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Action of cobra venom on the renal cortical tissues: electron microscopic studies J. Venom. Anim. Toxins
RAHMY,T. R..
The effect of intramuscular (IM) injection of a sub-lethal dose of Naja haje cobra venom (0.015 mug/gm body weight) on the ultrastructure of renal cortical tissues of rabbits was studied at different time intervals after envenomation. Succinic dehydrogenase (SDH) enzyme activity was also detected in the renal cortical tubules. Three hours after venom injection, slight changes were seen in visceral cells and glomerular endothelia. The cortical tubular epithelia revealed an increase of lysosomal structures, cytoplasmic vacuolization, and nuclear irregularity. Severe ultrastructural changes were recorded 6 hours after envenomation, including hypertrophied parietal cells, blebbed visceral epithelial cells, and foot process disorganization. Dilated glomerular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Nephrotoxicity; Kidney; Ultrastructure.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100007
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Acute toxicity of commercial atrazine in Piaractus mesopotamicus: histopathological, ultrastructural, molecular, and genotoxic evaluation. Repositório Alice
PAIVA, P. P. de; DELCORSO, M. C.; MATHEUS, V. A.; QUEIROZ, S. C. do N. de; COLLARES-BUZATO, C. B.; ARANA, S..
The aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group. The genotoxicity was evaluated by micronucleus (MN) test in erythrocytes from peripheral blood. Qualitative and semi-quantitative histopathological analyses, and also...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brazilian ichthyofauna; Kidney lesions; Liver lesions; Micronucleus test; Pacu; Herbicida; Toxidez; Poluição da água; Piaractus mesopotamicus; Water pollution; Atrazine; Oxidative stress; Nephrotoxicity; Hepatotoxicity.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080546
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Amphotericin B-related nephrotoxicity in low-risk patients BJID
Berdichevski,Roberto Herz; Luis,Luciano Billodre; Crestana,Letícia; Manfro,Roberto Ceratti.
INTRODUCTION: Amphotericin B (AmphoB) is the drug of choice for treatment of severe fungal illnesses; however, it is very nephrotoxic. Modified (less toxic) amphotericins are very expensive. In low-risk patients, saline loading would be enough to prevent significant loss of renal function. MATERIAL AND METHODS: Patients with normal renal function and within the first 24 hours of treatment with AmphoB were prospectively enrolled in the study. Patients in intensive care units or who were using vasoactive drugs were excluded. Saline loads were infused before and after the AmphoB treatment. Blood and urine analyses were made at the beginning and at the end of the treatment. Serum creatinine was repeated 30 days after the end of the AmphoB treatment. RESULTS:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphotericin B; Acute renal failure; Nephrotoxicity; Sodium chloride.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200005
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Comparison of Amphotericin B Lipid Complex (Abelcet) and Liposomal Amphotericin B (Ambisome) on Rat Kidney: a Morphological Evaluation International Journal of Morphology
Mutluay,S. Deniz; Karakas,Pinar; Tasova,Yesim; Mete,Ufuk; Bozkir,M. Gülhal.
The aim of our study was to compare the nephrotoxic effects of liposomal Amphotericin B (Ambisome) and Amphotericin B lipid complex (Abelcet) on rat kidneys at short (14 days) and long term (28 days) treatment applications. Thirty-six male Wistar rats were included and divided into six groups (n=6). Groups 1 and 4 are composed as control groups by administrating intraperitoneal (ip) 0, 9 molar Serum physiologic for a period of 14 and 28 days respectively. Group 2 and 3 are treated with 5 mg/kg Ambisome and 5 mg/kg Abelcet for 14 days respectively, Group 5 and 6 are treated with same agents for 28 days respectively. Then, the rats were transcardially perfused, samples were taken from cortex and medulla regions of kidneys. The micrographs of group 1 and 4...
Tipo: Journal article Palavras-chave: Amphotericin B lipid formulations; Rat kidney; Nephrotoxicity; Ultrastructure.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200033
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Effects of sirolimus alone or in combination with cyclosporine A on renal ischemia/reperfusion injury BJMBR
Pereira,B.J.; Castro,I.; Burdmann,E.A.; Malheiros,D.M.A.; Yu,L..
Calcineurin inhibitors exacerbate ischemic injury in transplanted kidneys, but it is not known if sirolimus protects or exacerbates the transplanted kidney from ischemic injury. We determined the effects of sirolimus alone or in combination with cyclosporin A (CsA) on oxygenated and hypoxic/reoxygenated rat proximal tubules in the following in vitro groups containing 6-9 rats per group: sirolimus (10, 50, 100, 250, 500, and 1000 ηg/mL); CsA (100 µg/mL); sirolimus (50 and 250 ηg/mL) + CsA (100 µg/mL); control; vehicle (20% ethanol). For in vivo studies, 3-week-old Wistar rats (150-250 g) were submitted to left nephrectomy and 30-min renal artery clamping. Renal function and histological evaluation were performed 24 h and 7 days after ischemia (I) in five...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclosporin A; Nephrotoxicity; Renal proximal tubules; Renal ischemia.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800007
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Enzymatic and toxinological activities of Hypnale hypnale (hump-nosed pit viper) venom and its fractionation by ion exchange high performance liquid chromatography J. Venom. Anim. Toxins incl. Trop. Dis.
Tan,CH; Sim,SM; Gnanathasan,CA; Fung,SY; Ponnudurai,G; Pailoor,J; Tan,NH.
Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hypnale hypnale venom and venom fractions obtained using Resource Q ion exchange chromatography were determined. The venom exhibited toxic activities typical of pit viper venom, comparable to that of its sister taxon, the Malayan pit viper (Calloselasma rhodostoma). Particularly noteworthy were its high activities of thrombin-like enzyme, proteases, phospholipase A2, L-amino acid oxidase and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypnale hypnale; Venom; Enzymes; Toxins; Fibrinogen; Nephrotoxicity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400015
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Gentamicin Induces Renal Morphopathology in Wistar Rats International Journal of Morphology
de Souza,Vanessa Barboza; Oliveira,Rodrigo Fagundes L. de; Lucena,Hévio Freitas de; Ferreira,Aurigena A. A; Guerra,Gerlane Coelho Bernardo; Freitas,Maria de Lourdes; Queiroz,Karla Cristiane de Souza; de Araújo Júnior,Raimundo F.
Due to its prominent role in major excretory pathways, the kidney is particularly sensitive especially to toxicity for antimicrobials drugs. Storage of these drugs in the renal cortex, their effect on renal cells, have consequences on the renal function, and then reabsorbed by renal tubules induce nephrotixicity. Our objective was to show the renal morphopatological alterations induced by gentamicin through the histochemical methods of routine periodic acid de Schiff (PAS) staining and imunohistochemical staining for the expression of the protein P53, which is considered as a marker for cellular apoptosis. This allows the early detection of tubular lesions. The renal morphopathologic findings were cell apoptosis, basal membrane interruption, mesangial...
Tipo: Journal article Palavras-chave: Nephrotoxicity; Aminoglycosides; Expression of the P53 protein; Renal morphopatological alterations.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100011
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Influence of tetracycline in the hepatic and renal development of rat's offspring BABT
Machado,Ana Lourdes da Silva; Brandão,Adriana Aigotti Haberbeck; Silva,Cláudia Maria de Oliveira Monteiro da; Rocha,Rosilene Fernandes da.
This study aimed on evaluating the possible effects of tetracycline administered to rats on the tenth day of pregnancy, on kidney and liver development of their offspring. The liver showed vacuolization, necrosis, inflammation and sinusoidal dilatations, more evident in the newborn. Mitosis, early increase of Kupffer cells population and hipertrophy of hepatocytes with greater synthesis of glycogen were present on the tenth and twentieth days of life. The kidney showed slight tubular vacuolizations and necrosis, more proeminent in the newborn, as well as signs of tubular regeneration on the tenth and twentieth days. These results suggested that the organs studied went through several transitory morphological changes during development but presented signs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pregnancy; Development; Tetracycline; Hepatotoxicity; Nephrotoxicity.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100008
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Mesenchymal stem cell therapy promotes the improvement and recovery of renal function in a preclinical model Genet. Mol. Biol.
Urt-Filho,Antônio; Oliveira,Rodrigo Juliano; Hermeto,Larissa Correa; Pesarini,João Renato; David,Natan de; Cantero,Wilson de Barros; Falcão,Gustavo; Marks,Guido; Antoniolli-Silva,Andréia Conceição Milan Brochado.
Abstract Acute renal failure (ARF) is an extremely important public health issue in need of novel therapies. The present study aimed to evaluate the capacity of mesenchymal stem cell (MSC) therapy to promote the improvement and recovery of renal function in a preclinical model. Wistar rats were used as the experimental model, and our results show that cisplatin (5mg/kg) can efficiently induce ARF, as measured by changes in biochemical (urea and creatinine) and histological parameters. MSC therapy performed 24h after the administration of chemotherapy resulted in normalized plasma urea and creatinine levels 30 and 45d after the onset of kidney disease. Furthermore, MSC therapy significantly reduced histological changes (intratubular cast formation in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone marrow; Chemotherapy; Nephrotoxicity; Kidney disease; Tissue regeneration.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200290
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Nephrotoxicity during tenofovir treatment: a three-year follow-up study in a Brazilian reference clinic BJID
Pinto Neto,Lauro Ferreira da Silva; Bassetti,Bil Randerson; Fraga,Igor Hernandes Valvassoura; Oliveira Santos,Carolina Rocio; Ximenes,Paula Daher; Miranda,Angélica Espinosa.
Abstract In this study, 275 patients in use of tenofovir were retrospectively followed-up for three years to evaluate risk factors involved in impaired renal function. Analysis of variance (ANOVA) and Tukey's test were used to verify any differences in creatinine levels and estimated clearance at 0, 6, 12, 24 and 36 months, adjusting for the co-variables sex, skin color, age >50 years, arterial hypertension, diabetes and the use of the ritonavir-boosted protease inhibitors (PI/r) lopinavir/r or atazanavir/r. The software package STATISTICA 10® was used for statistical analysis. The patients’ mean age was 43.2 ± 10.7 years. Systemic arterial hypertension (SAH) and diabetes were found in 20.4% and 8.7% of the patients, respectively. Overall, 96.7% were on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tenofovir; Nephrotoxicity; Impaired renal function; HIV.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100014
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Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand J. Venom. Anim. Toxins incl. Trop. Dis.
Charoenpitakchai,Mongkon; Wiwatwarayos,Kulachet; Jaisupa,Nattapon; Rusmili,Muhamad Rusdi Ahmad; Mangmool,Supachoke; Hodgson,Wayne C.; Ruangpratheep,Chetana; Chanhome,Lawan; Chaisakul,Janeyuth.
Abstract Background: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats. Methods: Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Krait; Bungarus candidus; Myotoxicity; Nephrotoxicity; Venom; Kidney.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100304
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Protection against cephalosporin-induced lipid peroxidation and nephrotoxicity by (+)-cyanidanol-3 and vitamin E BJMBR
Cojocel,C.; Tolle,K.-L.; El-Hajj,H.; Baumann,K..
The ability of the clinically used cephalosporins: cephalothin, cefotaxime and cefotiam to induce lipid peroxidation (LPO) and renal damage was compared to that of nephrotoxic cephaloridine under in vivo conditions. Glutathione was measured in rat liver or in renal cortex as non-protein sulfhydryls. LPO was measured in plasma, renal cortex and liver by the generation of malondialdehyde or as the increase in renal cortical concentration of conjugated dienes. Impairment of renal function was measured as the decrease in renal cortical accumulation of the organic anion p-aminohippurate (PAH). Administration of cephalosporins to rats as a single dose (2000 mg/kg, ip) induced a significant glutathione-depletion in the renal cortex with cephaloridine, and in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cephalosporins; Glutathione depletion; Lipid peroxidation; Nephrotoxicity; Antioxidants; Vitamin E.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600017
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RENAL MORPHOMETRY OF FETUSES RATS TREATED WITH CADMIUM International Journal of Morphology
Roman,T. R. N.; de Lima,E. G.; Azoubel,R; Batigália,F.
Cadmium is a heavy metal which is found in the soil, air and food. It is present in everyday life and currently it is being implicated as a toxic, teratogenic and carcinogenic agent. It causes lesions to several organs of humans and animals alike. This work aims to investigate the toxic effects on fetuses of dams exposed to cadmium chloride treatment. Ten pregnant rats were divided into two groups. Five rats, the study group, received an intraperitoneal administration of cadmium chloride at 5 mg/Kg body weight on the 10th day of pregnancy and the other five, the study group, received a solution of 0.85% sodium chloride at the same dose and at the same application site. The fetuses together with the placentas and umbilical cords were collected on the 20th...
Tipo: Journal article Palavras-chave: Cadmium; Nephrotoxicity; Karyometry.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000300010
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Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats BJPS
Erhirhie,Earnest Oghenesuvwe; Ilodigwe,Emmanuel Emeka.
This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dryopteris filix mas Leaf Extract; Sub-chronic toxicity; Acute toxicity; Nephrotoxicity; Hepatotoxicity; Dryopteris/toxicity; Toxicity tests/utilization; Ethanol/ toxicity..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100543
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Tepoxalin on renal function and liver enzymes in cats exposed to hypotension with isoflurane Ciência Rural
Freitas,Gabrielle Coelho; Carregaro,Adriano Bonfim; Bisetto,Shayne Pedrozo; Barbosa,Aline Soares; Fantinato Neto,Paulo; Dellova,Deise Carla Almeida Leite; Lopes,Carlize.
This study aimed to evaluate the possible renal and hepatic toxicity of tepoxalin administered before or after isoflurane-induced hypotension, as well as for five consecutive days. Twelve healthy mixed-breed cats, adult males, weighing 4.0±0.8kg were allocated into two groups. They received 25mgkg-1 of tepoxalin orally, two hours before the anesthetic procedure (PRE) or after the procedure (POST) and daily for five days. Cats were anesthetized with isoflurane and the concentration was increased until mean arterial pressure reached 40-60mmHg and kept at this level for 60 minutes. During hypotension, the physiological variables were measured at time 0 and every 10 minutes until 60 minutes, and bleeding time was measured at time 0, 30 and 60 minutes. Blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-steroidal anti-inflammatory drug; Nephrotoxicity; Hepatotoxicity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000600020
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Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom BJMBR
Martins,A.M.C.; Nobre,A.C.L.; Almeida,A.C.; Bezerra,G.; Lima,A.A.M.; Fonteles,M.C.; Monteiro,H.S.A..
Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha), we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml) in the absence and presence of thalidomide (15 µM) or pentoxifylline (500 µM) for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml) was tested on an isolated perfused rat kidney (N = 6 for each group). The first 30 min of each experiment were used as control. The supernatant was added to the perfusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nephrotoxicity; Crotalus durissus cascavella; Pentoxifylline; Thalidomide; Macrophage.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000012
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The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats BJMBR
The aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. The chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bence Jones proteins; Scintigraphic study; Multiple myeloma; Nephrotoxicity.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700008
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Tribulus terrestris Hydroalcoholic Extract Effect on Cisplatin-Induced Apoptosis in Mice Kidney International Journal of Morphology
Ghanbari,Ali; Zare,Fatemeh; Khazaei,Mozafar; Moradi,Mojtaba; Raoofi,Amir.
Cisplatin (EBEWE Pharma, Unterach, Austria) is an anti-cancer drug used in chemotherapy. One of the limiting major side effects of cisplatin is nephrotoxicity. Tribulus terrestris (TT) has been used as an synthetic or herbal protective agents for kidney disorders. The present study amid to investigate the Tribulus terrestris Hydroalcoholic extract effect on cisplatin-induced apoptosis in mice kidney. Male adult mice (n= 30) were divided into control group and 4 experimental groups (n= 6). Control group received saline, the first experimental group received cisplatin (5.5 mg/kg) and other three experimental groups received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg i.p) respectively. The kidneys were...
Tipo: Journal article Palavras-chave: Cisplatin; Nephrotoxicity; Tribulus terrestris; Kidneys; Protective effect.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200046
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Urinary NO3 excretion and renal failure in indinavir-treated patients BJMBR
Eira,M.; Araujo,M.; Seguro,A.C..
Treatment with indinavir (IDV), a protease inhibitor, is frequently associated with renal abnormalities. We determined the incidence of renal failure (creatinine clearance <80 mL min-1 1.73 (m²)-1) in HIV patients treated with highly active antiretroviral therapy, including IDV, and investigated the possible mechanisms and risk factors of IDV nephrotoxicity. Thirty-six patients receiving IDV were followed for 3 years. All were assessed for age, body weight, duration of infection, duration of IDV treatment, sulfur-derivative use, total cholesterol, triglycerides, magnesium, sodium, potassium, creatinine, and urinalysis. We also determined renal function in terms of creatinine clearance, urine osmolality and fractional excretion of sodium, potassium, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV; Indinavir; Nephrotoxicity; Nitric oxide.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800009
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