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Registros recuperados: 39
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A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax ArchiMer
Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois.
Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using...
Tipo: Text Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf
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Acclimation of juvenile Mugil liza Valenciennes, 1836 (Mugiliformes: Mugilidae) to different environmental salinities Neotropical Ichthyology
Lisboa,Viviana; Barcarolli,Indianara F.; Sampaio,Luís A.; Bianchini,Adalto.
Survival and physiological parameters associated with metabolism and osmoregulation were evaluated in juveniles of the Lebranche mullet Mugil liza acclimated to different water salinities (5, 10, 20, 30, and 40‰) for 15 days. Room temperature (25ºC) and photoperiod (12L:12D) were fixed. Fish were fed twice-a-day with commercial diet (28% crude protein) until satiation. After acclimation, whole body oxygen consumption was measured and fish were euthanized and sampled for blood, gills, and liver. Whole body oxygen consumption and plasma osmolality did not change in the range of salinities tested. The isosmotic point was estimated as 412.7 mOsmol kg-1 (13.5‰). Gill Na+,K+-ATPase activity tended to be lower at 20 and 30‰, while liver glycogen content was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Glycogen; Na+; K+-ATPase; Osmoregulation; Oxygen consumption.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300591
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Accumulation of organic and inorganic solutes in NaCl-stressed sorghum seedlings from aged and primed seeds Scientia Agricola
Oliveira,Alexandre Bosco de; Alencar,Nara Lídia Mendes; Prisco,José Tarquinio; Gomes-Filho,Enéas.
Although it has been known that the seed's physiological potential affects its response to osmoconditioning and abiotic stresses, researches involving seed aging and priming associated to abiotic stresses are scarce. The aim of this work was to evaluate the role of seed priming on salt tolerance in sorghum seedlings from seeds with two vigor levels (aged or non-aged) and to verify the organic and inorganic solute contributions as osmoregulators in NaCl-stressed seedlings from aged and primed seeds. The combinations of two seed vigor levels (aged or not), two seed types (primed or not) and two salinity levels (exposed to NaCl at 100 mM or not) were evaluated. In low physiological quality seeds (aged seeds), priming provided an attenuation of salinity's...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorghum bicolor; Accelerated aging; Priming; Salinity; Osmoregulation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600004
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Activity determination of Na+ K+ - ATPase and Mg++ - ATPase enzymes in the gill of Poecilia vivipara (Osteichthyes, Cyprinodontiformes) in different salinities BABT
Amaral,Marcelo da Cunha; Bonecker,Ana Cristina Teixeira; Ortiz,Cláudio H. D..
This work aimed to know the tolerance mechanisms through the salinity variation by Na+ K+ - ATPase and Mg++ - ATPase and enzymes encountered in the gills of Poecilia vivipara. In field, the presence of this species was observed in salinities of 0 and 28‰. In laboratory, these fish were maintained in aquarium with mean salinity of 30‰ and positive responses were obtained. Some adult specimens, collected in a lagoon of the Coqueiros Beach, were utilized as matrixes. In the experiments the specimens used were those born in the test aquarium. For each salinity studied three replicates were made with three specimens for each one. The alevins were maintained in salinities of 5, 10, 15, 20, 25, 30 and 35‰ during a month for adaptation. Gills were extracted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poecilia vivipara; Na+ K+ ATPase; Mg++ ATPase; Osmoregulation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100001
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Adaptations biochimiques intestinales chez la truite: Influence de l'aliment et de la salinite sur quelques proprietes membranaires ArchiMer
Leray, C.
The authors report the results of physiological and biochemical experiments which confirm the important role player by fish (Salmo gairdneri ) intestinal mucosa in their osmoregulatory process. The ability of the dietary lipids to modulate this function is described. It was demonstrated that the ionic apical permeability of the enterocytes are related to their membrane lipid composition. The importance of the essential fatty acids is emphasized. The time course changes of the apical permeability are of value in improving sea water adaptation, similar conclusions are reported for Nacl supplementation.
