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Registros recuperados: 71
Primeira ... 1234 ... Última
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Actividad antagonista de bacterias lácticas aisladas del medio marino contra cepas de Listeria ABCL
Marguet,Emilio Rogelio; Vallejo,Marisol; Sierralta Chichisola,Verónica; Quispe,Jorge León.
En el presente trabajo se estudiaron 74 bacterias lácticas para determinar su capacidad de inhibir el desarrollo de Listeria monocytogenes ATCC 7644 o L. innocua ATCC 33090. Dentro de las 7 que exhibieron actividad, 2 se seleccionaron para estudios complementarios y se identificaron por métodos fenotípicos y genotípicos como Enterococcus mundtii Tw 56 y Lactococcus lactis subsp. lactis Tw 34. Después de la neutralización con NaOH y tratamiento térmico (100 ºC y 121 ºC durante 5 min) la actividad antagonista del sobrenadante libre de células se mantuvo activa. El tratamiento con lisozima, lipasa y catalasa no afectó la actividad antimicrobiana. Sin embargo, la actividad enzimática de la tripsina suprimió la acción inhibitoria del sobrenadante, confirmando...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterias lácticas; Listeria monocytogenes; Bacteriocinas; Ambiente marino.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000200008
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Adaptative response of Listeria monocylogenes to osmotic and chill stress: consequences on food safety. ArchiMer
Elmnasser, Noura; Ritz Bricaud, Magali; Guillou, Sandrine; Leroi, Francoise; Orange, Nicole; Bakhrouf, Amira; Federighi, Michel.
Listeria monocytogenes is it psychrotrophic food-borne pathogen. This organism is problematic for food production industry due to its ubiquitous distribution ill nature and its tolerance to condition of osmotic and chill stress. One common strategy employed by many bacteria and by L. monocytogenes for adaptation to osmotic stress is by the intracellular accumulation of compatible solutes. Two different types of adaptative reponses to low temperature have undertaken by L. monocytogenes. One of the responses is accomplished by adjusting membrane fluidity through alteration of the membrane fatty acid composition. A second response consists ill the accumulation of osmolytes. In this brief review we will focus on these general systems Which are Of particular...
Tipo: Text Palavras-chave: Food safety; Chill stres; Osmotic stress; Adaptation; Listeria monocytogenes; Sécurité des aliments; Stress froid; Stress salin; Adaptation; Listeria monocytogenes.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2549.pdf
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Análise microbiológica do queijo de cabra 'tipo coalho' produzido pela Embrapa Caprinos, quanto à presença de Listeria monocytogenes. Repositório Alice
ALVES, F. S. F.; NEVES, M. R. M. das; SOUSA, F. G. C. de; CHAPAVAL, L.; BRITO, J. R. F.; SANTIAGO, L. B..
O leite de cabra e seus derivados, como qualquer outro alimento, são passíveis de contaminação por microorganismos patogênicos, entre eles a Listeria monocytogenes, e podem causar toxi-infecção alimentar nos consumidores, tornando-se um problema de Saúde Pública. O presente trabalho tem como objetivo analisar microbiologicamente o queijo de cabra 'tipo coalho' produzido pela EMBRAPA Caprinos, quanto à presença de L. monocytogenes e verificar a segurança microbiológica do produto, em relação à presença deste microrganismo. 25 g, de cada uma das 117 amostras de queijo analisadas, foram adicionados a 225 mL do meio Listeria Enrichment Broth e incubados a 30°C por 24 h. 100 µL desta solução foram adicionados a 10 mL do meio Fraser Broth Base suplementado e...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Microbiologia de alimentos; Caprino; Produto derivado do leite; Queijo de cabra; Coalho; Contaminação; Qualidade; Listeria monocytogenes.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/533676
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Antibacterial activity of bacteriocin-like substance P34 on Listeria monocytogenes in chicken sausage BJM
Sant'Anna,Voltaire; Quadros,Deoni A.F.; Motta,Amanda S.; Brandelli,Adriano.
The antimicrobial activity of the bacteriocin-like substance (BLS) P34 against Listeria monocytogenes was investigated in chicken sausage. The BLS was applied to chicken sausages (256 AU g-1) previously inoculated with a suspension of 10² cfu g-1 of L. monocytogenes. BLS P34 inhibited the indicator microorganism in situ in all incubation times for up to 10 days at 5 °C. The effectiveness of BLS P34 was increased when it was added in combination with nisin. The bacteriocin was also tested in natural eatable natural bovine wrapping (salty semi-dried tripe) against the same indicator microorganism, also showing inhibitory capability in vitro. BLS P34 showed potential to control L. monocytogenes in refrigerated meat products.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteriocin; Chilled chicken sausage; Listeria monocytogenes; Biopreservation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400019
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Antibacterial efficacy of nisin, bacteriophage P100 and sodium lactate against Listeria monocytogenes in ready-to-eat sliced pork ham BJM
Figueiredo,Ana Cláudia L.; Almeida,Rogeria C.C..
