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Registros recuperados: 36
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An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Repositório Alice
PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de.
Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triagem; Exopolissacarídeos; Análise de laboratório; Polissacarídeo; Screening; Exopolysaccharides; Lactic acid bacteria.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/963060
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Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products BJM
Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z..
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008
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Antimicrobial compounds produced by Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product. Repositório Alice
CARVALHO, K. G. de; BAMBIRRA, F. H. S.; KRUGER, M. F; BARBOSA, M. S.; OLIVEIRA, J. S.; SANTOS, A. M. C.; NICOLI, J. R.; BEMQUERER, M. P.; MIRANDA, A. de; SALVUCCI, E. J.; SESMA, F. J. M.; FRANCO, B. D. G. M..
bitstream/item/181795/1/Carvalho2010-Article-AntimicrobialCompoundsProduced.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Lactobacillus sakei 2a; Antimicrobial proteins; Bacteriocins; Lactic acid bacteria.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/869794
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Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products ArchiMer
Pilet, Marie-france; Leroi, Francoise.
Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...
Tipo: Text Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf
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Atividade proteolítica de cepas de Lactobacillus mucosae isoladas de leite de cabra. Repositório Alice
MORAES, G. D. de; DUARTE, T. F.; SILVA, L. M. F. da; FIGUEIREDO, H. O. S.; VASCONCELOS, A. S. do E.; SANTOS, K. M. O. dos..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Lactobacillus mucosae; Probiótico; Produto lácteo; Caprino; Leite de cabra; Proteólise; Tecnologia de alimento; Goat milk; Probiotics; Lactobacillus; Lactic acid bacteria; Food technology.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/975127
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Avaliação do potencial probiótico e tecnológico de bactérias ácido láticas isoladas de queijo de coalho Repositório Alice
LIMA, C. P. de.
Probióticos são micro-organismos vivos que conferem benefícios à saúde do hospedeiro quando administrados em quantidades adequadas e regularmente. Um dos seus principais benefícios está associado a sua participação na modulação da flora intestinal. Produtos lácteos são reconhecidos como uma excelente fonte e veículo de bactérias láticas probióticas. O objetivo deste estudo foi caracterizar o potencial probiótico e tecnológico de 24 cepas de bactérias ácido láticas (BAL) oriundas de queijo de Coalho artesanal produzidos em Pernambuco. Foram realizados testes de resistência ao trato gastrintestinal (TGI), atividade antimicrobiana frente a patógenos intestinais, capacidade de autoagregação e coagregação, hidrofobicidade celular, atividade ß-galactosidase,...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Queijo coalho; Probiotics; Lactic acid bacteria; Functional foods.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101893
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Caracterización de cultivos iniciadores en productos cárnicos. Parte 1 OceanDocs
Beldarraín, T.; Cepero, Y.; Bruselas, A.; Santos, R.; Ramos, M.; Moya, Y.; Núñez, M.; Vergara, N..
En este trabajo se caracterizaron tecnológicamente, a escala de laboratorio, 5 cultivos iniciadores. Las propiedades tecnológicas evaluadas fueron pH en caldo glucosado, viabilidad del cultivo, tolerancia a elevada concentración de sal, inactivación a 60 ºC por 30 min, entre otras. Todos los cultivos, por sus aptitudes acidificantes y población celular se consideraron aptos para su empleo industrial y se pudieron categorizar como productores de baja y alta acidez.
Tipo: Journal Contribution Palavras-chave: Bacteria; Starters; Lactic acid bacteria; Microbial starters; Media production; Meat products.
Ano: 2008 URL: http://hdl.handle.net/1834/4896
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Characterization of bacteriocin production, safety and technological potential of two Enterococcus faecium stains isolated from Brazilian Artisanal Cheeses. Repositório Alice
SANTOS, K. M. O. dos; VIEIRA, A. D. S.; OLIVEIRA, J. da; ROCHA, C. R. C.; LOPES, A. C. S.; BRUNO, L. M.; BORGES, M. de F.; FRANCO, B. D. G. M.; TODOROV, S..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Produção artesanal; Queijo artesanal; Anti-Listeriamonocytogenes activity; Bacteriocin-like substance; Bactérias láticas; Tecnologia de alimento; Coalho; Cheese; Bacteriocin; Enterococcus faecalis; Enterococcus faecium; Lactic acid bacteria.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/977456
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Efecto de los niveles de Vitafert y melaza en la pollinaza fermentada aeróbica. Colegio de Postgraduados
Arias López, Flor de Tila.
