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Registros recuperados: 14
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A case study of disseminated histoplasmosis linked to common variable immunodeficiency BJID
Rachid,Acir; Rezende,Lucila Stange; Moura,Suzelle Freitas de; Loffy,Paulo C.; Magalhães,Francisco Luiz Gomide M..
Histoplasma capsulatum, a ground fungus, can infect humans, normally in endemic areas; the resulting disease can be asymptomatic or it can have a benign development, but in rare cases it can develop into a serious clinical condition and can even be fatal. Its most characteristic initial location is in the lungs, resembling tuberculosis, often accompanied by mediastinitis and an exuberant fibrotic response. The spread of this infection can be caused by the concomitance of another illness that alters the immunological balance. Sometimes such an association is not clear. Therefore, disseminated histoplasmosis is defined as a clinical condition where the fungus is present in more than one location. Common variable immunodeficiency is characterized by a...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Disseminated histoplasmosis; Common variable immunodeficiency; Immunoglobulins.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000400007
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A new methodology for polyvalent intravenous immunoglobulin solution production with a two-stage process of viral inactivation BJPS
Lucena,Antônio Edson de Souza; Sampaio,Divaldo de Almeida; Silva,Ednaldo Rosas da; Paiva,Virgínia Florêncio de; Santiago,Ana Cláudia; Leite,Ana Cristina Lima.
Highly purified intravenous immunoglobulin G concentrate (IV IgG) was produced with the use of polyethylene glycol associated to a single-stage precipitation by ethanol, instead of the classic Cohn-Oncley process, which employs cold alcohol as the precipitating agent, in a three-stage process. Precipitation of crude fraction containing more than 95% of immunoglobulin G was performed by liquid chromatography with a cation exchanger, CM-Sepharose, as a stationary phase. During the process, the product was subjected to two-stage viral inactivation. The first stage was performed by the action of sodium caprylate, 30 mM at pH 5.1+/- 0.1, and the second stage was performed by the action of a solvent-detergent mixture. The finished product was formulated at 5%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood derivatives; Immunoglobulins; Polyethylene glycol; Ultrafiltration; Ion exchange chromatography.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400020
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Antibody and cytokine serum levels in patients subjected to anti-rabies prophylaxis with serum-vaccination J. Venom. Anim. Toxins incl. Trop. Dis.
Ayres,J. A.; Barraviera,B.; Calvi,S. A.; Carvalho,N. R.; Peraçoli,M. T. S..
Rabies is considered a fatal disease once clinical symptoms have developed. The aim of this study was to evaluate epidemiological aspects and immune response in patients attacked by domestic and wild animals and subjected to post-exposure rabies treatment with equine serum and associated vaccine. Thirty-three patients were evaluated; they were between 13 and 65 years old, 75.8% were male and 24.2% female, and from the Botucatu neighborhood. Twenty healthy control individuals with the same age range were also studied. Specific antibodies to equine immunoglobulins and IFN-gamma, IL-2, IL-4, and IL-10 production were evaluated by ELISA. IgM, IgE, IgG and subclasses, and rabies virus antibodies serum levels were determined by nephelometry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Anti-rabies prophylaxis; Immunoglobulins; Cytokines.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300008
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Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental. Repositório Alice
RODRIGUES, R. O.; HERRMANN, G. P.; HEINEMANN, M. B.; LAGE, A. P.; LOPES, L. B.; MOREIRA, E. C..
Resumo: O presente estudo avaliou a indução da produção de anticorpos contra Leptospira spp.por dez bacterinas,sendo nove polivalentes e uma monovalente experimental para a sorovariedade Hardjo amostra Norma. A concentração celular foi controlada e utilizou-se adjuvante de emulsão óleo em água. Um ensaio imunoenzimático (ELISA) indireto foi desenvolvido utilizando-se conjugado anti-IgG total para mensurar os níveis de anticorpos da classe IgG conferido pelas bacterinas utilizando três amostras diferentes: Hardjoprajitino, Norma e Hardjobovis. Paralelamente foi utilizado também o Teste de Soroaglutinação Microscópica (SAM) para mensurar os níveis de anticorpos contra as mesmas amostras. Encontraram-se títulos variáveis entre as bacterinas de acordo com o...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Leptospira; Bovino; Vacina; Imunoglobulina; Cattle; Vaccines; Immunoglobulins.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/904138
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Economic evaluation of snake antivenom production in the public system J. Venom. Anim. Toxins incl. Trop. Dis.
Morais,V.; Massaldi,H..
Snake antivenom, an expensive animal product, is presently the only effective treatment for the consequences of snakebite. In Latin America, antivenoms are mainly produced by public institutions with frequent shortages of the necessary supply. Here, we present an economical analysis of the factors affecting production cost, assuming a basic processing batch of 100 L hyperimmune plasma. Three annual production volumes were considered for two typical production technologies. The components of cost were classified as fixed, variable and semi-variable. We found that in all stages of production, fixed cost represents the major contribution to total cost, and is given essentially by manpower cost, particularly for low production volumes. Our estimation shows...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antivenom; Economical cost; Immunoglobulins; Equine plasma; Serum production.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300012
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Efeito do fornecimento de colostro caprino na transferência de imunidade passiva e no estresse de cordeiros. Repositório Alice
MELO, F. C. C. de..
