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Registros recuperados: 13
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A multibiomarker approach on the Atlantic tomcod (Microgadus tomcod) in the St. Lawrence Estuary ArchiMer
Dupuy, Celie; Couillard, Catherine M.; Laroche, Jean; Nellis, Pierre; Brousseau, Pauline; Fournier, Michel.
A multibiomarker approach was developed on juvenile Atlantic tomcod (Microgadus tomcod) to evaluate the pertinence of this approach for low-cost screening assessment of the environmental quality of various coastal sites within estuaries. Several biometric indices and biomarkers (ethoxyresorufin-O-deethylase (EROD) activity, metallothionein concentration, and immune responses) were investigated on immature and maturing tomcods (a parts per thousand currency sign31 months) collected in four environmentally contrasted sites in the St. Lawrence Estuary (SLE). Simultaneous examination of various age classes provides the opportunity to detect short-term responses in sensitive young-of-the-year fish (e.g., EROD induction) and longer-time effects associated with...
Tipo: Text Palavras-chave: Biometric indexes; EROD; Metallothionein; Immunotoxicity; Pollution; Fish.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23578/21501.pdf
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Bioaccumulation of metals in sediment elutriates and their effects on growth, condition index, and metallothionein contents in oyster larvae ArchiMer
Geffard, A; Geffard, O; Amiard, J; His, Edouard; Amiard Triquet, C.
The bioavailability of Cd, Cu, Zn, and Pb from two metal-contaminated sediments (Bidassoa and Dunkerque) was studied using Crassostrea gigas larvae exposed to sediment elutriates. The metal contents within the sediments, the larvae and larval growth, the condition index, and the induction of metallothionein in the larvae were measured. The larval growth and condition index were only affected after exposure to the highest elutriates concentration (5 to 25%) from the most contaminated sediment (Dunkerque). Bioaccumulation of all metals was observed in larvae exposed to Dunkerque elutriatre; only Cu bioaccumulation was observed in the Bidassoa elutriate. The results from larvae exposed to both sediment elutriates show a strong correlation between...
Tipo: Text Palavras-chave: Metallothionein; Metal bioaccumulation; Larval growth; Crassostrea gigas larvae; Sediment elutriates.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4748.pdf
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Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi. Biol. Res.
MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO.
Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,...
Tipo: Journal article Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007
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Gene expression profiling in wild-type and metallothionein mutant fibroblast cell lines Biol. Res.
ARMENDÁRIZ,ÁNGELA D; OLIVARES,FELIPE; PULGAR,RODRIGO; LOGUINOV,ALEX; CAMBIAZO,VERÓNICA; VULPE,CHRISTOPHER D; GONZÁLEZ,MAURICIO.
The role of metallothioneins (MT) in copper homeostasis is of great interest, as it appears to be partially responsible for the regulation of intracellular copper levels during adaptation to extracellular excess of the metal. To further investigate a possible role of MTs in copper metabolism, a genomics approach was utilized to evaluate the role of MT on gene expression. Microarray analysis was used to examine the effects of copper overload in fibroblast cells from normal and MT I and II double knock-out mice (MT-/-). As a first step, we compared genes that were significantly upregulated in wild-type and MT-/- cells exposed to copper. Even though wild-type and mutant cells are undistinguishable in terms of their morphological features and rates of growth,...
Tipo: Journal article Palavras-chave: Copper homeostasis; Metallothionein; Microarray.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100015
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Metal bioaccumulation and metallothionein concentrations in larvae of Crassostrea gigas ArchiMer
Damiens, Gautier; Mouneyrac, Catherine; Quiniou, Francoise; His, Edouard; Gnassia Barelli, Mauricette; Romeo, Michèle.
Larval stages of bivalve molluscs are highly sensitive to pollutants. Oysters from a hatchery from Normandy (English Channel) were induced to spawn, and fertilized eggs were exposed to copper or cadmium for 24 h. Metal accumulation (from 0.125 to 5 mu g Cu L-1 and from 25 to 200 mu g Cd L-1) and MT concentrations were measured in larvae. Compared to controls, larvae accumulated copper and cadmium with an increase in MT concentrations particularly with cadmium (i.e. 130.96 ng Cu (mg protein)(-1) and 12.69 mu g MT (mg protein)(-1) at 1 mu g Cu L-1 versus 23.19 ng Cu (mg protein)(-1) and 8.92 mu g MT (mg protein)(-1) in control larvae; 334.3 ng Cd (mg protein)(-1) and 11.70 mu g MT (mg protein)(-1) at 200 mu g Cd L-1 versus 0.87 ng Cd (mg protein)(-1) and...
Tipo: Text Palavras-chave: Bidassoa estuary; Arcachon bay; Metallothionein; Copper; Cadmium; Crassostrea gigas larvae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1229.pdf
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Metallothionein: An Overview on its Metal Homeostatic Regulation in Mammals International Journal of Morphology
Sakulsak,Natthiya.
Metallothionein (MT) is a ubiquitous protein with a low molecular weight of 6-7 kDa weight and it was first identified in the kidney cortex of equines as a cadmium (Cd)-binding protein responsible for the natural accumulation of Cd in the tissue. The mammalian MT contains 61 to 68 amino acid residues, in which 18 to 23 cysteine residues are present. The expression of MT starts by binding of metal transcription factor-1 (MTF-1) to the regulative region of MT gene called metal responsive elements (MREs). The induction of MT through the MREs region can be initiated by several metal ions such as zinc (Zn), copper (Cu) and Cd. However, Zn is the only heavy metal which can reversibly and directly activate the DNA-binding activity of MTF-1. In mammals four types...
