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Purification and characterization of infectious myonecrosis virus of penaeid shrimp ArchiMer
Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D.
The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)...
Tipo: Text Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf
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