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Registros recuperados: 125
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A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Baron, S; Sharbel, Tim; Sauvage, Christopher; Boudry, Pierre.
The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae...
Tipo: Text Palavras-chave: Oysters; Aquaculture; Parental assignment; High throughput; Larval DNA extraction; Multiplex.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1814.pdf
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A long term study of bonamiosis in Quiberon bay, France ArchiMer
Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf
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A methology for the stocks assessments of cultivated oysters among along the French Atlantic coasts ArchiMer
Bacher, Cedric; Baud, Jean-pierre; Bodoy, Alain; Deslous-paoli, Jean-marc; Dreno, Jean-paul; Heral, Maurice; Maurer, Daniele; Prou, Jean.
An assessment of the reared stocks of the Pacific oyster (Crassostrea gigas ) has been recently conducted on the major French areas of molluscs culture. The methodology proposed included both estimates of densities obtained by subsampling in the field, and aerial photographs which covered the cultivated, intertidal area. The size and the number of samples were designed to reach a precision of 10% on the final assessment in each area. The field work consisted in weighing and counting the oyster in unit areas of 0.5 m2 for the bottom culture or in trays or bags, for the off bottom technique. These results give a better knowledge of the technique evolution and of the general structures in the cultivated areas.
Tipo: Text Palavras-chave: Modeling; Estimation; Stocks; Crassostrea gigas; Oysters.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-2864.pdf
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A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae , Vibrio parahaemolyticus and Vibrio vulnificus in seafoods ArchiMer
Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C..
Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also...
Tipo: Text Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf
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Absence de captage de Crassostrea gigas dans le bassin de Marennes-Oléron en 1981 : causes et conséquences ArchiMer
Berthome, Jean-paul; Deslous-paoli, Jean-marc; Heral, Maurice; Razet, Daniel; Garnier, Jacqueline.
The want of C. gigas spat-catching in the Marennes-oleron basin in 1981 can be due to various different reasons which are analysed here. Physico -chemical and trophic factors that can induce variations in the quality of gametogenesis, are successively in comparison with the year 1979 when reproduction was good. The biochemical evolution and a study of gonads slow a lack in maturation in 1981 even during the summer period. Late maturation of gonads bring about physiological consequences which induced mortality of cultivated oysters at the end of winter. A feeble recruit and a decrease of the cultivated stock have economic consequences which here sketched up.
Tipo: Text Palavras-chave: Larvae; Recrutment; Spat; Crassostrea gigas; Oysters; Larves; Recrutement; Captage; Crassostrea gigas; Huîtres.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/acte-2948.PDF
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Abundance and distribution of Lithophaga (Mytilidae) in extant and fossil oysters: taphonomic and paleobiological implications Ameghiniana
Mauna,Cecilia; Casadío,Silvio; Parras,Ana; Pascual,Marcela.
In this study we analyze the abundance and distribution patterns of Lithophaga patagonica in valves of Ostrea puelchana and compare them to those of Lithophaga sp. observed on the fossil species " Ostrea " patagonica and " Ostrea " alvarezii from the late Miocene Puerto Madryn Formation. No specimen of the fossil oysters showed borings of Lithophaga sp. on the interior surface of the valves. This suggests that they were produced while the oysters were still living and, at the same time, that the oyster beds were buried rapidly after death. In Ostrea puelchana the boring abundance was significantly higher for the left valve and, within it, the areas more heavily bored were the umbones and the platform. The same results were obtained for valves of " Ostrea "...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lithophaga; Bioerosion; Oysters; Taphonomy; Miocene; Argentina.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0002-70142005000200010
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Activité respiratoire en continu de l'huître Crassostrea gigas : mise au point d'une nouvelle méthodologie ArchiMer
Bougrier, Serge; Geairon, Philippe.
A continuous monitoring system to measure the respiration of Crassostrea gigas has been developped. The metabolic function estimated by oxygen consumption has been monitored during periods of 24 h, without food, at a temperature constant of 15°C. Preliminary results showed that the mean percentage of oyster activities was 50 % with a high variability from 0 to 100 %. The number of activity periods per day (24 h) is function of the oysters with variation between 0 and 7.
Tipo: Text Palavras-chave: Physiology; Respiration; Crassostrea gigas; Oysters; Physiologie; Respiration; Crassostrea gigas; Huîtres.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3046.pdf
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Aide à l'estimation des stocks d'huîtres en élevage par télédétection basse altitude: étude de faisabilité ArchiMer
De Pontual, Helene.
Existing methods to assess the biomass of reared oyster's stocks are ail based on aerial photographs and field data. In this study, we propose an automatic processing of the digitized aerial photographs making use of advanced vision tools such as mathematical morphology. The implemented a1gorithms are described. They give enough convincing results to suggest further developments. However, this method requires the use of digital images. To this respect, a part of this paper deals with data acquisition problems. We particularly study the feasibility of using airborne video recording system. Such device should give a quite accurate information, easier to process than the classical aerial photographs.
