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A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES Acta biol.Colomb.
OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Journal article Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
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Assessment of genetic diversity in Indian ash gourd (Benincasa hispida) accessions using RAPD markers National Institute of Agronomic Research
Pandey, S.; Kumar, S.; Rai, M.; Mishra, U.; Singh, M..
Thirty four (9 cultivars and 25 germplasm) genotypes of ash gourd (Benincasa hispida) have been studied to assess the molecular diversity using RAPD markers. A total of 25 RAPD markers produced 163 amplicons, of which 73 % were found polymorphic indicating a high degree of diversity. RAPD based dendrogram showed dissimilarity values from 0.64 to 0.943 also suggesting that the accessions represent a genetically diverse population. The accession IVAG-223 was clearly separated from rest of the accessions based on the RAPD primers. The dendogram revealed that the accessions from northeastern regions of India are different from the accessions of other parts of India, as they cluster together. On the basis of clustering, it was indicated that cultivars had a...
Tipo: Conference Paper Palavras-chave: Genetic resources; Genetic diversity; Dendrogram; PCR amplification; Germplasm; Molecular diversity.
Ano: 2008 URL: http://hdl.handle.net/2174/193
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Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak Biol. Res.
CÓRDOVA,JOSÉ LUIS; ASTORGA,JOSEFA; SILVA,WALLY; RIQUELME,CARLOS.
Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first report of this bacterium causing an epidemic in Chile. V. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. These isolates were analyzed by polymerase chain reaction (PCR) amplification of the pR72H gene, a species-specific sequence. Based on the pR72H gene amplification pattern, at least three different isolates of V. parahaemolyticus were found. Two isolates (named amplicons A and C) generated PCR products...
Tipo: Journal article Palavras-chave: Bacterial bloom; Bacterial geographical dispersion; PCR amplification; Molecular typification.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017
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