Tipo: Text Palavras-chave: Salmonidae; Pisces; Biological membranes; Fatty acids; Diets; Osmotic adaptations; Biochemical phenomena; Intestines; Lipids; Nutrition; Salinity effects; Euryhalinity; Osmoregulation.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1249.pdf
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An economic evaluation of the osmoregulation gene technology to the Australian wheat industry AgEcon
Farquharson, Robert J.; Morgan, J.M.; Brennan, John P..
Episodes of rainfall irregularity and soil moisture deficit have focused attention on the widespread limitation of water supply on winter cereal crop production in Australia. This has motivated a number of efforts at breeding for improved drought tolerance. A recent example involves a cellular adaptation which mitigates water loss through solute accumulation (osmoregulation or osmotic adjustment). An assessment of the performance of osmoregulation yield response in the presence of climate change found that wheat cultivars with this gene are unlikely to be adversely affected by hotter and drier conditions across the wheat belt. The results of an economic evaluation of potential future innovations (wheat cultivars) from the osmoregulation gene technology are...
Tipo: Conference Paper or Presentation Palavras-chave: Economic evaluation; Wheat breeding; Osmoregulation; Crop Production/Industries; Research Methods/ Statistical Methods.
Ano: 2004 URL: http://purl.umn.edu/58446
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Could a diet enriched with n-3 highly unsaturated fatty acids be considered a promising way to enhance the immune defences and the resistance of Penaeid prawns to environmental stress? ArchiMer
Chim, Liet; Lemaire, Pierrette; Delaporte, Maryse; Le Moullac, Gilles; Galois, Robert; Martin, Jean-louis.
The prawn Penaeus stylirostris (Stimpson), when fed for 28 days with n-3 highly unsaturated fatty acid (HUFA)-enriched feed pellets, demonstrated an enhanced resistance to variations in environmental parameters (a decrease in temperature and salinity over a 4-day period from 28 degreesC to 17 degreesC and from 35 parts per thousand to 10 parts per thousand respectively) and an improvement in their immune defence potential, i.e. increased agglutination titre of plasma and increased respiratory burst of haemocytes.
Tipo: Text Palavras-chave: Immunostimulation; Osmoregulation; Environment; Stress; Essential fatty acids; Crustaceans.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-606.pdf
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Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum ArchiMer
Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Grousset, Evelyse; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine.
V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite,...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+-ATPase; V-type H+-ATPase; Gills; Branchiostegite.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00202/31352/37901.pdf
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Digestive tract ontogeny of Dicentrarchus labrax: Implication in osmoregulation ArchiMer
Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R.
The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf
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Dissolved oxygen and ammonia levels in water that affect plasma ionic content and gallbladder bile in silver catfish Ciência Rural
Becker,Alexssandro Geferson; Garcia,Luciano de Oliveira; Kochhann,Daiani; Gonçalves,Jamile Fabbrin; Loro,Vania Lúcia; Baldisserotto,Bernardo.
Ionic contents (Na+, K+ and Cl-) of plasma and gallbladder bile (GB) of juveniles silver catfish, Rhamdia quelen (156.1±0.2g, 28.2±0.3cm), were determined in three different times (0, 6 and 24h) after exposure to: a) control or high dissolved oxygen (DO = 6.5mg L-1) + low NH3 (0.03mg L-1); b) low DO (3.5mg L-1) + low NH3; c) high DO + high NH3 (0.1mg L-1); and d) low DO + high NH3. High waterborne NH3 or low DO levels increased plasma and GB ion levels. These parameters might have followed different mechanisms to affect osmoregulation since a synergic effect of these variables was detected.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen compound; Hypoxia; Ion levels; Jundiá; Osmoregulation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600020
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Effects of salt stress on plant growth, stomatal response and solute accumulation of different maize genotypes Braz. J. Plant Physiol.
