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Registros recuperados: 6
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Biological and physicochemical properties of cowpea severe mosaic comovirus isolated from soybean in the State of Paraná BABT
Bertacini,Paula V.; Almeida,Álvaro M.R.; Lima,J. Albérsio A.; Chagas,Cesar M..
Soybean plants with symptoms of bud blight were growing close to cowpea with severe symptoms of mosaic associated with blisters in the leaves. A group of plants of both species were collected and used for etiological studies. This kind of symptom in soybeans was common in certain areas of the State of Paraná, induced by tobacco streak ilarvirus. Host range, serological reaction, particle morphology and size, protein and nucleic acid analysis, and transmission by beetles from species Cerotoma arcuata Oliv. showed that the virus involved was cowpea severe mosaic comovirus. This is the first report on the occurrence of this virus in soybean plants in the State of Paraná. Results using indirect ELISA showed that in cowpea the relative virus concentration was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Cowpea severe mosaic comovirus; Indirect ELISA.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400004
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Enzootic bovine Leukosis: development of an indirect enzyme linked immunosorbent assay (I-Elisa) in seroepidemiological studies Rev. Microbiol.
González,Ester Teresa; Bonzo,Estela Beatriz; Echeverría,María Gabriela; Licursi,María; Etcheverrigaray,María Elisa.
Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine Leukaemia; Indirect ELISA; Seroepidemiology.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100007
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Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B. Repositório Alice
RADAELLI, P.; FAJARDO, T.V.M.; NICKEL, O.; EIRAS, M.; PIO-RIBEIRO, G..
The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Vitis; GLRaV-2; GVB; Indirect ELISA; Recombinant protein; Western blot; ELISA indireto; Proteína recombinante; Western blot.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122230
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Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B PAB
Radaelli,Paula; Fajardo,Thor Vinícius Martins; Nickel,Osmar; Eiras,Marcelo; Pio-Ribeiro,Gilvan.
The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis; GLRaV-2; GVB; Indirect ELISA; Recombinant protein; Western blot.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000020
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Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis BJM
Mirhosseini,Seyed Ali; Fooladi,Abbas Ali Imani; Amani,Jafar; Sedighian,Hamid.
ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis; Flagellin C; Indirect ELISA; Bacterial detection; Food contaminated.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400774
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Técnica de microfiltragem seriada para purificação da proteína p28 do vírus da Artrite Encefalite Caprina. Infoteca-e
ANDRIOLI, A.; ALVES, L. A. de O.; TEIXEIRA, M. F. da S.; PINHEIRO, R. R.; DIAS, R. P..
Neste estudo, objetivou-se purificar a proteína antigênica p28 do LVC, pela técnica de microfiltração seriada em membranas AMICON, substituindo a ultracentrifugação em colchão de sacarose (UCCS), para a obtenção de um antígeno a ser empregado em teste ELISA indireto (ELISA-i)
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Artrite-Encefalite Caprina; CAE; Teste ELISA; Indirect ELISA; Caprino; Doença Animal; Diagnostico; Antígeno; Goats; Goat diseases; Animal diseases; Caprine arthritis-encephalitis; Lentivirus; Viral antigens; Diagnostic techniques.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112732
Registros recuperados: 6
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