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Registros recuperados: 11
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Caractérisation et analyse de l'expression des pénaeidines, peptides antimicrobiens isolés chez la crevette pénéide Penaeus vannamei (Crustacea, Decapoda) ArchiMer
Destoumieux, Delphine.
Haemocytes are the key elements of a complex defence system on which crustacean immunity is largely based. We report here for the first time in a crustacean that haemocytes are involved in the synthesis of antimicrobial peptides similar to those described in other arthropod classes. These molecules isolated from the penaeid shrimp Penaeus vannamei are members of an homogeneous farnily which differ from the antimicrobial peptides previously described in that they combine in a single molecule a proline-rich arninoterminal domain, and a cyclic carboxy-terminal domain containing three intramolecular disulphide bridges. In addition, penaeidins bear post-translational modifications at their arnino- and carboxy-termini. Five molecules of the penaeidin family were...
Tipo: Text Palavras-chave: Crustacé; Crevette; Immunité; Hémocyte; Peptide; Antimicrobien; Cationique.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00440/55111/56564.pdf
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Contribution à l'étude de l'ontogenèse du système immunitaire chez l'huître creuse Crassostrea gigas ArchiMer
Tirape Bajana, Ana.
The interest for the understanding of the immune mechanisms in the oyster, Crassotrea gigas has been considerably increased these last years, mainly because of economie losses due to diseases which affected the continuity of this species. The studies of these mechanisms contributes to a better understanding of the differential susceptibilities observed in this species against various pathogens. In the present study, our  goal was to define the ontogenesis of immune system in C. gigas during the development, by functional genomic approaches. The studies have been focalized on the analyses of expression and localization of effectors previously identified in adult oysters as implicated in immunity. We have shown, in particular, that hemocytes appear in the...
Tipo: Text Palavras-chave: Invertébré; Mollusque bivalve; Huître; Cra; Immunité; Hémocytes; Hématopoïèse; Développement; Ontogenèse; Expression des gènes; Q-PCR; SSH; Invertebrate; Bivalve Mollusk; Oyster; Crassostrea gigas; Immunity; Hemocytes; Hematopoiesis; Development; Ontogenesis; Gene expression; Q-PCR; SSH.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00440/55113/56565.pdf
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Ecologie du système hôte - parasite, juvéniles G0 de sole (Solea solea) - métacercaires de Digènes : dynamique et effets de l'infestation ArchiMer
Durieux, Eric.
Coastal areas, such as shallow water bays and estuaries, serve as nurseries for juveniles of many marine fishes and especially flatfishes. Parasites play an important role in these marine ecosystems, but it remains poorly studied. Little attention has been paid to parasitism as a potential factor of variation in flatfish juveniles biological performances in these habitats. Sole juveniles, Solea solea (Pleuronectiformes, Soleidae), that is a species of primary importance for fisheries, are infected by digenean metacercariae. Thus, the aims of this PhD thesis were: i) to determine the spatio-temporal dynamics of the digenean metacercariae infection of 0-group sole juveniles at different scales; ii) to estimate the effects of this infection at different...
Tipo: Text Palavras-chave: North East Atlantic; Mussel farming; Biological tags; Behaviour; Condition indices; Growth; Immunity; Parasitism; Flatfish; Nurseries; Atlantique Nord Est; Mytiliculture; Marqueurs biologiques; Comportement; Indices de condition; Croissance; Immunité; Parasitisme; Pleuronectiformes; Nourriceries.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-2933.pdf
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Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens ArchiMer
Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf
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Etude expérimentale de l'influence de deux stress environnementaux sur les défenses immunitaires et la résistance à la vibriose de la crevette Penaeus stylirostris ArchiMer
Gangnery, Aline.
Cette étude a pour objectif de mesurer chez la crevette Penaeus stylirostris l'effet de deux stress environnementaux, ammoniaque et hypoxie, sur les défenses immunitaires (hémocytes et prophénoloxydase) et la résistance à l' infection par la bactérie Vibrio penaeicidae. Les crevettes, sélectionnées en stade C du cycle de mue, ont été soumises pendant 24 heures à plus ieurs doses d'ammoniaque N.NH3 ( 1, 1 .5, 2, 2.5 et 3 mg/l) ou placées en situation d' hypoxie (1 ppm vs 6ppm en normoxie). L'effet du stress a été évalué à 24 h par la mesure de la capacité osmorégulatrice ainsi que les paramètres immunitaires. Les résultats montrent que l'ammoni aque à 3 mg N.NH3/I induit une diminution significative du nombre total des hémocytes, cependant que l'activité de...
Tipo: Text Palavras-chave: Penaeus stylirostris; Vibrio penaeicidae; Immunité; Hémocyte; Prophénoloxydase; Infection bactérienne; Capacité osmorégulatrice; Ammoniaque; Hypoxie.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00441/55273/56767.pdf
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Implication des peptides antimicrobiens dans l'immunité innée de la moule, Mytilus galloprovincialis ArchiMer
Mitta, Guillaume.
