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Characterization of 41 microsatellite loci developed from the genome sequence of the poplar rust fungus, Melampsora larici-populina Inra
Xhaard, C.; Andrieux, A.; Halkett, F.; Frey, P..
Forty-one microsatellite markers were developed for the poplar rust fungus Melampsora larici-populina. The genome sequence of M. larici-populina strain 98AG31 was screened in silico for microsatellite loci using Magellan software. Thirty-nine loci, evaluated on a sexual population of 35 M. larici-populina isolates sampled on larch trees in the French Alps, were identified to be highly polymorphic. These markers revealed two to 34 alleles, with an average of 7.23 alleles per locus. The estimated heterozygosity ranged from 0.029 to 0.958 across the 41 microsatellite loci. These markers will be highly useful for population genetics studies of M. larici-populina.
Tipo: Journal Article Palavras-chave: BASIDIOMYCETE; MELAMPSORA LARICI-POPULINA; POPLAR RUST FUNGUS; GENOME SEQUENCE; MICROSATELLITES; M13-TAILED PRIMER METHOD.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e8fd96fd&uri=/notices/prodinra1/2010/10/
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Characterization of two major genetic factors controlling quantitative resistance to Melampsora larici-populina leaf rust in hybrid poplars : strain specificity, field expression, combined effects, and relationship with a defeated qualitative resistance gene Inra
Dowkiw, A.; Bastien, C..
Two genetic factors explain a significant proportion of the variability for quantitative resistance to Melampsora larici-populina leaf rust in a Populus deltoides × P. trichocarpa F1 progeny. One is inherited from P. deltoides and is associated with a defeated qualitative resistance geneR1, and the other, RUS, is inherited from P. trichocarpa. To assess the potential contribution of these two factors for durable resistance breeding, 284 genotypes from this F1 progeny were studied in laboratory experiments with three M. larici-populina strains and in a field experiment under natural inoculum pressure. Results confirmed that both factors can have strong beneficial effects in the laboratory. These effects were strain specific, thus impairing their chances for...
Tipo: Journal Article Palavras-chave: PEUPLIER HYBRIDE; RESISTANCE QUANTITATIVE; FACTEUR GENETIQUE; EFFET RESIDUEL; PEUPLIER HYBRIDE; EXPERIMENTATION EN LABORATOIRE POPULUS DELTOIDE; POPULUS TRICHOCARPA; MELAMPSORA LARICI-POPULINA.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007c4d0fb6b&uri=/notices/prodinra1/2007/04/
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