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Registros recuperados: 12
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A metallic impregnation technique adapted to study the honeybee Apis mellifera L. brain Neotropical Entomology
Calábria,Luciana K; Teixeira,Renata R; Moraes,Viviane R A; Santos,Ana Alice D; Espindola,Foued S.
In order to visualize the distribution pattern of the neuronal bodies and neurofibrils in the honeybee brain, we adapted a metallic impregnation technique first described for vertebrate nervous system by Ramón y Cajal. The honeybee brain constitution plays a key role in the development of learning and memory capacities. The general characteristics observed in the honeybee brain, stained by metallic impregnation, revealed its anatomical and morphological constitution in agreement with studies of other insect brains using different techniques. Metallic impregnation evidenced the optic lobe neuropils, the ocelli fiber cells, the neuron extensions of the calyces, and the axon bundles that involve the antennal glomeruli, as well as the neuron extensions in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurofibril; Neuron; Invertebrate; Silver.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000500008
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Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. ArchiMer
Poirier, Aurore C.; Schmitt, Paulina; Rosa, Rafael D.; Vanhove, Audrey S.; Kieffer-jaquinod, Sylvie; Rubio, Tristan P.; Charriere, Guillaume M.; Destoumieux-garzon, Delphine.
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial...
Tipo: Text Palavras-chave: Antimicrobial Peptide (AMP); DNA; Innate Immunity; Invertebrate; Reactive Oxygen Species (ROS); NET; Mollusk.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00217/32866/31784.pdf
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Cecropins as a marker of Spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge ArchiMer
Duvic, B.; Jouan, V.; Essa, N.; Girard, P-a; Pages, S.; Khattar, Z. Abi; Volkoff, N-a; Givaudan, A.; Destoumieux-garzon, Delphine; Escoubas, Jean-michel.
An antimicrobial peptide (AMP) of the cecropin family was isolated by HPLC from plasma of the insect pest, Spodoptera frugiperda. Its molecular mass is 3910.9 Da as determined by mass spectrometry. Thanks to the EST database Spodobase, we were able to describe 13 cDNAs encoding six different cecropins which belong to the sub-families CecA, CecB, CecC and CecD. The purified peptide identified as CecB1 was chemically synthesized (syCecB1). It was shown to be active against Gram-positive and Gram-negative bacteria as well as fungi. Two closely related entomopathogenic bacteria, Xenorhabdus nematophila F1 and Xenorhabdus mauleonii VC01(T) showed different susceptibility to syCecB1. Indeed, X. nematophila was sensitive to syCecB1 whereas X. mauleonii had a...
Tipo: Text Palavras-chave: Humoral immunity; Innate immunity; Immunosuppression; Lepidopteran; Invertebrate.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00086/19728/17485.pdf
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Contribution à l'étude de l'ontogenèse du système immunitaire chez l'huître creuse Crassostrea gigas ArchiMer
Tirape Bajana, Ana.
The interest for the understanding of the immune mechanisms in the oyster, Crassotrea gigas has been considerably increased these last years, mainly because of economie losses due to diseases which affected the continuity of this species. The studies of these mechanisms contributes to a better understanding of the differential susceptibilities observed in this species against various pathogens. In the present study, our  goal was to define the ontogenesis of immune system in C. gigas during the development, by functional genomic approaches. The studies have been focalized on the analyses of expression and localization of effectors previously identified in adult oysters as implicated in immunity. We have shown, in particular, that hemocytes appear in the...
Tipo: Text Palavras-chave: Invertébré; Mollusque bivalve; Huître; Cra; Immunité; Hémocytes; Hématopoïèse; Développement; Ontogenèse; Expression des gènes; Q-PCR; SSH; Invertebrate; Bivalve Mollusk; Oyster; Crassostrea gigas; Immunity; Hemocytes; Hematopoiesis; Development; Ontogenesis; Gene expression; Q-PCR; SSH.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00440/55113/56565.pdf
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Evidence in oyster of a plasma extracellular superoxide dismutase which binds LPS ArchiMer
Gonzalez, Marcelo; Romestand, Bernard; Fievet, Julie; Huvet, Arnaud; Lebart, M; Gueguen, Yannick; Bachere, Evelyne.
We have characterized in the oyster Crassostrea gigas an extracellular superoxide dismutase (Cg-EcSOD) which appears to bind lipopolysaccharides (LPS). The protein has been purified from the oyster plasma and identified as a Cu/ZnSOD according to its N-terminal sequencing and biological activity. Cg-EcSOD expression and synthesis are restricted to hemocytes as revealed by in situ hybridization and immunocytochemistry. Cg-EcSOD-expressing hemocytes were seen in blood circulation, in connective tissues, and closely associated to endothelium blood vessels. Cg-EcSOD presents in its amino acid sequence a LPS-binding motif found in the endotoxin receptor CD14 and we show that the protein displays an affinity to Escherichia coli bacteria and with LPS and Lipid A....
Tipo: Text Palavras-chave: Beta integrin; Oxidative burst; Hemocyte; Crassostrea gigas; Invertebrate; Mollusc bivalve.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3583.pdf
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Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata ArchiMer
Lassalle, Damien; Tetreau, Guillaume; Pinaud, Silvain; Galinier, Richard; Crickmore, Neil; Gourbal, Benjamain; Duval, David.
Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that...