ABSTRACT The effectiveness of bacteriophage P100, nisin and sodium lactate, individually and in combination, in inhibiting Listeria monocytogenes in ready-to-eat pork ham slices was assessed. The antimicrobials were applied to the surfaces of ready-to-eat pork ham slices, which were inoculated with a mixture of L. monocytogenes. Among the individual antimicrobial treatments, bacteriophage P100 was the most effective, decreasing L. monocytogenes to undetectable levels at zero and 72 h post-infection. Sodium lactate was the least effective treatment. Treatment with nisin at zero h significantly reduced initial cell density (p < 0.05). However, this pattern was not observed at 72 h of storage. A significant difference (p < 0.05) existed between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobials; Food safety; Meat products; Listeria monocytogenes.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400724
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Antibacterial efficacy of Nisin, Pediocin 34 and Enterocin FH99 against Listeria monocytogenes and cross resistance of its bacteriocin resistant variants to common food preservatives BJM
Kaur,G.; Singh,T.P.; Malik,R.K..
Antilisterial efficiency of three bacteriocins, viz, Nisin, Pediocin 34 and Enterocin FH99 was tested individually and in combination against Listeria mononcytogenes ATCC 53135. A greater antibacterial effect was observed when the bacteriocins were combined in pairs, indicating that the use of more than one LAB bacteriocin in combination have a higher antibacterial action than when used individually. Variants of Listeria monocytogenes ATCC 53135 resistant to Nisin, Pediocin 34 and Enterocin FH99 were developed. Bacteriocin cross-resistance of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class. Resistance to Pediocin 34 conferred cross...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Nisin; Pedioin 34; Enterocin FH99; Cross resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100009
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Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products BJM
Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z..
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008
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Antimicrobial activity of autoclaved and non autoclaved copaiba oil on Listeria monocytogenes Ciência Rural
Pieri,Fábio Alessandro; José,Raphaela Mansur; Galvão,Newton Nascentes; Nero,Luis Augusto; Moreira,Maria Aparecida Scatamburlo.
The aim of this study was to evaluate the antimicrobial effect of different copaiba oil concentrations against the growth of Listeria monocytogenes, and analyze differences in inhibition of microorganisms with autoclaved and non autoclaved oil. This study provided an agar diffusion test with six isolates of bacteria and six different concentrations of autoclaved or non autoclaved copaiba oil and a negative control. The results showed sensitivity of five L. monocytogenes isolates related to the 10% autoclaved solution of copaiba oil. Four strains also showed sensitivity to the 5% autoclaved solution and one to 2.5% autoclaved solution. The 10% non autoclaved oil solution showed growth inhibition only for two strains. These results had pointed the 10%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytotherapics; Copaifera; Antimicrobial drugs; Listeria monocytogenes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000800020
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Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila BJM
Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G..
Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011
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Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk Arq. Bras. Med. Vet. Zootec.
Perin,L.M.; Miranda,R.O.; Camargo,A.C.; Colombo,M.; Carvalho,A.F.; Nero,L.A..
The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65ºC for 30min, 75ºC for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisin Z; Milk; Antagonistic activity; Lactococcus lactis subsp. lactis; Listeria monocytogenes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500037
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Antimicrobial effect of copper surfaces on bacteria isolated from poultry meat BJM
Parra,Angel; Toro,Magaly; Jacob,Ricardo; Navarrete,Paola; Troncoso,Miriam; Figueroa,Guillermo; Reyes-Jara,Angélica.
Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens – Salmonella enterica and Listeria monocytogenes – and a poultry native microbiota bacterial species – Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial copper-alloys; Foodborne pathogens; Salmonella; Listeria monocytogenes; Poultry meat.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500113
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Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products ArchiMer
Pilet, Marie-france; Leroi, Francoise.
Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...
Tipo: Text Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf
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Atividade antimicrobiana de bactérias lácticas de embutidos curados frente a Listeria monocytogenes Arq. Bras. Med. Vet. Zootec.
Prado,C.S.; Santos,W.L.M.; Carvalho,C.R.; Moreira,E.C.; Costa,O..
Cepas de bactérias lácticas recuperadas de 336 colônias isoladas e selecionadas foram submetidas ao teste de atividade antimicrobiana direta, que identificou as produtoras de substâncias antimicrobianas capazes de inibir in vitro o desenvolvimento de duas cepas indicadoras de Listeria monocytogenes. As 108 cepas que inibiram diretamente pelo menos uma das cepas indicadoras receberam a denominação DTEI e foram selecionadas para o teste de atividade antimicrobiana indireta contra as mesmas cepas de L. monocytogenes, assim como frente a outras cepas de bactérias lácticas de origens diversas. Essa atividade inibidora indireta foi avaliada por meio de sobrenadantes isentos de células, esterilizados por meio de microfiltração, eliminando-se os principais...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bactéria láctica; Atividade antimicrobiana; Listeria monocytogenes; Lactobacillus sp.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400022
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Atividade antimicrobiana de cepas de Streptococcus spp. isoladas de leite de cabra contra Listeria monocytogenes. Repositório Alice
VIEIRA, A. D. S.; TODOROV, S. D.; SAAD, S. M. I.; FRANCO, B. D. G. de M.; SANTOS, K. M. O. dos.
