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Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas ArchiMer
Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc.
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total...
Tipo: Text Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00239/35010/33544.pdf
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Identification of molecular markers for oocyte competence in bovine cumulus cells. Repositório Alice
MELO, E. O.; CORDEIRO, D. M.; PELLEGRINO, R.; WEI, Z.; DAYE, Z. J.; NISHIMURA, R. C.; DODE, M. A. N..
bitstream/item/167130/1/Melo-et-al-2017-Animal-Genetics.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Microarray; Oocyte quality; Cattle; Gene expression.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080229
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Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas ArchiMer
Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie.
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and...
Tipo: Text Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf
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