Tipo: Text Palavras-chave: Huître; Estimation des stocks; Photographie aérienne; Vidéo aéroportée; Analyse d'images numériques; Oysters; Stock assessment; Aerial pholographs; Airborne videography; Digital image processing.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00105/21669/19247.pdf
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Amylase polymorphism affects growth in the cupped oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Samain, Jean-francois; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Van Wormhoudt, A..
The better understanding of physiological and environmental factors that determine optimal food conversion efficiencies is of major interest for the cupped oyster Crassostrea gigas for which the strong increase of aquaculture has been correlated in France with a decrease in productivity due to competition between aquatic species for limited food supplies at grow-out sites. To investigate the non-neutrality of the polymorphism of amylase, a key enzyme for carbohydrate assimilation, in oyster physiology, we report in the present paper the growth and survival measurement of amylase genotypes within five bi-parental C. gigas families, bred to be polymorph at the two PCR-RFLP amylase markers, and reared over one year under standard culture conditions in two...
Tipo: Text Palavras-chave: Amylase markers; Growth; Amylase Polymorphism; Genetic; Oysters; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3311.pdf
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Analysis of the Economic Effects of Requiring Post-harvest Processing for Raw Oysters AgEcon
Muth, Mary K.; Viator, Catherine; Karns, Shawn A..
Tipo: Conference Paper or Presentation Palavras-chave: Oysters; Post-harvest processing; Hydrostatic pressure; Cool pasteurization; GIS; Agricultural and Food Policy; Food Consumption/Nutrition/Food Safety; L510; Q180; Q220.
Ano: 2011 URL: http://purl.umn.edu/103814
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Anomalies de croissance de la coquille de Crassostrea gigas dans le bassin de Marennes -Oléron. Bilan de toris années d'observation ArchiMer
Heral, Maurice; Berthome, Jean-paul; Polanco Torres, E.; Alzieu, Claude; Deslous-paoli, Jean-marc; Razet, Daniel; Garnier, Jacqueline.
The appearance and changes of the oyster shell's gelatinous bags have been monitored since 1979. The increase in thickness that causes the growth into a "ball" can be explained by an overproduction of gel. The various environmental factors that can intervene in the secretion of gel are successively examined.
Tipo: Text Palavras-chave: Oysters; Shell; Crassostrea gigas; Huîtres; Coquille; Crassostrea gigas.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2943.pdf
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Apport de la génétique pour une meilleure connaissance des populations naturelles et une amélioration des populations cultivées d'huîtres plates et creuses ArchiMer
Lapegue, Sylvie.
In the framework of the French diploma "Habilitation à Diriger des Recherches", Sylvie Lapègue's synthesis of research includes ten years (1998-2007) of work in bivalve genetics, and mainly cupped and flat oysters. This research has been developed in (1) flat oyster population genetics at the European level and cupped oyster populations and species genetics at a global scale, (2) the characterization of a cytogenetic particularity observed in oysters that corresponds to a high rate of aneuploidy in somatic cells, and (3) more recently in the use of molecular markers for the construction or improvement of genetic maps, allowing (4) the identification of areas of the genome related to the resistance to summer mortality in the Pacific oyster and the...
Tipo: Text Palavras-chave: Ostrea; Crassostrea; Oysters; QTLs; Genetic maps; Breeding; Aneuploidy; Population genetics; Ostrea; Crassostrea; Huître; QTLs; Cartes génétiques; Amélioration; Aneuploïdie; Génétique des populations.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-3478.pdf
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Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture ArchiMer
Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf
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Approche de la capacité trophique d'un écosystème conchylicole ArchiMer
Heral, Maurice; Deslous-paoli, Jean-marc; Prou, Jean.
After a short bibliographical study of the different approaches for determination of the trophic capacity of a bay for molluscs culture, the authors, with a global mode, show the relations between the growth performance of the oysters Crassostrea gigas in relation with the cultivated stocks. The volumes of consumed and assimilated food are calculated for the stocks in relation with the incoming volumes of water in the bay and the residence time for neap tide and spring tide. The authors find a decrease of growth rate in relation with the volume of consumed food. When it is above 60% and when the volume of assimilated food is above 20%, there is a large decrease of the growth of oysters.
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Stock assessment; Models; Food consumption; Growth; Biomass; Stock cultivé; Biomasse; Croissance; Modèle; Crassostrea gigas; Huîtres; Capacité trophique.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/acte-3834.PDF
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Aspects biométriques et biochimiques de la croissance de l'huître Crassostrea virginica en élevage suspendu à Solomons (Baie de Chesapeake) ArchiMer
Goulletquer, Philippe; Prou, Jean; Heral, Maurice.
Growth and proximate biochemical composition of the American oyster Crassostrea virginica were studied in the Patuxent river (Maryland), located in the middle reach of the Chesapeake bay (U.S.A.). The effects of stocking densities and environmental variables (depth, temperature) were examined in 1989-1990 on oyster population by using off-bottom culture. No significant density effect was observed on the shell growth, which is described by using a seasonally oscillating function. In contrast, the meat growth and the proximate biochemical composition were bath affected by the stacking density and the depth location. The variability of the resulting effects was observed based on a seasonal trend. Different hypothesis mainly related tO environmental conditions...
