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Registros recuperados: 17
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A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee BABT
Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
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Antibodies against electronegative LDL inhibit atherosclerosis in LDLr-/- mice BJMBR
Grosso,D.M.; Ferderbar,S.; Wanschel,A.C.B.A.; Krieger,M.H.; Higushi,M.L.; Abdalla,D.S.P..
In order to determine the effect of antibodies against electronegative low-density lipoprotein LDL(-) on atherogenesis, five groups of LDL low receptor-deficient (LDLr-/-) mice (6 per group) were immunized with the following antibodies (100 µg each): mouse anti-LDL(-) monoclonal IgG2b, rabbit anti-LDL(-) polyclonal IgG or its Fab fragments and mouse irrelevant monoclonal IgG and non-immunized controls. Antibodies were administered intravenously one week before starting the hypercholesterolemic diet (1.25% cholesterol) and then every week for 21 days. The passive immunization with anti-LDL(-) monoclonal IgG2b, polyclonal antibody and its derived Fab significantly reduced the cross-sectional area of atherosclerotic lesions at the aortic root of LDLr-/- mice...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minimally modified LDL; Passive immunization; Monoclonal antibody; Atherogenesis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200007
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Antigenic Diversity among Oocysts of Clinical Isolates of Cryptosporidium parvum OAK
Griffin, K.; Matthai, E.; Hommel, M.; Weitz, J. C.; Baxby, D.; Hart, C. A..
A panel of monoclonal antibodies has been produced against the protozoan parasite Cryptosporidium parvum which is a major cause of diarrhoeal disease in man and other animals. C. parvum oocysts from both a human and a bovine case of cryptosporidiosis were used as immunogens. A total of 11 different monoclonal antibodies were obtained which could bind to formalin-fixed oocysts. One was IgA but the remainder were all of IgM isotype. The reactivity of these monoclonal antibodies against a series of C. parvum oocysts obtained from 25 patients in Chile was examined using indirect immuno-fluorescence. Although a mixture of all of the 11 antibodies would have detected oocysts in each of the samples, no one monoclonal antibody recognised all oocysts. Each antibody...
Palavras-chave: Cryptosporidium parvum; Monoclonal antibody; Antigenic diversity.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/169
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Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus Arq. Bras. Med. Vet. Zootec.
Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W..
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001
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Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates BJM
Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes.
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004
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Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum BJMBR
Oshiro,T.M.; Rafael,A.; Enobe,C.S.; Fernandes,I.; Macedo-Soares,M.F..
The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated by gel filtration from an extract of adult worms were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells and the cloned hybrid cells obtained were screened to determine the specificity of secreted antibodies. Three monoclonal antibodies named MAIP-1, MAIP-2 and MAIP-3 were selected and were shown to react with different epitopes...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ascaris suum; Immunosuppression; Immunomodulation; Monoclonal antibody.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200009
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Development of Monoclonal Antibodies That Target 1-Cys Peroxiredoxin and Differentiate Plasmodium falciparum from P. vivax and P. knowlesi OAK
Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎.
Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising...
Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859
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Development of two potential diagnostic monoclonal antibodies against human cytomegalovirus glycoprotein B BJMBR
Jun,Fan; Minhuan,Li; Yadan,Ma; Yaping,Huang; Hanying,Liang; Jianhua,Hu; Hangping,Yao; Weihang,Ma.
Human cytomegalovirus glycoprotein B (gB) represents a target for diagnosis and treatment in view of the role it plays in virus entry and spread. Nevertheless, to our knowledge, rare detection of a gB antigen has been reported in transplant patients and limited information is available about diagnostic gB monoclonal antibodies (mAbs). Our aim was to develop gB mAbs with diagnostic potential. Hydrophilic gB peptides (ST: amino acids 27-40, SH: amino acids 81-94) of favorable immunogenicity were synthesized and used to immunize BALB/c mice. Two mAbs, named ZJU-FH6 and ZJU-FE6, were generated by the hybridoma technique and limited serial dilution and then characterized by indirect ELISA, Western blotting, immunoprecipitation, and immunohistochemical staining....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human cytomegalovirus; Glycoprotein B; Monoclonal antibody; Antigen detection; Transplantation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700004
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Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals OAK
Furuoka, Hidefumi; Yabuzoe, Atushi; Horiuchi, Motohiro; Tagawa, Yuichi; Yokoyama, Takashi; Yamakawa, Yoshio; Shinagawa, Morikazu; Sata, Tetsutaro.