2011
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Leite de cabra; Listeria monocytogenes; Streptococcus spp..
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/903176
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Behaviour of L. monocytogenes in sliced, vacuum-packed mortadella BJM
Bersot,Luciano dos Santos; Gillio,Cíntia; Tavolaro,Paula; Landgraf,Mariza; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa.
This study evaluated the growth of naturally occurring L. monocytogenes in sliced, vacuum-packed mortadella samples during storage at 5ºC until the expiration date. Tukey's test indicated that counts of L. monocytogenes on 0, 10, 20, 30 and 40 days of storage were significantly different (p<0.05), indicating growth during shelf life. In three trials, the mean increase was 1.72 log cycles. Vacuum packing and storage under refrigeration were not effective in controlling the growth of L. monocytogenes in sliced mortadella, indicating that good manufacturing practices and implemented HACCP programs are essential to assure safety of this product.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Growth; Sliced mortadella; Vacuum-packing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300020
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Biocontrole de Listeria monocytogenes por Pediococcus acidilactici em couve minimamente processada Ciênc. Tecnol. Aliment.
Costa,Wanessa Altimiras; Vanetti,Maria Cristina Dantas; Puschamann,Rolf.
Este estudo avaliou um sistema de biocontrole para inibição de Listeria monocytogenes em couve minimamente processada, objetivando sua segurança durante estocagem sob refrigeração e em condições de abuso de temperatura. O potencial inibitório de bactérias láticas tolerantes ao sal e psicrotróficas contaminantes naturais da couve e Lactobacillus plantarum, Lactobacillus delbrueckii ATCC 9649 e Lactobacillus casei CCT 1465 foram avaliadas contra L. monocytogenes. O isolado de couve identificado como P. acidilactici CCA3 inibiu L. monocytogenes a 10 e 15 °C em ágar MRS e foi selecionado como possível agente de biocontrole. O número de L. monocytogenes na couve minimamente processada aumentou 3,7 e 4,7 ciclos logarítmicos a 5 e 10 °C, respectivamente, após 20...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopreservação; Segurança alimentar; Listeria monocytogenes; Bactérias láticas.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000400013
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Biodiversity of Listeria monocytogenes sensitivity to bacteriocin-producing Carnobacterium strains and application in sterile cold-smoked salmon ArchiMer
Brillet, Anne; Pilet, M; Prevost, H; Bouttefroy, A; Leroi, Francoise.
The aim of this study was to demonstrate the inhibitory capacity of Carnobacterium strains against a collection of Listeria monocytogenes strains in cold-smoked salmon (CSS). Methods and Results: Three bacteriocin-producing strains, Carnobacterium divergens V41, C. piscicola V1 and C. piscicola SF668, were screened for their antilisterial activity against a collection of 57 L. monocytogenes strains selected from the French smoked salmon industry, using an agar spot test. All the Listeria strains were inhibited but three different groups could be distinguished differing in sensitivity to the three Carnobacterium strains. However, C. divergens V41 always had the highest inhibitory effect. The antilisterial capacity was then tested in sterile CSS blocks...
Tipo: Text Palavras-chave: Listeria monocytogenes; Cold smoked salmon; Carnobacterium; Biopreservation; Bacteriocin.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-610.pdf
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Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential BJM
Oliveira,Maíra Maciel Mattos de; Brugnera,Danilo Florisvaldo; Alves,Eduardo; Piccoli,Roberta Hilsdorf.
An experimental model was proposed to study biofilm formation by Listeria monocytogenes ATCC 19117 on AISI 304 (#4) stainless steel surface and biotransfer potential during this process. In this model, biofilm formation was conducted on the surface of stainless steel coupons, set on a stainless steel base with 4 divisions, each one supporting 21 coupons. Trypic Soy Broth was used as bacterial growth substrate, with incubation at 37 ºC and stirring of 50 rpm. The number of adhered cells was determined after 3, 48, 96, 144, 192 and 240 hours of biofilm formation and biotransfer potential from 96 hours. Stainless steel coupons were submitted to Scanning Electron Microscopy (SEM) after 3, 144 and 240 hours. Based on the number of adhered cells and SEM, it was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Biofilm; Biotransfer potential.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100016
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Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence ArchiMer
Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf
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Brazilian goat milk as a source of bacteriocinogenic strains. Repositório Alice
TODOROV, S. D.; VIEIRA, A. D. S.; BURITI, F. C. A.; ROCHA, C. R. C.; LOPES, A. C. de S.; SANTOS, K. M. O. dos; BRUNO, L. M.; FRANCO, B. D. G. de M..
2011
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Leite de cabra; Listeria monocytogenes; Goat milk.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/903179
Registros recuperados: 71
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