Tipo: Text Palavras-chave: USA; Chesapeake Bay; Oysters; Growth; Biochemical composition; Crassostrea virginica; Croissance; USA; Chesapeake Bay; Huîtres; Biochimie; Biométrie; Crassotrea virginica.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3076.pdf
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Assessments of natural mortality of cultivated oysters (Crassostrea gigas) in the bay of Marennes-Oleron (France) ArchiMer
Bodoy, Alain.
The natural mortality of oysters could by considered, in a man controlled ecosystem, as the result of the action of biological factors, and human factors other then the harvest of the population. Such mortalities need to be estimated in order to be used in population dynamics, as a tool for management purposes. ln Marennes-Oleron bay, different ways of estimating the natural mortality are available. Several oyster farms were monitored for assessing growth, mortality and production in rearing conditions. Furthermore, an experimental framework which includes 15 stations gave an appraisal of the same factors under controlled conditions. Finally, another estimate will be soon available by comparin9 the number of recruited oysters, and the number of oyster...
Tipo: Text Palavras-chave: Marennes Oleron; Mortality; Crassostrea gigas; Oysters.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-2962.pdf
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Bilan des connaissance et des enjeux relatifs aux ressources marines de l'estuaire de l'Odet ArchiMer
Billy, Stéphany; Peronnet, Isabelle.
The estuary of the Odet, which can be defined as a ria, is an integral part of SAGE, taken on by SIVALODET. This space was hardly studied and it seemed important to perform a full assessment of knowledge and issues relating to this ria's fragile and unstable ecosystem. This assessment must be performed according to several points of focus; it must first of all strive to retrace the history of the fisheries in the Odet, then make it possible to identify as clearly as possible the marine habitats that make up this site. With that, it must then bring to light the potential resources of the estuary and finally resituate the Odet in relation to the other Breton estuaries.
Tipo: Text Palavras-chave: Water quality; Shellfish farming; Parasite; Oysters; Bonamia ostrea; Marteilia refringens; Odet estuary; Brittany; Parasite; Huître; Qualité de l'eau; Habitats de l'Odet; Nourriceries de l'Odet; Marteilia refringens; Bonamia ostrea; Réglementation de la pêche; Estuaire de l'Odet; Bretagne.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-2334.pdf
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Bonamiasis : a model study of diseases in marine molluscs ArchiMer
Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne.
Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!.
Tipo: Text Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf
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Breeding and Domestication of Eastern Oyster ( Crassostrea virginica ) Lines for Culture in the Mid-Atlantic, Usa: Line Development and Mass Selection for Disease Resistance ArchiMer
Frank-lawale, Anu; Allen, Standish K.; Degremont, Lionel.
A selective breeding program for Crassostrea virginica was established in 1997 as part of an initiative in Virginia to address declining oyster harvests caused by the two oyster pathogens Haplosporidium nelsoni (MSX) and Perkinsus marinus (Dermo). Housed in the Aquaculture Genetics and Breeding Technology Center (ABC), the objective of the program was to develop and disseminate disease-resistant lines that would enable an oyster culture industry. Today, culture of disease-resistant cultivars accounts formore than 90%of oyster production in the state, where 28.1 million half-shell oysters and 2 billion eyed larvae were sold in 2012. Results of our line development program as of 2006 are reported. Eight ABC lines from 3 genetic groups—East Coast (EC),...
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Selective breeding; Disease resistance; Chesapeake Bay; Growth; Genetic improvement; MSX; Dermo.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29950/28395.pdf
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Caractérisation de marqueurs génétiques fonctionnels de la nutrition et/ou de l'adaptation (les amylases) chez l'huître creuse Crassostrea gigas : intérêts pour la sélection ArchiMer
Prudence, Marie.
Two amylase genes, A and B, from the oyster Crassostrea gigas were characterized. Using PCR-RFLP, 6 and 4 alleles, respectively, were described for the amylase genes A and B. The roles of A and B amylase genes were investigated experimentally. They are expressed during larval and adult stages, and A transcripts are more abundant than B. The A transcript increases significantly with temperature, in high trophic conditions. However, A and B transcript levels do not change when food quantity increases although amylase activity augments. The level of B is correlated with dietary starch quantities, whereas the amount of A appears to remain constant ; simultaneously amylase activity decreases and the KM increases. These results suggest that expression of B is...
Tipo: Text Palavras-chave: Enzymatic analyse; Genetic polymorphism; Genes; Nutrition; Growth; Environmental effects; Oysters; Amylases; Amylases; Analyse enzymatique; Polymorphisme génétique; Gènes; Nutrition; Croissance; Effets de l'environnement; Huîtres; Amylases; Amylases.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1700.pdf
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