For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could...
Palavras-chave: Prion protein; Immunohistochemistry; Antigen retrieval; Autoclaving; Monoclonal antibody; CREUTZFELDT-JAKOB-DISEASE; MONOCLONAL-ANTIBODIES; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; PROTOCOLS; FIXATION; DEPOSITS; PLAQUES; BRAIN; PRP.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/712
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Eimeria tenella : Localization of Cross-reacting Epitopes in Rhoptry, Nucleus, and Host Cell Nucleus during Parasite-host Cell Interactions by a Monoclonal Antibody in PCKC Culture OAK
Greif, Gisela; Entzeroth, Rolf.
Palavras-chave: Eimeria tenella; Monoclonal antibody; Rhoptry.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/238
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Evaluation of an anti-carcinoembryonic monoclonal antibody suitable for immunoscintigraphy BJMBR
Moraes,D.W.; Lima,E.N.P.; Prado,I.B.; Carneiro,C.R.W..
An anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb 6D1.1) was evaluated in vitro and in vivo to determine its suitability as a tracer for immunoscintigraphy of colorectal carcinomas. Determination of mAb affinity for CEA showed a constant of association of 0.63 ± 0.11 x 109 M-1. Binding of technetium-99m (99mTc)-6D1.1, labeled by a direct method, to human cultured lineages was highly specific. Binding to only CEA-positive LS-174T cells resulted in a saturable curve inhibited by pre-incubation with unlabeled mAb. No binding at all was observed for the human lineages MeWo (melanoma) or ZR75-30 (breast carcinoma), neither of them expressing CEA cells. Intravenous injection of 99mTc-6D1.1 into nude mice xenografted with human LS-174T tumors...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monoclonal antibody; Carcinoembryonic antigen; Colorectal carcinoma; Immunoscintigraphy; Technetium-99m.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800006
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Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat BJM
Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães.
In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Monoclonal antibody; Policlonal antibody; ELISA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027
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Monoclonal antibody against the surface of Alexandrium minutum used in a whole-cell ELISA ArchiMer
Gas, F; Pinto, L; Baus, B; Gaufres, L; Crassous, Marie-pierre; Compere, Chantal; Quemeneur, E.
Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Alexandrium minutum, which causes paralytic shellfish poisoning, is occurring with increasing frequency along European coasts. Current regulatory methods to analyze environmental samples are tedious and time consuming because they require taxonomists and involve animal experiments. New rapid detection methods, such as immunoassays, are needed to ensure a fast alert system and for field studies of algal ecodynamics. Rat monoclonal antibodies were raised and selected for their ability to specifically recognize a surface antigen for the A. minutum strain AM89BM from the Bay of Morlaix, France. A whole-cell ELISA was designed, leading to the selection of...
Tipo: Text Palavras-chave: Monoclonal antibody; Harmful algal bloom; Enzyme linked immunosorbent assay; Dinoflagellate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4647.pdf
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Pertuzumab and trastuzumab: the rationale way to synergy Anais da ABC (AABC)
RICHARD,SANDRINE; SELLE,FRÉDÉRIC; LOTZ,JEAN-PIERRE; KHALIL,AHMED; GLIGOROV,JOSEPH; SOARES,DANIELE G..