Azevedo Neto,André Dias de; Prisco,José Tarquinio; Enéas-Filho,Joaquim; Lacerda,Claudivan Feitosa de; Silva,José Vieira; Costa,Paulo Henrique Alves da; Gomes-Filho,Enéas.
Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 and ICI8447) were sown in vermiculite, and after germination they were transplanted into nutrient solution or nutrient solution containing 100 mmol.L-1 of NaCl and placed in a greenhouse. During the experimental period plant growth (dry matter, shoot to root dry mass ratio, leaf area, relative growth rate, and net assimilation rate), leaf temperature, stomatal conductance, transpiration, predawn water potential, sodium, potassium, soluble amino acids and soluble carbohydrate contents were determined in both control and salt stressed plants of all genotypes studied. Salt stress reduced plant growth of all genotypes but the genotypes BR5033 and BR5011 were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth analysis; Osmoregulation; Salinity; Transpiration; Zea mays; Water relations.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100005
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Evaporative water loss and dehydration during the night in hummingbirds RChHN
HARTMAN BAKKEN,BRADLEY; SABAT,PABLO.
Nectar-feeding birds oscillate between avoiding overhydration when they are feeding and preventing dehydration during fasts. Here, we examined how resting rates of total evaporative water loss (TEWL) and metabolic water production (MWP) influence water balance in the green-backed firecrown (Sephanoides sephanoides), a Chilean hummingbird. We hypothesized that a circadian rhythm in TEWL would assuage the dehydration risk that hummingbirds face during the night. However, we did not find support for this idea. In resting hummingbirds, rates of TEWL during the day (54 ±6 µLh-1,n = 8) and night (65 ± 12 h-1, n = 5) were similar. Rates of MWP were also similar between the day (22 ±3 µL h-1, n = 8) and night (23 ± 2 µL h-1, n = 5). MWP rates were significantly...
Tipo: Journal article Palavras-chave: Circadian rhythm; Evaporative water loss; Hummingbird; Osmoregulation; Water balance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000300001
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Growth and production components of West Indian cherry cultivated with saline waters and potassium fertilization AGRIAMBI
Lima,Geovani S. de; Pinheiro,Francisco W. A.; Dias,Adaan S.; Gheyi,Hans R.; Soares,Lauriane A. dos A.; Silva,Saulo S. da.
ABSTRACT Due to water limitations in terms of both quantity and quality in the semi-arid region of northeastern Brazil, the use of waters with high concentrations of salts become necessary in irrigated agriculture. Thus, this study aimed to evaluate the growth and production of grafted West Indian cherry under saline water irrigation and potassium fertilization. The experiment was carried out in drainage lysimeters under protected environment conditions, using a Regolithic Neosol with sandy loam texture. Treatments resulted from the combination of two levels of electrical conductivity - ECw (0.8 and 3.8 dS m-1) of irrigation water and four doses of potassium (50, 75, 100 and 125% of the recommendation of Musser), arranged in a randomized block design, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malpighia emarginata; Salt stress; Osmoregulation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662019000400250
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IMPROVING THE TOLERANCE OF Vicia faba AGAINST ENVIRONMENTAL SALINITY RESULTED FROM THE IRRIGATION WITH SEA WATER BY USING KNO3 AND (NH4)2SO4 AS CHEMICAL OSMOREGULATORS Acta biol.Colomb.
MOHAMED,KAMEL.
The familiar solutes, Na+, K+, Ca2+, Mg2+, Cl-, PO4(3-), SO4(2-), soluble carbohydrates, amino acids and soluble proteins, which play a role in osmotic adjustment were estimated to investigate the role of potassium nitrate and ammonium sulphate as osmoregulators and their effects on the solutes composition. Vicia faba L. was cultivated and irrigated with 5, 10, 15 and 20 % (v:v) sea water. The plants were divided to three groups. The first was irrigated with sea water only. The second was treated with 5 mM KNO3 while the third was treated with 5mM (NH4)2SO4. The plants were left to grow until flowering stage. The results indicated that the non treated group increased the soluble carbohydrates in the roots to avoid the influx of sodium. The treatment with...
