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| Registros recuperados: 136 | |
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| Bastías,Adriana; Almada,Rubén; Rojas,Pamela; Donoso,José Manuel; Hinrichsen,Patricio; Sagredo,Boris. |
| A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172... |
| Tipo: Journal article |
Palavras-chave: Development; In vitro culture; Hase change; Rejuvenation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009 |
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| MACIEL, S. de A.. |
| As espécies Piper hispidinervum e Piper aduncum, conhecidas popularmente como pimenta longa e pimenta de macaco, respectivamente, apresentam compostos metabólicos secundários como safrol e dilapiol em seus óleos essenciais. Devido ao interesse do mercado químico, a produção de mudas em larga escala tem sido requerida por meio da técnica de cultura de tecidos de vegetais. Porém, a alta mortalidade de plantas durante a transição do ambiente in vitro para o ex vitro, em consequência de desordens anatômicas, morfológicas e fisiológicas, tem criado obstáculos para o uso generalizado dessa técnica. O trabalho teve como objetivo comparar aspectos anatômicos, relacionados a estruturas fisiológicas das folhas de P. hispidinervum e P. aduncum propagadas in vitro, in... |
| Tipo: Tese/dissertação (ALICE) |
Palavras-chave: Cultura ex vitro; Ex vitro culture; Cultura in vivo; Leaf analysis; Análisis foliares; Micropropagación; Estudios in vivo; Pimenta Longa; Piper Hispidinervum; Pimenta de Macaco; Análise Foliar; Micropropagação; Cultura In Vitro; Piper longum; Piper aduncum; Micropropagation; In vitro culture; In vivo studies. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093359 |
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| Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette. |
| ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292 |
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| LEDO, A. da S.; LAMEIRA, O. A.; BENBADIS, A. K.; MENEZES, I. C. de; OLIVEIRA, M. do S. P. de; LEDO, C. A. da S.. |
| O objetivo do presente trabalho foi avaliar a eficiência da adição de antioxidantes no meio de cultura e o efeito nos estádios de desenvolvimento de inflorescências de açaizeiro (Euterpe oleracea Mart.), sobre a intensidade de oxidação de explantes cultivados in vitro. As atividades foram realizadas no Laboratório de Recursos Genéticos e Biotecnologia da Embrapa Amazônia Oriental, Belém (PA). Os segmentos de ráquilas, obtidos de inflorescências de açaizeiro previamente desinfestadas, foram inoculados em tubos de ensaio contendo meio de cultura de MS (Murashige & Skoog. 1962), sólido e líquido, com 3 % de sacarose, suplementado com diferentes antioxidantes. As culturas foram mantidas em sala de crescimento sob ausência total de luz. Aos 15 dias após a... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Açaí de touceira; Açaí da várzea; Açaí verdadeiro; Carvão ativado; Micropropagação; Açaí; Euterpe oleracea; Cultura in vitro; Explante; Inflorescência; Antioxidante; Vitamina C; Micropropagation; In vitro culture; Explants; Inflorescences; Antioxidants; Ascorbic acid; Activated carbon; Polyvinylpyrrolidone; Micropropagación; Cultivo in vitro; Explantes; Inflorescencias; Antioxidantes; Ácido ascórbico; Carbono activado; Polivinilpirrolidona. |
| Ano: 2001 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1053098 |
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| Castro,Ana Hortência Fonsêca; Braga,Karina de Queiroz; Sousa,Fernanda Marinez de; Coimbra,Mairon César; Chagas,Rafael César Russo. |
| ABSTRACT This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 µM) and BAP (0; 4.44; 8.88; 17.75 µM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; Medicinal plant; In vitro culture; Secondary metabolites. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100143 |
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| Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo. |
| ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203 |
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| Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa. |
| The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012 |
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| Dias, Maria Inês; Barros, Lillian; Sousa, Maria João; Ferreira, Isabel C.F.R.. |
| Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics, flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity, reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity... |
| Tipo: Article |
Palavras-chave: Antioxidants; Coriandrum sativum; In vitro culture; Lipid peroxidation inhibition; Micropropagation; Radical scavenging activity. |
| Ano: 2011 |
URL: http://hdl.handle.net/10198/6242 |
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| PEREIRA, J.E.S.; FORTES, G.R. de L.; SILVA, J.B.. |
| O objetivo deste trabalho foi otimizar o crescimento de plantas micropropagadas do porta-enxerto de macieira 'Marubakaido' (Malus prunifolia) em casa de vegetação, por meio da aplicação de ácido giberélico (AG3) por uma, duas, ou três vezes, em intervalos semanais. As concentrações testadas foram: 0, 50, 100, 200, 400, 800 e 1.600 mg L-1. O crescimento das plantas foi avaliado quinzenalmente, por um período de dois meses. O comprimento dos entrenós e a matéria seca da parte aérea das plantas também foram avaliados no final do experimento. Três aplicações de AG3 na concentração de 800 mg L-1 foi o tratamento que proporcionou a maior taxa de crescimento das plantas (912% contra 114% das plantas não-tratadas), além de proporcionar plantas com maior... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Malus prunifolia; Cultura in vitro; Adaptacao; Dormencia; In vitro culture; Adaptation; Dormancy. |
| Ano: 2001 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/103913 |
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| Registros recuperados: 136 | |
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