La pollinaza es la excreta de las aves de engorda y se ha utilizado en la suplementación de los bovinos debido al elevado contenido de nitrógeno no proteínico (NNP) y por su bajo costo. Con el objetivo de mejorar los parámetros bromatológicos y fermentativos de la pollinaza. Se estudió en diseño completamente al azar con arreglo factorial 3 x 3 con 3 repeticiones por tratamientos, con variable de respuesta incremento en Y, el efecto de los niveles de Vitafert (0, 30 y 60%) y melaza (10, 20, y 30%) Se mezclo la pollinaza con los porcentajes de Vitafert y melaza de forma homogénea, se utilizo 1kg de muestra como unidad experimental agregando agua a los tratamientos para mantener una proporción del 60 % de humedad, una vez mezclados los ingredientes...
Palavras-chave: Pollinaza; Vitafert; Melaza; Proteína cruda; Proteína verdadera; PH; Bacteria ácido láctica; Ooquiste; Manure; Molasses; Protein raw; True protein; Lactic acid bacteria; Oocyst; Maestría; Producción Agroalimentaria en el Trópico.
Ano: 2010 URL: http://hdl.handle.net/10521/287
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Effects of carbohydrate source on physicochemical properties of the exopolysaccharide produced by Lactobacillus fermentum TDS030603 in a chemically defined medium OAK
Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly...
Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694
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Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites Electron. J. Biotechnol.
Serna-Cock,Liliana; Enríquez-Valencia,Cruz E; Jiménez-Obando,Eliana M; Campos-Gaona,Rómulo.
Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were...
Tipo: Journal article Palavras-chave: Conservation; Inhibition; Lactic acid bacteria; Lyophilization.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300006
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Genes encoding for intestinal mucus adhesion factors in lactobacillus mucosae strains isolated from brazilian goat milk. Repositório Alice
MORAES, G. M. D. de; ABREU, L. R. de; EGITO, A. S. do; TODOROV, S.; SANTOS, K. M. O. dos.
Molecular biology can be outstanding tool in the selection of probiotic candidates, based on the detection of genetic factors related to the beneficial properties of studied lactic acid bacteria (LAB). Surface proteins with mucus-binding capacity mediate the adherence of LAB to the intestine and are desirable feature for probiotic bacteria, as they can increase persistence and ability of LAB to colonize the intestine. In order to investigate mucus adhesion properties, the presence of genes encoding for adhesion proteins were investigated in three Lactobacillus mucosae strains isolated from goat milk.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Gastro-intestinal tract; Lactic acid bacteria; Probiotic potential.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1057814
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High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses Electron. J. Biotechnol.
Krzywonos,Magorzata; Eberhard,Thomas.
Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10%...
Tipo: Journal article Palavras-chave: Distillery wastewater; High density fermentation; Lactic acid bacteria; Lactobacillus plantarum; Sugar beet molasses; Wheat stillage.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200006
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Identificação e caracterização do potencial probiótico de bactérias isoladas do leite e queijo caprino. Repositório Alice
ABREU, L. R. DE.
Abstract: A busca por novas bactérias com propriedades funcionais tem sido foco de intensa pesquisa nas últimas décadas. Diversas espécies do gênero Lactobacillus já são conhecidas como probióticas e são adicionadas em alimentos. Para uma cultura bactériana ser selecionada como probiótica é preciso apresentar determinadas características, como a capacidade de sobreviver às condições gastrointestinais e ser livre de patogenicidade. O objetivo desse estudo foi isolar, selecionar e identificar cepas de lactobacilos a partir de leite e queijo de cabra, e avaliar seu potencial probiótico e sua inocuidade através da identificação de genes relacionados às características benéficas e de riscos para o consumo humano, verificando sua expressão in vitro. Foram...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Probiótico; Alimentos funcionais; Bactérias ácido láticas; In vitro expression; Caprino; Virulência; Leite de cabra; Queijo; Produto derivado do leite; Tecnologia de alimento; Probiotics; Genes; Goat milk; Lactobacillus; Lactic acid bacteria; Food technolog.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074920
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Influence of temperature, pH and NaCl concentration on the maximal growth rate of Brochothrix thermosphacta and a bioprotective bacteria Lactococcus piscium CNCM I-4031 ArchiMer
Leroi, Francoise; Fall, Papa-abdoulaye; Pilet, Marie-france; Chevalier, Frederique; Baron, Regis.
The maximum specific growth rate (μmax) of Brochothrix thermosphacta, a spoilage bacteria of cooked peeled shrimp, and Lactococcus piscium CNCM I-4031, a bioprotective strain, was investigated under different conditions of temperature, NaCl concentrations and pH. The basic modelling approach used was the Gamma concept (γ-concept) and the model developed was then adapted to shrimp. Cardinal growth parameters were quite similar for the two strains, except for NaCl. No NaCl was required for growth and the NaClmax was three-times higher for B. thermosphacta than for L. piscium (62 and 23 g l−1 respectively). However, tolerance to NaCl was higher in seafood than in liquid broth, possibly due to presence of osmoltically active molecules. L. piscium and B....