Tipo: Journal article Palavras-chave: Metallothionein; Cadmium; Zinc.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300039
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Metallothionein and lipid peroxidation as markers to assess health status of chronically oiled Magellanic penguins in Argentina Acta Toxicol. Argent.
Romero,María Belén; Polizzi,Paula; Chiodi,Leila; Robles,Alicia; Rodríguez Heredia,Sergio; Gerpe,Marcela.
Chronic oiling affects seabirds, with short (individual survival) and long-term (dynamics of population) effects. Magellanic penguin is the most affected species in Argentina. The aim was to evaluate the use of blood biochemical-molecular biomarkers (MT and TBARs) to assess health status of Magellanic penguin exposed to chronic environmental oiling, in addition with routine hematological analyses (differential WBC count and PCV). Oiled specimens presented significant higher RBC metallothionein and TBARs concentrations than those from shelter; while routine hematological parameters were within normal levels for penguins and did not show any difference between both groups. These results demonstrated the higher sensibility of both biomarkers, evidencing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic oiling; Metallothionein; Lipid peroxidation; Magellanic penguin.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432015000100002
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Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels ArchiMer
Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine.
We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase...
Tipo: Text Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf
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Phenotypic and genetic differentiation in young-of-the-year common sole (Solea solea) at differentially contaminated nursery grounds ArchiMer
Guinand, Bruno; Durieux, Eric D. H.; Dupuy, Celie; Cerqueira, Frederique; Begout, Marie-laure.
Growth-related characters, condition factor, and genetic differentiation were investigated for a single cohort of young-of-the-year (YOY) sole within and among nurseries with differing levels of heavy metals (Cd, Cu and Zn) contamination in the two Charentais Straits, Bay of Biscay, France. Analyses were performed when individuals recruited (May), then after a full summer spent in each nursery (October). Levels of phenotypic and genetic diversity were compared, together with genetic differentiation at a candidate metallothionein (MT) locus and three putatively neutral microsatellite loci. No phenotypic or genetic differentiation was detected among nurseries in May, but significant variation at each phenotypic trait and at the multilocus level in October (P...
Tipo: Text Palavras-chave: Solea solea; Nursery; Bay of Biscay; Metallothionein.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14392/11694.pdf
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Relationships between metal bioaccumulation and metallothionein levels in larvae of Mytilus galloprovincialis exposed to contaminated estuarine sediment elutriate ArchiMer
Geffard, A; Geffard, Olivier; His, Edouard; Amiard, Jc.
The bioavailability of Cd, Cu and Zn from a metal-contaminated sediment was studied using Mytilus galloprovincialis embryos and larvae exposed to sediment elutriates, The elutriate concentrations tested were always lower than the threshold at which abnormal larval development appears. The Cd, Cu and Zn contents of the sediment, of the elutriate and of the larvae, as well as the larval growth, the condition index (CI), and the induction of metallothionein (NIT) in the larvae were measured. The Cl was only affected after 96 h of exposure, principally at the highest elutriate concentration (25%, corresponding to 0.1435 mug Cd l(-1) and 14 mug Zn l(-1)). Cd, Cu and Zn bioaccumulation was observed (48 and 96 h), in whole larvae as well as in the cytosolic...
Tipo: Text Palavras-chave: Sediment elutriate; Mytilus galloprovincialis larvae; Metal bioaccumulation; Metallothionein.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00000/10119/7598.pdf
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Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers ArchiMer
Gueguen, Yannick; Saulnier, Denis; Santini, Adrien; Magre, Kevin; Garen, Pierre; Bernagout, Solene; Nohl, Marine; Bouisset, Patrick; Helme, Herehia; Planes, Serge; Le Moullac, Gilles.
This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM,...
Tipo: Text Palavras-chave: Pinctada margaritifera; French Polynesia; Cadmium; Chromium; Gene expression; Metallothionein.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00376/48707/49146.pdf
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Seasonal changes in mRNA encoding for cell stress markers in the oyster Crassostrea gigas exposed to radioactive discharges in their natural environment ArchiMer
Farcy, Emilie; Voiseux, Claire; Lebel, Jean-marc; Fievet, Bruno.
The North Cotentin area (Normandy, France) hosts several nuclear facilities among which the AREVA reprocessing plant of La Hague is responsible for controlled discharges of liquid radioactive wastes into the marine environment. The resulting increase in radioactivity is very small compared to natural radioactivity. However, concerns about environment protection prompted the scientific community to focus on the effects of the chronic exposure to low concentrations of radionuclides in non-human biota. This study contributes to the evaluation of the possible impact of radioactive discharges on the oyster Crassostrea gigas in the field. Real-time polymerase chain reaction was used to quantify the expression levels of genes involved in cell stress in the...
Tipo: Text Palavras-chave: Crassostrea gigas; Superoxide dismutase; Metallothionein; Heat shock protein; MRNA; Seasonal variations; Radioactive discharges.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2601.pdf
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Subcellular distribution of Ag, Cd, Co, Cu, Fe, Mn, Ph, and Zn in the digestive gland of the common cuttlefish Sepia officinalis ArchiMer
Bustamante, Paco; Bertrand, M; Boucaud Camou, E; Miramand, Pierre.
The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic...
Tipo: Text Palavras-chave: Sepia officinalis; Chromatography; Metallothionein; Detoxification; Trace element; Metal; Cephalopod.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2532.pdf
Registros recuperados: 13
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