Tipo: Text Palavras-chave: Pore-forming toxin; Cry toxin; Invertebrate; Biomphalaria glabrata; Host; Pathogen; Vector snail; Innate immunity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71267/69655.pdf
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Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field ArchiMer
De Lorgeril, Julien; Escoubas, Jean Michel; Loubiere, Vincent; Pernet, Fabrice; Le Gall, Patrik; Vergnes, Agnes; Aujoulat, Fabien; Jeannot, Jean-luc; Jumas-bilak, Estelle; Got, Patrice; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne.
Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European farming areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus clade. Ruling out the complexity of the disease, most of our current knowledge has been acquired in controlled experiments. Among the many unsolved questions, it is still ignored what role immunity plays in the capacity oysters have to survive an infectious episode. Here we show that juvenile oysters susceptible to the disease mount an inefficient immune response associated with microbial permissiveness and death. We...
Tipo: Text Palavras-chave: Host pathogen interaction; Innate immunity; Invertebrate; Mollusk; In situ mortality; Total bacteria; Crassostrea gigas.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00433/54470/55845.pdf
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Insulin or insulin-like studies on unicellular organisms: a review BABT
Souza,Alzira Martins Ferreira de; López,Jorge A..
This paper presents a review of studies about insulin and insulin-like substances in prokaryotes, eukaryotes and fungi have been published over the last two decades, constituting an updating of our previous review (1988) which included references to invertebrate insulin or insulin-like substances both in uni- and pluricellular Monera, Protoctista, Fungii and Animal species. This present article reviews experiments and evidence obtained using modern techniques in the understanding of molecule evolution and behaviour, which confirm its very ancient molecular structure origin. The involvement of insulin-like and related material in signalling biological pathway and modulator effects is also reported.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Unicellular; Invertebrate; Insulin; Insulin-like; Insulin receptor.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600017
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Nanoencapsulated Melaleuca alternifolia essential oil exerts anesthetic effects in the brachyuran crab using Neohelice granulate Anais da ABC (AABC)
SOUZA,CARINE F.; LIMA,TÁBATA; BALDISSERA,MATHEUS D.; GEIHS,MÁRCIO A.; MACIEL,FÁBIO E.; NERY,LUIZ E.M.; SANTOS,ROBERTO C.V.; RAFFIN,RENATA P.; HEINZMANN,BERTA M.; CARON,BRAULIO O.; BALDISSEROTTO,BERNARDO.
ABSTRACT The aim of this study was to evaluate the efficacy and safety of several anesthetics in the brachyuran crab Neohelice granulata, an emergent experimental model. The essential oils (EOs) of Lippia alba, Aloysia tryphilla, and Melaleuca alternifolia (tea tree oil; TTO), the isolated compounds eugenol, menthol, terpinen-4-ol, and the nanoencapsulated form of TTO, were administered in one or more of the following ways: added to the water (immersion), through an arthrodial membrane (injected), or by oral gavage. Unexpectedly, most EOs did not produce an anesthetic effect after immersion. Only TTO and eugenol induced anesthesia by immersion, with very long induction and recovery times compared to anesthesia of other crustaceans. However, a good...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Eugenol; Terpinen-4-ol; Nanotechnology; Invertebrate; Tea-tree-oil.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602855
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Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin ArchiMer
Gueguen, Yannick; Romestand, Bernard; Fievet, Julie; Schmitt, Paulina; Destoumieux Garzon, Delphine; Franck, Vandenbulcke; Philippe, Bulet; Bachere, Evelyne.
A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an...
Tipo: Text Palavras-chave: Antimicrobial peptide; Mollusk; Synergy; Invertebrate; Innate immunity; Pacific oyster.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6232.pdf
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Poissons et invertébrés au large des côtes de France : Indicateurs issus des pêches scientifiques. Bilan 2002 ArchiMer
Bertrand, Jacques; Cochard, Marie-laure; Coppin, Franck; Le Pape, Olivier; Mahe, Jean-claude; Morin, Jocelyne; Poulard, Jean-charles; Rochet, Marie-joelle; Schlaich, Ivan; Souplet, Arnauld; Trenkel, Verena; Verin, Yves.
The scientist community, the fishing organisations and all involved parties show a growing interest in an integrated project management of the sea space exploited by fishing communities. A key to reach such a target is to make available reliable and precise scientist data on the different components of the fishing systems. For about twenty years, and in its mission to support the development of an integrated management for a sustainable use of marine resources, Ifremer has been organising monitoring campaigns of fishery resources along the shores of France. These campaigns aim at providing abundance indicator on species of interest. They consequently provide an essential knowledge base to characterize and trace the status of exploited fish and...
Tipo: Text Palavras-chave: Ecosystem; Indicating; Oceanographical campaign; Scientific research; Fishing halieutic; Fishing; English Channel; Bay of Biscay; France; Invertebrate; Pisces; Ecosystème; Indicateur; Campagne océanographique; Recherche scientifique; Pêche halieutique; Pêche; Mer du nord; Golfe de gascogne; Manche; France; Invertebrate; Pisces.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/rapport-1091.pdf
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Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus ArchiMer
Duperthuy, Marylise; Schmitt, Paulina; Garzon, Edwin; Caro, Audrey; Rosa, Rafael D.; Le Roux, Frederique; Lautredou-audouy, Nicole; Got, Patrice; Romestand, Bernard; De Lorgeril, Julien; Kieffer-jaquinod, Sylvie; Bachere, Evelyne; Destoumieux-garzon, Delphine.
OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for beta-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase Cg-EcSOD, is used as an opsonin mediating the...
Tipo: Text Palavras-chave: Host-pathogen interaction; Innate immunity; Invertebrate; Mollusk; Oxidative burst.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14177/12166.pdf
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