Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 13
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling BJMBR
Pan,Q.; Yang,X.-H.; Cheng,Y.-X..
Angiotensin II (Ang II) plays a crucial role in the pathogenesis of renal diseases. The objective of the present study was to investigate the possible inflammatory effect of Ang II on glomerular endothelial cells and the underlying mechanism. We isolated and characterized primary cultures of rat glomerular endothelial cells (GECs) and observed that Ang II induced the synthesis of monocyte chemoattractant protein-1 (MCP-1) in GECs as demonstrated by Western blot. Ang II stimulation, at concentrations ranging from 0.1 to 10 µm, of rat GECs induced a rapid increase in the generation of reactive oxygen species as indicated by laser fluoroscopy. The level of p47phox protein, an NAD(P)H oxidase subunit, was also increased by Ang II treatment. These effects of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ang II; Glomerular endothelial cells; MCP-1; Reactive oxygen species; AT1 receptor blocker; NF-κB.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600009
Imagem não selecionada

Imprime registro no formato completo
Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro BJMBR
Zhang,Y.; Han,H.; Sun,L.; Qiu,H.; Lin,H.; Yu,L.; Zhu,W.; Qi,J.; Yang,R.; Pang,Y.; Wang,X.; Lu,G.; Yang,Y..
Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Cytotoxicity of PMM-034 on RD cells was determined using WST-1 assay. Dose- and time-dependent effects of PMM-034 on EV71 replication in RD cells were determined using plaque reduction assay. mRNA expression levels of EV71/VP1 and pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EV71; VP1; Shikonin ester derivatives PMM-034; Rhabdomyosarcoma cells; NF-κB.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000602
Imagem não selecionada

Imprime registro no formato completo
CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway BJMBR
Long,T.Y.; Jing,R.; Kuang,F.; Huang,L.; Qian,Z.X.; Yang,T.L..
Myocardial ischemia is a major cause of death and remains a disease with extremely deficient clinical therapies and a major problem worldwide. Cold inducible RNA-binding protein (CIRBP) is reported to be involved in multiple pathological processes, including myocardial ischemia. However, the molecular mechanisms of myocardial ischemia remain elusive. Here, we first overexpressed CIRBP by transfection of pc-CIRBP (pcDNA3.1 containing coding sequenced for CIRBP) and silenced CIRBP by transfection of small interfering RNA targeting CIRBP (siCIRBP). pcDNA3.1 and the negative control of siCIRBP (siNC) were transfected into H9C2 cells to act as controls. We then constructed a cell model of myocardial ischemia through culturing cells in serum-free medium with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myocardial ischemia; CIRBP; NF-κB; Cell proliferation; Cell apoptosis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400602
Imagem não selecionada

Imprime registro no formato completo
Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults BJMBR
Kawamoto,E.M.; Gleichmann,M.; Yshii,L.M.; Lima,L. de Sá; Mattson,M.P.; Scavone,C..
Wnt proteins are involved in tissue development and their signaling pathways play an important role during embryogenesis. Wnt signaling can promote cell survival, which is beneficial for neurons, but could also lead to tumor development in different tissues. The present study investigated the effects of a Wnt protein on the susceptibility of a neural tumor cell line (PC12 cells) to the cytotoxic compounds ferrous sulfate (10 mM), staurosporine (100 and 500 nM), 3-nitropropionic acid (5 mM), and amyloid β-peptide (Aβ25-35; 50 µM). Cells (1 x 10(6) cells/mL) were treated with the Wnt-3a recombinant peptide (200 ng/mL) for 24 h before exposure to toxic insults. The Wnt-3a protein partially protected PC12 cells, with a 6-15% increase in cell viability in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wnt-3a; PC12 cells; Beta-catenin; NF-κB.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100010
Imagem não selecionada