Resumo: O presente trabalho teve como objetivo avaliar a influência do manejo de fornecimento de colostro de cabra na aquisição de imunidade passiva de cordeiros até o desmame, mediante a determinação da proteína total, albumina, globulina e gamaglutamiltransferase (GGT) como possíveis indicadores de transferência de imunidade passiva, bem como a dinâmica dos perfis bioquímicos de glicose, creatinina, uréia, cortisol. Foram utilizados 20 cordeiros, machos e fêmeas, com graus de sangue variados, nascidos de mães pluríparas, no qual 10 cordeiros receberam um fornecimento adicional de 10% do peso vivo de colostro caprino termizado, após o parto, dividido em quatro porções e 10 cordeiros permaneceram com a mãe e se alimentaram com colostro ad libitum. Os...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Caprino; Cabra; Cordeiro; Colostro; Imunoglobulina; Cortisol; Soro de leite; Proteína; Goats; Lambs; Colostrum; Hydrocortisone; Kids; Whey; Immunoglobulins; Proteins.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/997242
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Effect of Embryo Thermal Stimulation on the Serum Levels of Immunoglobulins and Corticosterone, and Organ Histopathology of 1 day-old Broilers Rev. Bras. Ciênc. Avic.
Flores,F; Nääs,IA; Garcia,RG; Quinteiro,WMQ; Souza,LI.
ABSTRACT Embryo thermal stimulation has been studied as a means to promote epigenetic changes and to improve broiler health and performance. This study aimed at evaluating immunoglobulin (IgM, IgG, and IgA) titers, serum corticosterone levels, and organ integrity of day-old male Cobb(r) and Ross(r) broilers. Embryos were submitted to hot and cold stimuli (thermal stimulation) on days 14-18 of incubation. The thermal manipulation treatments did not affect immunoglobulin titers of 1 day-old Cobb(r) or Ross(r) chicks, and caused severe bursal lesions (scores 3 and 4) in both strains. However, serum corticosterone levels of Cobb(r) chicks were higher when embryos were submitted to hot stimulus (+1.39ºC) compared with cold stimulus (+36 ºC). Serum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corticosterone; Caloric stress; Immunoglobulins; Termmanipulation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600071
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Effects of Methionine Deficiency on the B Lymphocyte and Immunoglobulins of Cecal Tonsil in Cobb Broilers Rev. Bras. Ciênc. Avic.
Zhu,M; Ruan,T; Zeng,Q; Wu,B.
ABSTRACT The present study was conducted on one-day-old broilers to investigate the effects of methionine deficiency on B lymphocytes and immunoglobulins (sIgA, IgA, IgG, IgM) in the cecal tonsil of Cobb broiler chicken. Methods including immunohistochemistry (IHC), western blot (WB), quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) and Enzyme-Linked Immuno Sorbent Assay (ELISA) were used, and the study lasted for 6 weeks. It was found that the IgA+ B lymphocytes mainly existed in the diffuse lymphoid tissues and lymphoid follicles of the cecal tonsil. The data indicated that the number of IgA+ B lymphocytes was decreased in the methionine deficiency group, and the WB and qRT-PCR results suggested that the protein and mRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B lymphocyte; Cecal tonsil; Immunoglobulins; Methionine deficiency.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400319
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Estudo da dinâmica da absorção protéica do colostro em crias Moxotó e Saanen no Ceará. Repositório Alice
ELOY, A. M. X.; ANDRADE, M. L. R. de; PINHEIRO, R. R.; SILVA, N. M. M. da; BRITO, R. L. L.; SANTIAGO, L. B.; LOBO, R. N. B.; FURTADO, J. R..
Investigou-se o comportamento das proteínas totais e de suas frações (albumina, globulina e gamaglobulina) durante 22 dias pós-parto, em fêmeas das raças Moxotó e Saanen, e de suas crias que receberam colostro termizado. As proteínas totais no colostro e no soro das cabras Moxotó e Saanen não apresentaram diferença (P>0,05) (6,65±3,06; 6,34±0,54 g/dL vs 6,23±4,49; 6,61±0,53 g/dL), respectivamente, entre as raças, enquanto suas respectivas crias diferiram (P<0,05)(6,62±0,45 vs 5,15±0,76 g/dL). Não houve diferença (P>0,05) entre a albumina no colostro e no soro (2,52±0,31; 2,87±0,18 vs 2,31±0,19; 3,68±0,76 g/dL) das fêmeas Moxotó e Saanen, respectivamente, como também no soro de suas respectivas crias (P>0,05)(2,54±0,18; 2,88±0,99 g/dL). A...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Imunidade passiva; Raça Moxotó; Raça Saanen; Caprino; Imunoglobulina; Colostro; Soro-sanguíneo; Raça Moxotó; Anticorpo; Goats; Passive immunity; Immunoglobulins; Colostrum; Antibody formation.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/972696
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Identification of enteropathogens from buffalo calves with and without diarrhoea in the Ribeira Valley, State of São Paulo, Brazil Braz. J. Vet. Res. Anim. Sci.