ABSTRACT It has now been 15 years since the HER2-targeted monoclonal antibody trastuzumab was introduced in clinical and revolutionized the treatment of HER2-positive breast cancer patients. Despite this achievement, most patients with HER2-positive metastatic breast cancer still show progression of their disease, highlighting the need for new therapies. The continuous interest in novel targeted agents led to the development of pertuzumab, the first in a new class of agents, the HER dimerization inhibitors. Pertuzumab is a novel recombinant humanized antibody directed against extracellular domain II of HER2 protein that is required for the heterodimerization of HER2 with other HER receptors, leading to the activation of downstream signalling pathways....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast cancer; Dimerization; HER2/3; Monoclonal antibody; Pertuzumab; Trastuzumab.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200565
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Preparation of monoclonal antibodies against gamma-type phospholipase A2 inhibitors and immunodetection of these proteins in snake blood J. Venom. Anim. Toxins incl. Trop. Dis.
Li,Jingjing; Xiong,Ying; Sun,Shimin; Yu,Lehan; Huang,Chunhong.
Abstract Background: The gamma-type phospholipase A2 inhibitor (ΡLIγ) is a natural protein commonly found in snake serum, which can neutralize pathophysiological effects of snake venom phospholipases A2. Therefore, this protein is a potential candidate to the development of a novel antivenom. To the best of our knowledge, there is no antibody currently available for PLIγ identification and characterization. Methods: Bioinformatics prediction of epitope using DNAStar software was performed based on the sequence of Sinonatrix annularis PLIγ (SaPLIγ). The best epitope 151CPVLRLSNRTHEANRNDLIKVA172 was chosen and synthesized, and then conjugated to keyhole limpet hemocyanin and bovine serum albumin for use as an immunogen and plate-coating antigen,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monoclonal antibody; Phospholipase A2 inhibitor; Epitope prediction.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100314
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Production of monoclonal antibodies against conserved components of infectious bronchitis virus Arq. Bras. Med. Vet. Zootec.
Souza,C.M.; Rocha,F.R.T.; Martins,N.R.S.; Resende,J.S.; Jorge,M.A.; Rampinelli,A.P..
Murine hybridomas producing IgG1 monoclonal antibodies (Mabs) against N and S2 proteins (53KDa and 82KDa, respectively) from avian infection bronchitis virus (IBV) strain M41 were generated by the fusion of a myeloma cell line (Sp2/0-Ag14) with spleen cells from Balb/c mice previously immunized with whole virus IBV M41. Post-fusion screening criterion was by ELISA and 36 positive hybrids were generated after fusions. Two hybrids specific to N (N3F10) and S2 (S12B2) proteins from M41 (serotype Massachusetts) were selected by western blotting. These Mabs recognized the Ark-99 (serotype Arkansas) and A5968 (serotype Connecticut) IBV strains in addition to M41. By ELISA, the Mab against the S2 (S12B2) recognized all reference and Brazilian strains (M41, SE-17,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Monoclonal antibody; Infectious bronchitis; Nucleoprotein; Spike glicoprotein S2.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500002
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Radiolabeling and biodistribution of monoclonal antibody (MAb) anti-CD20 with iodine-131 BABT
Akanji,Akinkunmi Ganiyu; Muramoto,Emiko; Caldeira Filho,José de Souza; Couto,Renata Martinussi; Araújo,Elaine Bortoletti de.
Radioactive isotopes of iodine, mainly iodine-131 have been broadly used in many monoclonal antibody radioiodination settings, employing different methods. In this study, using a Chloramine-T procedure, the influence of incubation time, CT mass, and Ab/activity ratio on the radiochemical yield of the anti-CD20 antibody labeled with iodine-131 was observed. Radiochemical yield was > 97 %, employing different Chloramine-T masses, independently of incubation time. Radiochemical purity was above 99 % after purification of the labeled compound. The relationship between Ab mass and radioactivity was tested and no difference was observed when 90.6 MBq (2.45mCi) of activity was incorporated in the Ab-mass range studied. Biodistribution study in normal Swiss...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monoclonal antibody; Radioioactivity; Anti-CD20; Radioimmunotherapy.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700010
Registros recuperados: 17
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