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Registros recuperados: 23
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Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide BJMBR
Ramos,M.G.; Rabelo,F.L.A.; Duarte,T.; Gazzinelli,R.T.; Alvarez-Leite,J.I..
We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose- and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The levels of butyrate-induced apoptosis in macrophages were enhanced by co-treatment with 1 µg/ml bacterial lipopolysaccharide (LPS). However, our data indicate that apoptosis induced by butyrate and LPS involves different mechanisms. Thus, LPS-induced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Butyrate; Apoptosis; Caspase; Nitric oxide; Cytokine; Macrophage.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004
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Caractérisation morphologique et fonctionnelle des hémocytes d'Ostrea edulis et de Crassostrea gigas, mollusques bivalves. Etude in vitro de leurs interactions avec le protozoaire Bonamia ostreae (Ascetospora) ArchiMer
Jean Chagot, Dominique.
La morphologie et l'équipement enzymatique des hémocytes de deux espèces d'huîtres, Ostea edulis et Crassostrea gigas ont été étudiés. L'analyse des fonctions des hémocytes dans les mécanismes immunitaires a permis de mettre en évidence leur rôle de macrophage, mais n'a pas fait apparaître de différence interspécifique dans les phénomènes des cytocidie oxygène dépendants, susceptible d'expliquer la permissivité d'Ostrea edulis pour Bonamia ostreae et la résistance de Crassostrea gigas. L'infection in vitro de primocultures d'hémocytes des deux espèces démontre un mécanisme d'entrée par phagocytose, doublé chez Ostrea edulis d'une possible pénétration active. L'analyse des récepteurs impliqués dans la phagocytose (récepteurs pour des opsonines plasmatiques,...
Tipo: Text Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Hémocyte; Morphologie; Macrophage; Caractérisation enzymatiques; Chimioluminescence; Interaction in vitro; Phagocytose; Récepteur.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00210/32102/30559.pdf
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Cissampelos sympodialis Eichl (Menispermaceae) leaf extract induces interleukin-10-dependent inhibition of Trypanosoma cruzi killing by macrophages BJMBR
Alexandre-Moreira,M.S.; Freire-de-Lima,C.G.; Trindade,M.N.; Castro-Faria-Neto,H.C.; Piuvezam,M.R.; Peçanha,L.M.T..
The aqueous fraction of the ethanolic extract (AFL) of Cissampelos sympodialis Eichl (Menispermaceae), popularly known as milona, has been shown to have both immunosuppressive and anti-inflammatory effects. In the present study we investigated the modulation of macrophage antimicrobicidal activity by in vitro treatment with the extract from C. sympodialis. Normal and thioglycolate-elicited mouse peritoneal macrophages were infected in vitro with the protozoan Trypanosoma cruzi DM28c clone. We observed that the AFL (used at doses ranging from 13 to 100 µg/ml) increased T. cruzi growth and induced a 75% reduction in nitric oxide production. This inhibition could be mediated by the stimulation of macrophage interleukin-10 (IL-10) secretion since the in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cissampelos sympodialis; Macrophage; Trypanosoma cruzi; Nitric oxide; Interleukin-10.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200006
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Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major Biol. Res.
LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A.
Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine...
Tipo: Journal article Palavras-chave: Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004
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Effect of cyhalothrin on Ehrlich tumor growth and macrophage activity in mice BJMBR
Quinteiro-Filho,W.M.; Righi,D.A.; Palermo-Neto,J..
Cyhalothrin, a pyrethroid insecticide, induces stress-like symptoms, increases c-fos immunoreactivity in the paraventricular nucleus of the hypothalamus, and decreases innate immune responses in laboratory animals. Macrophages are key elements in cellular immune responses and operate at the tumor-host interface. This study investigated the relationship among cyhalothrin effects on Ehrlich tumor growth, serum corticosterone levels and peritoneal macrophage activity in mice. Three experiments were done with 10 experimental (single gavage administration of 3.0 mg/kg cyhalothrin daily for 7 days) and 10 control (single gavage administration of 1.0 mL/kg vehicle of cyhalothrin preparation daily for 7 days) isogenic BALB/c mice in each experiment. Cyhalothrin i)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyhalothrin; Corticosterone; Macrophage; Ehrlich tumor; Innate immunity; HPA axis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000006
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Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization BJMBR
Persechini,P.M.; Bisaggio,R.C.; Alves-Neto,J.L.; Coutinho-Silva,R..
The effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ATP; P2Z purinoceptor; Macrophage; Monocyte; T cell; Permeabilization.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004
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Galectin-1, an alternative signal for T cell death, is increased in activated macrophages BJMBR
Rabinovich,G.A.; Riera,C.M.; Sotomayor,C.E..
