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Differential activities of four Lactobacillus casei promoters during bacterial transit through the gastrointestinal tracts of human-microbiota-associated mice Inra
Oozeer, R.; Furet, J.P.; Goupil-Feuillerat, N.; Anba, J.; Mengaud, J.; Corthier, G..
In a previous study using fusion of the deregulated lactose promoter lacTp* and reporter genes, we suggested that Lactobacillus casei could initiate de novo protein synthesis during intestinal transit. In order to confirm this finding and extend it to other promoters, we adopted a reverse transcriptase quantitative PCR (RT-QPCR) approach combined with a transcriptional fusion system consisting of luciferase genes under the control of four promoters (ccpA, dlt, ldh, and lacT*) from L. casei DN-114 001. Promoter expression was monitored during cell growth, and variable luciferase activities were detected. In 3-day cultures, all the genetically modified strains survived but without exhibiting luciferase activity. Luciferase mRNA levels determined by RT-QPCR...
Tipo: Journal Article Palavras-chave: HUMAN; MOUSE; GENE EXPRESSION; INTESTINAL MICROBIOTA; FECES; RT-QPCR; PROBIOTIC; GASTROINTESTINAL TRACT.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008ec5c541b&uri=/notices/prodinra1/2008/06/
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Genetic interaction between a maternal factor and the zygotic genome controls the intestine length in PRM/Alf mice Inra
Aubin-Houzelstein, G.; Da Silva; Bellier, S.; Salaun, P.; Montagutelli; Panthier, J..
Genetic interaction between a maternal factor and the zygoticgenome controls the intestine length in PRM/Alf mice. PhysiolGenomics 16: 82–89, 2003. First published October 14, 2003;10.1152/physiolgenomics.00106.2003.—Postoperative managementof small and large bowel resections would be helped by use ofintestinotrophic molecules. Here, we present a mouse inbred straincalled PRM/Alf that is characterized by a selective intestinal lengthening.We show that PRM/Alf intestine is one-third longer comparedwith other inbred strains. The phenotype is acquired mostly during thepostnatal period, before weaning. Its genetic determinism is polygenic,and involves a strong maternal effect. Cross-fostering experimentsrevealed that the dam’s genotype acts synergistically...
Tipo: Journal Article Palavras-chave: INBRED STRAINS; MOUSE; GENOTYPE-ENVIRONMENT INTERACTION; ORGAN DEVELOPMENT; GUT ADAPTATION.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200877868725&uri=/notices/prodinra1/2008/08/
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In vivo anti-tumour activity of ethyl heptyloxyacetate against Ehrlich ascites cells National Institute of Agronomic Research
Townsend, Gordon F.; Brown, William H.; Felauer, Ethel E..
PREVIOUS investigations have shown that whole royal jelly, a fraction from royal jelly (10-hydroxy-2-decenoic acid) and certain closely related dicarboxylic acids, some of which are also found in royal jelly, will inhibit the development of transplantable AKR leukæmia and ascites tumours when added in vitro just prior to transfer. This activity was associated mainly with 9-and 10-carbon straight-chain monocarboxylic acids, particularly decanoic acid in the ethyl ester form. These compounds showed no activity in vivo.
Tipo: Journal Article-postprint Palavras-chave: ROYAL JELLY; HIVE PRODUCT; HEPTYLOXYACETATE; ANTI¬TUMOR; AKR LEUKAEMIA; ASCITE CELL; IN VIVO; MOUSE; GELEE ROYALE; PRODUIT DU RUCHER; HEPTYLOXYACETATE; ANTI-TUMEUR; LEUCEMIE; AKR; IN VIVO; SOURIS.
Ano: 1963 URL: http://hdl.handle.net/2174/437
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Inhibition of myoblast differentiation by Sfrp1 and Sfrp2 Inra
Descamps, S.; Arzouk, H.; Bacou, F.; Bernardi, H.; Fedon, Y.; Gay, S.; Reyne, Y.; Rossano, B.; Levin, J..
Secreted Frizzled-related proteins (Sfrps) areextracellular regulators of Wnt signalling and play importantroles in developmental and oncogenic processes. They areknown to be upregulated in regenerating muscle and inmyoblast cultures but their function is unknown. Here, weshow that the addition of recombinant Sfrp1 or Sfrp2 toC2C12 cell line cultures or to primary cultures of satellitecells results in the inhibition of myotube formation with nosignificant effect on the cell cycle or apoptosis. Even thoughat confluence, treated and untreated cultures are identical inappearance, analyses have shown that, for maximum effect,the cells have to be treated while they are proliferating.Furthermore, removal of Sfrp from the culture mediumduring differentiation...
Tipo: Journal Article Palavras-chave: Sfrp1; Sfrp2; MYOBLAST; PROLIFERATION; DIFFERENTIATION; CELL CULTURE; MOUSE; RABBIT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008942fdfb7&uri=/notices/prodinra1/2009/09/
Registros recuperados: 4
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