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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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A broad pH range and processive chitinase from a metagenome library BJMBR
Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O..
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602
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Adsorption of p-cresol on Granular Activated Carbon CIGR Journal
Lalitendu Das; North Carolina State University Biological and Agricultural Engineering; Praveen Kolar; North Carolina State University; Jason Osborne; North Carolina State University Department of Statistics; John Classen; North Carolina State University Biological and Agricultural Engineering.
Swine farming emit several odorous volatile organic compounds, one of which is p-cresol. Considering the layout of a swine barn, adsorption is one of the most suitable technologies for mitigating organic pollutants. In this study, commercial granular activated carbon (GAC) was tested as an adsorbent for removing p-cresol from aqueous solution. The objectives were to: 1) determine the combined effect of temperature, pH, and adsorbent dose on adsorption, (2) investigate the effect of volatile fatty acids and aldehydes on adsorption of p-cresol, (3) determine adsorption kinetics and isotherms, (4) study the effect of solvent on adsorption, (5) propose a possible mechanism of adsorption, and (6) discuss practical implications and design calculations for...
Palavras-chave: Agricultural Engineering Adsorption; P-cresol; Activated carbon; Kinetics; Isotherms; RSM.
Ano: 2012 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2171
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Antioxidant activity of the extracts from Pycnoporus sanguineus mycelium BABT
Borderes,Josiane; Costa,Alessandra; Guedes,Alessandro; Tavares,Lorena Benathar Ballod.
The aim of this work was to study the production of compounds with antioxidant activity by Pycnoporus sanguineus when cultivated in submerged fermentation using a potato dextrose broth plus peptone medium. The study evaluated the biomass production, glucose consumption, variation of medium pH and antioxidant activity. The antioxidant potential was tested through the DPPH method and β-carotene / linoleic acid system with extracts obtained from the mycelium at different times of cultivation (5, 10, 15, 20, 25 and 30 days). Maximum kinetic values of specific growth rate (0.289 day-1), biomass productivity (0.698 g.L-1.day-1) and the yield of glucose conversion into biomass (26.24 g.g-1) were obtained during the exponential growth phase. The highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycetes; Submerged culture; Kinetics; Antioxidant compounds.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600012
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Biosorption kinetics of a direct azo dye Sirius Blue K-CFN by Trametes versicolor Electron. J. Biotechnol.
Erden,Emre; Kaymaz,Yasin; Pazarlioglu,Nurdan Kasikara.
In this study, lyophilized Trametes versicolor biomass is used as a sorbent for biosorption of a textile dye, Sirius Blue K-CFN, from an aqueous solution. The batch sorption was studied with respect to dye concentration, adsorbent dose and equilibrium time. The effect of pH and temperature on dye uptake was also investigated and kinetic parameters were determined. Optimal initial pH (3.0), equilibrium time (2 hrs), initial dye concentration ( 100 mg l-1) and biomass concentration (1.2 mg l-1) were determined at 26�C. The maximum biosorption capacity (q max) of Sirius Blue K-CFN dye on lyophilized T. versicolor biomass is 62.62 mg/g. The kinetic and isotherm studies indicated that the biosorption process obeys to a pseudo-second order model and Langmuir...
Tipo: Journal article Palavras-chave: Biosorbent; Biosorption; Direct dye; Kinetics; Sirius Blue K-CFN; Trametes versicolor.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200003
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Biosorption of lead(II) and chromium(VI) on groundnut hull: Equilibrium, kinetics and thermodynamics study Electron. J. Biotechnol.
Qaiser,Suleman; Saleemi,Anwar R; Umar,Muhammad.
The biosorption of lead(II) and chromium(VI) on groundnut hull was investigated. Batch biosorption experiments were conducted to find the equilibrium time and biosorption capacity. Effect of parameters like pH, temperature and initial metal concentration was studied. The maximum biosorption capacity of lead(II) and chromium(VI) was found to be 31.54 ± 0.63 and 30.21 ± 0.74 mg g-1, respectively. The optimum pH for lead(II) and chromium(VI) removal was 5 ± 0.1 and 2 ± 0.1, respectively. The temperature change, in the range of 20 - 45ºC affected the biosorption capacity. The maximum removal of lead(II) was achieved at 20 ± 2ºC, where as maximum uptake of chromium(VI) was observed at 40 ± 2ºC. The biosorption data was fitted to the Langmuir and the Freundlich...
Tipo: Journal article Palavras-chave: Biosorption; Equilibrium; Groundnut hull; Kinetics; Thermodynamics.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400003
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Biotransformation of anthracene and fluoranthene by Absidia fusca Linnemann Electron. J. Biotechnol.
Villemain,Danièle; Guiraud,Pascale; Bordjiba,Ouahiba; Steiman,Régine.