RIBEIRO,Márcio Garcia; LANGONI,Hélio; JEREZ,José Antônio; LEITE,Domingos da Silva; FERREIRA,Fernando; GENNARI,Solange Maria.
Faecal samples of 106 buffalo calves aged 3 to 45 days were collected sequentially, once a week, during six weeks for parasitologic examination. For bacteriologic and virologic exams, faecal samples were collected from all diarrhoeic and the same amount on non-diarrhoeic calves. Blood samples from calves were collected at 3 to 10 days of age for serum IgG determination. Eimeria spp, Strongyloides papillosus and Toxocara vitulorum were the most common parasites. E. coli, Enterobacter cloacae and Klebsiella pneumoniae were the enteropathogens most frequently found (alone or in association with other microrganisms). Heat-stable (STa) enterotoxin was detected in 5 out of 34 samples of E. coli isolated from diarrhoeic calves. Among the antimicrobials tested...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhoea; Buffalo calves; Immunoglobulins.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200013
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Immunoglobulin production is impaired in protein-deprived mice and can be restored by dietary protein supplementation BJMBR
Amaral,J.F.; Foschetti,D.A.; Assis,F.A.; Menezes,J.S.; Vaz,N.M.; Faria,A.M.C..
Most contacts with food protein and microbiota antigens occur at the level of the gut mucosa. In animal models where this natural stimulation is absent, such as germ-free and antigen-free mice, the gut-associated lymphoid tissue (GALT) and systemic immunological activities are underdeveloped. We have shown that food proteins play a critical role in the full development of the immune system. C57BL/6 mice weaned to a diet in which intact proteins are replaced by equivalent amounts of amino acids (Aa diet) have a poorly developed GALT as well as low levels of serum immunoglobulins (total Ig, IgG, and IgA, but not IgM). In the present study, we evaluated whether the introduction of a protein-containing diet in 10 adult Aa-fed C57BL/6 mice could restore their...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Food proteins; Immunoglobulins; Maturation; Mucosa.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200009
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Renal involvement in canine leishmaniasis: a morphological and immunohistochemical study Arq. Bras. Med. Vet. Zootec.
Soares,M.J.V.; Moraes,J.R.E.; Moraes,F.R..
IgG and IgM deposits in kidneys of dogs with visceral leishmaniasis (VL) were studied in 25 symptomatic dogs (case) and 15 asymptomatic dogs (control) by an immunohistochemical method. All tested dogs were positive for VL by polymerase chain reaction, enzyme-linked immunosorbent assay, and indirect immunofluorescence test. Kidney fragments were submitted to immunohistochemical reaction. Many morphological patterns of distribution of subendothelial granules were identified for IgG and IgM in glomerular capillaries: global, segmental, diffuse, or focal. Intensity of immunohistochemical reaction to IgG was not significantly different when comparing the symptomatic and the asymptomatic animal groups by Fisher's exact test. IgM reactions were significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Leishmania; Kidneys; Glomerulonephritis; Immunoglobulins.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400004
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Teste de conglutinação rápida para diagnóstico de anticorpos contra Babesia bovis. Repositório Alice
MADRUGA, C.R.; KESSLER, R.H.; MIGUITA, M..
Neste trabalho objetivou-se desenvolver e avaliar um teste de conglutinacao rapida para diagnostico de anticorpos contra Babesia bovis (TCR - B.bovis), com desempenho similar ao do teste da imonofluorescencia indireta. O TCR - B.bovis apresentou sensibilidade de 95,7% especificidade de 97,6% e precisao de 96,5%. Os mesmos parametros para imunofluorescencia indireta (IFI) foram respectivamente 99,1%, 99,7% e 99,4%. O TCR demonstrou ter capacidade de determinar imunoglobulinas especificas anti B.bovis tao cedo quanto a IFI, apos infeccao experimental com este hemoprotozoario. As reacoes cruzadas do TCR - B.bovis com soros de animais infectados com Babesia bigemina ocorreram em 4% dos soros. O antigeno para TCR produzido com hemacias nao parasitadas...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Imunofluorescencia; Imunoglobulinas; Hemoprotozoario; Immunofluorescent antibodies; Immunoglobulins; Hemoprotozoans.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/99151
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Transfer of passive immunity and serum proteinogram in the first six months of life of Criollo Lageano and Black and White Holstein calves. Repositório Alice
TEIXEIRA, W. T.; FONTEQUE, G. V.; RAMOS, A. F.; MARIANTE, A. da S.; EGITO, A. A. do; MARTINS, V. M. V..
O objetivo do presente trabalho foi verificar e comparar a transferência de imunidade passiva e o proteinograma em bezerros da raça Crioula Lageana (CL) e Holandesa Preta e Branca (HPB).
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Immunoglobulins; Bovine; Serum proteins; Native breeds.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937229
Registros recuperados: 14
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