Galectin-1 belongs to an evolutionarily conserved family of animal ß-galactoside-binding proteins, which exert their functions by crosslinking the oligosaccharides of specific glycoconjugate ligands. During the past decade, attempts to identify the functional role of galectin-1 suggested participation in the regulation of the immune response. Only in the last few years has the molecular mechanism involved in these properties been clearly elucidated, revealing a critical role for galectin-1 as an alternative signal in the generation of T cell death. In the present study we will discuss the latest advances in galectin research in the context of the regulation of the immune response, not only at the central level but also at the periphery. Moreover, we will...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galectin-1; Apoptosis; Immunomodulation; Macrophage.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500009
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Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages OAK
NISHIKAWA, Yoshifumi; IBRAHIM, Hany M.; KAMEYAMA, Kyohko; SHIGA, Ikumi; HIASA, Jun; XUAN, Xuenan; 西川, 義文; 玄, 学南.
The intracellular protozoan Toxoplasma gondii lacks the ability to synthesize sterol and scavenges cholesterol from the lowdensity lipoprotein receptor (LDLR) pathway of its host to facilitate replication. Sterol biosynthesis inhibitors, however, have a demonstrated anti-Toxoplasma effect. In this study, we examined the host mevalonate pathway as a novel source of cholesterol for T. gondii and its effects on parasite growth in macrophages. Parasite growth did not significantly change in the absence of LDLR or when LDL was exogenously supplemented. Lovastatin and compactin, both inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase in the mevalonate pathway, significantly inhibited T. gondii growth in both wild-type and LDLR-knockout macrophages....
Palavras-chave: Cholesterol; Lipid metabolism; Macrophage; Parasitology; Toxoplasma gondii.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3583
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Immunology and immunopathology of African trypanosomiasis Anais da ABC (AABC)
Vincendeau,Philippe; Bouteille,Bernard.
Major modifications of immune system have been observed in African trypanosomiasis. These immune reactions do not lead to protection and are also involved in immunopathology disorders. The major surface component (variable surface glycoprotein,VSG) is associated with escape to immune reactions, cytokine network dysfunctions and autoantibody production. Most of our knowledge result from experimental trypanosomiasis. Innate resistance elements have been characterised. In infected mice, VSG preferentially stimulates a Th 1-cell subset. A response of <FONT FACE=Symbol>gd</FONT> and CD8 T cells to trypanosome antigens was observed in trypanotolerant cattle. An increase in CD5 B cells, responsible for most serum IgM and production of autoantibodies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosome; African trypanosomiasis; Immunology; Macrophage; Lymphocytes; Nitric oxide; Cytokine; Autoantibodies.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400004
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Immunoregulatory and anti-inflammatory effects of n-3 polyunsaturated fatty acids BJMBR
Calder,P.C..
1. Fish oils are rich in the long-chain n-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Linseed oil and green plant tissues are rich in the precursor fatty acid, <FONT FACE="Symbol">a</font>-linolenic acid (18:3n-3). Most vegetable oils are rich in the n-6 PUFA linoleic acid (18:2n-6), the precursor of arachidonic acid (20:4n-6). 2. Arachidonic acid-derived eicosanoids such as prostaglandin E2 are pro-inflammatory and regulate the functions of cells of the immune system. Consumption of fish oils leads to replacement of arachidonic acid in cell membranes by eicosapentaenoic acid. This changes the amount and alters the balance of eicosanoids produced. 3. Consumption of fish oils diminishes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lymphocyte; Macrophage; Immune system; Inflammation; Fish oil; Polyunsaturated fatty acid; Cytokine; Eicosanoid.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400002
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Influence of acute renal failure on the mononuclear phagocytic system BJMBR
Sousa,V.R.A.; Sousa,A.A.; Petroianu,A.; Simal,C.J.R.; Barbosa,A.J.A..
Several studies show the ability of macrophages to remove particles injected into the bloodstream. This function seems to be increased in the presence of acute renal failure. The objective of the present study was to assess the phagocytic function of the main organs (spleen, liver and lung) of the mononuclear phagocytic system in renal and postrenal failures. Fifteen rats (250-350 g) were divided into three groups (N = 5): group I - control; group II - ligature of both ureters, and group III - bilateral nephrectomy. On the third postoperative day, all animals received an iv injection of 1 ml/kg 99mTc sulfur colloid. Blood samples were collected for the assessment of plasma urea, creatinine, sodium, and potassium concentrations and arterial gasometry....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renal failure; Reticuloendothelial system; Phagocytosis; Macrophage; Scintigraphy.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900010
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Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis Genet. Mol. Biol.
Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013
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Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish Anais da ABC (AABC)
KWON,DA HYE; JEONG,JIN WOO; CHOI,EUN OK; LEE,HYE WON; LEE,KI WON; KIM,KI YOUNG; KIM,SUNG GOO; HONG,SU HYUN; KIM,GI-YOUNG; PARK,CHEOL; HWANG,HYE-JIN; SON,CHANG-GUE; CHOI,YUNG HYUN.