Imprime registro no formato completo
Effects of propofol on lipopolysaccharide-induced expression and release of HMGB1 in macrophages BJMBR
Wang,T.; Wei,X.Y.; Liu,B.; Wang,L.J.; Jiang,L.H..
This study aimed to determine the effects of different concentrations of propofol (2,6-diisopropylphenol) on lipopolysaccharide (LPS)-induced expression and release of high-mobility group box 1 protein (HMGB1) in mouse macrophages. Mouse macrophage cell line RAW264.7 cells were randomly divided into 5 treatment groups. Expression levels of HMGB1 mRNA were detected using RT-PCR, and cell culture supernatant HMGB1 protein levels were detected using enzyme-linked immunosorbent assay (ELISA). Translocation of HMGB1 from the nucleus to the cytoplasm in macrophages was observed by Western blotting and activity of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the nucleus was detected using ELISA. HMGB1 mRNA expression levels increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propofol; HMGB1; LPS; RAW 264.7 cells; NF-κB.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400286
Imagem não selecionada

Imprime registro no formato completo
Galunisertib (LY2157299), a transforming growth factor-β receptor I kinase inhibitor, attenuates acute pancreatitis in rats BJMBR
Liu,X.; Yu,M.; Chen,Y.; Zhang,J..
Galunisertib (LY2157299), a selective ATP-mimetic inhibitor of TGF-β receptor I (TGF-βRI), is the only known TGF-β pathway inhibitor. In the present study, we investigated the effect of galunisertib on taurocholate (TAC)-induced acute pancreatitis (AP) in rats, and the role of TGF-β and NF-κB signaling pathways. AP was induced by infusion of TAC into the pancreatic duct of Sprague-Dawley male rats (n=30). The rats (220±50 g) were administered galunisertib intragastrically [75 mg·kg-1·day-1 for 2 days (0 and 24 h)]. Serum IL-1β, IL-6, TNF-α, amylase (AMY), lipase (LIP), and myeloperoxidase (MPO) levels were measured by ELISA. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA); NF-κBp65 and TGF-β1 proteins, as well as TGF-βRI and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute pancreatitis; TGFβ1; NF-κB; Galunisertib.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900705
Imagem não selecionada

Imprime registro no formato completo
Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity BJMBR
Planeta,C.S.; Lepsch,L.B.; Alves,R.; Scavone,C..
Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocaine; Apoptosis; NF-κB; CREB; BDNF; Neurotoxicity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100909
Imagem não selecionada

Imprime registro no formato completo
Plasma cytokine response, lipid peroxidation and NF-κB activation in skeletal muscle following maximum progressive swimming BJMBR
Oleto,A.F.; Sousa,L.P.; Barreto,T.O.; Cruz,J.S.; Penaforte,C.L.; Magalhães,J.C.; Sousa-Franco,J.; Pinto,K.M.C.; Campi-Azevedo,A.C.; Rocha-Vieira,E..
Our objective was to determine lipid peroxidation and nuclear factor-κB (NF-κB) activation in skeletal muscle and the plasma cytokine profile following maximum progressive swimming. Adult male Swiss mice (N = 15) adapted to the aquatic environment were randomly divided into three groups: immediately after exercise (EX1), 3 h after exercise (EX2) and control. Animals from the exercising groups swam until exhaustion, with an initial workload of 2% of body mass attached to the tail. Control mice did not perform any exercise but were kept immersed in water for 20 min. Maximum swimming led to reactive oxygen species (ROS) generation in skeletal muscle, as indicated by increased thiobarbituric acid reactive species (TBARS) levels (4062.67 ±1487.10 vs 19,072.48 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swimming; NF-κB; Interleukin-6; Interleukin-10; Thiobarbituric acid reactive species; Cytokine.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600008
Imagem não selecionada

Imprime registro no formato completo
Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal BJMBR
Huang,X.; Teng,Y.; Yang,H.; Ma,J..
Propofol is one of the most commonly used intravenous anesthetic agents during cancer resection surgery. A previous study has found that propofol can inhibit invasion and induce apoptosis of ovarian cancer cells. However, the underlying mechanisms are not known. miR-9 has been reported to be little expressed in ovarian cancer cells, which has been related to a poor prognosis in patients with ovarian cancer. Studies have also demonstrated that propofol could induce microRNAs expression and suppress NF-κB activation in some situations. In the present study, we assessed whether propofol inhibits invasion and induces apoptosis of ovarian cancer cells by miR-9/NF-κB signaling. Ovarian cancer ES-2 cells were transfected with anti-miR-9 or p65 cDNA or p65 siRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propofol; Ovarian cancer; MiR-9; NF-κB; Apoptosis; Invasion.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200605
Imagem não selecionada

Imprime registro no formato completo
Resveratrol Attenuates LPS-induced Apoptosis via Inhibiting NF- κ B Activity in Chicken Peripheral Lymphocyte Cultures Rev. Bras. Ciênc. Avic.
Zhang,CY; Huang,J; Kang,XT.
ABSTRACT This study aims to investigate whether resveratrol (RES) could inhibit NF-κB activity and protect in vitro cultured chicken lymphocytes from apoptosis induced by continuous LPS stimulation. Blood lymphocytes of chickens were collected and cultured. LPS was added to the samples of treatment group, while the same volume of normal saline (NS) was supplemented to those of the control group. In the treatment groups, four different concentrations of RES (0, 20, 40, and 80 µg/ml) were admixed. Then, the following indicators were tested: lymphocyte apoptotic rate, lymphocyte viability, NF-κB activity, TNF-a level, reactive oxygen species (ROS), and expression of apoptosis-related proteins (Fas/FasL and Caspase-8). The results indicated that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Resveratrol; NF-κB; Lymphocyte apoptosis; Chicken.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400747
Imagem não selecionada

Imprime registro no formato completo
Swertiamarin attenuates carbon tetrachloride (CCl4)-induced liver injury and inflammation in rats by regulating the TLR4 signaling pathway BJPS
Wu,Tao; Zhang,Qianrui; Song,Hongping.
The aim of the present study is to illustrate the effects of swertiamarin (STM), a natural iridoid from herbal medicines, on hepatic inflammation induced by carbon tetrachloride (CCl4) in rats. Male Sprague Dawley rats were exposed to CCl4 with or without STM co-administration for 8 weeks. Our results revealed that STM administration (100 and 200 mg/kg b.w.) significantly attenuated inflammation in livers of CCl4-treated rats. STM remarkably reduced the production of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), macrophage inflammatory protein-1a (MIP-1α), and monocyte chemotactic protein-1 (MCP-1) in liver tissue of CCl4-treated rats. In addition, STM treatment downregulated connective tissue growth factor (CTGF) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swertiamarin/effects; Carbon tetrachloride; Inflammation; TLR4; NF-κB.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400618
Imagem não selecionada

Imprime registro no formato completo
Therapeutic approaches for tumor necrosis factor inhibition BJPS
Barbosa,Maria Letícia de Castro; Fumian,Milla Machado; Miranda,Ana Luísa Palhares de; Barreiro,Eliezer J.; Lima,Lídia Moreira.
Tumor necrosis factor (TNF) consists of an inflammatory cytokine essential for homeostasis and organism defense. Despite its physiological relevance, both increased biosynthesis and release of TNF lead to the exacerbation of inflammatory and oxidative responses, which are related to the pathogenesis of a host of diseases of an inflammatory, autoimmune and/or infectious nature. In this context, effective therapeutic approaches for the modulation of TNF have been the focus of research efforts. Approximately one million individuals worldwide have been treated with biotechnological inhibitors of this cytokine, the so-called anti-TNF biopharmaceuticals. However, given the high risk of infection and the limitations related to cost and administration routes, new...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tumor necrosis factor; TACE; P38 MAPK; NF-κB; CAMP; Phosphodiesterase; Adenosine receptor.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300002
Imagem não selecionada

Imprime registro no formato completo
TNF-α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal BJMBR
Ren,Keyu; Yong,Chunming; Yuan,Hao; Cao,Bin; Zhao,Kun; Wang,Jin.
Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility is disturbed. Interstitial cells of Cajal (ICC) are required to maintain normal intestinal motility. In the present study, we assessed the effect of tumor necrosis factor-alpha (TNF-α) on viability and apoptosis of ICC, as well as on the expression of stem cell factor (SCF), ghrelin, and substance P. ICC were derived from the small intestines of Swiss albino mice. Cell viability and apoptosis were measured using CCK-8 assay and flow cytometry, respectively. ELISA was used to measure the concentrations of IL-1β, IL-6, ghrelin, substance P, and endothelin-1. Quantitative RT-PCR was used to measure the expression of SCF. Western blotting was used to measure the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TNF-α; SCF; Ghrelin; Substance P; NF-κB; Interstitial cells of Cajal.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600610
Registros recuperados: 13
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional