from three healthy volunteers, we quantified residual IQ by high-pressure liquid chromatography (HPLC) analysis and characterized the production of IQ metabolites by in situ (1)H nuclear magnetic resonance ((1)H-NMR) spectroscopic analysis of crude incubation media. In...
Tipo: Journal Article Palavras-chave: INTESTINAL MICROBIOTA; HUMAN; RAT; CARCINOGEN; MICROFLORE INTESTINALE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008cc87ce43&uri=/notices/prodinra1/2008/06/
Imagem não selecionada

Imprime registro no formato completo
Colonic microbiota signatures across five northern European countries Inra
Lay, C.; Rigottier-Gois, L.; Holmstrøm, K.; Rajilic, M.; Vaughan, E.; de Vos, V.M.; Collins, M.D.; Thiel, R.; Namsolleck, P.; Blaut, M.; Dore, J..
The composition of the colonic microbiota of 91 northern Europeans was characterized by fluorescent in situ hybridization using 18 phylogenetic probes. On average 75% of the bacteria were identified, and large interindividual variations were observed. Clostridium coccoides and Clostridium leptum were the dominant groups (28.0% and 25.2%), followed by the Bacteroides (8.5%). According to principal component analysis, no significant grouping with respect to geographic origin, age, or gender was observed.
Tipo: Journal Article Palavras-chave: FLUORESCENT IN SITU HYBRIDIZATION; PHYLOGENY; INTESTINAL MICROBIOTA; ADULT; CHILD; GENDER.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200810fe3a9b&uri=/notices/prodinra1/2008/06/
Imagem não selecionada

Imprime registro no formato completo
Constitutive delivery of bovine beta-lactoglobulin to the digestive tracts of gnotobiotic mice by engineered Lactobacillus casei Inra
Hazebrouck, S.; Oozeer, R.; Adel-Patient, K.; Langella, P.; Rabot, S.; Wal, J.M.; Corthier, G..
The gut microbiota is critical for maturation of the immune system. Recent evidence suggests that early establishment of lactobacilli in the intestinal microbiota, during neonatal colonization or by probiotic supplementation, could prevent the development of allergic disorders. Postnatal maturation of the gut immune system with allergen-producing lactobacilli colonizing the digestive tract could then affect the development of further allergic sensitization. In this paper, we describe construction of a recombinant Lactobacillus casei strain that can constitutively deliver bovine beta-lactoglobulin (BLG), a major cow's milk allergen, to the guts of gnotobiotic mice. The blg gene was inserted into the L. casei chromosome downstream of an endogenous promoter....
Tipo: Journal Article Palavras-chave: BOVINE BETA-LACTOGLOBULIN; ALLERGEN; PROBIOTIC; INTESTINAL MICROBIOTA; GUT IMMUNE SYSTEM; DIGESTIVE TRACT.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e9e33f45&uri=/notices/prodinra1/2008/06/
Imagem não selecionada

Imprime registro no formato completo
Differential activities of four Lactobacillus casei promoters during bacterial transit through the gastrointestinal tracts of human-microbiota-associated mice Inra
Oozeer, R.; Furet, J.P.; Goupil-Feuillerat, N.; Anba, J.; Mengaud, J.; Corthier, G..
In a previous study using fusion of the deregulated lactose promoter lacTp* and reporter genes, we suggested that Lactobacillus casei could initiate de novo protein synthesis during intestinal transit. In order to confirm this finding and extend it to other promoters, we adopted a reverse transcriptase quantitative PCR (RT-QPCR) approach combined with a transcriptional fusion system consisting of luciferase genes under the control of four promoters (ccpA, dlt, ldh, and lacT*) from L. casei DN-114 001. Promoter expression was monitored during cell growth, and variable luciferase activities were detected. In 3-day cultures, all the genetically modified strains survived but without exhibiting luciferase activity. Luciferase mRNA levels determined by RT-QPCR...
Tipo: Journal Article Palavras-chave: HUMAN; MOUSE; GENE EXPRESSION; INTESTINAL MICROBIOTA; FECES; RT-QPCR; PROBIOTIC; GASTROINTESTINAL TRACT.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008ec5c541b&uri=/notices/prodinra1/2008/06/
Imagem não selecionada

Imprime registro no formato completo
Search for localized dysbiosis in Crohn's disease ulcerations by temporal temperature gradient gel electrophoresis of 16S rRNA Inra
Seksik, P.; Lepage, P.; de la Cochetière, M.F.; Bourreille, A.; Sutren, M.; Galmiche, J.P.; Dore, J.; Marteau, P..
The mucosa-associated microbiota lining the gut epithelium might play a central role in the activation and/or perpetuation of mucosal inflammation in Crohn's disease (CD). We sought for localized dysbiosis by comparing the biodiversity and composition of the microbiotas in ulcerated and nonulcerated mucosal samples from patients with CD. Biopsy samples (n = 75) of ulcerated and adjacent nonulcerated mucosa were collected during colonoscopy from 15 patients, from the ileum, right colon, left colon, and rectum. Temporal temperature gradient gel electrophoresis (TTGE) of bacterial 16S rRNAs was used to evaluate the dominant bacterial species. TTGE profiles were compared using software that calculates similarity percentages. For a given patient, average...
Tipo: Journal Article Palavras-chave: CROHN'S DISEASE; INTESTINAL MUCOSA; INTESTINAL MICROBIOTA; TTGE; 16S rRNA.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200880737e8&uri=/notices/prodinra1/2008/06/
Registros recuperados: 5
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional