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Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov BJM
Visnuvinayagam,Sivam; K,Thangavel; N,Lalitha; S,Malmarugan; Sukumar,Kuppannan.
Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle disease; Komarov strain; Chicken embryo fibroblast; Newcastle disease vaccine.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300861
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Control of Newcastle Disease in Village Chickens AgEcon
Centre for International Economics.
Between 1983 and 1992, the Australian Centre for International Agricultural Research (ACIAR) invested A$3 million in research to find a vaccine that could provide protection from Newcastle disease in chickens and be applied in village environments in developing countries. A further $160 000 was invested in follow up projects which ended in 1996. Village chickens often provide the only source of protein to poor villagers living in remote areas and Newcastle disease frequently devastates unvaccinated village flocks. The ACIAR-sponsored research was highly successful in developing a heat resistant vaccine (HRV4) which could be readily used in the field by coating it onto chicken feed. The vaccine was commercialised by an Australian company which subsequently...
Tipo: Book Palavras-chave: Newcastle disease; Chickens; Poultry; Vaccine; Malaysia; Vietnam; ACIAR; Australia; Developing country; Village; Africa; Farm Management; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Development; Livestock Production/Industries; Research and Development/Tech Change/Emerging Technologies; Research Methods/ Statistical Methods.
Ano: 1998 URL: http://purl.umn.edu/47190
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Effect of associated vaccines on the interference between Newcastle disease virus and infectious bronchitis virus in broilers Rev. Bras. Ciênc. Avic.
Cardoso,WM; Aguiar Filho,JLC; Romão,JM; Oliveira,WF; Salles,RPR; Teixeira,RSC; Sobral,MHR.
The phenomenon of viral interference between live vaccines against Newcastle Disease and infectious bronchitis has been reported since the 50's and many researchers have reported its prejudicial effects on avian immunization. Therefore, this study evaluated the effect of associated vaccines on the interference between Newcastle disease virus (NDV) and infectious bronchitis virus (IBV) in broilers. There were 400 broiler chicks divided into five groups. The groups were submitted to mono or polyvalent vaccinations against IBV and NDV, except for the non-vaccinated control group (CG). Sera were collected at 35 and 45 days of age and submitted to serologic tests to assess antibody levels. It was observed the occurrence of interference in the immune response...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious bronchitis; Newcastle disease; Vaccines; Viral interference.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000300008
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Effect of Dietary Inclusion of Sodium Bicarbonate on Digestibility of Nutrients and Immune Response in Caged Layers During the Summer Rev. Bras. Ciênc. Avic.
Abbas,G; Ahmad,F; Saeed,M; Ayasan,T; Mahmood,A; Yasmeen,R; Kamboh,A.
ABSTRACT The aim of this project was to investigate the effect of dietary inclusion of sodium bicarbonate (NaHCO3) on nutrient digestibility and immune response of caged layers during summer when the temperature exceeds 40 ˚C. For immune response trial, White Leghorn layers (n=160; 24 weeks old) were purchased from a poultry farm and were divided into five treatment/diets groups (4 replicate/treatment). Diet A, was without NaHCO3 and served as control whereas, diets B, C, D, and E contained 0.5, 1.0, 1.5 and 2.0% NaHCO3, respectively. All these birds were vaccinated against Newcastle disease (ND) virus at the start of the experiment and thereafter with one-month intervals. Blood samples were collected from two birds/replicate at 10 days post vaccination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat stress; Layers; Newcastle disease; Nutrient digestibility; Sodium bicarbonate.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200339
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Effect of vitamin E levels on the cell-mediated immunity of broilers vaccinated against coccidiosis Rev. Bras. Ciênc. Avic.
Silva,ICM da; Ribeiro,AML; Canal,CW; Vieira,MM; Pinheiro,CC; Gonçalves,T; de Moraes,ML; Ledur,VS.
Studies on the relationships between animal nutrition and immunity have sought reliable methodologies to measure responses. Cell-mediated immune response is similarly studied in humans. The cutaneous basophil hypersensitivity test (CBH) is one of the methods to measure that response and consists in the infiltration of inflammatory cells, particularly of lymphocytes and basophils, as result of the application of substances capable of inducing cell proliferation in determined sites, such as wings, wattle, and interdigital space in birds. CBH is considered a simple and fast method and can be applied in birds of different ages. In immunocompetence studies with poultry, phytohemagglutinin-P (PHA-P) is a commonly used substance, despite the variability of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Coccidiosis; Cell-mediated immune response; Newcastle disease; Vaccine; Vitamin E.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000100008
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Evaluation of the Viral Interference between Lentogenic Newcastle Disease Virus (Lasota) and Avian Influenza Virus (H9N2) using Real-Time Reverse Transcription Polymerase Chain Reaction in SPF Chicken Rev. Bras. Ciênc. Avic.
Fazel,P; Mehrabanpour,MJ.
ABSTRACT Lentogenic Newcastle disease virus (lNDV) such as Lasota strain and low pathogenicity avian influenza such as H9N2 virus are two of the most economically important viruses affecting poultry worldwide, and little attention in recent years has been paid to simultaneous infections in chickens with these two viruses for the reason that co-infection do occur but are not easily detected. In the present study, chickens were inoculated with lNDV (Lasota) and LPAIV (A/chicken/Tehran/ZMT-173/99(H9N2)) simultaneously or sequentially three days apart. Oropharyngeal and cloacal swabs were collected from chickens from 1 to 14 days after inoculation. RRT-PCR for AIV and NDV detection was performed. The rate of viral shedding was measured within 14 days. No...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle disease; Influenza virus (H9N2); Viral interference.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300537
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Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks BABT
Ashraf,Asma; Shah,Muhammad Salah-ud-Din; Habib,Mudasser; Hussain,Mujahid; Mahboob,Shahid; Al-Ghanim,Khalid.
ABSTRACT Newcastle disease (ND) is a major infectious disease of the poultry caused by a virulent strain of Avian Paramyxovirus - 1, that is a single strand non-segmented negative sense RNA virus. ND virus is major threat to the poultry industry in many countries of the world. The study was aimed to isolate and identify Newcastle disease virus (NDV) by using a haemagglutination inhibition (HI) test and reverse transcription-polymerase chain reaction (RT-PCR) assay. A total 100 samples of infected and dead birds were collected from different poultry farms. The weight of the birds was ranged 1000-1200g. The birds were divided into 3 groups. Haemagglutination assay (HA) was performed to detect the presence of NDV in suspension of infected homogenized tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle disease; Epidemic; Molecular characterization; Avian virus; RT-PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100332
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LAYERS '99, PART I: REFERENCE OF 1999 TABLE EGG LAYER MANAGEMENT IN THE U.S. AgEcon
The National Animal Health Monitoring System (NAHMS) is sponsored by the USDA:APHIS:Veterinary Services (VS). The NAHMS Layers '99 Study was designed to provide information about the nation's layer population for education and research purposes. The USDA's National Agricultural Statistics Service (NASS) collaborated with VS to select a statistically-valid sample from 15 states for Layers '99. The 15-state target population accounted for over three-quarters of the table egg layers in the U.S. as of December 1, 1998. The study was limited to companies with 30,000 or more layers. While the majority of farm sites were contract farms (61.0 percent), the majority of birds were located on company owned farms (72.6 percent), indicating that company owned farms...
Tipo: Report Palavras-chave: NAHMS; Layer; Layers; Poultry; Pullet; Flock; Egg; Table egg; Lay; Molt; Cage; Contract farm; Coccidiosis; Newcastle disease; Infectious bronchitis; Salmonella enteritidis; S.e.; Competitive exclusion; Laryngotracheitis; LT; Mycoplasma gallisep; Livestock Production/Industries.
Ano: 1999 URL: http://purl.umn.edu/32757
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Mycoplasma synoviae infection on Newcastle disease vaccination of chickens BJM
Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia; Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus), previously...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033
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Newcastle disease in white Pekin ducks: response to experimental vaccination and challenge Rev. Bras. Ciênc. Avic.
Nishizawa,M; Paulillo,AC; Nakaghi,LSO; Nunes,AD; Campioni,JM; Doretto Júnior,L.
A total of 120 Pekin ducks were distributed at random into four experimental groups, vaccinated or not against Newcastle disease (ND): G1 (Ulster 2C strain), G2 (B1 strain), G3 (LaSota strain), and G4 (nonvaccinated group). At 60 days of age, all groups were challenged with a pathogenic ND virus (NDV) suspension, and a group of specific pathogen free (SPF) chicks (G5) was also inoculated. Cloacal and tracheal swabs from all birds were collected after six, 14, 20, and 30 days post-challenge for virus isolation. NDV was isolated in 100% of SPF chicks. Pekin ducks from all groups, vaccinated or not, did not show any ND clinical signs, demonstrating that these birds are not susceptible to ND clinical disease. In the control group (G4), the virus was isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anas platyrhynchos; NDV carrier; Newcastle disease; Pekin duck; Vaccination.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200008
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Survey of pathogens in threatened wild red-tailed Amazon parrot (Amazona brasiliensis) nestlings in Rasa Island, Brazil BJM
Vaz,Frederico Fontanelli; Serafini,Patrícia Pereira; Locatelli-Dittrich,Rosangela; Meurer,Rafael; Durigon,Edison Luiz; Araújo,Jansen de; Thomazelli,Luciano Matsumiya; Ometto,Tatiana; Sipinski,Elenise Angelotti Bastos; Sezerban,Rafael Meirelles; Abbud,Maria Cecília; Raso,Tânia Freitas.
ABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian influenza virus; Chlamydia psittaci; Microbiology; Newcastle disease; West Nile virus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400747
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The use of scaning electron microscopy in postvaccinal evaluation of tracheal epithelium of Coturnix coturnix japonica Ciência Rural
Santin,Elizabeth; Lima,Fabiana S.; Paulillo,Antônio C.; Nakaghi,Laura S. O.; Maiorka,Alex.
This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 <FONT FACE=Symbol>¾</FONT> control birds (non-vaccinated); T2 <FONT FACE=Symbol>¾</FONT> birds vaccinated with Ulster 2C strain; t3 <FONT FACE=Symbol>¾</FONT> birds vaccinated with B1 strain; t4 <FONT FACE=Symbol>¾</FONT> birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coturnix coturnix japonica; Newcastle disease; Scanning electron microscopy; Respiratory post-vaccinal reaction.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000100019
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