A strain of Absidia fusca was isolated from a pesticide-contaminated soil (Annaba, Algeria). The biotransformation capability of this strain towards two polycyclic aromatic hydrocarbons (PAHs): anthracene and fluoranthene was compared to that exhibited by another strain of A. fusca isolated from a non-contaminated milieu and considered as a control. The results obtained were statistically analyzed and showed that the strain isolated from the contaminated soil was more efficient than the control to remove anthracene from the medium, during all the kinetics (90% removed versus 45% after 24 hrs). Concerning fluoranthene, the amount removed by both strains was very high during the first 24 hrs however the control strain was slightly more efficient (94% versus...
Tipo: Journal article Palavras-chave: Absidia fusca; Anthracene; Biodegradation; Fluoranthene; Kinetics.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200004
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Cellulase effect on anaerobic digestion of maize silage under discontinuous operation REA
Oliva-Merencio,Deny; Pereda-Reyes,Ileana; Schimpf,Ulrike; Koehler,Stefan; Silva,Ariovaldo J. da.
ABSTRACT This paper studied the effect of adding an enzyme (ellulose) on anaerobic digestion of maize silage. We compared materials at chopping lengths of 8 mm (MSL), 4mm (MSS) and natural size (Ms) under a mesophilic and discontinuous operation (batch process). Hence, we found the process to be significantly influenced by particle size. Moreover, the ellulose addition did not significantly impact biogas production after a 35-day digestion period. Ms and MSS displayed an improved response to all variables when compared with MSL and MSL+C, with significant differences. Studies on the refractory fraction at infinite time (R0) have demonstrated that the lowest values correspond to Ms and MSS (0.122 and 0.155, respectively). The Kinetic approach and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ellulose; Silage; Biogas; Kinetics; Biodegradability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162015000500951
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Chemical kinetics of aerobic soils and rice growth International Rice Research Institute
Kundu, Dilip Kumar.
346 l. : ill. Thesis (Ph.D.) -- Indian Agricultural Research Institute
Tipo: Thesis Palavras-chave: Rice; Soil; Soil chemistry; Kinetics; Aerobic conditions; Soil-plant interactions; Plant growth; Plant nutrition.
Ano: 1987 URL: http://hdl.handle.net/123456789/1311
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Cinética de sorção de fósforo em alguns solos de Minas Gerais. Repositório Alice
REIS, C.; FABRIS, J.D.; NOVAIS, R.F.; BAHIA FILHO, A.F.C.; SANTANA, D. P.; CURI, N.; COEY, J.M.D..
Os processos cineticos e termodinamicos envolvidos nos mecanismos de troca de fosforo no sistema fase solida-solucao do solo sao ainda pouco conhecidos. O proposito do presente trabalho foi investigar o comportamento dinamico do fosforo em sistemas multifasicos. Como fase solida, foram utilizadas as fracoes argila de onze amostras de duas pedossequencias do Estado de Minas Gerais: uma com litologia variando em proporcoes de rochas peliticas e de tufito; outra de arenito e de basalto. E proposto um modelo cinetico para descrever a dinamica de sorcao do fosforo. Estimaram-se as constantes de taxa de reacao (ki, i = + ou - 1, + ou - 2) de primeira ordem, para as duas primeiras etapas consecutivas do mecanismo multiplo de reacao. Na maior parte das amostras,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cinetica; Sorcao; Solos magneticos; Oxidos de ferro; Fosforo; Minas Gerais; Soils; Kinetics; Phosphorus; Sorption.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/471663
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Co-crystallization of paprika oleoresin and storage stability study Ciênc. Tecnol. Aliment.
FEDERZONI,Veronica; ALVIM,Izabela Dutra; FADINI,Ana Lúcia; SILVA,Lidiane Bataglia da; QUEIROZ,Marise Bonifácio.
Abstract Paprika oleoresin was co-crystallized to formulate a natural and hydrophilic dye for use in food applications. The color stabilities of the co-crystallized and free oleoresins were evaluated at different temperatures (25 and 35 °C, 70% RH) and light exposures (light 70 watts and dark, 25 °C, 70% RH). Co-crystallization from supersaturated sucrose syrup (S = 1.32) was found to be optimum for the formation of sugar agglomerates in a few minutes, with the paprika oleoresin entrapped inside. With time, decreases in the values ​​of the a* parameter and the β-carotene content were observed with increasing temperature and with the incidence of light. The color degradation kinetics were dependent on both factors. The loss of color was greater for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-crystallization; Paprika oleoresin; Β-carotene; Stability; Kinetics; Color.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000500182
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Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum Electron. J. Biotechnol.
Ahmed,Sibtain; Imdad,Syeda Sana; Jamil,Amer.
Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T....
Tipo: Journal article Palavras-chave: C. thermophilum; Kinetics; T. harzianum; Xylanase.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003
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Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor BJM
Mukhtar,Hamid; Ikram-ul-Haq,.
We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The optimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simultaneous; Sporulation; Kinetics; Fermentation; Agitation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033
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Continuous fluorescence recording as a way to improve Pacific oyster (Crassostrea gigas) models of paralytic shellfish toxin accumulation ArchiMer
Baron, Regis; Couedel, Marielle; Joret, Camille; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Lassus, Patrick.
A simple system was used to simulate the effect of alternating toxic (paralytic shellfish poisoning toxins) and non-toxic microalgal diets on oyster feeding behaviors and rates of toxin accumulation. These experimental conditions were meant to reflect, to some extent, the incoming and outgoing fluxes of toxic algae observed at the mouth of the Penze estuary (Northern Brittany, France). Physiological and toxicological parameters were estimated based oil fluorescence measurements recorded continuously at the outlet of each experimental tank, which contained a single oyster. Q(tox), this variable describes toxin uptake in oysters, it was used (instead of the toxin ingestion rate): i) in simple graphical analyses, ii) as well as in one- and two-compartment...
Tipo: Text Palavras-chave: Skeletonema costatum; Alexandrium minutum; Fluorescence; Modeling; Kinetics; Toxin uptake; Shellfish toxin; PSP.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1400.pdf
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Determination of drying characteristics and kinetics of bitter kola (garcinia kola) using page’s model CIGR Journal
Chinaka, Ehiem James.
Single-layer drying characteristics and kinetics of garcinia kola was studied with slice thicknesses of 1mm, 3mm, 5mm and 7mm at temperatures 45oC, 55oC, 65oC and 75oC using natural convention laboratory oven . The results obtained revealed that garcinia kola slices dried in the falling rate period. Moisture ratio, characteristics drying rate constant, drying time, effective diffusivity and activation energy were significantly (p
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural & Bioresources Engineering/Postharvest Processing and Storage Engineering Garcinia kola; Kinetics; Diffusion; Activation energy; Page’s model.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2987
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes Arq. Bras. Med. Vet. Zootec.
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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Effects of pyrogenic carbon feedstock and pyrolysis temperature on the oxidation kinetic and benzene polycarboxilic acids formation. Repositório Alice
CERQUEIRA, W. V.; RITTL, T. F.; PINHEIRO, M. A.; PEREIRA NETTO, A. D.; NOVOTNY, E. H..
Pyrogenic carbon (PyC) has a high potential to soil carbon sequestration. Among the methods used to quantify PyC, the method of benzene polycarboxilic acids (BPCA) is well-established. The oxidation step of this method is essential for a reliable PyC quantification. Up to now there are no studies on the influence of feedstock and pyrolysis temperature in the oxidation kinetic of PyC and BPCA formation; and then these are the purposes of this study. For this, different PyC were oxidised at 170 ºC during different times. The data showed similar kinetic curves for all PyC, but different BPCA production depending upon feedstock and pyrolysis temperature. All PyC showed maximum production of BPCA at 6-8 h under nitric acid oxidation at 170 ºC. We concluded that...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Cinética de oxidação; Biochar; Ácido benzenopolicarboxílico; Quantificação de carbono pirogênico; Ácido; Carbono; Oxidação; Kinetics; Oxidation; Carbon; Acids.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1001606
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Estimation of soil nitrogen supply in lowland rice soils based on mineralization kinetics under anaerobic incubation International Rice Research Institute
Samson, Marianne I..
xv, 86 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños,
Tipo: Thesis Palavras-chave: Rice soils; Soil nutrient balance; Nitrogen; Mineralization; Lowlands; Kinetics; Incubation.
Ano: 1997 URL: http://hdl.handle.net/123456789/571
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Exploring methane-hydrate formation and dissociation in geologic materials through laboratory experiments: Kinetic behavior and morphology ArchiMer
Ruffine, Livio.
To gain in-depth understanding of natural gas hydrate behavior it is necessary to identify key parameters that affect their formation, distribution and destabilization within sediments. Hydrate formation kinetics in porous media is amongst the aspects which deserve important considerations as it may provide useful information on the formation history and the formation mechanisms of natural gas hydrate accumulations. Yet, it is at its early stage. In this paper, experiments on methane hydrate formation and dissociation in porous media are reported and discussed. The first part of this work is devoted to the investigation of the kinetics of methane hydrate formation within silica sand using a custom-design apparatus. The latter is suitable for investigating...
Tipo: Text Palavras-chave: Geologic matrices; Hydrate morphology; Kinetics; Methane hydrates; Novel apparatus.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00245/35580/34209.pdf
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Extended storage of cold raw milk on yogurt manufacturing PAB
Fagnani,Rafael; Schuck,Josiane; Botaro,Bruno Garcia; Santos,Fernando Cesar dos.
Abstract: The objective of this work was to evaluate the effect of the extended cold storage of raw milk on the kinetics of fermentation on yogurt production, as well as on the product’s microbiological and physicochemical properties during shelf life. Three treatments were evaluated: yogurts made with raw milk stored for 4, 72, and 168 hours. Kinetics of fermentation was assessed through the lactic acid production rate, growth rate of Streptococcus thermophilus and Lactobacillus bulgaricus, and time to reach pH 5.0 and 4.5. The physicochemical and microbiological characteristics of raw milk and yogurts were also analyzed during cold storage at 4°C. The microbial quality of raw milk was affected by the storage time of 168 hours, with a decreasing tendency...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus bulgaricus; Streptococcus thermophilus; Fermentation; Kinetics; Psychrotrophs..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000200104
Registros recuperados: 56
Primeira ... 123 ... Última
 

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