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mori folium; Inflammation; ROS; Macrophage; Zebrafish.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661
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Outward potassium current oscillations in macrophage polykaryons: extracellular calcium entry and calcium-induced calcium release BJMBR
Saraiva,R.M.; Masuda,M.O.; Oliveira-Castro,G.M..
Outward current oscillations associated with transient membrane hyperpolarizations were induced in murine macrophage polykaryons by membrane depolarization in the absence of external Na+. Oscillations corresponded to a cyclic activation of Ca2+-dependent K+ currents (IKCa) probably correlated with variations in intracellular Ca2+ concentration. Addition of external Na+ (8 mM) immediately abolished the outward current oscillations, suggesting that the absence of the cation is necessary not only for their induction but also for their maintenance. Oscillations were completely blocked by nisoldipine. Ruthenium red and ryanodine reduced the number of outward current cycles in each episode, whereas quercetin prolonged the hyperpolarization 2- to 15-fold. Neither...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrophage; Macrophage polykaryon; Ca2+-dependent K+ current; Membrane potential oscillations; Calcium-induced calcium release; Ca2+ ATPase.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100015
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Phagocytosis by macrophages mediated by receptors for denatured proteins - dependence on tyrosine protein kinases BJMBR
Hespanhol,M.R.; Mantovani,B..
Previous studies have demonstrated that some components of the leukocyte cell membrane, CR3 (Mac-1, CD11b/CD18) and p150/95, are able to bind to denatured proteins. Thus, it is of interest to know which effector functions of these cells can be triggered by these receptors when they interact with particles or surfaces covered with denatured proteins. In the present study we analyzed their possible role as mediators of phagocytosis of red cells covered with denatured bovine serum albumin (BSA) by mouse peritoneal macrophages. We observed that a) macrophages are able to recognize (bind to) these red cells, b) this interaction can be inhibited by denatured BSA in the fluid phase, c) there is no phagocytosis of these particles by normal macrophages, d)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phagocytosis; Denatured proteins; Macrophage; SS2 Integrins; Tyrosine protein kinases.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300015
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Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method Biol. Res.
Park,Hee-Sook; Choi,Hye-Young; Kim,Gun-Hee.
BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 &#956;g/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on...
Tipo: Journal article Palavras-chave: Chlorogenic acid; Inflammation; Ligularia fischeri; Macrophage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100063
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Role of nitric oxide and superoxide in Giardia lamblia killing BJMBR
Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (&gt;90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015
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Salmonella enterica serovar Typhi plasmid pR ST98 enhances intracellular bacterial growth and S. typhi-induced macrophage cell death by suppressing autophagy BJID
He,Peiyan; Wu,Shuyan; Chu,Yuanyuan; Yang,Yanru; Li,Yuanyuan; Huang,Rui.
OBJECTIVES: Plasmid pR ST98 is a hybrid resistance-virulence plasmid isolated from Salmonella enterica serovar Typhi (S. typhi). Previous studies demonstrated that pR ST98 could enhance the virulence of its host bacteria. However, the mechanism of pR ST98-increased bacterial virulence is still not fully elucidated. This study was designed to gain further insight into the roles of pR ST98 in host responses. METHODS: Human-derived macrophage-like cell line THP-1 was infected with wild-type (ST8), pR ST98-deletion (ST8-ΔpR ST98), and complemented (ST8-c-pR ST98) S. typhi strains. Macrophage autophagy was performed by extracting the membrane-unbound LC3-I protein from cells, followed by flow cytometric detection of the membrane-associated fraction of LC3-II....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmid pR ST98; Macrophage; Autophagy; Intracellular bacterial growth; Cell death.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300008
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Simvastatin acts as an inhibitor of interferon gamma-induced cycloxygenase-2 expression in human THP-1 cells, but not in murine RAW264.7 cells Biocell
Lee,Chang Seok; Shin,Yong Jae; Won,Cheolhee; Lee,Yun-Song; Park,Chung-Gyu; Ye,Sang-Kyu; Chung,Myung-Hee.
Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNgamma)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNgamma-induced COX-2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNgamma-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNgamma-induced COX-2 expression in murine RAW264.7...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monocyte; Macrophage; INFgamma; STAT1/3; SOCS1/3.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200005
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Species-specific Interaction of Leishmania Promastigotes with Macrophages : Effect of Previous Incubation of the Host Cells with Enzymes, Monosaccharides and Lectin OAK
Ghosh, Dilip Kumar.
Palavras-chave: Leishmaniases; Leishmania; Macrophage; Ligands; Receptors.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/206
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