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Developing an in vitro optimized protocol to sweet potato landraces conservation Agronomy
Vettorazzi, Renato Gobbi; Carvalho, Virginia Silva; Sudré, Cláudia Pombo; Rodrigues, Rosana.
This study aimed to develop a protocol for the in vitro conservation of 30 accessions of sweet potato collected in the northern region of Rio de Janeiro State, Brazil, aiming to extend the period between subcultures for this species. An in vitro minimal growth experiment was established with accession UENF 1931 in a factorial arrangement consisting of four concentrations of mineral salts in MS medium and four concentrations of sucrose, at two temperatures. The others accessions were grown in medium containing 100% MS salts and 2% sucrose, which was the medium that allowed the in vitro conservation of accession UENF 1931 for a longer time period. For this second experiment, 30 accessions of sweet potato and two temperatures were used. Plant height, number...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 Ipomoea batatas; Minimal growth; Germplasm bank; Maintenance of diversity; Fitotecnia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32700
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Short-term storage in vitro and large-scale propagation of grapevine genotypes. Repositório Alice
SILVA, R. de C; LUIS, Z. G.; PEREIRA, J. E. S..
The objective of this work was to evaluate the large?scale propagation of grapevine genotypes after short?term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short?term storage, the shoots were propagated in up to five successive subcultures, to assess the large?scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short?term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ex situ conservation; In vitro conservation; Minimal growth; Vitis.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/930351
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