Les mécanismes immunitaires innés des vertébrés sont une mosaïque de mécanismes ancestraux partagés avec des animaux évolutivement plus primitifs. Les peptides antimicrobiens sont une de ces composantes conservées au travers du règne animal. En effet, ils participent vraisemblablement chez tous les métazoaires aux processi d'immunité innée. Toutefois, leurs implications dans la réponse anti-infectieuse peuvent être assez différentes. L'objet de cette thèse était de comprendre comment ces peptides participaient aux processi de défense de la moule. Une première phase de caractérisation biochimique de telles molécules avait été entreprise chez la moule, Mytilus galloprovincialis, dans le cadre de la thèse de Florence Hubert. Notre travail a consisté (i) à...
Tipo: Text Palavras-chave: Immunité; Peptides; Antibiotiques; Moule; Défensine.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00439/55101/56547.pdf
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Influence de l'environnement trophique de l'élevage en biofloc sur les performances physiologiques de la crevette Litopenaeus stylirostris : Étude de paramètres de la nutrition, de l'immunité et de la reproduction ArchiMer
Cardona, Emilie.
Biofloc is an intensive rearing system with zero or minimal water exchange where a diverse population of microorganisms (microalgae, zooplankton and bacteria) develops in association with organic matter to form the floc particles. These particles play the double role of biological filter and dietary supplement. This dissertation aims to better understand the process of this rearing system and its interactions with the Litopenaeus stylirostris shrimp. Two specific objectives were integrated within the framework of this general objective: (i) to measure the production gain from biofloc rearing and (ii) to study the interaction between biofloc environment and shrimp and to assess its role on production performances of shrimps. Thus, our results show...
Tipo: Text Palavras-chave: Biofloc; Crevette; Nutrition; Stress oxydant; Défenses antioxydantes; Immunité; Reproduction; Oeufs; Larves; Bactéries; Microbiote; Biofloc; Shrimp; Nutrition; Oxidative stress; Antioxidant defences; Immunity; Reproduction; Eggs; Larvae; Bacteria; Microbiota.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00272/38294/36492.pdf
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Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires ArchiMer
Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (>17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf
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Journées conchylicoles 1997 Ifremer Nantes les 18 et 19 mars 1997 ArchiMer
IFREMER.
Ce rapport synthétise les communications faites lors des "Journées conchylicoles", sur les travaux scientifiques de l'Ifremer en soutien aux activités conchylicoles
Tipo: Text Palavras-chave: Immunité; Ecloserie; Génétique; Pathologie; Ecophysiologie; Système d'élevage; Claires ostréicoles.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00200/31113/29524.pdf
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Modulation des paramètres hémocytaires par la nutrition de l'huitre creuse Crassostrea gigas. Implication dans les mortalités estivales ArchiMer
Delaporte, Maryse.
In the context of Pacific oyster C. gigas summer mortality, the present study demonstrated that the reproductive effort reduced not only the energetic parameters (carbohydrates and CEA) but also the immune parameters (concentration, phagocytosis and adhesive capacity of hemocytes). Increasing food supply from 4% to 12% (of oyster dry weight) did not counteract this phenomena as most of the additional energy was dedicated to the gametogenesis processes. However, after the gametogenesis, the 12% diet clearly favored energy storage restoration and hemocyte production. Hemocyte parameters were also modulated by the food quality. Indeed, hemocyte concentration tended to increase 20:5n-3 and/or 20:4n-6 were supplied by micro-algae or artificial diets. When these...
Tipo: Text Palavras-chave: Summer mortalities; Flow cytometer; Immunity; Oxidative burst; Phagocytosis; Biochemical composition hemocyte parameters; Essential fatty acids; Nutrition; Crassostrea gigas; Mortalités estivales; Cytomètre de flux; Immunité; Poussée respiratoire; Phagocytose; Réponses hémocytaires; Composition biochimique; Acides gras essentiels; Nutrition; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-357.pdf
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Mortalités de moules bleues dans les Pertuis Charentais: description et facteurs liés – MORBLEU ArchiMer
Travers, Marie-agnes; Pepin, Jean-francois; Soletchnik, Patrick; Guesdon, Stephane; Le Moine, Olivier.
Depuis 2014, des surmortalités anormales affectent les cheptels mytilicoles tant adultes que juvéniles avec des mortalités massives pouvant atteindre 100% (Béchemin et al. 2015). Les objectifs généraux de ce projet sont d’identifier des facteurs potentiellement corrélés avec les mortalités de moules observées dans les Pertuis Charentais: facteurs environnementaux biotiques, abiotiques et hydrodynamiques) et facteurs intrinsèques aux animaux (traits d'histoire de vie, qualité cytogénétique et physiologie). Après une introduction bibliographique complète, différentes questions sont ici abordées. 1. Historique des mortalités 2014-2015 ? Grâce { différentes sources d’information nous avons pu retracer les évènements de mortalités de 2014 et 2015 et valider...
Tipo: Text Palavras-chave: Moules; Mytilus edulis; Fluctuations environnementales; Connectivité hydrodynamique; Populations; Qualité cytogénétique; Immunité; Physiologie.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43539/43096.pdf
Registros recuperados: 11
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