Tipo: Journal article Palavras-chave: Osmoregulation; Irrigation; Salinity; Sea water; Vicia faba.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000200006
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Incidence de la salinite sur les equilibres de distribution hydrique et ioniques et sur le metabolisme respiratoire dans le muscle blanc, le muscle rouge et la branchie chez le muge Chelon labrosus ArchiMer
Gallis, J; Piquemal, S; Lambert, J; Sergent, P; Rousseau, V.
Oxygen consumption, water, K super(+) and Na super(+) contents were measured in white muscle, red muscle and gill epithelium in the mullet Chelon labrosus) in relation with the duration of fresh water or sea water (1500mOsm) adaptation. The unit respiratory activities in gill and red muscle where respectively 5 and 3 timesmore elevated than in white muscle. The pericellular K super(+)/Na super(+) ratio of the 3 tissues decreased with the fresh water adaptation duration. In the gill, the intracellular K super(+)/Na super(+) ratio was stable while the parameter decreased strongly in the white muscle. The results are discussed in relation to energetic cost of homocellular regulation and homoepithelial regulation.
Tipo: Text Palavras-chave: Chelon labrosus; Mugilidae; Pisces; Gills; Muscles; Oxygen consumption; Respiration; Ions; Water balance; Osmoregulation; Salinity effects.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1251.pdf
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Ion fluxes in silver catfish (Rhamdia quelen) juveniles exposed to different dissolved oxygen levels Neotropical Ichthyology
Rosso,Felipe Link de; Bolner,Keidi C. S.; Baldisserotto,Bernardo.
Low dissolved oxygen levels in the water (hypoxia) can be provoked by oxygen consumption by fish and other organisms, organic matter decomposition, phytoplankton blooms, and temperature increase. The objective of the present study was to investigate Na+, Cl-, K+, and ammonia fluxes in silver catfish (Rhamdia quelen) exposed to different dissolved oxygen levels. Juveniles (9 ± 1g) maintained at 6.0 mg.L-1 dissolved oxygen were transferred to four 40 L aquaria with different dissolved oxygen levels (in mg.L-1): 6.0, 4.5, 3.5, and 2.5. In another series of experiments, juveniles were acclimated at 6.0 or 2.5 mg.L-1 dissolved oxygen levels, and then placed in two 40 L aquaria with 6.0 mg.L-1 dissolved oxygen. For both series of experiments, 1, 24, 48 or 120 h...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxygen saturation; Osmoregulation; Jundiá.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000400007
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Ion uptake pathways in European sea bass Dicentrarchus labrax ArchiMer
Blondeau-bidet, Eva; Hiroi, Junya; Lorin-nebel, Catherine.
Ion uptake mechanisms are diverse in fish species, certainly linked to duplication events that have led to the presence of a multitude of paralogous genes. In fish, Na+ uptake involves several ion transporters expressed in different ionocyte subtypes. In the European sea bass Dicentrarchus labrax, several key transporters potentially involved in Na+ uptake have been investigated in seawater (SW) and following a 2 weeks freshwater (FW) acclimation. Using gel electrophoresis, we have shown that the Na+/H+-exchanger 3 (nhe3, slc9a3) is expressed in gills and kidney at both salinities. Quantitative realtime PCR analysis showed a significantly higher nhe3 expression in fresh water (FW) compared to SW. Its apical localization in a subset of gill ionocytes in...
Tipo: Text Palavras-chave: Teleost; Osmoregulation; Ion transporters; Na+ uptake; NHE3; NCC2.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59618/62638.pdf
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Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction Neotropical Ichthyology
Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A..
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009
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Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
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Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase ArchiMer
Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf
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