Tipo: Text Palavras-chave: Modelling; Biopreservation; Shrimp; Lactic acid bacteria; Brochothrix thermosphacta; Spoilage bacteria; Environmental conditions.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19473/17088.pdf
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Interaction of Saccharomyces cerevisiae and Lactococcus lactis in the fermentation and quality of artisanal cachaça Agronomy
Carvalho, Fernanda Paula; Duarte, Whasley Ferreira; Dias, Disney Ribeiro; Piccoli, Roberta Hilsdorf; Schwan, Rosane Freitas.
Lactococcus lactis and Saccharomyces cerevisiae in co-culture were evaluated during sugar cane fermentantion for cachaça production. The inocula containing L. lactis UFLA CA 312 and S. cerevisiae UFLA CA 11 were used in the population of approximately 105 CFU mL-1 and 108 CFU mL-1,  respectively. The sugar cane medium plus 1% of yeast extract (SCM) was efficient for growth of L. lactis UFLA CA 312 and S. cerevisiae UFLA CA 11 (letter b -Tukey test). In flasks and vats fermentation the growth of UFLA CA 11 was not negatively influenced by L. lactis UFLA CA 312. However, after 19 h of fermentation, bacterial population showed a slight decrease. Considering parameters higher alcohols and aldehydes, cachaça produced by pure culture of S. cerevisiae was similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.02.04-4 microbiologia fermentacao alcoolica cachaça leveduras bacterias Microbiologia Agricola sugar cane spirit; Mixed culture; Lactic acid bacteria; Starter culture.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18397
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Lactobacillus fermentum TDS030603株の生産する中性多糖の構造研究 OAK
Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6...
Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183
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Lactococcus piscium : a psychotrophic lactic acid bacterium with bioprotective or spoilage activity in food - a review ArchiMer
Saraoui, Taous; Leroi, Francoise; Bjorkroth, J.; Pilet, M. F..
The genus Lactococcus comprises twelve species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium (LAB), probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species has been isolated from a large variety of food: meat, seafood and vegetables, mostly packed under vacuum (VP) or modified atmosphere (MAP) and stored at chilled temperature. Recently, culture-independent techniques used for characterization of microbial ecosystems have highlighted the importance of L. piscium in food. Its role in food spoilage varies according to the strain and the food matrix. However, most studies have indicated...
Tipo: Text Palavras-chave: Biopreservation; Cold adaptation; Lactic acid bacteria; Meat; Seafood; Spoilage.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00334/44517/44213.pdf
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Maîtrise du développement de Listeria monocytogenes dans le saumon fumé : intérêt de la biopréservation par des bactéries lactiques ArchiMer
Richard, Christelle; Leroi, Francoise; Brillet-viel, Anne; Rachman, Cinta; Connil, Nathalie; Drider, Djamel; Pilet, Marie-france; Onno, Bernard; Dousset, Xavier; Prevost, Hervé.
The interest of biopreservation using lactic bacteria to improve the food security of smoked salmon was evaluated. Cold smoked salmon is a non-stable microbiological product whose physicochemical characteristics allow the development of Listeria monocytogenes which constitutes the major microbiological risk depending on the consumption of this product. Biodiversity of the microflora of smoked salmon and its evolution during conservation, the origin of the contamination by Listeria and its consequences, and the parameters of the manufacturing process allowing a better control of microbiological quality are discussed. The innovating concept of biopreservation of smoked salmon is developed. It consists of an inoculation of salmon by a competitive lactic flora...
Tipo: Text Palavras-chave: Saumon fumé; Bactérie lactique; Biopréservation; Listeria; Carnobacterium; Cold smoked salmon; Lactic acid bacteria; Biopreservation; Listeria; Carnobacterium.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10768/7509.pdf
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Occurrence and role of lactic acid bacteria in seafood products ArchiMer
Leroi, Francoise.
Lactic acid bacteria (LAB) in fish flesh has long been disregarded because the high post-mortem pH, the low percentage of sugars, the high content of low molecular weight nitrogenous molecules and the low temperature of temperate waters favor the rapid growth of pH-sensitive psychrotolerant marine Gram-negative bacteria like Pseudomonas, Shewanella and Photobacterium. In seafood packed in both vacuum (VP) and modified atmosphere (MAP) packaging commonly CO2 enriched, the growth of the Gram-negative aerobic bacteria group (predominantly pseudomonads) is effectively inhibited and the number reached by LAB during storage is higher than that achieved in air but always several log units lower than the trimethylamine oxide (TMA-O) reducing and CO2-resistant...
Tipo: Text Palavras-chave: Lactic acid bacteria; Fish; Spoilage; Biopreservation; Probiotic; Fermentation.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11750/8447.pdf
Registros recuperados: 36
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